Standard name
Human Ortholog
Description Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.1 0.14 0 0 0 0.05 0.06 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.2 0.13 0.17 0 0 0 0 0 0 0 0 0 0 0.65 0.74 0.57 0.09 0.1 0.09 0.1 0.06 0.06
Mitochondria 0.9 0.9 0.86 0.92 0.82 0.8 0.82 0.8 0.91 0.89 0.9 0.87 0.95 0.92 0.91 0.12 0.09 0.12 0.73 0.71 0.77 0.7 0.63 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0 0 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.12 0 0 0.09 0.12 0.1 0.11 0.14 0.08 0.12 0.1 0.1 0.06 0.07 0.1 0.09 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.12
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.1 0 0.06 0.08 0 0.07 0 0 0 0.05 0.05 0.06 0 0.16 0.14 0.16 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 9 1 5 1 4 3 0 2 2 0 6 1 6 28 19 27 3 6 5 10 23 22
Bud 0 3 0 4 1 2 13 8 7 3 8 3 3 3 5 1 1 1 0 2 1 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud Site 0 2 0 0 0 1 1 0 5 2 0 1 0 0 0 1 0 1 – – – – – –
Cell Periphery 0 1 8 2 3 1 3 0 2 0 0 2 3 3 5 9 8 2 0 0 0 0 1 2
Cytoplasm 4 0 5 6 9 14 12 10 9 8 7 5 2 6 9 10 3 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 2 1 3 2 0 0 0 1 0 0 2 5 1 5 0 0 1 0 1 2
Endosome 3 0 2 2 3 3 6 6 0 0 0 1 0 1 0 0 1 5 3 1 3 1 9 6
Golgi 0 4 39 25 40 4 9 10 0 0 0 1 7 5 6 135 139 110 11 14 20 18 23 20
Mitochondria 112 114 165 172 192 192 298 253 243 232 163 181 350 331 399 25 16 24 92 97 168 134 242 239
Nucleus 1 1 0 0 0 2 4 1 1 4 0 1 1 0 1 0 2 3 0 0 0 0 0 0
Nuclear Periphery 5 2 0 1 6 1 23 13 11 6 14 13 1 1 3 1 0 1 1 0 0 0 4 3
Nucleolus 24 15 5 4 20 29 37 34 38 21 22 21 38 23 31 20 16 18 3 2 0 1 5 6
Peroxisomes 0 2 8 0 8 3 12 7 4 3 3 6 4 12 16 3 1 5 4 4 8 19 51 41
SpindlePole 1 0 1 0 1 2 6 6 3 1 2 3 0 1 0 0 0 2 1 0 0 1 3 1
Vac/Vac Membrane 4 8 19 8 14 20 16 21 5 10 3 11 20 20 18 33 27 32 2 6 6 2 8 6
Unique Cell Count 125 126 192 187 235 240 363 317 266 260 181 208 368 361 438 208 188 194 127 137 220 193 386 363
Labelled Cell Count 154 152 261 225 304 276 447 374 328 292 224 250 435 407 501 271 234 242 127 137 220 193 386 363


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.9 18.6 19.6 21.9 21.4 17.4 16.4 14.9 12.9 11.8 11.2 12.6 26.6 25.4 24.3 34.4 34.5 33.0 20.0 20.6 19.5
Std Deviation (1e-4) 6.1 5.0 5.3 6.1 6.3 6.1 5.3 4.6 4.1 3.5 3.6 4.3 6.8 6.5 6.6 12.0 10.0 11.8 5.0 5.4 5.2
Intensity Change (Log2) – – – 0.16 0.13 -0.17 -0.26 -0.4 -0.61 -0.74 -0.81 -0.64 0.44 0.37 0.31 0.81 0.81 0.75 0.03 0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.5 0 0 0 0 0 -2.0 0 -2.1 0 0 3.0 2.0 3.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0
Cytoplasm 0.4 0.7 1.6 0.5 0.4 0.5 0.3 0.7 0 0 0 0 1.2 0 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.8 -0.9 -6.4 -7.1 -6.4 -7.7 -7.6 -6.4 -6.6 -7.5 -7.8 -8.5 9.0 10.5 7.3
Mitochondria 1.9 -1.2 -1.6 -1.2 -1.7 1.8 1.1 1.2 0.3 3.8 2.1 1.9 -14.8 -15.1 -14.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3.6 0 0 0 3.9 3.5 0 0 0 0 0 0
Nucleolus 0 2.6 3.6 3.2 3.3 4.2 2.5 3.6 3.0 3.3 1.9 2.2 2.9 2.5 2.8
Peroxisomes 0 -0.4 0 -0.5 -1.3 -1.8 0 -1.4 -0.7 0 -0.5 -0.3 -1.7 0 -0.9
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 -1.5 -0.6 -2.5 -1.3 -3.8 -2.6 -3.4 -1.7 -2.0 -1.9 -2.8 1.8 1.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 58.788 59.0815 60.0047 57.0128 61.2051 57.5323 66.8718 70.4886 67.9357 60.1286 58.4279 66.3399 60.121 61.4533 60.6676 47.9478 56.4661 62.5203
Actin 0.0086 0.001 0.0028 0.0202 0 0.0001 0.0158 0.0009 0.0011 0.0161 0.0003 0 0.0004 0.0009 0.0011 0 0 0.0001
Bud 0 0.0002 0.0001 0.0001 0 0 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0 0 0 0
Bud Neck 0.0004 0 0.0001 0.0009 0 0 0.0002 0 0 0 0 0 0 0.0001 0 0 0 0
Bud Periphery 0.0001 0.0005 0.0002 0.0001 0 0 0.0003 0 0.0001 0.0005 0 0 0.0001 0 0 0 0 0
Bud Site 0.0002 0.0005 0.0009 0.0004 0 0 0.0003 0 0.0003 0.0002 0 0 0.0004 0.0005 0 0 0 0
Cell Periphery 0.0002 0.0003 0.0002 0.0002 0 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0004 0 0 0.0044 0 0 0.0007 0 0.0005 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasmic Foci 0.0052 0 0.0003 0.0033 0 0.001 0.0119 0.0001 0.0079 0.0027 0.001 0 0.0061 0.0028 0.0048 0.0001 0.0022 0
Eisosomes 0.0006 0.0003 0.0008 0.0015 0.0001 0.0002 0.002 0.0025 0.0014 0.0004 0.0008 0.0002 0.0006 0.0003 0.0003 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0006 0 0 0.0042 0 0 0.001 0 0.0001 0.0002 0 0 0 0 0.0001 0 0 0
Endosome 0.003 0.0001 0.0001 0.0062 0 0 0.0061 0.0001 0.0021 0.0109 0 0 0.0002 0.0006 0.0008 0.0001 0.0001 0
Golgi 0.0054 0.0002 0.0003 0.0026 0.0001 0.0001 0.0052 0.0004 0.0006 0.0074 0 0.0001 0.0002 0.0014 0.0003 0.0002 0.0001 0.0001
Lipid Particles 0.0096 0.0067 0.0043 0.0047 0.0026 0.0047 0.0098 0.0034 0.0053 0.0079 0.0048 0.001 0.0057 0.0058 0.0033 0.0032 0.0036 0.0015
Mitochondria 0.9391 0.9628 0.9786 0.9268 0.9929 0.9435 0.9252 0.9847 0.9545 0.9358 0.9768 0.9968 0.9469 0.9686 0.9686 0.9879 0.9758 0.9897
None 0.0017 0 0 0.0094 0 0.0001 0.001 0 0.0008 0.0003 0.0001 0 0.0006 0 0.0002 0 0 0
Nuclear Periphery 0.0008 0.0001 0 0.004 0 0.0001 0.0026 0 0.0006 0.0004 0 0 0.0001 0 0.0012 0 0 0
Nucleolus 0.0068 0.0002 0.0003 0.0005 0.0001 0.0107 0.0021 0.0002 0.0062 0.0001 0.0089 0 0.0221 0.0007 0.004 0.0028 0.0141 0.0006
Nucleus 0.0004 0.0001 0 0.0011 0 0.0003 0.0005 0 0.0011 0.0001 0.0003 0 0.0016 0.0001 0.0004 0.0003 0.0006 0
Peroxisomes 0.0078 0.001 0.005 0.0017 0.0002 0.0006 0.0055 0.0036 0.0025 0.0101 0.0017 0.0002 0.0021 0.0025 0.0009 0.0004 0.0006 0.0002
Punctate Nuclear 0.0012 0 0 0.0062 0 0 0.0006 0 0.0006 0.0009 0 0 0.0006 0 0.0003 0 0.0001 0
Vacuole 0.0008 0.0012 0.0004 0.0002 0.0003 0.0007 0.0012 0.0003 0.0018 0.0019 0.0009 0 0.0017 0.001 0.0012 0.0006 0.0006 0.0002
Vacuole Periphery 0.0069 0.0249 0.0054 0.0012 0.0035 0.0378 0.0077 0.0036 0.0122 0.0038 0.0038 0.0016 0.0098 0.0141 0.0119 0.0042 0.0019 0.0071

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.0394 26.2577 23.1553 37.8052 42.4989 86.3685 92.7296 88.5556 85.3338 94.0818
Translational Efficiency 0.9257 0.9488 0.9911 0.7279 0.7941 0.9985 0.8717 0.7417 0.79 0.791

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Arg5,6

Arg5,6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg5,6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available