Standard name
Human Ortholog
Description Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0.05 0.08
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.13 0.15 0.17
Bud Neck 0 0 0 0 0 0 0 – 0.06 0 0 0.05 0 0 0 0 0 0 0.11 0.15 0.09 0.1 0.1 0.14
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.88 0.91 0.97 0.96 0.93 0.9 – 0.77 0.82 0.68 0.77 0.95 0.98 0.99 0.93 0.94 0.93 0.53 0.59 0.6 0.68 0.57 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.07 – 0.22 0.18 0.42 0.29 0 0 0 0 0 0 0.08 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 19 8 0 4 0 6 – 0 1 3 2 0 0 0 1 1 1 13 20 11 0 3 11
Bud 6 9 2 0 0 1 4 – 6 3 7 3 0 0 1 0 0 0 15 28 11 5 10 25
Bud Neck 3 1 9 2 11 2 13 – 18 10 6 15 1 2 1 1 2 2 24 54 22 4 7 21
Bud Site 7 13 1 0 0 2 5 – 1 2 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 8 2 4 0 4 – 2 3 2 2 0 0 1 9 11 11 1 2 1 0 0 0
Cytoplasm 166 286 210 181 285 301 364 – 227 234 218 221 104 188 223 220 271 281 117 209 146 30 41 77
Endoplasmic Reticulum 2 2 2 1 0 0 1 – 0 0 1 0 3 4 0 15 10 9 1 0 0 0 0 0
Endosome 0 1 0 1 0 0 0 – 2 0 0 0 0 0 0 3 4 5 5 5 2 0 0 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 2 0 1 0 0 0 0
Mitochondria 7 3 0 2 0 20 27 – 65 52 134 84 0 1 0 2 1 2 17 9 24 0 1 0
Nucleus 1 2 1 0 1 1 1 – 3 4 5 1 0 0 0 2 2 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 1 0 0 0 – 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 5 4 0 0 0 4 0 – 0 1 4 1 0 0 1 0 1 2 0 0 0 0 0 0
SpindlePole 4 11 10 1 10 8 14 – 17 9 11 15 1 1 0 4 5 4 5 11 8 0 0 4
Vac/Vac Membrane 1 3 1 0 1 2 1 – 0 3 2 0 0 0 0 7 4 12 13 5 5 0 3 2
Unique Cell Count 194 325 232 187 297 323 404 294 285 319 287 109 192 225 237 287 302 222 355 246 44 73 148
Labelled Cell Count 216 355 254 191 316 341 440 342 322 397 345 110 196 227 265 313 333 222 355 246 44 73 148


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.9 5.3 4.8 5.0 4.3 4.1 – 3.6 3.9 3.2 3.4 5.0 5.1 4.8 7.0 6.8 6.5 4.7 5.3 6.3
Std Deviation (1e-4) 0.8 1.0 0.8 1.1 1.3 1.4 1.0 – 1.1 1.4 1.2 0.9 1.1 1.1 0.9 1.8 1.7 1.6 1.2 1.2 2.3
Intensity Change (Log2) – – – -0.14 -0.07 -0.31 -0.35 – -0.54 -0.44 -0.71 -0.63 -0.06 -0.06 -0.13 0.41 0.36 0.29 -0.16 0.02 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.278 -0.447 -0.7374 -0.8442 -1.0115 -0.3536 1.0313 -0.1357 -0.0892 -0.1881 0.3303 0.3549 0.9546 -0.9561 -0.7285 0.7114 -1.3934 -0.3473
Actin 0.0184 0.001 0.0107 0.0003 0.0044 0.0019 0.0357 0.0024 0.0166 0.0042 0.0206 0.0062 0.0761 0.0081 0.0404 0.0164 0 0.0195
Bud 0.0014 0.0043 0.001 0.0008 0.0003 0.0012 0.0053 0.0033 0.0045 0.0007 0.0015 0.0013 0.0012 0.0145 0.0042 0.0013 0 0.0068
Bud Neck 0.0674 0.0012 0.0004 0.0001 0.0002 0.3318 0.0335 0.0038 0.001 0.0014 0.0078 0.3232 0.1074 0.0012 0.0097 0.0008 0 0.2417
Bud Periphery 0.0027 0.0075 0.0021 0.0018 0.0006 0.0022 0.009 0.0033 0.0046 0.0012 0.003 0.0029 0.0016 0.0106 0.0037 0.0018 0 0.0024
Bud Site 0.06 0.0675 0.0049 0.0001 0.0003 0.0067 0.0613 0.0471 0.0084 0.0131 0.0139 0.0087 0.0649 0.0701 0.0129 0.0043 0 0.0028
Cell Periphery 0.0009 0.0006 0.0001 0 0 0.0002 0.0013 0.0006 0.0002 0.0003 0.0004 0.0004 0.0014 0.0007 0.0004 0.0008 0 0.0004
Cytoplasm 0.0711 0.0323 0.0189 0.022 0.0295 0.0377 0.0699 0.0255 0.0188 0.0133 0.0224 0.0437 0.0941 0.0644 0.0508 0.1109 0.0178 0.0984
Cytoplasmic Foci 0.0118 0.0053 0.007 0.0082 0.0127 0.0316 0.0274 0.0044 0.0038 0.006 0.0178 0.0128 0.0285 0.0066 0.0143 0.0106 0.0015 0.025
Eisosomes 0.0006 0.0002 0.0003 0.0001 0.0001 0.0001 0.0007 0.0002 0.0003 0.0002 0.0004 0.0002 0.0005 0.0002 0.0008 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0008 0.0004 0.0004 0.0004 0.0013 0.0005 0.0075 0.0004 0.0007 0.0003 0.0039 0.0022 0.0045 0.0009 0.0008 0.0207 0.0002 0.0029
Endosome 0.0029 0.0005 0.0011 0.0003 0.0113 0.0043 0.0242 0.0016 0.0016 0.0055 0.045 0.0063 0.0198 0.0007 0.004 0.0099 0.0001 0.0096
Golgi 0.0014 0.0001 0.0008 0.0001 0.0013 0.0005 0.0075 0.0005 0.0026 0.0054 0.0229 0.0022 0.0147 0.0002 0.0028 0.0019 0 0.0029
Lipid Particles 0.0045 0.0004 0.0013 0.0003 0.0023 0.0007 0.0177 0.0013 0.0012 0.0063 0.0209 0.0013 0.037 0.0003 0.005 0.004 0.0001 0.0018
Mitochondria 0.0088 0.0003 0.0006 0.0004 0.0023 0.0008 0.012 0.0044 0.009 0.0403 0.0125 0.0059 0.0134 0.0008 0.0012 0.0017 0.0001 0.0013
None 0.72 0.8753 0.9415 0.9584 0.9083 0.5602 0.6224 0.8868 0.9187 0.88 0.7648 0.565 0.4893 0.8136 0.8305 0.7894 0.9797 0.5526
Nuclear Periphery 0.0012 0.0003 0.0007 0.0005 0.0141 0.0016 0.01 0.0003 0.0008 0.0002 0.0101 0.0027 0.0077 0.0003 0.0007 0.0092 0.0001 0.0114
Nucleolus 0.0015 0.0003 0.0005 0.0003 0.0006 0.0007 0.0019 0.0047 0.0007 0.0068 0.0036 0.0015 0.0023 0.0007 0.0011 0.0006 0 0.0015
Nucleus 0.004 0.0008 0.001 0.0006 0.0024 0.0044 0.0059 0.0036 0.0021 0.0063 0.0026 0.0033 0.0038 0.0018 0.002 0.0047 0.0001 0.0086
Peroxisomes 0.0175 0.0003 0.0045 0.0005 0.0017 0.0071 0.0314 0.0027 0.0014 0.0049 0.0103 0.0023 0.0181 0.0007 0.0064 0.0009 0 0.0034
Punctate Nuclear 0.0014 0.0011 0.0017 0.0045 0.004 0.0044 0.0079 0.0026 0.0026 0.0019 0.0082 0.0064 0.0108 0.0027 0.0076 0.006 0.0001 0.0054
Vacuole 0.0013 0.0004 0.0003 0.0003 0.0009 0.0009 0.0057 0.0005 0.0004 0.0014 0.0049 0.001 0.0022 0.0008 0.0005 0.0032 0.0001 0.0011
Vacuole Periphery 0.0004 0 0.0001 0.0001 0.0012 0.0002 0.0017 0.0001 0.0001 0.0003 0.0025 0.0004 0.0009 0.0001 0.0001 0.0008 0 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6892 5.1553 6.8008 24.4086 95.1249 16.6508 7.8187 8.5998 14.7933 130.0658
Translational Efficiency 0.3886 0.6715 0.3201 0.6452 0.8058 0.4774 0.4089 0.4293 0.7433 0.8579

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (27%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dse1

Dse1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dse1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available