Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 9 | 9 | 5 | 6 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 2 | 1 | 0 | 10 | 7 | 8 | 5 | 10 | 6 | 4 | 0 | 4 | 7 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 129 | 442 | 81 | 82 | 86 | 126 | 129 | 154 | 129 | 118 | 108 | 64 | 116 | 240 | 212 | 241 | 235 | 180 | 121 | 422 | 94 | 74 | 209 | 206 |
Endoplasmic Reticulum | 1 | 11 | 5 | 6 | 5 | 14 | 21 | 25 | 29 | 26 | 17 | 16 | 3 | 3 | 5 | 12 | 15 | 11 | 1 | 6 | 3 | 1 | 6 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 2 | 1 | 4 | 7 | 10 | 5 | 6 | 7 | 4 | 0 | 0 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 11 | 3 | 5 | 8 | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 9 | 5 | 4 | 2 | 7 | 1 | 1 | 0 | 2 | 3 | 1 | 0 | 9 | 0 | 3 | 3 | 2 |
Unique Cell Count | 130 | 447 | 84 | 89 | 91 | 143 | 160 | 196 | 171 | 159 | 137 | 101 | 118 | 243 | 218 | 246 | 238 | 185 | 126 | 447 | 101 | 85 | 228 | 225 |
Labelled Cell Count | 130 | 456 | 88 | 92 | 96 | 162 | 177 | 215 | 193 | 172 | 151 | 114 | 121 | 248 | 226 | 262 | 257 | 199 | 126 | 447 | 101 | 85 | 228 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 118.0 | 85.2 | 60.6 | 53.1 | 37.1 | 35.6 | 29.1 | 28.3 | 25.3 | 26.9 | 24.8 | 22.5 | 52.8 | 50.3 | 46.6 | 83.6 | 101.8 | 95.7 | 57.3 | 59.8 | 59.1 |
Std Deviation (1e-4) | 23.1 | 18.6 | 21.7 | 20.8 | 13.5 | 14.5 | 12.0 | 11.1 | 9.6 | 9.5 | 8.3 | 9.0 | 16.8 | 19.3 | 18.6 | 30.1 | 35.6 | 39.8 | 22.8 | 23.7 | 22.5 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.71 | -0.77 | -1.06 | -1.1 | -1.26 | -1.17 | -1.29 | -1.43 | -0.2 | -0.27 | -0.38 | 0.46 | 0.75 | 0.66 | -0.08 | -0.02 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -0.6 | -2.1 | -3.4 | -3.7 | -4.1 | -4.3 | -3.6 | -5.4 | 0.8 | 1.4 | 0.4 | 0.8 | 1.3 | 0.4 |
Endoplasmic Reticulum | 0.2 | 0 | 1.0 | 1.7 | 1.7 | 2.4 | 2.3 | 1.6 | 2.1 | 0 | 0 | 0 | 0 | 0.1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 80.4418 | 104.1793 | 96.3464 | 94.7704 | 99.853 | 95.4871 | 104.5026 | 133.0912 | 122.2913 | 102.3742 | 109.2013 | 115.0115 | 93.474 | 111.9561 | 104.38 | 105.07 | 97.4032 | 101.8531 |
Actin | 0.004 | 0.0009 | 0.0114 | 0.0004 | 0.001 | 0.0017 | 0.0027 | 0.0014 | 0.0042 | 0.0036 | 0.0012 | 0.0025 | 0.0101 | 0.0006 | 0.0043 | 0.0004 | 0.0005 | 0.0018 |
Bud | 0.0016 | 0.0007 | 0.0025 | 0.0019 | 0.0041 | 0.0024 | 0.0029 | 0.0014 | 0.0027 | 0.0026 | 0.0067 | 0.0039 | 0.0028 | 0.0007 | 0.0028 | 0.0011 | 0.0026 | 0.0008 |
Bud Neck | 0.0011 | 0.001 | 0.0014 | 0.001 | 0.0012 | 0.0014 | 0.0009 | 0.0007 | 0.0014 | 0.0028 | 0.0022 | 0.0036 | 0.0013 | 0.0008 | 0.0018 | 0.0007 | 0.0014 | 0.0018 |
Bud Periphery | 0.0042 | 0.0006 | 0.0019 | 0.0015 | 0.0017 | 0.0036 | 0.0048 | 0.0006 | 0.0039 | 0.0026 | 0.026 | 0.0047 | 0.0039 | 0.0004 | 0.0029 | 0.0008 | 0.0022 | 0.0004 |
Bud Site | 0.0026 | 0.0005 | 0.0029 | 0.0002 | 0.0004 | 0.0004 | 0.0012 | 0.0005 | 0.002 | 0.007 | 0.0009 | 0.0005 | 0.0017 | 0.0005 | 0.0058 | 0.0002 | 0.0003 | 0.0002 |
Cell Periphery | 0.0336 | 0.0103 | 0.0282 | 0.0292 | 0.0033 | 0.0101 | 0.013 | 0.0083 | 0.0158 | 0.0082 | 0.0041 | 0.0059 | 0.021 | 0.011 | 0.0194 | 0.0244 | 0.0025 | 0.0029 |
Cytoplasm | 0.5417 | 0.8754 | 0.705 | 0.5319 | 0.6253 | 0.6752 | 0.7337 | 0.9128 | 0.7929 | 0.5842 | 0.7589 | 0.7463 | 0.6428 | 0.8919 | 0.7746 | 0.6456 | 0.7797 | 0.7725 |
Cytoplasmic Foci | 0.0144 | 0.0018 | 0.0068 | 0.0027 | 0.0082 | 0.004 | 0.011 | 0.002 | 0.005 | 0.005 | 0.0096 | 0.0056 | 0.0031 | 0.0011 | 0.0051 | 0.0024 | 0.0234 | 0.0153 |
Eisosomes | 0.0011 | 0 | 0.0009 | 0.0001 | 0 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0964 | 0.0472 | 0.0238 | 0.0099 | 0.012 | 0.1396 | 0.0536 | 0.017 | 0.0254 | 0.0262 | 0.0105 | 0.0392 | 0.1114 | 0.0277 | 0.0509 | 0.0098 | 0.0134 | 0.0956 |
Endosome | 0.0211 | 0.0045 | 0.0146 | 0.0193 | 0.038 | 0.0243 | 0.0127 | 0.0045 | 0.011 | 0.0603 | 0.0303 | 0.0345 | 0.0143 | 0.0026 | 0.017 | 0.0177 | 0.0194 | 0.0324 |
Golgi | 0.0078 | 0.0019 | 0.0023 | 0.0012 | 0.0021 | 0.0085 | 0.0026 | 0.0011 | 0.0034 | 0.009 | 0.0027 | 0.0072 | 0.0028 | 0.0008 | 0.0054 | 0.0007 | 0.0011 | 0.0071 |
Lipid Particles | 0.0084 | 0.0006 | 0.004 | 0.0016 | 0.002 | 0.0008 | 0.003 | 0.0005 | 0.0015 | 0.0018 | 0.0015 | 0.0003 | 0.0019 | 0.0005 | 0.002 | 0.0017 | 0.0025 | 0.0009 |
Mitochondria | 0.0165 | 0.0007 | 0.0052 | 0.0015 | 0.0007 | 0.0066 | 0.0057 | 0.0008 | 0.0051 | 0.0061 | 0.0135 | 0.006 | 0.0107 | 0.0005 | 0.0061 | 0.0004 | 0.0136 | 0.0016 |
None | 0.0057 | 0.0002 | 0.0053 | 0.0002 | 0.0003 | 0.0009 | 0.0051 | 0.0005 | 0.0045 | 0.0012 | 0.0095 | 0.0006 | 0.0083 | 0.0003 | 0.0026 | 0.0003 | 0.0127 | 0.0009 |
Nuclear Periphery | 0.0457 | 0.0036 | 0.011 | 0.0091 | 0.0151 | 0.0104 | 0.0236 | 0.0024 | 0.0067 | 0.0163 | 0.007 | 0.0186 | 0.0313 | 0.0119 | 0.0101 | 0.0093 | 0.0043 | 0.0109 |
Nucleolus | 0.0022 | 0.0001 | 0.0086 | 0.0002 | 0.0002 | 0.009 | 0.0023 | 0.0002 | 0.0015 | 0.0005 | 0.0045 | 0.0003 | 0.0006 | 0.0001 | 0.0014 | 0.0007 | 0.0018 | 0.0002 |
Nucleus | 0.0091 | 0.0016 | 0.0062 | 0.0035 | 0.0042 | 0.0117 | 0.0064 | 0.0018 | 0.0034 | 0.0074 | 0.0052 | 0.0401 | 0.0072 | 0.0024 | 0.0041 | 0.0032 | 0.0037 | 0.0041 |
Peroxisomes | 0.0065 | 0 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0006 | 0 | 0.0007 | 0.0005 |
Punctate Nuclear | 0.001 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0032 | 0.0001 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0004 | 0 | 0.0004 | 0.0081 |
Vacuole | 0.1344 | 0.0428 | 0.1243 | 0.2672 | 0.2023 | 0.0608 | 0.0895 | 0.0399 | 0.0869 | 0.2047 | 0.0739 | 0.056 | 0.0971 | 0.0416 | 0.0697 | 0.2193 | 0.0791 | 0.0342 |
Vacuole Periphery | 0.0407 | 0.0055 | 0.0303 | 0.1173 | 0.0778 | 0.0284 | 0.0209 | 0.0035 | 0.0224 | 0.0495 | 0.0307 | 0.024 | 0.0266 | 0.0041 | 0.0129 | 0.0611 | 0.0346 | 0.0079 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1227.0099 | 1153.4672 | 1343.8782 | 1452.8401 | 1606.507 | 1144.977 | 1145.8325 | 1161.419 | 1287.8639 | 1232.0724 |
Translational Efficiency | 1.9107 | 2.141 | 1.8894 | 1.6414 | 1.7999 | 2.0512 | 1.8572 | 1.9939 | 1.6933 | 1.7465 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|