Standard name
Human Ortholog
Description Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0.09 0.08 0.05 0 0 0 0.05 0.05 0 0 0 0 0.11 0.07 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.14 0.12 0 0.08 0.06 0 0.08 0.07 0.09 0.09 0.17 0.22 0.21 0.19 0.23 0.13 0 0 0 0 0 0
Cytoplasm 0.12 0.2 0.12 0.19 0.29 0.29 0.3 0.31 0.31 0.26 0.28 0.34 0.13 0.19 0.22 0.42 0.26 0.35 0 0.05 0 0.05 0 0
Endoplasmic Reticulum 0.87 0.75 0.8 0.73 0.46 0.51 0.3 0.35 0.24 0.3 0.24 0.27 0.79 0.74 0.69 0 0 0 0.91 0.84 0.9 0.77 0.83 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.25 0.1 0.15 0.08 0.38 0.26 0.4 0.39 0 0 0 0.1 0 0 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.05 0.07 0 0.17 0.31 0.28 0.17 0.22 0.16 0.09 0 0 0.09 0.25 0.39 0.36 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 21 10 13 7 12 10 11 8 14 12 11 11 14 5 24 15 23 4 8 4 9 7 4
Bud 1 1 0 2 9 0 4 3 6 10 9 4 0 0 1 3 0 2 1 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 2 1 1
Bud Site 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 21 32 16 1 20 17 12 22 19 23 25 56 98 110 42 50 28 1 4 8 10 7 15
Cytoplasm 41 62 28 27 27 76 82 91 90 69 69 97 43 82 116 93 55 73 6 17 3 22 17 15
Endoplasmic Reticulum 291 230 181 102 43 134 81 104 69 80 60 76 255 322 364 4 8 4 316 274 215 335 315 334
Endosome 1 0 0 0 0 2 1 0 0 0 2 1 0 0 2 3 1 7 1 2 1 4 1 3
Golgi 2 2 0 2 0 1 0 0 0 0 0 0 2 0 1 15 26 8 2 2 1 4 3 3
Mitochondria 4 7 2 8 23 25 40 23 110 69 99 112 0 1 6 23 9 8 3 6 1 25 9 11
Nucleus 0 2 0 0 3 3 4 3 1 5 1 3 1 1 0 5 0 4 0 0 0 0 1 1
Nuclear Periphery 1 0 0 0 0 2 5 0 6 4 1 3 1 1 2 1 0 0 2 0 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 4 0 0 0 0 0 0
Vac/Vac Membrane 18 13 11 10 4 46 85 83 50 58 39 26 9 6 45 55 84 76 4 6 0 14 4 11
Unique Cell Count 334 306 225 139 93 263 273 295 286 263 249 285 322 437 528 224 214 209 350 327 240 437 379 410
Labelled Cell Count 381 359 264 180 117 322 329 331 363 329 315 359 378 525 652 271 250 238 350 327 240 437 379 410


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 9.3 9.9 9.9 6.6 8.4 7.5 7.3 6.3 6.7 6.3 5.8 10.6 10.1 9.4 8.7 11.5 10.4 10.2 10.9 10.4
Std Deviation (1e-4) 1.5 1.5 1.3 1.7 1.3 1.4 1.3 1.1 1.1 1.3 1.0 1.2 1.5 1.3 1.3 1.9 2.6 2.2 1.7 1.7 1.7
Intensity Change (Log2) – – – 0.01 -0.58 -0.24 -0.39 -0.43 -0.64 -0.55 -0.64 -0.75 0.1 0.04 -0.07 -0.18 0.23 0.07 0.05 0.14 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.9 0 0.1 -0.4 -0.4 -1.0 0.4 0.2 -0.3 -0.6 -0.8 0 2.5 1.2 2.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -3.5 -2.4 -3.0 -4.1 -2.4 -2.5 -1.7 -1.9 1.0 2.5 2.1 1.3 2.5 -0.2
Cytoplasm 1.8 3.6 4.4 4.7 4.9 5.1 3.8 4.1 5.6 0.3 2.1 3.0 6.9 3.5 5.5
Endoplasmic Reticulum -1.6 -6.1 -6.8 -11.3 -10.3 -12.6 -11.0 -12.3 -12.1 -0.4 -1.9 -3.2 -16.9 -16.2 -16.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.9 5.4 0
Mitochondria 0 7.2 4.2 5.5 3.6 10.2 7.9 10.3 10.3 0 0 0 4.3 2.2 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.9 0 4.3 7.4 6.8 4.4 5.4 3.8 1.8 -1.3 -2.7 1.7 5.9 8.7 8.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.7 11.2311 10.9952 10.4036 9.8934 11.2026 12.5394 12.8722 12.6039 11.6301 11.3983 12.6698 9.7946 9.423 9.3851 9.8857 9.4595 9.2115
Actin 0.0223 0.0015 0.0118 0.0132 0.0596 0.0013 0.0004 0.0002 0.0006 0.0006 0.0003 0.0003 0.0001 0.0015 0.0014 0.0013 0 0.0002
Bud 0.0001 0 0.0005 0.0001 0.0003 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0
Bud Neck 0.0004 0.0003 0.0044 0.0005 0.0129 0.0014 0.0001 0.0002 0.0002 0.0002 0.0003 0.0003 0.0001 0.0002 0.0004 0.0006 0.0001 0.0003
Bud Periphery 0.0004 0.0001 0.0008 0.0003 0.0007 0.0003 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0001 0 0
Bud Site 0.0006 0.0006 0.0104 0.0001 0.0031 0.0004 0.0003 0.0003 0.0001 0 0 0 0 0.0004 0.0006 0.0001 0 0.0001
Cell Periphery 0.0058 0.0115 0.0101 0.0045 0.0031 0.0033 0.0048 0.0109 0.0031 0.0034 0.0073 0.0017 0.0038 0.0066 0.0038 0.0033 0.0007 0.0018
Cytoplasm 0.0284 0.0565 0.0522 0.0084 0.0312 0.0428 0.0198 0.0267 0.0208 0.0171 0.0203 0.0231 0.0273 0.0716 0.0457 0.0635 0.0046 0.026
Cytoplasmic Foci 0.0097 0.0031 0.0046 0.0014 0.0057 0.0018 0.0008 0.0026 0.0009 0.001 0.0003 0.001 0.0005 0.0019 0.0018 0.0019 0.0001 0.0008
Eisosomes 0.0003 0.0001 0.0005 0.008 0.0008 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.8426 0.8794 0.8613 0.9152 0.7923 0.9269 0.9525 0.9323 0.9616 0.9588 0.9631 0.9533 0.9549 0.888 0.9211 0.9116 0.9899 0.9581
Endosome 0.0189 0.0056 0.0057 0.0068 0.0224 0.0029 0.0039 0.0072 0.0025 0.0046 0.0013 0.0046 0.0017 0.0073 0.0045 0.0028 0.0009 0.0029
Golgi 0.0048 0.0068 0.0095 0.0108 0.0271 0.001 0.0015 0.0008 0.0012 0.001 0.0005 0.0013 0.0005 0.0025 0.003 0.0031 0.0002 0.0006
Lipid Particles 0.0181 0.0238 0.0122 0.0089 0.0098 0.0053 0.0057 0.0074 0.0042 0.0061 0.0028 0.0058 0.0028 0.008 0.0067 0.0052 0.0008 0.0027
Mitochondria 0.0018 0.0002 0.0007 0.0074 0.0065 0.0003 0.0002 0.0002 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0007 0.0002 0 0.0001
None 0.0069 0.0002 0.0014 0.0019 0.004 0.0006 0.0026 0.0009 0.0006 0.0006 0.0002 0.0032 0.0006 0.0008 0.0015 0.0011 0.0001 0.0009
Nuclear Periphery 0.0237 0.0057 0.0066 0.0068 0.0095 0.0035 0.0044 0.0039 0.0023 0.0028 0.0019 0.0027 0.0049 0.0063 0.0037 0.0029 0.0016 0.003
Nucleolus 0.0001 0 0.0002 0.0001 0.0004 0 0 0.0001 0 0 0 0 0 0 0.0001 0 0 0
Nucleus 0.002 0.0005 0.0012 0.0004 0.0012 0.0005 0.0004 0.0003 0.0001 0.0003 0.0001 0.0004 0.0004 0.0006 0.0006 0.0003 0.0001 0.0005
Peroxisomes 0.0066 0.0001 0.0003 0.0007 0.0019 0.0046 0 0 0 0 0 0 0 0.0001 0 0 0 0
Punctate Nuclear 0.0013 0.0002 0.0022 0.0002 0.0032 0.0002 0.0004 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0013 0.0001 0 0.0002
Vacuole 0.0038 0.0035 0.0033 0.0034 0.0033 0.0027 0.0017 0.0047 0.0012 0.0028 0.0009 0.0016 0.0019 0.0033 0.0024 0.0013 0.0008 0.0017
Vacuole Periphery 0.0015 0.0002 0.0003 0.0009 0.0009 0.0002 0.0002 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.7999 48.2841 43.5065 59.1552 70.03 51.4123 57.8285 67.0773 57.5373 60.5769
Translational Efficiency 1.7266 1.9111 1.9811 1.2659 1.4235 2.3995 1.4249 1.5436 1.7497 1.491

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Get2

Get2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Get2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available