Standard name | |
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Human Ortholog | |
Description | Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 5 | 2 | 3 | 1 | 2 | 21 | 15 | 11 | 4 | 11 | 10 |
Bud | 2 | 0 | 0 | 2 | 2 | 3 | 1 | 1 | 3 | 1 | 5 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 4 | 4 | 0 | 2 | 2 |
Bud Neck | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 5 | 0 | 0 | 0 | 2 | 3 | 0 | 9 | 13 | 9 | 6 | 7 | 12 |
Bud Site | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 8 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 3 | 2 | 8 | 2 | 10 | 8 | 5 | 4 | 5 | 2 | 5 | 4 | 3 | 9 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 3 | 1 | 4 | 1 | 5 | 3 | 5 | 2 | 2 | 1 | 2 | 3 | 1 | 0 | 1 | 6 | 6 | 3 | 14 | 10 | 7 | 2 | 7 | 6 |
Golgi | 0 | 8 | 10 | 1 | 6 | 2 | 2 | 2 | 0 | 1 | 0 | 1 | 4 | 10 | 14 | 35 | 18 | 20 | 13 | 19 | 15 | 6 | 16 | 25 |
Mitochondria | 83 | 72 | 27 | 35 | 50 | 83 | 96 | 93 | 81 | 69 | 101 | 95 | 54 | 41 | 44 | 43 | 39 | 24 | 54 | 74 | 31 | 8 | 19 | 31 |
Nucleus | 1 | 1 | 0 | 1 | 1 | 0 | 4 | 5 | 1 | 3 | 1 | 4 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 7 | 6 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 49 | 40 | 20 | 39 | 78 | 72 | 105 | 111 | 84 | 67 | 83 | 101 | 14 | 23 | 9 | 50 | 46 | 47 | 12 | 14 | 8 | 5 | 23 | 21 |
Peroxisomes | 28 | 19 | 30 | 23 | 25 | 11 | 24 | 18 | 17 | 10 | 5 | 15 | 8 | 4 | 19 | 102 | 75 | 47 | 36 | 22 | 29 | 9 | 20 | 37 |
SpindlePole | 12 | 11 | 22 | 26 | 10 | 8 | 10 | 11 | 9 | 8 | 9 | 16 | 2 | 9 | 3 | 76 | 59 | 61 | 27 | 14 | 18 | 7 | 25 | 23 |
Vac/Vac Membrane | 2 | 10 | 1 | 2 | 14 | 8 | 9 | 8 | 3 | 5 | 3 | 6 | 7 | 5 | 5 | 6 | 10 | 14 | 2 | 5 | 3 | 0 | 3 | 4 |
Unique Cell Count | 148 | 132 | 91 | 103 | 160 | 159 | 224 | 212 | 165 | 141 | 171 | 207 | 76 | 80 | 87 | 263 | 201 | 190 | 203 | 201 | 142 | 54 | 147 | 184 |
Labelled Cell Count | 188 | 168 | 118 | 134 | 200 | 197 | 274 | 262 | 207 | 172 | 223 | 260 | 101 | 101 | 105 | 349 | 272 | 233 | 203 | 201 | 142 | 54 | 147 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.5 | 26.2 | 21.9 | 27.0 | 30.2 | 25.9 | 26.5 | 28.4 | 22.8 | 23.7 | 22.2 | 23.6 | 29.6 | 30.0 | 26.5 | 23.0 | 25.5 | 24.1 | – | 28.3 | 31.5 |
Std Deviation (1e-4) | 9.8 | 11.9 | 13.6 | 16.9 | 19.8 | 15.6 | 20.9 | 21.9 | 15.3 | 18.7 | 18.7 | 18.0 | 12.9 | 13.0 | 9.0 | 12.6 | 13.5 | 12.0 | – | 15.3 | 19.0 |
Intensity Change (Log2) | – | – | – | 0.3 | 0.47 | 0.25 | 0.28 | 0.38 | 0.06 | 0.12 | 0.02 | 0.11 | 0.44 | 0.46 | 0.28 | 0.07 | 0.22 | 0.14 | – | 0.37 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.0 | -2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.3 | 0.3 | 1.0 | 0.6 | -0.5 | -0.1 |
Mitochondria | 0.6 | 0.3 | 3.5 | 2.2 | 2.3 | 3.0 | 2.9 | 4.5 | 2.6 | 5.3 | 2.9 | 2.8 | -2.7 | -1.9 | -3.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.4 | 4.2 | 3.7 | 4.1 | 4.9 | 4.5 | 3.9 | 4.2 | 4.3 | -0.6 | 1.0 | -2.1 | -0.6 | 0.2 | 0.5 |
Peroxisomes | -1.7 | -3.2 | -5.4 | -4.7 | -5.3 | -4.5 | -5.1 | -6.8 | -5.7 | -3.4 | -4.6 | -1.7 | 1.0 | 0.7 | -1.4 |
SpindlePole | 0.2 | -4.1 | -4.5 | -5.2 | -4.9 | -4.4 | -4.1 | -4.5 | -3.9 | -4.0 | -2.2 | -4.0 | 0.9 | 0.9 | 1.4 |
Vacuole | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.4339 | 44.629 | 32.7955 | 27.7047 | 31.8545 | 32.4343 | 21.0652 | 44.5181 | 30.2105 | 24.4996 | 29.4591 | 33.5947 | 30.3029 | 55.1425 | 40.9336 | 31.2894 | 42.6744 | 43.5286 |
Actin | 0.0058 | 0.0111 | 0.0071 | 0.0017 | 0.0001 | 0.0008 | 0.0189 | 0.0029 | 0.0008 | 0.0307 | 0.0005 | 0.0003 | 0.0116 | 0.0023 | 0.0087 | 0.0018 | 0.001 | 0.0018 |
Bud | 0.0014 | 0.0084 | 0.002 | 0.0002 | 0.0001 | 0.0029 | 0.0005 | 0.0017 | 0.0073 | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0017 | 0.0019 | 0.0004 | 0.0076 | 0.0002 |
Bud Neck | 0.0007 | 0.0082 | 0.0096 | 0.001 | 0.0264 | 0.0026 | 0.0007 | 0.0026 | 0.003 | 0.0011 | 0.002 | 0.0101 | 0.0017 | 0.0043 | 0.018 | 0.0015 | 0.0116 | 0.0016 |
Bud Periphery | 0.0007 | 0.0033 | 0.0012 | 0.0003 | 0.0001 | 0.0046 | 0.0003 | 0.0011 | 0.0013 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.001 | 0.0008 | 0.0006 | 0.004 | 0.0001 |
Bud Site | 0.0075 | 0.0686 | 0.0307 | 0.0015 | 0.0008 | 0.0027 | 0.0039 | 0.0132 | 0.0144 | 0.0015 | 0.001 | 0.0016 | 0.0124 | 0.0297 | 0.0254 | 0.0016 | 0.0266 | 0.001 |
Cell Periphery | 0.0009 | 0.0093 | 0.004 | 0.0019 | 0.0006 | 0.0042 | 0.0005 | 0.0027 | 0.0016 | 0.0006 | 0.001 | 0.0012 | 0.0009 | 0.0029 | 0.0021 | 0.0071 | 0.0038 | 0.0014 |
Cytoplasm | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasmic Foci | 0.5271 | 0.4243 | 0.5289 | 0.5173 | 0.6217 | 0.6408 | 0.2953 | 0.3874 | 0.4083 | 0.2703 | 0.4337 | 0.46 | 0.4557 | 0.4754 | 0.4578 | 0.1386 | 0.5151 | 0.5526 |
Eisosomes | 0.0005 | 0.0008 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0009 | 0.0004 | 0.0005 | 0.001 | 0.0005 | 0.001 | 0.0007 | 0.0008 | 0.0056 | 0.0012 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0011 | 0.0018 | 0.001 | 0.0012 | 0.0239 | 0.0004 | 0.0121 | 0.0002 | 0.0004 | 0.0018 | 0.0036 | 0.0002 | 0.0017 | 0.0006 | 0.0004 | 0.0034 | 0.0002 | 0.0008 |
Golgi | 0.0021 | 0.0069 | 0.0063 | 0.0038 | 0.0024 | 0.0006 | 0.0088 | 0.0007 | 0.0012 | 0.0078 | 0.0046 | 0.0009 | 0.0036 | 0.0011 | 0.0012 | 0.0017 | 0.0008 | 0.0017 |
Lipid Particles | 0.0876 | 0.0604 | 0.057 | 0.1338 | 0.0681 | 0.0526 | 0.1152 | 0.0539 | 0.0868 | 0.1512 | 0.1456 | 0.0996 | 0.0994 | 0.0616 | 0.0577 | 0.0794 | 0.0769 | 0.0853 |
Mitochondria | 0.1928 | 0.1605 | 0.1548 | 0.1684 | 0.1548 | 0.1275 | 0.3283 | 0.3085 | 0.22 | 0.3318 | 0.2608 | 0.2098 | 0.2359 | 0.139 | 0.2054 | 0.4448 | 0.1274 | 0.1293 |
None | 0.0008 | 0.001 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0037 | 0.0002 | 0.0017 | 0.0001 | 0.0016 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0039 | 0.0003 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0009 | 0.0003 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0682 | 0.1119 | 0.1069 | 0.0706 | 0.0532 | 0.0755 | 0.0539 | 0.1163 | 0.1063 | 0.0477 | 0.0786 | 0.11 | 0.0527 | 0.19 | 0.1205 | 0.1849 | 0.1005 | 0.0996 |
Nucleus | 0.0017 | 0.006 | 0.0042 | 0.0014 | 0.0012 | 0.0023 | 0.0101 | 0.0043 | 0.0036 | 0.0011 | 0.0021 | 0.0021 | 0.0018 | 0.0099 | 0.0033 | 0.004 | 0.005 | 0.0023 |
Peroxisomes | 0.0909 | 0.0585 | 0.0699 | 0.077 | 0.0347 | 0.0633 | 0.1271 | 0.0918 | 0.1244 | 0.1488 | 0.0568 | 0.0984 | 0.1059 | 0.0557 | 0.0835 | 0.0852 | 0.1126 | 0.1106 |
Punctate Nuclear | 0.0011 | 0.0155 | 0.0025 | 0.0021 | 0.0016 | 0.004 | 0.0127 | 0.004 | 0.0095 | 0.0008 | 0.0024 | 0.0006 | 0.009 | 0.0059 | 0.0032 | 0.0002 | 0.0031 | 0.0083 |
Vacuole | 0.0019 | 0.0178 | 0.0052 | 0.005 | 0.0047 | 0.0064 | 0.0012 | 0.0051 | 0.0026 | 0.0012 | 0.0024 | 0.0015 | 0.0012 | 0.006 | 0.0022 | 0.0258 | 0.002 | 0.0015 |
Vacuole Periphery | 0.0031 | 0.0248 | 0.0069 | 0.0122 | 0.0039 | 0.0078 | 0.0024 | 0.0026 | 0.0057 | 0.0019 | 0.0023 | 0.0016 | 0.0024 | 0.0115 | 0.0018 | 0.0169 | 0.0004 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 138.9614 | 154.9938 | 145.8793 | 107.8645 | 116.9449 | 157.997 | 145.7672 | 129.4923 | 123.8207 | 127.8307 |
Translational Efficiency | 0.9772 | 0.9334 | 0.8218 | 1.0664 | 0.9376 | 0.8116 | 0.9594 | 0.8176 | 0.843 | 0.8175 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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