Standard name
Human Ortholog
Description Subunit of a complex that associates with actin filaments; forms a complex with Aim21p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; TcTex1-type dynein light chain family member; null mutant is sensitive to expression of the top1-T722A allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.05 0.11 0 0.06 0 – 0.05 0 0.05 0.05 0 0.05 0.05 0 0 0 0 0.13 0.1 0.17 0.12 0.12 0.08
Bud 0 0 0.07 0.05 0.08 0.05 – 0.05 0.05 0.1 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.93 0.8 0.84 0.71 0.71 – 0.59 0.48 0.48 0.45 0.42 0.91 0.92 0.91 0.95 0.98 0.9 0.77 0.79 0.56 0.65 0.75 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.15 0.15 0.37 – 0.46 0.6 0.57 0.61 0.66 0 0 0 0 0 0.05 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0.05 0.05 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 21 7 6 6 22 8 – 29 17 27 26 22 12 17 10 6 1 1 36 15 14 5 26 24
Bud 10 5 4 12 31 19 – 32 25 60 35 52 5 7 9 5 1 1 2 3 1 1 4 10
Bud Neck 0 0 2 0 2 0 – 1 0 1 0 2 0 0 0 1 1 2 3 1 4 0 5 7
Bud Site 1 0 1 2 10 9 – 14 16 8 14 27 2 0 0 0 0 1 – – – – – –
Cell Periphery 0 1 2 3 1 2 – 7 4 8 10 10 1 2 8 8 3 2 0 0 0 0 0 1
Cytoplasm 229 137 43 211 268 292 – 354 250 287 213 267 219 314 305 159 123 126 217 123 47 29 165 225
Endoplasmic Reticulum 5 3 1 4 7 8 – 6 12 4 14 8 7 3 1 16 8 12 2 0 0 0 0 0
Endosome 0 1 0 2 15 2 – 3 3 4 1 3 0 1 3 1 0 4 9 3 3 1 6 5
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 0 0
Mitochondria 11 1 9 38 55 153 – 273 317 340 290 419 3 0 3 3 1 7 3 4 8 1 1 7
Nucleus 0 0 0 0 1 1 – 1 4 3 3 2 0 2 0 0 0 0 0 0 0 0 1 2
Nuclear Periphery 0 0 0 0 0 0 – 4 5 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 5 2 4 3 3 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 6 17 13 – 19 25 29 12 36 0 7 18 0 0 0 1 0 1 0 1 1
SpindlePole 0 0 1 7 27 3 – 21 9 18 7 20 1 2 2 1 0 1 1 1 0 1 3 2
Vac/Vac Membrane 1 0 0 0 5 6 – 14 11 14 3 7 1 0 1 0 0 2 0 1 0 2 2 2
Unique Cell Count 265 147 54 252 375 411 598 525 598 475 639 240 340 336 168 126 140 284 156 85 46 222 296
Labelled Cell Count 278 155 69 291 462 516 783 700 807 633 878 251 355 360 200 139 164 284 156 85 46 222 296


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.2 3.3 4.0 4.3 3.7 – 3.8 3.3 3.5 3.5 3.4 5.3 5.7 5.6 5.7 6.0 6.2 4.7 5.2 5.5
Std Deviation (1e-4) 0.7 1.0 0.6 1.4 1.3 1.3 – 1.1 1.0 1.0 1.0 1.0 1.1 1.3 1.4 1.6 2.0 2.5 0.7 1.3 1.4
Intensity Change (Log2) – – – 0.28 0.39 0.18 – 0.21 0.01 0.11 0.09 0.04 0.7 0.8 0.77 0.79 0.87 0.92 0.52 0.66 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -1.3 -1.3 – -2.9 -4.5 -4.5 -4.8 -5.4 2.5 3.0 2.5 3.4 4.1 1.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6972 1.1422 1.163 0.6589 1.2169 0.8999 0.6164 0.6466 0.5943 0.3431 0.4699 0.6104 2.2844 2.6817 2.6801 2.6429 2.5098 2.5605
Actin 0.4828 0.2987 0.4337 0.4815 0.3976 0.4533 0.4306 0.3984 0.552 0.4622 0.4717 0.4664 0.4604 0.3764 0.5308 0.4703 0.5311 0.4721
Bud 0.0015 0.0032 0.0137 0.0276 0.0012 0.002 0.0017 0.0048 0.0195 0.027 0.0035 0.0022 0.0013 0.0049 0.0143 0.0171 0.0061 0.0007
Bud Neck 0.0189 0.0146 0.0161 0.0052 0.001 0.2302 0.0093 0.0046 0.0211 0.0024 0.1248 0.1535 0.0239 0.0074 0.0186 0.0048 0.0026 0.2202
Bud Periphery 0.0022 0.0066 0.0264 0.0743 0.0018 0.0053 0.0033 0.0061 0.0368 0.0659 0.0049 0.0043 0.0021 0.0064 0.0335 0.061 0.0148 0.0009
Bud Site 0.041 0.1962 0.0676 0.0095 0.0058 0.0024 0.0233 0.1305 0.0386 0.0046 0.0041 0.0045 0.0796 0.1851 0.0504 0.0055 0.003 0.0016
Cell Periphery 0.002 0.0038 0.002 0.0006 0.0002 0.0006 0.0008 0.0015 0.0015 0.0011 0.0005 0.0006 0.0046 0.0046 0.0017 0.0023 0.0004 0.0005
Cytoplasm 0.1073 0.2303 0.1372 0.0055 0.0878 0.106 0.0808 0.257 0.1181 0.1524 0.0284 0.0836 0.1128 0.2349 0.1467 0.1037 0.0657 0.1024
Cytoplasmic Foci 0.1374 0.0661 0.086 0.1591 0.1312 0.0782 0.154 0.0467 0.0524 0.0808 0.153 0.1105 0.0936 0.0676 0.0358 0.0833 0.0903 0.0818
Eisosomes 0.0017 0.0013 0.0012 0.0008 0.0006 0.0005 0.0008 0.0006 0.001 0.0004 0.0005 0.0006 0.0019 0.0016 0.0007 0.0014 0.0007 0.0005
Endoplasmic Reticulum 0.0017 0.0019 0.0031 0.0002 0.0011 0.003 0.0037 0.0022 0.0024 0.0049 0.001 0.0047 0.0035 0.0017 0.003 0.0019 0.0017 0.001
Endosome 0.0189 0.0036 0.0175 0.0272 0.0057 0.0072 0.0514 0.008 0.0164 0.0318 0.0412 0.0232 0.038 0.0054 0.0087 0.016 0.0191 0.009
Golgi 0.0213 0.0035 0.0104 0.0194 0.0093 0.0102 0.0273 0.0104 0.0175 0.0167 0.0516 0.0226 0.0182 0.0097 0.0108 0.0253 0.0284 0.0083
Lipid Particles 0.0113 0.004 0.0131 0.0382 0.0104 0.0086 0.0284 0.0009 0.0029 0.0019 0.029 0.0105 0.0131 0.0014 0.005 0.0167 0.0244 0.0033
Mitochondria 0.0039 0.001 0.0126 0.0054 0.0711 0.006 0.012 0.003 0.0166 0.0081 0.0069 0.0091 0.0056 0.001 0.0107 0.0176 0.0146 0.0017
None 0.0827 0.1442 0.1177 0.0063 0.151 0.0607 0.0541 0.0814 0.0617 0.1076 0.0084 0.0516 0.0575 0.0649 0.0696 0.0324 0.0801 0.0491
Nuclear Periphery 0.0019 0.0003 0.0013 0.0001 0.0004 0.0029 0.0041 0.0004 0.0004 0.0011 0.0006 0.0047 0.0092 0.0003 0.0006 0.0007 0.001 0.0015
Nucleolus 0.0011 0.0002 0.0002 0.0005 0.0002 0.0003 0.0025 0.0002 0.0002 0.0001 0.0001 0.0003 0.0021 0.0001 0.0002 0.0002 0.0002 0.0001
Nucleus 0.0014 0.0005 0.0008 0.0001 0.0003 0.001 0.0049 0.0006 0.0005 0.0008 0.0002 0.0011 0.0028 0.0005 0.0006 0.0005 0.0003 0.0003
Peroxisomes 0.0547 0.0179 0.0347 0.1337 0.1205 0.0179 0.0923 0.0412 0.0381 0.0245 0.0668 0.0407 0.0583 0.0244 0.0555 0.1334 0.1124 0.0425
Punctate Nuclear 0.005 0.001 0.0031 0.0036 0.0018 0.0019 0.0103 0.0009 0.0006 0.0012 0.0017 0.003 0.0057 0.0008 0.0008 0.0047 0.0014 0.0021
Vacuole 0.0009 0.0008 0.0009 0.001 0.0004 0.0006 0.0027 0.0007 0.0011 0.0037 0.0005 0.0012 0.0042 0.0006 0.0014 0.0007 0.001 0.0002
Vacuole Periphery 0.0004 0.0001 0.0004 0.0002 0.0004 0.0009 0.0015 0.0001 0.0004 0.001 0.0005 0.0011 0.0012 0.0001 0.0006 0.0005 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.5127 2.5889 7.6843 21.0751 28.1617 11.5779 16.3603 18.5507 14.2944 21.4018
Translational Efficiency 0.5235 9.9531 3.2673 1.3271 0.7496 2.2681 1.8581 1.8168 2.2811 1.5776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a complex that associates with actin filaments; forms a complex with Aim21p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; TcTex1-type dynein light chain family member; null mutant is sensitive to expression of the top1-T722A allele
Localization
Cell Percentages actin (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tda2

Tda2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available