Standard name
Human Ortholog
Description Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.53 0.52 0.43 0.26 0.26 0.21 0.08 0.12 0.13 0.13 0.12 0.09 0.55 0.48 0.41 0.41 0.34 0.35 0.41 0.31 0.3 0.2 0.2 0.29
Bud 0.15 0.19 0.33 0.23 0.22 0.12 0.1 0.13 0.07 0.09 0.17 0.06 0.2 0.21 0.19 0.21 0.2 0.14 0.23 0.24 0.22 0.37 0.31 0.34
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.22 0.17 0.26 0.3 0.24
Bud Site 0.16 0.11 0 0 0.09 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0.11 0.09 0.05 0.06 0.05 0.06 0.05 0.07 0.12 0.11 0.12 0.15 0.17 0.2 0.32 0.16 0.24 0.14 0.06 0 0 0 0 0
Cytoplasm 0.25 0.3 0.28 0.62 0.51 0.53 0.59 0.58 0.4 0.35 0.42 0.39 0.28 0.39 0.41 0.34 0.4 0.39 0.06 0.09 0.17 0.09 0.08 0.05
Endoplasmic Reticulum 0 0.13 0.05 0.09 0.07 0.09 0.07 0.05 0.06 0.07 0 0.08 0.07 0.11 0.07 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.28 0.14 0.11 0.24 0.26 0.31 0.51 0.55 0.46 0.42 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0.1 0 0 0.05 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 133 78 26 18 50 35 25 42 29 42 20 23 146 216 216 118 52 125 129 54 29 44 80 156
Bud 37 28 20 16 42 21 30 48 17 27 27 15 52 95 98 60 30 50 73 42 21 83 123 182
Bud Neck 2 0 0 0 1 1 0 0 0 1 1 0 0 0 0 0 3 0 49 37 16 58 120 131
Bud Site 41 17 1 1 17 8 9 8 10 10 3 12 11 11 6 4 2 5
Cell Periphery 27 14 3 4 9 10 16 25 28 36 19 37 46 90 169 45 36 52 17 6 2 1 2 8
Cytoplasm 62 45 17 43 97 90 178 211 93 111 68 94 74 173 216 100 61 139 18 15 16 20 32 26
Endoplasmic Reticulum 10 19 3 6 14 15 20 19 14 22 7 19 18 48 35 16 11 10 9 2 0 1 12 11
Endosome 4 2 0 0 1 1 8 4 0 0 0 1 2 0 1 7 3 16 6 1 2 3 10 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 3 12 2 0 0 0 3 0
Mitochondria 6 7 17 10 20 41 80 113 118 173 74 101 8 13 28 14 8 13 4 2 3 0 5 5
Nucleus 2 0 0 0 0 2 1 3 3 3 2 0 1 0 1 2 1 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 1 3 0 0 1 1 0 0 0 1 0 0 3 6
Nucleolus 1 0 0 0 0 1 4 2 4 1 1 1 0 1 0 1 1 1 0 0 0 0 0 0
Peroxisomes 24 15 0 0 9 1 7 2 3 4 2 3 22 8 3 11 2 7 0 0 0 0 0 1
SpindlePole 14 1 0 1 3 3 5 4 0 1 0 13 9 7 2 10 8 28 0 0 3 1 2 2
Vac/Vac Membrane 4 0 0 0 3 2 9 10 4 8 2 21 2 1 3 10 5 33 1 3 0 6 2 3
Unique Cell Count 250 151 61 69 189 169 303 365 231 315 161 243 264 448 523 290 152 361 315 173 98 223 404 545
Labelled Cell Count 367 226 87 99 266 231 392 492 323 440 227 343 391 663 779 407 226 492 315 173 98 223 404 545


Bud, Bud Neck, Cell Periphery, Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 7.7 5.5 5.8 5.6 5.1 5.0 4.9 4.6 4.7 5.0 7.6 8.4 7.9 7.0 11.0 11.4 12.7 8.4 8.3 8.5
Std Deviation (1e-4) 1.6 1.5 0.7 0.7 1.1 0.8 1.1 0.9 0.8 0.9 1.3 6.2 1.6 1.6 1.5 2.7 2.8 3.3 1.2 1.5 1.3
Intensity Change (Log2) 0.09 0.02 -0.1 -0.13 -0.16 -0.24 -0.22 -0.15 0.48 0.62 0.52 0.36 1.0 1.05 1.21 0.62 0.59 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -2.4 -3.3 -7.1 -6.1 0 0 -5.0 -6.3 1.8 0.8 -0.2 -0.3 -1.2 -1.2
Bud -1.2 -1.7 -3.6 -4.7 -3.9 0 0 -2.6 -5.8 -2.2 -2.0 -2.6 -2.0 -2.0 -3.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1.5 2.1 2.5 2.9 4.4 2.2 3.2 2.0
Cytoplasm 3.9 3.2 3.4 4.4 4.3 1.8 1.1 2.0 1.6 0 1.6 2.0 1.0 1.7 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1.4 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.9 -3.3 -0.6 -0.2 0.5 0 0 2.4 2.0 0 0 0 -5.8 -4.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5801 4.8652 4.0844 4.4785 3.1284 4.2669 4.5929 5.7732 5.6329 5.3741 5.6624 5.5062 2.4985 3.7343 3.4824 2.8827 2.5785 3.0987
Actin 0.2684 0.1103 0.2355 0.2733 0.3043 0.2195 0.21 0.0838 0.2019 0.2507 0.2979 0.158 0.1749 0.1648 0.1978 0.3976 0.2085 0.1928
Bud 0.0088 0.0378 0.1723 0.088 0.0155 0.009 0.0247 0.0155 0.2275 0.0888 0.0528 0.0268 0.0093 0.0234 0.1777 0.0586 0.0828 0.005
Bud Neck 0.0725 0.0064 0.0159 0.0042 0.0671 0.5149 0.1028 0.0116 0.0137 0.0071 0.0037 0.5784 0.0788 0.0089 0.0105 0.0012 0.1078 0.4478
Bud Periphery 0.0189 0.0527 0.3639 0.4732 0.1102 0.0379 0.0505 0.0156 0.3909 0.3331 0.4001 0.0897 0.0706 0.0365 0.3906 0.4575 0.3696 0.0152
Bud Site 0.1568 0.3234 0.0801 0.0176 0.0286 0.0124 0.1886 0.4556 0.0595 0.0175 0.0115 0.0254 0.1223 0.3263 0.0987 0.054 0.0336 0.0127
Cell Periphery 0.0184 0.025 0.009 0.0139 0.0044 0.0079 0.009 0.0121 0.0038 0.0066 0.0411 0.0095 0.0857 0.0116 0.0067 0.0056 0.0286 0.0048
Cytoplasm 0.2456 0.4172 0.0581 0.0376 0.0074 0.1679 0.2163 0.376 0.0673 0.0219 0.027 0.0603 0.3524 0.3713 0.088 0.0164 0.1315 0.2653
Cytoplasmic Foci 0.0385 0.0086 0.0094 0.0065 0.0162 0.008 0.04 0.0097 0.0057 0.03 0.0564 0.0168 0.0157 0.0038 0.002 0.0036 0.0259 0.0156
Eisosomes 0.002 0.0009 0.0004 0.0009 0.003 0.0006 0.002 0.0003 0.0005 0.0005 0.0004 0.0004 0.0064 0.0007 0.0007 0.0012 0.0007 0.0008
Endoplasmic Reticulum 0.021 0.002 0.0011 0.0012 0.008 0.0009 0.0095 0.0017 0.0004 0.0021 0.0005 0.0017 0.0075 0.0026 0.0017 0.0002 0.0014 0.0018
Endosome 0.0379 0.002 0.0073 0.0143 0.0633 0.002 0.0395 0.0015 0.0023 0.0686 0.0394 0.0059 0.015 0.0017 0.0009 0.0001 0.0016 0.0011
Golgi 0.0221 0.0038 0.0069 0.0214 0.0253 0.0039 0.0194 0.0024 0.003 0.0399 0.0416 0.0143 0.0075 0.0026 0.0007 0.0007 0.0027 0.0035
Lipid Particles 0.0106 0.0006 0.0056 0.0094 0.0316 0.0027 0.0085 0.0006 0.0013 0.0305 0.0042 0.0007 0.0084 0.0005 0.0001 0 0.0002 0.0025
Mitochondria 0.0082 0.0006 0.0174 0.0062 0.0674 0.005 0.0132 0.0004 0.0142 0.024 0.0053 0.0029 0.0058 0.0004 0.0006 0.0005 0.0009 0.0007
None 0.0097 0.0047 0.0016 0.0263 0.0191 0.0016 0.0287 0.0085 0.0009 0.001 0.0004 0.0006 0.0082 0.0418 0.0213 0.0008 0.0019 0.0172
Nuclear Periphery 0.0282 0.0004 0.0006 0.0011 0.0556 0.0007 0.0116 0.0003 0.0005 0.0078 0.0002 0.0002 0.0014 0.0004 0.0002 0.0001 0.0003 0.0001
Nucleolus 0.0008 0.0001 0.0016 0.0001 0.0164 0.0001 0.0008 0.0001 0.0002 0.0041 0.0001 0.0001 0.0004 0.0001 0 0 0.0001 0.0001
Nucleus 0.007 0.0007 0.0009 0.0004 0.0254 0.0004 0.0029 0.0008 0.0025 0.0297 0.0001 0.0006 0.0012 0.0007 0.0003 0.0001 0.0004 0.0003
Peroxisomes 0.0094 0.001 0.0065 0.002 0.074 0.003 0.0062 0.0009 0.0026 0.0199 0.0152 0.0062 0.0152 0.0002 0.0006 0.0007 0.0004 0.0118
Punctate Nuclear 0.0097 0.0003 0.0037 0.0007 0.044 0.0006 0.0117 0.0006 0.0002 0.0023 0.0006 0.0003 0.0043 0.0006 0.0001 0.0001 0.0004 0.0003
Vacuole 0.0031 0.0012 0.0018 0.0011 0.0066 0.0008 0.0027 0.0019 0.001 0.009 0.0011 0.0009 0.0085 0.001 0.0006 0.0007 0.0007 0.0004
Vacuole Periphery 0.0024 0.0001 0.0004 0.0006 0.0066 0.0002 0.0013 0.0001 0.0002 0.0049 0.0004 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.5586 38.2169 27.1868 24.2987 26.1111 25.0715 36.8049 32.6541 25.6521 29.2655
Translational Efficiency 1.2231 1.1725 1.2404 1.0896 1.2927 1.1935 0.9993 0.9607 1.0136 0.9597

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
Localization
Cell Percentages actin (36%), cytoplasm (10%), mixed (23%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00023)
actin - MA (9.2e-05)
Subcompartmental Group N/A

Boi2

Boi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Boi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available