Standard name | |
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Human Ortholog | |
Description | DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 2 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 13 | 7 | 7 | 8 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 1 | 2 | 3 | 8 | 8 | 2 | 10 | 10 | 6 | 1 | 1 | 3 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 173 | 363 | 351 | 265 | 168 | 305 | 331 | 242 | 254 | 282 | 228 | 213 | 144 | 204 | 210 | 176 | 191 | 182 | 165 | 346 | 328 | 178 | 155 | 202 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 4 | 6 | 1 | 5 | 15 | 9 | 12 | 0 | 0 | 2 | 8 | 6 | 4 | 0 | 2 | 0 | 1 | 1 | 1 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 2 |
Golgi | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5 | 1 | 1 | 4 | 12 | 10 | 19 | 10 | 47 | 17 | 19 | 25 | 0 | 0 | 0 | 7 | 1 | 3 | 1 | 1 | 5 | 1 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 6 | 15 | 11 | 11 | 21 | 8 | 15 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 3 | 1 | 4 | 3 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 0 | 2 |
Vac/Vac Membrane | 4 | 8 | 10 | 13 | 14 | 70 | 97 | 96 | 76 | 98 | 110 | 113 | 3 | 3 | 11 | 9 | 20 | 24 | 1 | 3 | 1 | 2 | 0 | 0 |
Unique Cell Count | 182 | 373 | 359 | 278 | 185 | 377 | 446 | 357 | 373 | 416 | 358 | 371 | 145 | 207 | 219 | 189 | 201 | 205 | 181 | 385 | 354 | 206 | 177 | 224 |
Labelled Cell Count | 187 | 378 | 367 | 283 | 199 | 398 | 481 | 372 | 399 | 447 | 390 | 393 | 148 | 208 | 226 | 204 | 223 | 219 | 181 | 385 | 354 | 206 | 177 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.8 | 7.3 | 8.3 | 7.2 | 6.5 | 9.6 | 10.2 | 11.0 | 11.8 | 12.6 | 15.3 | 13.9 | 16.3 | 17.1 | 16.2 | 19.7 | 24.7 | 26.3 | 13.1 | 13.8 | 11.0 |
Std Deviation (1e-4) | 4.8 | 2.7 | 3.7 | 2.3 | 2.3 | 3.4 | 3.5 | 3.7 | 4.6 | 4.4 | 5.5 | 5.4 | 7.1 | 7.2 | 6.7 | 11.2 | 12.8 | 14.2 | 13.7 | 13.6 | 7.7 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.37 | 0.21 | 0.29 | 0.4 | 0.5 | 0.59 | 0.88 | 0.74 | 0.97 | 1.04 | 0.96 | 1.25 | 1.57 | 1.66 | 0.65 | 0.73 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -3.7 | -7.3 | -9.2 | -10.6 | -10.6 | -10.8 | -11.6 | -13.0 | 1.2 | 0.6 | -1.3 | -2.7 | -1.8 | -4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.7 | 3.6 | 2.7 | 6.7 | 3.5 | 4.1 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 3.5 | 3.4 | 3.3 | 4.3 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.3 | 2.6 | 6.9 | 7.9 | 9.1 | 7.4 | 8.3 | 10.0 | 10.0 | 0 | 0 | 1.4 | 1.2 | 3.6 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.225 | 8.9055 | 8.0627 | 6.8564 | 8.4998 | 8.3944 | 10.5129 | 11.9173 | 13.3799 | 10.871 | 11.358 | 12.3935 | 11.0098 | 12.1462 | 11.6288 | 10.8887 | 11.7632 | 11.3402 |
Actin | 0.007 | 0.0014 | 0.0018 | 0.001 | 0.0015 | 0.0008 | 0.0079 | 0.0022 | 0.0187 | 0.0392 | 0.0042 | 0.0007 | 0.0091 | 0.0005 | 0.0212 | 0.015 | 0.0013 | 0.0025 |
Bud | 0.005 | 0.0061 | 0.0023 | 0.001 | 0.0023 | 0.0011 | 0.0009 | 0.0046 | 0.0004 | 0.0017 | 0.0002 | 0.0002 | 0.0041 | 0.0029 | 0.0015 | 0.0004 | 0.0002 | 0.0009 |
Bud Neck | 0.0006 | 0.0005 | 0.0005 | 0.0007 | 0.0006 | 0.0015 | 0.0038 | 0.0006 | 0.0007 | 0.0009 | 0.0008 | 0.001 | 0.0007 | 0.0003 | 0.0005 | 0.0007 | 0.0007 | 0.0016 |
Bud Periphery | 0.0025 | 0.0028 | 0.0009 | 0.0004 | 0.0021 | 0.0004 | 0.0007 | 0.0022 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0031 | 0.0011 | 0.0012 | 0.0003 | 0.0002 | 0.0018 |
Bud Site | 0.004 | 0.0139 | 0.0042 | 0.0007 | 0.0005 | 0.0002 | 0.0208 | 0.0106 | 0.0035 | 0.0011 | 0.0007 | 0.0001 | 0.0009 | 0.0039 | 0.003 | 0.0007 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.9198 | 0.9417 | 0.9411 | 0.9469 | 0.9142 | 0.9295 | 0.8684 | 0.8721 | 0.9392 | 0.829 | 0.7026 | 0.9494 | 0.8843 | 0.9472 | 0.9232 | 0.9292 | 0.9409 | 0.9274 |
Cytoplasmic Foci | 0.0066 | 0.0031 | 0.0059 | 0.0069 | 0.0051 | 0.007 | 0.0168 | 0.0086 | 0.0059 | 0.0236 | 0.0782 | 0.0039 | 0.0122 | 0.0012 | 0.0032 | 0.0087 | 0.0017 | 0.0068 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.002 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0033 | 0.0014 | 0.0029 | 0.0036 | 0.0075 | 0.0026 | 0.0036 | 0.0016 | 0.0018 | 0.0018 | 0.0157 | 0.0019 | 0.0041 | 0.0006 | 0.0027 | 0.0021 | 0.0027 | 0.0017 |
Endosome | 0.0074 | 0.0009 | 0.0024 | 0.0036 | 0.0023 | 0.004 | 0.02 | 0.0038 | 0.0048 | 0.0075 | 0.0876 | 0.001 | 0.0085 | 0.0002 | 0.004 | 0.0043 | 0.01 | 0.0028 |
Golgi | 0.0019 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0081 | 0.0008 | 0.0091 | 0.0193 | 0.0255 | 0.0001 | 0.0032 | 0 | 0.0017 | 0.0048 | 0.0006 | 0.0004 |
Lipid Particles | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0086 | 0.0005 | 0.0003 | 0.002 | 0.0105 | 0 | 0.0058 | 0 | 0.0017 | 0.0038 | 0.0005 | 0.0002 |
Mitochondria | 0.0014 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0024 | 0.008 | 0.0011 | 0.0032 | 0.007 | 0.0042 | 0.0001 | 0.0015 | 0.0001 | 0.0067 | 0.0039 | 0.0026 | 0.0002 |
None | 0.0176 | 0.0105 | 0.0174 | 0.0107 | 0.0381 | 0.0109 | 0.008 | 0.0598 | 0.0021 | 0.0375 | 0.018 | 0.0266 | 0.0365 | 0.0338 | 0.0141 | 0.0044 | 0.0022 | 0.0195 |
Nuclear Periphery | 0.0034 | 0.0023 | 0.003 | 0.004 | 0.0051 | 0.0035 | 0.0077 | 0.0034 | 0.0007 | 0.0016 | 0.0182 | 0.0014 | 0.0034 | 0.0006 | 0.002 | 0.0019 | 0.0184 | 0.0025 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.001 | 0 | 0.0003 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Nucleus | 0.0134 | 0.0123 | 0.0128 | 0.013 | 0.0151 | 0.0294 | 0.0049 | 0.0131 | 0.007 | 0.019 | 0.0103 | 0.0114 | 0.0107 | 0.0066 | 0.008 | 0.0064 | 0.0132 | 0.0294 |
Peroxisomes | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0054 | 0.0011 | 0.0015 | 0.0042 | 0.0061 | 0.0001 | 0.0048 | 0 | 0.0021 | 0.0113 | 0.0001 | 0.0005 |
Punctate Nuclear | 0.001 | 0.001 | 0.0022 | 0.0029 | 0.001 | 0.0043 | 0.0019 | 0.0109 | 0.0004 | 0.002 | 0.013 | 0.0015 | 0.0034 | 0.0003 | 0.0014 | 0.0012 | 0.0014 | 0.0014 |
Vacuole | 0.0026 | 0.001 | 0.0013 | 0.0027 | 0.003 | 0.0011 | 0.0016 | 0.0014 | 0.0003 | 0.0011 | 0.0025 | 0.0004 | 0.0011 | 0.0003 | 0.001 | 0.0007 | 0.0013 | 0.0003 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0007 | 0.0004 | 0.002 | 0.0004 | 0.0001 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0015 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 52.0197 | 26.1986 | 29.1607 | 45.287 | 46.5471 | 46.8651 | 44.3697 | 49.1659 | 73.7263 | 58.9741 |
Translational Efficiency | 1.2217 | 1.6007 | 1.6857 | 1.5692 | 1.421 | 1.6821 | 1.5928 | 1.7836 | 1.7234 | 1.6191 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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