Standard name
Human Ortholog
Description 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0.05 0.13 0.08 0.06 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.98 1.0 0.96 0.87 0.85 0.85 0.83 0.8 0.72 0.98 0.97 0.99 0.97 0.96 0.98 0.98 0.95 0.88 0.95 0.95 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.08 0.06 0.11 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 3 3 0 6 5 0 0 0 1 0 0 0 1 1 0 0 2
Bud 0 1 0 0 0 2 2 0 1 0 0 1 0 0 0 1 0 0 0 2 0 0 0 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 2 0 0 7 6 15 5 4 22 1 1 1 3 3 2 0 0 0 0 0 0
Cytoplasm 171 357 40 59 56 90 97 111 101 52 51 71 266 258 291 318 131 208 165 341 58 155 186 173
Endoplasmic Reticulum 0 4 0 0 0 1 4 11 7 7 7 11 5 7 3 12 5 4 0 2 2 5 3 17
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 1 0 1 0 1 0 0 1 6 3 2 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 3 4 5 6 2 3 7 0 0 0 2 1 0 0 0 0 0 0 1
Nucleus 0 1 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 1 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 4 3 1 0 3 0 0 1 4
Unique Cell Count 172 361 40 60 56 94 111 130 119 63 64 98 272 265 294 329 137 212 169 359 66 163 196 210
Labelled Cell Count 175 365 40 61 56 96 122 138 134 69 73 119 274 266 296 349 148 218 169 359 66 163 196 210


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.9 59.7 34.7 35.5 36.9 31.7 26.4 26.2 26.2 23.8 24.4 24.1 104.8 107.7 103.0 59.1 68.9 69.6 64.7 64.3 60.0
Std Deviation (1e-4) 12.5 15.4 8.6 10.5 14.8 9.5 8.3 8.0 7.5 5.8 8.3 8.1 30.8 35.3 30.8 18.0 22.8 19.5 19.2 17.9 23.7
Intensity Change (Log2) – – – 0.04 0.09 -0.13 -0.39 -0.4 -0.4 -0.54 -0.51 -0.52 1.6 1.64 1.57 0.77 0.99 1.01 0.9 0.89 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Cytoplasm -0.8 0 -1.3 -2.4 -2.6 -2.6 -2.8 -3.0 -3.7 -0.9 -1.0 -0.6 -1.2 -1.3 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.9452 54.2367 54.6729 49.176 47.4498 48.3256 39.1363 52.737 53.997 42.8257 43.172 49.3185 32.2231 51.3594 46.0623 59.2026 42.2677 47.199
Actin 0.0299 0.0008 0.0005 0.0038 0.049 0.0005 0.0243 0.0036 0.0127 0.0021 0.0017 0.0011 0.0653 0.0009 0.0049 0.0002 0.0012 0.0026
Bud 0.0019 0.0015 0.0016 0.0012 0.0005 0.0008 0.0014 0.0021 0.0007 0.0152 0.0019 0.0012 0.0023 0.0006 0.0009 0.0008 0.0004 0.0015
Bud Neck 0.0011 0.0005 0.0007 0.0008 0.002 0.0018 0.0006 0.0002 0.0002 0.0005 0.0003 0.0012 0.0013 0.0004 0.0004 0.0006 0.0005 0.0024
Bud Periphery 0.0029 0.0011 0.0019 0.0011 0.0005 0.0006 0.0016 0.0053 0.0007 0.0359 0.0003 0.0006 0.0016 0.0002 0.0004 0.0003 0.0001 0.0011
Bud Site 0.0016 0.0031 0.0012 0.0008 0.0004 0.0001 0.0034 0.0007 0.0003 0.0019 0.0006 0.0001 0.0014 0.0015 0.0007 0.0001 0.0003 0.0002
Cell Periphery 0.0067 0.0103 0.0068 0.0027 0.0031 0.0005 0.0026 0.0006 0.0005 0.0006 0.0001 0.0003 0.0027 0.0029 0.0015 0.0007 0.0001 0.0016
Cytoplasm 0.6204 0.919 0.8483 0.7517 0.7696 0.883 0.7664 0.9679 0.9392 0.8037 0.9717 0.9019 0.6442 0.9535 0.888 0.6402 0.7276 0.8786
Cytoplasmic Foci 0.0094 0.0036 0.0027 0.0096 0.011 0.0134 0.0241 0.002 0.0024 0.0083 0.0083 0.0047 0.0128 0.0092 0.0124 0.0038 0.0293 0.0073
Eisosomes 0.0004 0 0 0.0001 0.0004 0 0.0003 0.0001 0.0001 0 0 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0823 0.0132 0.0128 0.0185 0.0082 0.0434 0.0707 0.0033 0.0031 0.0166 0.0004 0.0415 0.0371 0.0052 0.0087 0.0074 0.0121 0.0295
Endosome 0.0299 0.0033 0.005 0.0302 0.0177 0.0108 0.0183 0.0016 0.0169 0.0414 0.0053 0.014 0.0163 0.0016 0.0074 0.0382 0.0638 0.0257
Golgi 0.0089 0.0004 0.0004 0.006 0.0017 0.001 0.0056 0.0006 0.0016 0.0019 0.0002 0.0024 0.0107 0.0004 0.0041 0.0005 0.0058 0.0031
Lipid Particles 0.006 0.0013 0.0007 0.0464 0.0032 0.0005 0.0093 0.0001 0.0007 0.0016 0.0001 0.0001 0.0325 0.0022 0.0088 0.0002 0.0053 0.0006
Mitochondria 0.0189 0.0005 0.0022 0.0058 0.0451 0.0056 0.0054 0.0005 0.0009 0.0019 0.0001 0.0069 0.0341 0.0004 0.0244 0.0004 0.0023 0.0033
None 0.0055 0.0006 0.0015 0.001 0.0047 0.0028 0.0105 0.0004 0.0009 0.0039 0.0008 0.0006 0.003 0.0019 0.0036 0.0002 0.0143 0.0007
Nuclear Periphery 0.0293 0.0022 0.0082 0.0052 0.011 0.0044 0.0152 0.0034 0.0025 0.0183 0.0006 0.0023 0.016 0.002 0.0039 0.0062 0.0668 0.01
Nucleolus 0.006 0.0004 0.0019 0.0005 0.0138 0.0002 0.0008 0.0001 0.0001 0.0007 0 0 0.0251 0.0006 0.0006 0.0001 0.0011 0.0001
Nucleus 0.0234 0.0025 0.0055 0.004 0.0075 0.0025 0.0064 0.0015 0.0011 0.0068 0.0009 0.0017 0.022 0.0017 0.0019 0.0046 0.0274 0.0038
Peroxisomes 0.0031 0.0001 0.0001 0.0021 0.001 0.0002 0.0018 0.0001 0.0005 0.0002 0.0001 0 0.008 0.0002 0.0005 0 0.0005 0.0001
Punctate Nuclear 0.002 0.0002 0.0005 0.0005 0.0004 0.0003 0.0074 0.0001 0.0002 0.0019 0.0002 0.0001 0.0047 0.0031 0.0009 0.0001 0.0243 0.0002
Vacuole 0.0907 0.0333 0.0847 0.0914 0.0332 0.0224 0.0204 0.0049 0.0118 0.0288 0.0047 0.0157 0.0505 0.0107 0.0224 0.2515 0.0119 0.0208
Vacuole Periphery 0.0195 0.0021 0.0126 0.0168 0.0157 0.0053 0.0036 0.001 0.0031 0.0077 0.0015 0.0034 0.0082 0.001 0.0038 0.044 0.0049 0.0067

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.1272 46.1708 36.3371 44.7145 36.019 40.6704 26.7014 26.4954 25.0442 29.6225
Translational Efficiency 1.272 1.3451 1.5961 1.1412 1.3206 0.8278 0.8198 0.6362 0.6924 0.6305

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ser3

Ser3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ser3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available