Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.05 0 0 0 0.08 0.06 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.94 0.92 0.8 0.8 0.66 0.63 0.63 0.6 0.53 0.57 1.0 0.95 0.93 0.95 0.93 0.9 0.93 0.91 0.88 0.89 0.9 0.92
Endoplasmic Reticulum 0 0 0 0.06 0.09 0.11 0.11 0.14 0.11 0.1 0.1 0.11 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0.05 0.13 0.05 0.07 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.2 0.2 0.17 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 0.06 0.07 0.07 0.15 0.07 0 0 0 0 0 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 8 6 5 12 20 12 12 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 3 2 2 2 0 1 0 0 0 0 1 0 0 2 3 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 3 3 0 7 7 9 16 13 12 10 15 0 1 1 1 0 0 0 0 2 0 0 0
Cytoplasm 236 307 194 142 125 152 129 175 153 128 82 103 122 144 115 115 57 90 234 303 202 120 167 145
Endoplasmic Reticulum 1 8 7 10 14 20 21 39 27 22 15 19 0 3 3 2 4 3 4 5 8 1 7 6
Endosome 0 0 0 0 0 0 2 1 2 0 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 1 0 1 8 8 10 37 13 14 7 14 0 4 4 4 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2 1 9 8 49 42 27 39 0 1 1 3 2 0 0 0 1 0 0 0
Nucleus 0 1 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 1 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 1 3 4 2 5 4 25 18 16 15 24 13 0 1 0 3 2 11 3 7 3 3 3 1
Unique Cell Count 238 319 206 154 157 189 194 279 242 214 155 180 122 152 123 121 61 100 253 332 231 135 186 158
Labelled Cell Count 238 324 209 156 170 200 215 310 296 248 179 222 122 156 125 128 68 104 253 332 231 135 186 158


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 86.0 66.7 66.4 58.3 34.5 31.1 22.4 24.8 19.9 19.7 18.6 17.6 75.4 65.4 53.3 66.1 82.5 71.0 77.8 86.3 84.5
Std Deviation (1e-4) 19.7 18.5 21.9 15.1 12.5 9.6 8.3 8.4 7.8 6.3 6.5 6.6 22.0 18.6 17.4 25.1 27.0 26.3 22.3 24.5 23.8
Intensity Change (Log2) – – – -0.19 -0.94 -1.09 -1.57 -1.42 -1.74 -1.76 -1.84 -1.92 0.18 -0.02 -0.32 -0.01 0.31 0.1 0.23 0.38 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.0 4.2 3.4 4.1 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.9 2.4 2.2 2.3 2.5 3.2 0 0 0 0 0 0
Cytoplasm -0.7 -4.2 -4.1 -7.0 -8.0 -7.8 -8.3 -9.1 -8.6 2.7 0.2 -0.2 0.3 -0.2 -1.3
Endoplasmic Reticulum 1.4 2.2 2.8 2.9 3.9 3.1 2.8 2.5 2.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 5.4 3.4 3.7 0 4.1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.8 6.7 6.2 7.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.2 2.4 2.4 2.5 4.8 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0162 0.0018 0.0116 0.0015 0.005 0.0095 – – – – – – 0.0066 0.0003 0.0035 0.0012 0.0053 0.0032
Bud 0.0033 0.002 0.0047 0.0021 0.0037 0.005 – – – – – – 0.0027 0.0006 0.0016 0.0013 0.0016 0.0064
Bud Neck 0.002 0.0008 0.0029 0.0009 0.0017 0.0048 – – – – – – 0.0011 0.0005 0.001 0.0007 0.0009 0.0018
Bud Periphery 0.0048 0.0021 0.0041 0.0014 0.0055 0.0022 – – – – – – 0.0031 0.0004 0.0015 0.0009 0.001 0.0031
Bud Site 0.0016 0.0016 0.006 0.0008 0.0018 0.0038 – – – – – – 0.0029 0.0005 0.0012 0.0008 0.0009 0.0007
Cell Periphery 0.0438 0.0279 0.0355 0.0537 0.0252 0.0034 – – – – – – 0.0362 0.0111 0.02 0.0108 0.0141 0.0049
Cytoplasm 0.4457 0.8039 0.5936 0.5274 0.4811 0.655 – – – – – – 0.5945 0.9073 0.7541 0.577 0.6529 0.6684
Cytoplasmic Foci 0.0038 0.0013 0.0094 0.0026 0.0031 0.0293 – – – – – – 0.0189 0.0011 0.0063 0.0243 0.0087 0.0388
Eisosomes 0.0003 0.0002 0.0005 0.0001 0.0001 0.0003 – – – – – – 0.001 0 0.0002 0.0009 0.0002 0.0003
Endoplasmic Reticulum 0.0348 0.0063 0.0099 0.0104 0.0085 0.0468 – – – – – – 0.0477 0.021 0.0114 0.0049 0.0137 0.0442
Endosome 0.0161 0.0036 0.0091 0.0136 0.0236 0.0311 – – – – – – 0.0093 0.002 0.0125 0.0808 0.0495 0.0243
Golgi 0.0036 0.001 0.0034 0.0007 0.0063 0.0245 – – – – – – 0.0032 0.0004 0.0046 0.0053 0.0111 0.0119
Lipid Particles 0.0038 0.001 0.0032 0.0032 0.0025 0.0043 – – – – – – 0.0089 0.0008 0.0054 0.0083 0.004 0.0066
Mitochondria 0.0224 0.025 0.0268 0.0014 0.1155 0.0254 – – – – – – 0.014 0.0007 0.0177 0.0235 0.0143 0.0318
None 0.003 0.0004 0.0023 0.0015 0.0011 0.0075 – – – – – – 0.0112 0.0002 0.0051 0.0211 0.0035 0.0179
Nuclear Periphery 0.0447 0.0047 0.0096 0.0174 0.0173 0.0091 – – – – – – 0.0189 0.0028 0.0079 0.0047 0.0278 0.0042
Nucleolus 0.0027 0.0006 0.0051 0.0011 0.0251 0.0121 – – – – – – 0.0122 0.0002 0.0027 0.0022 0.0028 0.0167
Nucleus 0.0228 0.004 0.0096 0.0079 0.0187 0.0176 – – – – – – 0.0193 0.0018 0.0072 0.0037 0.012 0.0058
Peroxisomes 0.0005 0.0001 0.0008 0.0001 0.0007 0.0015 – – – – – – 0.0016 0 0.0009 0.0028 0.0005 0.0011
Punctate Nuclear 0.0012 0 0.0003 0.0002 0.0002 0.0112 – – – – – – 0.004 0 0.0019 0.0022 0.0135 0.0015
Vacuole 0.2459 0.0981 0.2106 0.2801 0.2125 0.0657 – – – – – – 0.1506 0.045 0.114 0.1778 0.1226 0.0728
Vacuole Periphery 0.0769 0.0135 0.0411 0.0718 0.0407 0.0298 – – – – – – 0.0323 0.0033 0.0193 0.0448 0.039 0.0338

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2950.5251 3021.9858 3756.542 2541.3291 3452.053 3005.6531 2561.1425 2258.6032 2919.873 2135.2856
Translational Efficiency 1.1356 1.2355 1.0385 1.6345 1.2533 1.1668 1.2217 1.4433 1.0535 1.4758

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps24a

Rps24a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps24a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available