Standard name | |
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Human Ortholog | |
Description | Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 3 | 0 | 3 | 0 | 2 | 12 | 16 | 16 | 14 | 24 | 1 | 4 | 5 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 398 | 266 | 184 | 92 | 130 | 135 | 186 | 169 | 178 | 143 | 164 | 173 | 210 | 294 | 281 | 392 | 304 | 188 | 379 | 244 | 169 | 182 | 171 | 197 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 1 | 2 | 10 | 18 | 14 | 17 | 9 | 7 | 2 | 2 | 0 | 15 | 6 | 10 | 3 | 1 | 3 | 1 | 2 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 2 | 2 | 0 | 1 | 2 | 6 | 2 | 14 | 13 | 15 | 16 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 2 | 0 | 0 | 1 | 6 | 13 | 0 | 5 | 6 | 3 | 0 | 0 | 1 | 2 | 5 | 0 | 0 | 2 | 3 | 0 | 0 | 2 |
Unique Cell Count | 401 | 270 | 189 | 93 | 133 | 139 | 205 | 202 | 212 | 185 | 197 | 209 | 212 | 300 | 282 | 395 | 311 | 189 | 388 | 258 | 188 | 188 | 183 | 212 |
Labelled Cell Count | 404 | 278 | 193 | 94 | 135 | 142 | 213 | 216 | 233 | 200 | 211 | 231 | 213 | 303 | 287 | 412 | 319 | 200 | 388 | 258 | 188 | 188 | 183 | 212 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 51.5 | 47.0 | 33.2 | 30.6 | 28.1 | 23.9 | 17.7 | 18.5 | 17.0 | 15.7 | 15.6 | 14.7 | 45.6 | 44.4 | 43.4 | 54.4 | 65.6 | 69.9 | 44.3 | 42.5 | 45.6 |
Std Deviation (1e-4) | 9.8 | 13.3 | 11.1 | 10.0 | 7.8 | 6.4 | 5.1 | 5.4 | 4.5 | 4.3 | 4.4 | 3.6 | 10.1 | 10.3 | 10.5 | 16.7 | 18.9 | 21.3 | 9.5 | 11.8 | 13.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.24 | -0.47 | -0.91 | -0.84 | -0.97 | -1.08 | -1.09 | -1.17 | 0.46 | 0.42 | 0.39 | 0.71 | 0.98 | 1.07 | 0.41 | 0.36 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 2.2 | 2.8 | 3.1 | 2.6 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 0.2 | -0.1 | -2.7 | -4.6 | -4.5 | -5.9 | -4.7 | -4.8 | 1.3 | 0.5 | 2.2 | 1.8 | 0.3 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.6 | 3.9 | 3.2 | 3.9 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.9 | 3.1 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0074 | 0.0003 | 0.0014 | 0.0024 | 0.0022 | 0.0005 | 0.0107 | 0.0001 | 0.0277 | 0 | 0.0459 | 0.0021 | – | – | – | – | – | – |
Bud | 0.0015 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0006 | 0 | 0.0006 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0006 | 0.0003 | 0.0003 | 0.0005 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | – | – | – | – | – | – |
Bud Periphery | 0.0011 | 0 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0006 | 0 | – | – | – | – | – | – |
Bud Site | 0.0028 | 0.0002 | 0.0016 | 0.0006 | 0.0001 | 0 | 0.0018 | 0 | 0.0017 | 0 | 0.0011 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.8998 | 0.9864 | 0.9915 | 0.9582 | 0.9269 | 0.9935 | 0.9286 | 0.9987 | 0.9177 | 0.9976 | 0.8542 | 0.9774 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0024 | 0.0004 | 0.0005 | 0.0012 | 0.0007 | 0.0004 | 0.0117 | 0.0001 | 0.0093 | 0.0003 | 0.0155 | 0.0011 | – | – | – | – | – | – |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.002 | 0.0007 | 0.0003 | 0.0031 | 0.0057 | 0.0002 | 0.0021 | 0.0001 | 0.0025 | 0.0001 | 0.0032 | 0.0014 | – | – | – | – | – | – |
Endosome | 0.002 | 0.0001 | 0.0001 | 0.0044 | 0.0238 | 0.0002 | 0.0057 | 0 | 0.0118 | 0.0001 | 0.0119 | 0.0046 | – | – | – | – | – | – |
Golgi | 0.0008 | 0 | 0.0001 | 0.0005 | 0.0232 | 0 | 0.0017 | 0 | 0.0077 | 0 | 0.0071 | 0.007 | – | – | – | – | – | – |
Lipid Particles | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.003 | 0 | 0.0015 | 0 | 0.0057 | 0.0019 | – | – | – | – | – | – |
Mitochondria | 0.0138 | 0.0002 | 0.0001 | 0.0048 | 0.0082 | 0.0003 | 0.0014 | 0 | 0.0056 | 0 | 0.0048 | 0.0009 | – | – | – | – | – | – |
None | 0.0029 | 0.0042 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0091 | 0.0001 | 0.0045 | 0.0001 | 0.0098 | 0.0001 | – | – | – | – | – | – |
Nuclear Periphery | 0.012 | 0.0024 | 0.0006 | 0.0083 | 0.0022 | 0.0005 | 0.0037 | 0.0001 | 0.0012 | 0.0003 | 0.012 | 0.0006 | – | – | – | – | – | – |
Nucleolus | 0.0032 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0007 | 0 | 0.0033 | 0 | – | – | – | – | – | – |
Nucleus | 0.0227 | 0.0028 | 0.0022 | 0.0112 | 0.0028 | 0.0027 | 0.0042 | 0.0006 | 0.002 | 0.0013 | 0.0088 | 0.002 | – | – | – | – | – | – |
Peroxisomes | 0.0005 | 0 | 0 | 0.0002 | 0 | 0 | 0.0041 | 0 | 0.001 | 0 | 0.0017 | 0.0001 | – | – | – | – | – | – |
Punctate Nuclear | 0.0065 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0.0095 | 0 | 0.0007 | 0 | 0.0069 | 0.0001 | – | – | – | – | – | – |
Vacuole | 0.0087 | 0.0005 | 0.0004 | 0.0017 | 0.0016 | 0.0003 | 0.0007 | 0 | 0.0018 | 0.0001 | 0.0032 | 0.0002 | – | – | – | – | – | – |
Vacuole Periphery | 0.0073 | 0.0003 | 0.0001 | 0.0011 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.001 | 0 | 0.0031 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 82.1761 | 79.6091 | 79.2448 | 108.3353 | 98.5658 | 74.5326 | 107.8144 | 101.5873 | 97.9861 | 107.1245 |
Translational Efficiency | 2.7095 | 2.8933 | 2.6101 | 1.8379 | 2.0687 | 3.0222 | 2.0824 | 1.8978 | 1.8458 | 1.7498 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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