Standard name | |
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Human Ortholog | |
Description | Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 3 | 6 | 0 | 0 | 3 |
Bud | 0 | 1 | 0 | 1 | 0 | 6 | 10 | 11 | 18 | 18 | 21 | 21 | 0 | 0 | 0 | – | 0 | 0 | 6 | 3 | 2 | 0 | 1 | 1 |
Bud Neck | 0 | 1 | 10 | 3 | 4 | 2 | 0 | 13 | 9 | 5 | 1 | 6 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 2 | 3 | 1 | 4 | 0 | 4 | 1 | 3 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 271 | 272 | 518 | 385 | 511 | 535 | 665 | 557 | 538 | 465 | 413 | 411 | 19 | 103 | 32 | – | 219 | 110 | 252 | 235 | 372 | 46 | 85 | 73 |
Endoplasmic Reticulum | 5 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 15 | 7 | 2 | 1 | 7 | 0 | 0 | 0 |
Endosome | 8 | 12 | 2 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 8 | 11 | 8 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 5 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 22 | 21 | 7 | 16 | 18 | 196 | 266 | 202 | 262 | 365 | 324 | 414 | 0 | 0 | 0 | – | 5 | 5 | 33 | 24 | 59 | 3 | 2 | 4 |
Nucleus | 46 | 13 | 3 | 1 | 0 | 2 | 0 | 4 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | – | 1 | 0 | 113 | 31 | 6 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 1 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 0 | 3 | 8 | 9 | 11 | 6 | 15 | 14 | 14 | 22 | 0 | 0 | 0 | – | 2 | 0 | 30 | 13 | 12 | 0 | 0 | 1 |
Vac/Vac Membrane | 90 | 68 | 2 | 1 | 5 | 0 | 8 | 3 | 1 | 2 | 2 | 4 | 0 | 0 | 0 | – | 5 | 3 | 119 | 65 | 19 | 1 | 2 | 3 |
Unique Cell Count | 406 | 354 | 534 | 394 | 529 | 624 | 804 | 689 | 688 | 704 | 616 | 686 | 20 | 103 | 32 | 226 | 120 | 603 | 413 | 529 | 56 | 98 | 91 | |
Labelled Cell Count | 448 | 389 | 543 | 413 | 553 | 754 | 972 | 801 | 857 | 876 | 786 | 888 | 20 | 104 | 32 | 250 | 128 | 603 | 413 | 529 | 56 | 98 | 91 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.2 | 3.6 | 3.3 | 3.2 | 2.6 | 2.4 | 2.8 | 2.5 | 2.4 | 2.4 | 2.3 | 4.2 | 4.9 | 5.2 | 4.4 | 5.0 | 5.7 | – | 5.2 | 5.2 |
Std Deviation (1e-4) | 0.5 | 0.9 | 0.7 | 1.2 | 1.2 | 1.1 | 1.1 | 0.9 | 1.2 | 0.8 | 1.0 | 0.5 | 0.4 | 1.5 | 1.1 | 1.5 | 1.6 | 2.5 | – | 1.3 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.18 | -0.46 | -0.55 | -0.34 | -0.52 | -0.6 | -0.55 | -0.62 | 0.25 | 0.46 | 0.55 | 0.3 | 0.48 | 0.67 | – | 0.53 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3223 | 0.324 | 0.2926 | 0.0003 | 0.7454 | 0.3661 | 2.5964 | 2.8886 | 2.6254 | 2.3788 | 2.7876 | 2.8708 | 2.5355 | 2.7158 | 2.5513 | 3.7129 | 2.0293 | 2.6916 |
Actin | 0.0118 | 0.0005 | 0.0096 | 0.0002 | 0.0096 | 0.004 | 0.0314 | 0.0143 | 0.004 | 0.041 | 0.0032 | 0.0007 | 0.02 | 0.0002 | 0.0066 | 0.0041 | 0.0097 | 0.0008 |
Bud | 0.0003 | 0.0038 | 0.0005 | 0.0009 | 0.0004 | 0.0002 | 0.0007 | 0.0024 | 0.0021 | 0.0007 | 0.0025 | 0.0002 | 0.0008 | 0.0023 | 0.0004 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0063 | 0.0004 | 0.0008 | 0.0003 | 0.0018 | 0.0039 | 0.0013 | 0.0007 | 0.0079 | 0.006 | 0.001 | 0.0084 | 0.0041 | 0.0004 | 0.0016 | 0.005 | 0.0013 | 0.0021 |
Bud Periphery | 0.0004 | 0.0007 | 0.0007 | 0.0011 | 0.0007 | 0.0003 | 0.0021 | 0.0071 | 0.0052 | 0.0007 | 0.0107 | 0.0003 | 0.0038 | 0.0027 | 0.001 | 0.0004 | 0.0004 | 0.0001 |
Bud Site | 0.0021 | 0.0027 | 0.0019 | 0.0004 | 0.0005 | 0.0002 | 0.0009 | 0.0025 | 0.0053 | 0.0154 | 0.0008 | 0.0003 | 0.0054 | 0.0018 | 0.0038 | 0.0027 | 0.0006 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 |
Cytoplasm | 0.0693 | 0.1123 | 0.0808 | 0.0568 | 0.0621 | 0.1316 | 0.1271 | 0.1467 | 0.1167 | 0.2034 | 0.1737 | 0.1645 | 0.1917 | 0.1965 | 0.1783 | 0.1878 | 0.1645 | 0.2167 |
Cytoplasmic Foci | 0.0071 | 0.0044 | 0.0046 | 0.0044 | 0.008 | 0.0103 | 0.01 | 0.0049 | 0.01 | 0.0125 | 0.0062 | 0.0079 | 0.0179 | 0.0073 | 0.0066 | 0.0086 | 0.0065 | 0.0091 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.0018 | 0.0027 | 0.0006 | 0.0036 | 0.0025 | 0.0079 | 0.0019 | 0.0036 | 0.0031 | 0.0035 | 0.0014 | 0.0041 | 0.0023 | 0.0026 | 0.0168 | 0.0023 | 0.0018 |
Endosome | 0.007 | 0.0016 | 0.0062 | 0.0014 | 0.0117 | 0.0042 | 0.0077 | 0.0038 | 0.0064 | 0.0078 | 0.0112 | 0.0021 | 0.0063 | 0.0018 | 0.0043 | 0.0095 | 0.0088 | 0.0025 |
Golgi | 0.0024 | 0.0001 | 0.0005 | 0.0001 | 0.0016 | 0.0006 | 0.004 | 0.0019 | 0.0012 | 0.0028 | 0.0014 | 0.0001 | 0.0021 | 0.0001 | 0.0016 | 0.0043 | 0.0032 | 0.0001 |
Lipid Particles | 0.0017 | 0.0003 | 0.0006 | 0.0003 | 0.0097 | 0.0021 | 0.0031 | 0.001 | 0.0041 | 0.0036 | 0.0012 | 0.0003 | 0.0019 | 0.0006 | 0.0018 | 0.0099 | 0.0038 | 0.0006 |
Mitochondria | 0.0018 | 0.0008 | 0.0013 | 0.0009 | 0.0034 | 0.0009 | 0.0024 | 0.0027 | 0.0013 | 0.0012 | 0.0052 | 0.0006 | 0.0014 | 0.0007 | 0.0025 | 0.0036 | 0.0017 | 0.0005 |
None | 0.6209 | 0.6583 | 0.7047 | 0.7269 | 0.5916 | 0.5739 | 0.5943 | 0.6075 | 0.5918 | 0.4948 | 0.6796 | 0.5877 | 0.5513 | 0.6032 | 0.6506 | 0.5554 | 0.5813 | 0.4945 |
Nuclear Periphery | 0.0205 | 0.0061 | 0.0133 | 0.0046 | 0.0287 | 0.0122 | 0.01 | 0.0051 | 0.0141 | 0.0083 | 0.0086 | 0.0063 | 0.0093 | 0.0043 | 0.0062 | 0.018 | 0.01 | 0.0061 |
Nucleolus | 0.0033 | 0.0036 | 0.0037 | 0.005 | 0.0041 | 0.0053 | 0.0025 | 0.0022 | 0.0037 | 0.0013 | 0.002 | 0.0027 | 0.0023 | 0.0022 | 0.0024 | 0.0013 | 0.0053 | 0.0027 |
Nucleus | 0.222 | 0.1657 | 0.1356 | 0.1634 | 0.1412 | 0.1778 | 0.1619 | 0.1687 | 0.162 | 0.1761 | 0.0619 | 0.1737 | 0.1273 | 0.1474 | 0.1012 | 0.1428 | 0.1341 | 0.216 |
Peroxisomes | 0.0013 | 0.0002 | 0.0004 | 0.0003 | 0.0624 | 0.0006 | 0.0071 | 0.0009 | 0.0096 | 0.0012 | 0.0009 | 0.0002 | 0.0047 | 0.0002 | 0.0031 | 0.0133 | 0.001 | 0.0003 |
Punctate Nuclear | 0.0147 | 0.035 | 0.03 | 0.0313 | 0.0556 | 0.068 | 0.0226 | 0.0214 | 0.0485 | 0.0166 | 0.0114 | 0.0414 | 0.0422 | 0.0246 | 0.0231 | 0.0121 | 0.0633 | 0.0446 |
Vacuole | 0.0019 | 0.0013 | 0.0013 | 0.0009 | 0.0019 | 0.001 | 0.0016 | 0.0036 | 0.0017 | 0.0022 | 0.0101 | 0.001 | 0.0023 | 0.0012 | 0.0017 | 0.0026 | 0.001 | 0.0009 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0004 | 0.0002 | 0.0012 | 0.0004 | 0.0006 | 0.0004 | 0.0005 | 0.0005 | 0.0038 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0006 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.7031 | 50.0336 | 47.2526 | 59.0999 | 55.1323 | 69.7419 | 80.8108 | 70.7271 | 65.5051 | 72.4972 |
Translational Efficiency | 1.2802 | 1.4937 | 1.354 | 1.0511 | 1.0987 | 1.0785 | 0.9673 | 1.22 | 1.1189 | 1.0783 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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