Standard name
Human Ortholog
Description Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.15 0.14 0.16 0.2 0.17 0.25 – 0.23 0.23 0.25 0.18 0.25 0.27 0.35 0.11 0.12 0.14 0.05 0.08 0 0.06 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0.06 0.06 0 0.05 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.31 0 0.09 0.27 0.24 0.3 – 0.72 0.6 0.64 0.62 0 0 0 0.06 0 0 0 0 0 0 0.05 0
Nucleus 0.64 0.57 0.75 0.64 0.52 0.51 0.44 – 0.23 0.23 0.24 0.26 0.7 0.68 0.5 0.82 0.81 0.82 0.48 0.54 0.59 0.51 0.39 0.33
Nuclear Periphery 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.05 0.08 0.07 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.15 0.19
Vac/Vac Membrane 0.07 0.1 0 0.07 0.11 0.1 0.07 – 0.08 0.08 0.07 0.07 0.05 0 0.13 0.05 0.06 0 0.28 0.18 0.17 0.2 0.2 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 7
Bud 0 0 0 1 0 1 3 – 14 3 4 5 0 0 0 0 0 0 5 4 3 13 12 25
Bud Neck 0 0 3 2 3 0 3 – 3 3 0 0 0 1 0 0 0 0 1 1 0 2 4 8
Bud Site 3 0 0 2 1 2 3 – 13 4 9 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 19 46 34 36 35 41 73 – 90 55 63 34 154 206 299 57 54 58 16 30 11 40 37 39
Endoplasmic Reticulum 0 2 0 1 0 0 1 – 0 0 0 6 1 0 1 16 10 9 0 0 2 2 4 3
Endosome 16 2 15 13 1 12 11 – 3 1 2 1 6 10 44 6 8 3 14 10 5 13 32 42
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 1 0 2 1 2 3 1 1 5 2 4
Mitochondria 38 95 2 20 47 59 87 – 279 142 162 119 4 3 4 32 11 6 5 13 12 29 38 29
Nucleus 154 177 186 144 91 123 127 – 88 55 60 50 427 517 434 435 361 346 159 202 200 339 290 261
Nuclear Periphery 13 1 0 0 0 1 0 – 0 1 1 1 0 0 1 2 3 1 1 0 1 2 4 4
Nucleolus 27 0 0 1 0 2 0 – 3 1 1 0 0 1 1 3 1 1 9 11 11 23 27 28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 2
SpindlePole 3 3 17 11 14 17 11 – 10 8 9 5 6 20 9 5 5 6 13 24 19 49 114 146
Vac/Vac Membrane 18 32 11 16 20 24 20 – 30 18 17 13 28 34 111 26 27 19 91 69 58 131 148 155
Unique Cell Count 242 308 249 224 174 242 289 386 238 253 191 609 763 860 532 446 424 332 378 340 672 751 791
Labelled Cell Count 292 358 269 248 212 282 339 533 291 328 236 627 793 907 584 481 451 332 378 340 672 751 791


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 5.6 5.2 4.5 4.6 4.3 – 3.5 3.8 3.7 3.7 5.7 5.7 5.4 5.6 7.2 6.7 6.2 6.3 6.5
Std Deviation (1e-4) 0.8 0.6 1.2 1.4 1.2 1.2 1.2 – 1.4 0.8 1.2 0.7 1.3 1.4 1.4 1.1 1.6 1.3 1.5 1.8 1.7
Intensity Change (Log2) – – – -0.12 -0.31 -0.29 -0.4 – -0.67 -0.55 -0.62 -0.62 0.01 0.03 -0.04 -0.01 0.36 0.25 0.14 0.17 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.3 0.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7344 4.1359 4.0219 3.3829 3.9863 3.7352 3.1707 2.7462 2.7554 2.3951 1.75 2.8099 -0.4389 0.1583 0.0592 0.1054 -0.1915 0.0235
Actin 0.0472 0.0014 0.0169 0.0046 0.0119 0.0069 0.0317 0.0008 0.0013 0.041 0.0007 0.0166 0.045 0.0001 0.0227 0.0046 0.0322 0.0001
Bud 0.0012 0.0019 0.0065 0.0002 0.0004 0.0001 0.001 0.0001 0.0002 0.0003 0.0001 0.0003 0.0007 0.0001 0.0016 0.0012 0.0007 0.0001
Bud Neck 0.007 0.003 0.0025 0.0008 0.0023 0.0038 0.0044 0.0002 0.0008 0.0004 0.001 0.0033 0.0013 0.0006 0.0007 0.0026 0.0019 0.0037
Bud Periphery 0.0021 0.0008 0.0041 0.0002 0.0007 0.0004 0.0017 0.0001 0.0002 0.0008 0.0001 0.0009 0.0012 0 0.0011 0.0012 0.0014 0.0001
Bud Site 0.0151 0.0066 0.0086 0.0008 0.0017 0.0004 0.0071 0.0005 0.0013 0.0006 0.0002 0.0004 0.0031 0.001 0.0099 0.0286 0.006 0.0002
Cell Periphery 0.0006 0.0002 0.0011 0 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0004 0.0005 0
Cytoplasm 0.0406 0.0444 0.05 0.0321 0.0188 0.031 0.0765 0.1222 0.1085 0.1208 0.0492 0.0471 0.1051 0.1121 0.0921 0.1014 0.0591 0.0918
Cytoplasmic Foci 0.0234 0.0058 0.0093 0.0137 0.0223 0.0027 0.0436 0.0088 0.0222 0.0087 0.0021 0.0014 0.03 0.0011 0.0144 0.0269 0.0424 0.0015
Eisosomes 0.0008 0.0003 0.0006 0 0.0003 0.0001 0.0005 0.0001 0.0001 0.0005 0 0.0002 0.0005 0.0001 0.0003 0 0.0012 0
Endoplasmic Reticulum 0.0061 0.0016 0.0041 0.0007 0.0026 0.0009 0.0059 0.0037 0.0032 0.0023 0.0023 0.0008 0.0087 0.0026 0.0018 0.0057 0.0027 0.0013
Endosome 0.0188 0.0035 0.0192 0.0084 0.0254 0.0017 0.0618 0.0113 0.0305 0.0049 0.0183 0.0016 0.022 0.0009 0.0046 0.007 0.0331 0.0013
Golgi 0.0084 0.0012 0.0154 0.0049 0.0142 0.0006 0.0289 0.002 0.0114 0.0078 0.0004 0.0007 0.0081 0 0.0035 0.0015 0.0073 0
Lipid Particles 0.0213 0.0137 0.01 0.0098 0.0446 0.0022 0.029 0.0017 0.0143 0.0086 0.0011 0.0004 0.0162 0.0001 0.0086 0.0025 0.0206 0.0002
Mitochondria 0.021 0.0145 0.0194 0.0014 0.0341 0.008 0.0115 0.0021 0.0029 0.0773 0.0009 0.0084 0.0126 0.0004 0.0025 0.0014 0.021 0.0004
None 0.1166 0.159 0.1704 0.1267 0.0835 0.1211 0.1965 0.4509 0.2624 0.348 0.325 0.2169 0.0934 0.1982 0.2086 0.0584 0.108 0.0592
Nuclear Periphery 0.0264 0.0099 0.012 0.0157 0.0246 0.0057 0.0163 0.006 0.0112 0.0418 0.0327 0.0168 0.0262 0.0104 0.0105 0.0329 0.0166 0.0064
Nucleolus 0.0064 0.0225 0.0067 0.0059 0.0069 0.0195 0.009 0.0017 0.0109 0.0026 0.0058 0.0092 0.0049 0.0067 0.0052 0.0038 0.0048 0.0068
Nucleus 0.555 0.645 0.5743 0.6835 0.6311 0.7343 0.3912 0.3743 0.456 0.2997 0.5319 0.6435 0.5631 0.6385 0.571 0.6765 0.5523 0.7997
Peroxisomes 0.0125 0.0074 0.0057 0.0046 0.0207 0.0017 0.0219 0.0019 0.0025 0.0216 0.0002 0.0008 0.0089 0 0.012 0.0065 0.0548 0
Punctate Nuclear 0.0639 0.0553 0.0603 0.0854 0.0508 0.0574 0.0485 0.0099 0.0583 0.0103 0.0249 0.0296 0.0419 0.0257 0.0272 0.0322 0.0303 0.0258
Vacuole 0.0039 0.0016 0.002 0.0005 0.0018 0.0011 0.0096 0.0014 0.0012 0.0012 0.002 0.0006 0.0048 0.0011 0.0012 0.004 0.0021 0.001
Vacuole Periphery 0.0017 0.0005 0.0009 0.0002 0.0012 0.0004 0.0029 0.0003 0.0005 0.0008 0.0012 0.0005 0.0017 0.0002 0.0003 0.0007 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.387 17.9341 11.5335 12.4965 13.8726 16.0406 16.3198 17.1341 13.6288 12.6569
Translational Efficiency 0.5445 0.6102 1.0399 0.9068 0.7107 0.8998 0.7413 0.927 0.9885 0.8347

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Slx8

Slx8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slx8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available