Standard name
Human Ortholog
Description Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.05 0.05 0.07 0 0.05 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0.06 0.15 0.19 0.15 0.16 0.21 0.05 0.1 0 0.08 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0.05 0 0.06 0.1 0.08
Mitochondria 0.82 0.68 0.71 0.79 0.89 0.85 0.87 0.78 0.89 0.9 0.94 0.91 0.74 0.48 0.44 0.08 0.07 0.06 0.67 0.56 0.68 0.62 0.57 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.08 0.05 0.05 0.09 0.07 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.06 0.05 0 0 0.09 0.11 0.09 0.1 0.09 0.08 0.07 0 0 0 0.06 0.06 0.1 0 0 0 0 0 0
Peroxisomes 0.12 0.16 0.32 0.27 0.14 0.05 0.05 0.13 0 0 0 0 0.2 0.3 0.33 0.39 0.42 0.35 0.09 0.06 0.16 0.05 0.07 0.07
SpindlePole 0.07 0.11 0.11 0.07 0.05 0 0 0.05 0 0 0 0 0.12 0.24 0.25 0.31 0.3 0.28 0.06 0.11 0.05 0.06 0.05 0.06
Vac/Vac Membrane 0 0.11 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.09 0.14 0.14 0.2 0 0 0 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 5 0 0 2 0 0 0 1 0 0 0 1 0 1 0 6 2 4 5 10 16
Bud 7 9 13 12 9 15 22 19 15 38 26 25 2 8 8 5 8 9 1 3 2 3 7 7
Bud Neck 0 2 0 0 0 0 1 0 1 1 0 0 1 3 0 1 4 3 1 2 2 0 1 1
Bud Site 2 2 1 0 3 4 10 5 0 6 9 10 0 0 1 5 2 14 – – – – – –
Cell Periphery 1 3 1 5 1 1 4 5 4 5 7 2 1 6 3 10 7 4 0 0 0 0 0 0
Cytoplasm 2 0 3 3 3 8 5 7 8 5 3 4 6 9 12 11 12 15 0 0 0 0 2 1
Endoplasmic Reticulum 0 1 0 1 0 0 0 1 0 1 1 0 0 1 1 2 2 4 0 0 0 0 0 0
Endosome 2 12 15 4 7 3 3 8 1 1 1 0 10 57 61 30 41 49 11 17 8 14 25 26
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 37 18 5 8 2 7 9 4 11 32 31
Mitochondria 147 118 181 138 238 269 449 339 406 662 464 563 121 182 139 16 19 13 140 101 174 116 190 213
Nucleus 0 1 1 0 2 4 4 4 1 4 0 7 1 1 3 9 5 12 0 0 0 0 0 0
Nuclear Periphery 9 1 4 2 4 24 27 21 40 51 28 38 2 2 3 2 3 1 3 0 0 0 0 2
Nucleolus 17 11 12 6 9 30 59 37 45 68 39 44 6 9 5 11 16 24 0 0 0 0 2 3
Peroxisomes 21 27 83 47 38 15 24 57 8 29 10 19 32 112 103 77 108 83 18 11 41 9 24 28
SpindlePole 12 20 28 13 13 10 22 22 10 22 9 12 19 89 79 61 77 67 12 20 13 10 17 20
Vac/Vac Membrane 6 19 7 2 9 13 10 7 15 9 12 11 9 48 27 28 37 48 0 5 0 9 14 18
Unique Cell Count 180 174 256 175 267 316 518 432 456 734 493 619 163 376 315 198 260 236 209 182 258 188 335 378
Labelled Cell Count 226 229 350 238 336 396 642 532 554 902 610 735 210 564 464 273 350 348 209 182 258 188 335 378


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 8.2 8.9 8.8 9.0 8.0 7.7 8.1 8.1 7.6 7.3 7.7 9.5 9.8 8.9 9.0 9.0 8.7 9.7 10.7 11.5
Std Deviation (1e-4) 2.4 1.9 1.8 2.0 1.8 2.2 2.0 1.9 2.0 1.9 2.0 2.1 2.2 1.9 1.7 2.4 2.5 2.3 1.9 2.4 2.3
Intensity Change (Log2) – – – -0.02 0.02 -0.16 -0.21 -0.14 -0.13 -0.23 -0.29 -0.21 0.09 0.14 0.0 0.01 0.02 -0.03 0.12 0.27 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.8 -1.0 -0.2 -0.5 -0.4 -1.2 0.1 0.1 -0.7 -2.1 -2.0 -1.6 -1.4 -1.2 -0.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 2.0 2.7 2.3 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.8 -1.8 -3.3 -4.6 -2.8 -4.9 0 -5.1 0 0.1 3.6 4.7 3.3 3.6 4.9
Golgi 0 0 0 0 0 0 0 0 0 0 4.9 3.5 0 0 0
Mitochondria 1.9 5.3 4.2 5.4 2.3 6.2 7.6 8.8 7.6 0.8 -5.6 -6.4 -13.4 -14.8 -14.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2
Nuclear Periphery 0 0 3.3 2.4 2.2 3.8 3.2 2.6 2.9 0 0 0 0 0 0
Nucleolus -0.6 -0.8 2.2 3.0 1.9 2.4 2.3 1.7 1.3 -0.5 -1.6 -2.2 0.4 0.7 2.3
Peroxisomes -1.2 -4.9 -8.7 -10.5 -6.1 -11.8 -12.4 -12.0 -12.3 -2.9 -0.7 0.1 1.4 2.1 0.6
SpindlePole -1.2 -2.6 -3.7 -3.6 -2.9 -5.0 -5.0 -5.5 -5.8 0.2 4.0 4.3 5.3 5.3 4.9
Vacuole 0 0.4 0.9 -0.7 -1.0 0.4 0 -0.2 -0.9 1.5 4.4 2.9 4.5 4.7 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.0103 11.7157 13.1775 11.9409 12.398 12.6651 11.3085 15.3677 13.7393 11.7318 14.3744 12.8486 11.5551 12.7196 10.5042 10.3247 9.1608 10.3513
Actin 0.0878 0.0043 0.0148 0.0003 0.001 0.0012 0.0182 0.0038 0.0078 0.0212 0.0003 0.0005 0.0045 0.0009 0.0155 0.0028 0.0128 0.0052
Bud 0.0013 0.0003 0.0003 0 0.0001 0 0.0005 0.0001 0.0001 0.0012 0 0 0.0001 0 0.0002 0.0001 0.0003 0
Bud Neck 0.0005 0.0004 0.0002 0 0 0 0.0003 0.0001 0.0002 0.0007 0 0 0.0001 0 0.0001 0 0.0001 0
Bud Periphery 0.0031 0.0006 0.0005 0 0.0003 0 0.001 0.0003 0.0001 0.0018 0.0001 0 0.0002 0.0001 0.0004 0.0003 0.001 0.0001
Bud Site 0.0015 0.0008 0.0016 0 0.0001 0 0.0024 0.0003 0.0007 0.003 0 0 0.0002 0.0001 0.0003 0 0.0003 0.0001
Cell Periphery 0.0002 0.0001 0.0003 0 0.0001 0 0.0003 0.0006 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0.0002 0
Cytoplasm 0.0002 0 0.0001 0 0 0 0.0004 0 0.0001 0.0002 0 0 0 0 0 0 0.0001 0
Cytoplasmic Foci 0.0081 0.0085 0.0042 0.0009 0.0002 0.0002 0.0093 0.0041 0.0014 0.0064 0.0001 0 0.0037 0.0004 0.0027 0.0006 0.0046 0.0011
Eisosomes 0.0006 0.0002 0.0011 0.0001 0.0001 0.0001 0.0006 0.0003 0.0002 0.0004 0.0001 0.0001 0.0004 0.0001 0.0003 0.0003 0.0005 0.0002
Endoplasmic Reticulum 0.0008 0 0.0001 0 0 0 0.0005 0.0002 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0 0.0011 0
Endosome 0.0048 0.0008 0.0063 0.0068 0.001 0.0004 0.0121 0.0067 0.0049 0.0092 0.001 0.0003 0.0018 0.0039 0.0033 0.0019 0.0242 0.0012
Golgi 0.0122 0.0028 0.004 0.037 0.0017 0.0082 0.016 0.027 0.0141 0.0129 0.0014 0.001 0.0059 0.0302 0.0126 0.0194 0.0369 0.0066
Lipid Particles 0.0195 0.0701 0.0091 0.0026 0.0005 0.0082 0.0252 0.0123 0.0024 0.0167 0.001 0.0025 0.0685 0.0046 0.0145 0.0034 0.0113 0.0195
Mitochondria 0.8358 0.8758 0.9411 0.9497 0.9926 0.9779 0.8807 0.8948 0.9552 0.9138 0.9928 0.9933 0.8943 0.9444 0.9429 0.968 0.8727 0.9592
None 0.0003 0 0.0001 0 0 0 0.0009 0 0 0.0002 0 0 0.0001 0 0.0001 0 0.0004 0
Nuclear Periphery 0.0007 0 0.0002 0 0 0 0.0016 0.0031 0 0.0004 0 0 0.0003 0.0002 0.0003 0 0.0157 0.0001
Nucleolus 0.0002 0.0003 0.0003 0 0 0 0.0006 0.0014 0.0001 0.0003 0 0 0.0004 0.0001 0.0001 0 0.0002 0.0001
Nucleus 0.0001 0.0001 0.0001 0 0 0 0.0005 0.0002 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0 0.0004 0
Peroxisomes 0.0183 0.0275 0.0078 0.0017 0.0009 0.0026 0.0204 0.0083 0.0023 0.0047 0.0004 0.0003 0.0149 0.0018 0.0036 0.0016 0.0107 0.0042
Punctate Nuclear 0.0005 0.0001 0.0003 0 0 0 0.0017 0 0 0.0004 0 0 0.0001 0 0.0002 0 0.0002 0.0001
Vacuole 0.0007 0.0007 0.0027 0.0002 0.0004 0.0001 0.0025 0.011 0.0032 0.0016 0.0005 0.0001 0.0008 0.0037 0.0006 0.0003 0.0012 0.0003
Vacuole Periphery 0.0029 0.0064 0.0046 0.0007 0.0011 0.001 0.0046 0.0251 0.0069 0.0042 0.0022 0.0017 0.0035 0.0092 0.0019 0.001 0.0053 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.642 6.6797 9.3626 11.0021 9.1499 14.9363 13.5078 13.9285 13.087 13.9925
Translational Efficiency 0.8724 0.8339 0.6711 0.5442 0.7625 0.3975 0.6766 0.6244 0.7236 0.576

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Cem1

Cem1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available