Standard name
Human Ortholog
Description Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 – 0 0 0 0.19 0.15 0.07 0.07 0.06 0.08 0 0 0 0 0 0.05
Bud 0 0 0 0.05 0.07 0.05 0.09 0.07 – 0.09 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.06 0 0.05 0 – 0 0 0 0.05 0 0.06 0.25 0.28 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 – 0 0 0 0.06 0.09 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.34 0.05 0.26 0.33 0.41 0.22 0.25 0.17 – 0 0 0.06 0.27 0.2 0.31 0.34 0.31 0.46 0.26 0.37 0.25 0.2 0.18 0.15
Golgi 0.23 0.05 0.17 0.08 0.06 0 0 0 – 0 0 0 0.38 0.5 0.38 0 0 0.1 0.54 0.38 0.46 0.52 0.55 0.59
Mitochondria 0.6 0.86 0.61 0.65 0.45 0.69 0.58 0.65 – 0.87 0.9 0.84 0.26 0.19 0.18 0.13 0.15 0.09 0.07 0.13 0 0 0.05 0.08
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.08 0.16 0.2 0.33 0.21 – 0.13 0.05 0.14 0 0 0 0 0.09 0 0.08 0.06 0.2 0.18 0.16 0.09
SpindlePole 0.07 0 0.05 0.07 0.09 0.1 0.18 0.14 – 0.1 0.07 0.12 0.08 0.06 0.07 0.31 0.31 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0.12 0.06 0.08 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 19 5 2 5 1 0 4 – 1 7 2 30 37 16 17 12 11 2 2 16 10 16 28
Bud 4 12 10 11 25 26 75 70 – 62 62 76 4 3 4 3 3 1 0 1 0 2 0 0
Bud Neck 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 10 6 – 7 12 10 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 2 4 2 – 3 3 3 1 2 0 1 0 0 0 0 0 0 0 0
Cytoplasm 4 18 16 10 23 11 40 38 – 19 25 19 8 10 14 59 58 31 0 2 0 0 0 1
Endoplasmic Reticulum 8 19 8 2 5 5 4 4 – 4 10 2 10 22 8 10 11 5 0 1 0 0 2 9
Endosome 71 19 98 80 146 122 200 159 – 22 11 44 43 50 73 80 64 62 61 137 102 77 90 76
Golgi 47 19 64 19 23 4 1 1 – 1 2 5 61 122 87 2 3 13 126 141 190 195 274 304
Mitochondria 125 302 228 158 159 387 463 607 – 634 606 653 42 47 42 30 30 12 16 48 11 16 24 44
Nucleus 0 1 1 0 0 2 2 4 – 2 0 0 0 1 1 15 12 3 0 1 0 0 0 0
Nuclear Periphery 0 3 2 2 0 3 0 4 – 1 2 1 1 0 1 0 1 2 0 0 0 0 0 0
Nucleolus 3 5 5 3 3 6 8 21 – 7 10 5 1 0 1 0 2 0 0 0 0 0 0 0
Peroxisomes 3 2 41 20 58 112 265 200 – 96 37 109 3 3 3 2 19 0 18 23 83 68 77 46
SpindlePole 15 13 20 17 31 56 147 127 – 73 47 95 13 15 17 74 63 15 2 5 2 1 3 1
Vac/Vac Membrane 10 8 10 1 6 9 21 13 – 10 7 12 9 11 27 15 17 20 2 7 0 0 3 1
Unique Cell Count 208 353 371 244 356 564 798 939 728 677 782 162 244 232 236 205 134 235 376 413 376 496 519
Labelled Cell Count 298 441 508 325 484 749 1240 1260 942 841 1036 226 323 294 311 295 175 235 376 413 376 496 519


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.9 6.3 6.0 5.8 4.7 4.7 4.7 – 4.0 4.2 4.2 7.7 7.9 8.0 5.5 6.0 7.4 8.4 8.2 8.9
Std Deviation (1e-4) 0.7 1.1 1.3 1.1 1.4 0.8 2.9 1.2 – 0.9 0.8 0.8 0.9 0.9 0.9 1.4 1.7 1.8 1.4 1.0 1.7
Intensity Change (Log2) – – – -0.08 -0.14 -0.42 -0.43 -0.44 – -0.65 -0.59 -0.59 0.28 0.31 0.33 -0.2 -0.07 0.23 0.41 0.37 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 7.4 6.6 3.6 3.8 3.1 0
Bud 1.2 2.7 0 0 0 – 0 0 0 0 -1.2 -0.8 -1.2 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 1.3 -2.1 0.5 -0.2 – -1.5 -0.5 -1.7 0.3 -0.1 0.9 7.6 8.2 6.4
Endoplasmic Reticulum 0 -0.8 0 0 0 – 0 0 0 2.4 3.9 1.0 1.5 2.1 0
Endosome 1.7 4.2 0 0 0 – 0 0 0 0 -1.7 1.3 2.0 1.2 4.2
Golgi -3.4 -4.5 0 0 0 – 0 0 0 5.1 8.7 5.6 -6.3 -5.7 -2.1
Mitochondria 0.8 -4.5 0 0 0 – 0 0 0 -7.5 -10.3 -10.4 -11.8 -10.8 -10.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 4.6 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -1.2 2.1 0 0 0 – 0 0 0 -3.5 -4.6 -4.5 -4.8 -0.7 -4.0
SpindlePole 0.8 1.8 0 0 0 – 0 0 0 1.2 0.4 1.0 8.6 8.3 2.3
Vacuole 0 -0.9 -1.2 -0.1 -1.6 – -1.6 -2.0 -1.3 1.6 1.2 4.5 2.2 3.0 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5488 3.3919 3.0865 3.531 2.8121 3.2841 3.5537 4.3383 3.8981 3.5569 3.6918 3.8688 2.6452 4.0718 3.5055 3.2896 3.236 3.3414
Actin 0.0418 0.0087 0.0133 0.002 0.0053 0.0279 0.0283 0.0086 0.0352 0.0433 0.0222 0.0335 0.0331 0.0173 0.0184 0.001 0.0007 0.0281
Bud 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0005 0.0003 0.0051 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0.0001
Bud Neck 0.0019 0.0003 0.0004 0.0003 0.0003 0.0009 0.0002 0.0002 0.0004 0.0021 0.0002 0.0004 0.0003 0.0003 0.0003 0.0001 0.0001 0.0005
Bud Periphery 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0006 0.0003 0.0102 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Bud Site 0.0008 0.0004 0.0005 0.0001 0.0002 0.0003 0.0004 0.0037 0.002 0.0037 0.0003 0.0002 0.0006 0.0006 0.0006 0.0001 0 0.0002
Cell Periphery 0.0002 0 0.0001 0 0 0.0001 0.0001 0.0003 0.0001 0.0004 0 0 0 0 0 0 0 0
Cytoplasm 0.005 0.0044 0.0029 0.002 0.0009 0.0036 0.0125 0.0137 0.0038 0.0018 0.0012 0.0016 0.0024 0.0014 0.0014 0.0007 0.0009 0.0027
Cytoplasmic Foci 0.1241 0.202 0.1618 0.114 0.147 0.132 0.1346 0.1898 0.1642 0.146 0.2041 0.1512 0.1762 0.2467 0.1558 0.1838 0.1676 0.1549
Eisosomes 0.0003 0 0 0 0 0.0001 0.0001 0.0001 0.0003 0.0006 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0059 0.0007 0.0008 0.0007 0.0003 0.0007 0.0009 0.0018 0.0006 0.001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002 0.0004 0.0003
Endosome 0.2124 0.2601 0.2276 0.3492 0.2179 0.185 0.1719 0.1528 0.1875 0.2197 0.1813 0.1453 0.1442 0.1731 0.1756 0.1619 0.2512 0.1366
Golgi 0.373 0.4255 0.4785 0.4838 0.5316 0.5103 0.3426 0.5038 0.4379 0.3985 0.3682 0.4577 0.329 0.3654 0.4756 0.4255 0.486 0.453
Lipid Particles 0.0961 0.0297 0.0293 0.0245 0.0249 0.0477 0.0928 0.0376 0.0236 0.0442 0.0608 0.0649 0.0732 0.0266 0.026 0.0862 0.0348 0.0535
Mitochondria 0.0162 0.0066 0.0239 0.0068 0.0095 0.0234 0.0124 0.0126 0.0326 0.0753 0.0035 0.0125 0.0131 0.0051 0.0173 0.0045 0.004 0.0086
None 0.0034 0.0085 0.0001 0.0001 0.0001 0.0002 0.0003 0.0118 0.0008 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001 0 0 0.001
Nuclear Periphery 0.0086 0.0002 0.0003 0.0002 0.0001 0.0002 0.0087 0.0005 0.0002 0.0008 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Nucleolus 0.0004 0.0001 0 0 0 0 0.0001 0.0002 0.0001 0.0002 0 0 0 0 0 0 0 0
Nucleus 0.0046 0.0001 0.0001 0 0 0.0001 0.0147 0.0004 0.0001 0.0005 0 0 0 0 0 0 0 0
Peroxisomes 0.0938 0.0504 0.056 0.0097 0.0598 0.0647 0.1757 0.0561 0.1081 0.0315 0.1553 0.1298 0.2253 0.1617 0.1264 0.1348 0.0519 0.1585
Punctate Nuclear 0.0074 0.0004 0.0003 0.0001 0.0002 0.0004 0.001 0.0004 0.0004 0.0002 0.0011 0.0003 0.0005 0.0003 0.0002 0.0002 0.0002 0.0004
Vacuole 0.002 0.0009 0.002 0.0028 0.0006 0.0011 0.0011 0.0034 0.0008 0.0095 0.0003 0.0006 0.0005 0.0004 0.0008 0.0003 0.0007 0.0005
Vacuole Periphery 0.0017 0.0009 0.0018 0.0034 0.0012 0.0013 0.0014 0.0013 0.001 0.0051 0.0004 0.0012 0.0006 0.0004 0.0008 0.0005 0.0011 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0114 17.2069 15.529 27.0846 29.0035 16.6381 22.5703 22.8501 20.045 33.3831
Translational Efficiency 0.5019 0.4525 0.6229 0.3444 0.3806 0.7597 0.449 0.3338 0.3957 0.3494

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
Localization
Cell Percentages Golgi (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tmn3

Tmn3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tmn3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available