Standard name
Human Ortholog
Description Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.87 0.87 0.96 0.91 0.94 0.88 0.8 0.89 – 0.81 0.81 0.97 0.99 0.97 0.97 0.95 0.96 0.61 0.64 0.74 0.82 0.82 0.84
Endoplasmic Reticulum 0 0 0 0 0.07 0.05 0.09 0.15 0.08 – 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.2 0.17 0.06 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.1 0.1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.12 0.1 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 3 5 2 – 2 11 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 1 1 0 2 1 0 – 0 0 0 0 1 1 1 0 3 5 7 2 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 4 5 12 10 2 – 9 26 0 0 0 0 2 2 0 0 0 0 0 0
Cytoplasm 140 235 315 218 254 328 347 282 171 – 191 240 190 157 170 136 121 81 112 219 302 92 134 188
Endoplasmic Reticulum 1 11 13 8 20 16 37 52 16 – 26 28 4 2 3 4 3 3 6 4 13 0 7 9
Endosome 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 2 0 0 1 1 0 0 2 0 – 0 2 1 0 1 0 1 0 0 0 1 0 0 2
Mitochondria 0 0 2 0 0 0 0 1 5 – 7 6 0 0 0 1 0 1 0 0 0 0 0 0
Nucleus 1 3 0 0 0 1 2 1 0 – 3 1 1 0 1 0 1 0 36 58 24 6 3 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 6 4 6 0 1 1
Nucleolus 0 0 0 0 0 0 0 1 0 – 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 2 0 0 0
Vac/Vac Membrane 26 26 37 4 9 1 3 9 2 – 7 4 1 1 1 2 5 3 13 40 40 5 6 7
Unique Cell Count 158 269 362 228 280 348 395 351 193 237 298 196 159 175 140 128 84 186 344 407 113 163 226
Labelled Cell Count 171 277 368 233 290 351 406 364 198 247 318 198 160 177 145 134 90 186 344 407 113 163 226


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 77.4 63.4 42.7 42.6 38.5 33.1 33.4 30.7 27.7 – 27.1 30.5 60.1 51.4 43.6 102.8 107.5 111.1 65.6 68.1 67.1
Std Deviation (1e-4) 17.6 15.6 10.8 9.0 9.5 8.2 8.6 7.2 8.6 – 7.6 7.4 13.3 12.2 11.7 37.4 34.3 28.5 18.5 21.4 20.7
Intensity Change (Log2) – – – -0.0 -0.15 -0.37 -0.35 -0.48 -0.62 – -0.65 -0.48 0.49 0.27 0.03 1.27 1.33 1.38 0.62 0.67 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.3 0 0 – 0 5.7 0 0 0 0 0 0
Cytoplasm 3.4 1.5 3.3 0.3 -2.4 0.5 – -2.1 -2.3 3.8 4.2 3.7 3.4 2.3 2.5
Endoplasmic Reticulum -0.1 2.0 0.7 3.2 5.2 2.4 – 3.6 3.1 -1.0 0 -1.2 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -3.9 -3.4 -5.9 -5.8 -4.2 -4.0 – -3.3 -4.7 -4.3 -3.9 -4.1 -3.3 -2.2 -1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0587 0.0001 0.0085 0.0003 0.0782 0.0001 0.0007 0.0002 0.0005 0.0001 0.0098 0.0016 0.0034 0.0275 0 0.0002 0.0003 0.0002
Bud 0.0013 0 0.0004 0.0001 0.0005 0 0.0006 0 0.0002 0.0002 0.0008 0 0.0012 0.0034 0 0.0001 0 0
Bud Neck 0.0045 0.0002 0.0006 0.0003 0.001 0.0005 0.0004 0.0002 0.0004 0.0002 0.0008 0.0005 0.0008 0.0005 0.0001 0.0002 0.0009 0.0002
Bud Periphery 0.0013 0 0.0005 0.0001 0.0004 0 0.0006 0 0.0002 0.0002 0.0006 0 0.0006 0.0077 0 0 0 0
Bud Site 0.002 0 0.0045 0.0006 0.0008 0 0.0006 0 0.0016 0.0004 0.003 0 0.0016 0.0065 0 0 0 0
Cell Periphery 0.0016 0 0.0005 0 0.0006 0 0.0005 0.001 0.0009 0.0016 0.0012 0 0.0011 0.0007 0 0 0 0
Cytoplasm 0.2429 0.6131 0.5897 0.6722 0.5097 0.4305 0.5605 0.7358 0.7205 0.736 0.7321 0.6474 0.5399 0.6198 0.7973 0.8471 0.6917 0.4051
Cytoplasmic Foci 0.0293 0 0.0055 0.0001 0.0065 0.0001 0.0021 0.0001 0.0017 0.0012 0.0178 0.0002 0.0864 0.0015 0.0001 0.0004 0 0.0001
Eisosomes 0.0014 0 0.0005 0 0.0003 0 0.0001 0 0 0.0001 0.0003 0 0.0002 0.001 0 0 0 0
Endoplasmic Reticulum 0.0081 0.0006 0.0019 0.0005 0.0023 0.0008 0.0044 0.0009 0.0018 0.0011 0.0046 0.0015 0.0323 0.0044 0.0001 0.0009 0.0004 0.0004
Endosome 0.0136 0.0001 0.0036 0.0002 0.0076 0.0002 0.0016 0.0001 0.0013 0.0015 0.0203 0.0021 0.059 0.0119 0 0.0006 0 0.0001
Golgi 0.0104 0 0.0013 0 0.0082 0 0.0001 0 0.0018 0.0009 0.0044 0.0004 0.0021 0.0027 0 0 0 0
Lipid Particles 0.0123 0 0.0036 0 0.0083 0 0.0007 0.0003 0.0072 0.0218 0.0075 0.0001 0.0081 0.0022 0 0 0 0
Mitochondria 0.0145 0.0002 0.0073 0.0003 0.0053 0.0001 0.0026 0.0001 0.0065 0.0027 0.0054 0.0008 0.0036 0.1789 0 0.0002 0.0001 0.0002
None 0.0294 0.0001 0.0049 0.0003 0.0031 0.0001 0.0024 0.0002 0.0007 0.0015 0.0094 0.0002 0.1561 0.0039 0.0001 0.0002 0.0001 0.0001
Nuclear Periphery 0.139 0.0793 0.0836 0.0634 0.0869 0.1374 0.1251 0.0449 0.0582 0.0497 0.0302 0.0693 0.0423 0.0406 0.0462 0.0567 0.0433 0.143
Nucleolus 0.0291 0.0002 0.0045 0.001 0.0215 0.0002 0.0045 0.0003 0.0028 0.0101 0.0052 0.0001 0.0032 0.006 0 0.0001 0 0.0002
Nucleus 0.3657 0.3029 0.2559 0.2573 0.2227 0.4269 0.2788 0.2139 0.1853 0.1614 0.1068 0.2733 0.0189 0.0624 0.1557 0.0897 0.2622 0.448
Peroxisomes 0.002 0 0.0009 0 0.0056 0 0.0001 0 0.0008 0.0018 0.0014 0 0.0013 0.0016 0 0 0 0
Punctate Nuclear 0.0139 0.0002 0.0015 0.0014 0.0045 0.0003 0.0015 0.0003 0.0008 0.0005 0.0108 0.001 0.0104 0.0025 0.0002 0.0007 0.0006 0.0012
Vacuole 0.0119 0.0021 0.0092 0.0011 0.0132 0.0014 0.0078 0.0016 0.0038 0.005 0.0173 0.0007 0.0228 0.0057 0.0001 0.0012 0.0001 0.0004
Vacuole Periphery 0.0072 0.0009 0.0107 0.0008 0.0128 0.0014 0.0043 0.0002 0.0028 0.002 0.0103 0.0006 0.0047 0.0086 0 0.0016 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 342.7091 352.1085 368.1569 356.9107 285.7372 238.9056 310.7312 264.9605 236.2419 254.8009
Translational Efficiency 1.1558 1.3382 1.2768 1.1469 1.2454 1.3262 1.167 1.083 1.1693 1.0741

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
Localization
Cell Percentages cytoplasm (40%), ER (3%), mixed (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kap123

Kap123


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap123-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available