Standard name
Human Ortholog
Description Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.77 0.97 0.98 0.97 0.86 0.83 0.81 0.78 0.66 0.67 0.6 0.95 1.0 1.0 0.97 0.92 0.42 0.57 0.7 0.83 0.87 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.06 0 0 0 0.31 0.33 0.29 0.38 0.52 0.53 0.6 0 0 0 0 0 0.06 0.06 0.11 0.05 0 0.05
Nucleus 0.11 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.19 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.22 0.19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.16 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 6 0 0 3
Bud 0 1 0 1 0 6 10 11 18 18 21 21 0 0 0 0 0 6 3 2 0 1 1
Bud Neck 0 1 10 3 4 2 0 13 9 5 1 6 0 0 0 1 0 3 1 2 0 0 0
Bud Site 0 0 0 0 0 0 1 1 0 3 4 1 0 0 0 0 0
Cell Periphery 1 0 0 2 3 1 4 0 4 1 3 2 0 1 0 0 0 0 0 3 0 0 0
Cytoplasm 271 272 518 385 511 535 665 557 538 465 413 411 19 103 32 219 110 252 235 372 46 85 73
Endoplasmic Reticulum 5 0 1 1 0 1 0 0 0 1 0 1 0 0 0 15 7 2 1 7 0 0 0
Endosome 8 12 2 0 4 2 1 0 1 0 0 1 0 0 0 2 0 8 11 8 0 0 0
Golgi 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 3 5 3 0 0 0 0
Mitochondria 22 21 7 16 18 196 266 202 262 365 324 414 0 0 0 5 5 33 24 59 3 2 4
Nucleus 46 13 3 1 0 2 0 4 2 1 2 0 1 0 0 1 0 113 31 6 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 1 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 3 3 1 2 2 0 0 0 0 0 4 1 0 0 0 0
Peroxisomes 1 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 3 0 0 3 8 9 11 6 15 14 14 22 0 0 0 2 0 30 13 12 0 0 1
Vac/Vac Membrane 90 68 2 1 5 0 8 3 1 2 2 4 0 0 0 5 3 119 65 19 1 2 3
Unique Cell Count 406 354 534 394 529 624 804 689 688 704 616 686 20 103 32 226 120 603 413 529 56 98 91
Labelled Cell Count 448 389 543 413 553 754 972 801 857 876 786 888 20 104 32 250 128 603 413 529 56 98 91


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.2 3.6 3.3 3.2 2.6 2.4 2.8 2.5 2.4 2.4 2.3 4.2 4.9 5.2 4.4 5.0 5.7 5.2 5.2
Std Deviation (1e-4) 0.5 0.9 0.7 1.2 1.2 1.1 1.1 0.9 1.2 0.8 1.0 0.5 0.4 1.5 1.1 1.5 1.6 2.5 1.3 1.0
Intensity Change (Log2) -0.13 -0.18 -0.46 -0.55 -0.34 -0.52 -0.6 -0.55 -0.62 0.25 0.46 0.55 0.3 0.48 0.67 0.53 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3223 0.324 0.2926 0.0003 0.7454 0.3661 2.5964 2.8886 2.6254 2.3788 2.7876 2.8708 2.5355 2.7158 2.5513 3.7129 2.0293 2.6916
Actin 0.0118 0.0005 0.0096 0.0002 0.0096 0.004 0.0314 0.0143 0.004 0.041 0.0032 0.0007 0.02 0.0002 0.0066 0.0041 0.0097 0.0008
Bud 0.0003 0.0038 0.0005 0.0009 0.0004 0.0002 0.0007 0.0024 0.0021 0.0007 0.0025 0.0002 0.0008 0.0023 0.0004 0.0003 0.0002 0.0001
Bud Neck 0.0063 0.0004 0.0008 0.0003 0.0018 0.0039 0.0013 0.0007 0.0079 0.006 0.001 0.0084 0.0041 0.0004 0.0016 0.005 0.0013 0.0021
Bud Periphery 0.0004 0.0007 0.0007 0.0011 0.0007 0.0003 0.0021 0.0071 0.0052 0.0007 0.0107 0.0003 0.0038 0.0027 0.001 0.0004 0.0004 0.0001
Bud Site 0.0021 0.0027 0.0019 0.0004 0.0005 0.0002 0.0009 0.0025 0.0053 0.0154 0.0008 0.0003 0.0054 0.0018 0.0038 0.0027 0.0006 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0002 0.0001 0.0005 0.0004 0.0001 0.0004 0.0001 0.0002 0.0005 0.0001 0
Cytoplasm 0.0693 0.1123 0.0808 0.0568 0.0621 0.1316 0.1271 0.1467 0.1167 0.2034 0.1737 0.1645 0.1917 0.1965 0.1783 0.1878 0.1645 0.2167
Cytoplasmic Foci 0.0071 0.0044 0.0046 0.0044 0.008 0.0103 0.01 0.0049 0.01 0.0125 0.0062 0.0079 0.0179 0.0073 0.0066 0.0086 0.0065 0.0091
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0005 0.0002 0.0002 0.0003 0.0009 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0035 0.0018 0.0027 0.0006 0.0036 0.0025 0.0079 0.0019 0.0036 0.0031 0.0035 0.0014 0.0041 0.0023 0.0026 0.0168 0.0023 0.0018
Endosome 0.007 0.0016 0.0062 0.0014 0.0117 0.0042 0.0077 0.0038 0.0064 0.0078 0.0112 0.0021 0.0063 0.0018 0.0043 0.0095 0.0088 0.0025
Golgi 0.0024 0.0001 0.0005 0.0001 0.0016 0.0006 0.004 0.0019 0.0012 0.0028 0.0014 0.0001 0.0021 0.0001 0.0016 0.0043 0.0032 0.0001
Lipid Particles 0.0017 0.0003 0.0006 0.0003 0.0097 0.0021 0.0031 0.001 0.0041 0.0036 0.0012 0.0003 0.0019 0.0006 0.0018 0.0099 0.0038 0.0006
Mitochondria 0.0018 0.0008 0.0013 0.0009 0.0034 0.0009 0.0024 0.0027 0.0013 0.0012 0.0052 0.0006 0.0014 0.0007 0.0025 0.0036 0.0017 0.0005
None 0.6209 0.6583 0.7047 0.7269 0.5916 0.5739 0.5943 0.6075 0.5918 0.4948 0.6796 0.5877 0.5513 0.6032 0.6506 0.5554 0.5813 0.4945
Nuclear Periphery 0.0205 0.0061 0.0133 0.0046 0.0287 0.0122 0.01 0.0051 0.0141 0.0083 0.0086 0.0063 0.0093 0.0043 0.0062 0.018 0.01 0.0061
Nucleolus 0.0033 0.0036 0.0037 0.005 0.0041 0.0053 0.0025 0.0022 0.0037 0.0013 0.002 0.0027 0.0023 0.0022 0.0024 0.0013 0.0053 0.0027
Nucleus 0.222 0.1657 0.1356 0.1634 0.1412 0.1778 0.1619 0.1687 0.162 0.1761 0.0619 0.1737 0.1273 0.1474 0.1012 0.1428 0.1341 0.216
Peroxisomes 0.0013 0.0002 0.0004 0.0003 0.0624 0.0006 0.0071 0.0009 0.0096 0.0012 0.0009 0.0002 0.0047 0.0002 0.0031 0.0133 0.001 0.0003
Punctate Nuclear 0.0147 0.035 0.03 0.0313 0.0556 0.068 0.0226 0.0214 0.0485 0.0166 0.0114 0.0414 0.0422 0.0246 0.0231 0.0121 0.0633 0.0446
Vacuole 0.0019 0.0013 0.0013 0.0009 0.0019 0.001 0.0016 0.0036 0.0017 0.0022 0.0101 0.001 0.0023 0.0012 0.0017 0.0026 0.001 0.0009
Vacuole Periphery 0.0012 0.0002 0.0004 0.0002 0.0012 0.0004 0.0006 0.0004 0.0005 0.0005 0.0038 0.0002 0.0005 0.0002 0.0003 0.0006 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.7031 50.0336 47.2526 59.0999 55.1323 69.7419 80.8108 70.7271 65.5051 72.4972
Translational Efficiency 1.2802 1.4937 1.354 1.0511 1.0987 1.0785 0.9673 1.22 1.1189 1.0783

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (15%), nucleus (8%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vhr2

Vhr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vhr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available