Standard name | |
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Human Ortholog | |
Description | Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | – | 1 | 3 | 3 | 3 | 17 | 13 | 2 | 13 | 3 | 12 | 1 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | – | 2 | 1 | 4 | 3 | 5 | 1 | 6 | 6 | 3 | 5 | 1 | 3 | 2 | 5 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 285 | – | 119 | 196 | 325 | 333 | 530 | 586 | 266 | 327 | 192 | 280 | 181 | 303 | 365 | 131 | 91 | 130 | 277 | 46 | 129 | 84 | 236 | 255 |
Endoplasmic Reticulum | 3 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 9 | 6 | 5 | 2 | 0 | 0 | 0 | 0 | 3 |
Endosome | 0 | – | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | – | 14 | 24 | 25 | 53 | 116 | 171 | 107 | 135 | 88 | 171 | 0 | 0 | 5 | 6 | 1 | 2 | 2 | 0 | 9 | 0 | 1 | 3 |
Nucleus | 0 | – | 0 | 1 | 0 | 1 | 2 | 2 | 2 | 0 | 1 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | – | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 5 | 0 | 3 | 1 | 1 | 9 | 2 | 5 | 5 |
Unique Cell Count | 290 | 126 | 204 | 331 | 346 | 565 | 658 | 305 | 389 | 230 | 363 | 185 | 304 | 368 | 138 | 93 | 136 | 293 | 48 | 152 | 88 | 255 | 280 | |
Labelled Cell Count | 294 | 137 | 226 | 359 | 395 | 678 | 787 | 390 | 486 | 292 | 475 | 187 | 311 | 384 | 159 | 104 | 148 | 293 | 48 | 152 | 88 | 255 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | – | 3.7 | 3.6 | 3.7 | 3.5 | 3.2 | 3.3 | 3.0 | 3.1 | 3.2 | 2.9 | 5.5 | 5.4 | 4.7 | 7.0 | 6.7 | 7.2 | 5.2 | 5.2 | 5.9 |
Std Deviation (1e-4) | 0.6 | – | 0.4 | 0.6 | 0.9 | 0.9 | 0.7 | 1.0 | 0.8 | 0.9 | 0.9 | 0.8 | 1.0 | 0.9 | 1.4 | 7.9 | 1.4 | 1.6 | 1.0 | 1.1 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.02 | -0.08 | -0.19 | -0.14 | -0.28 | -0.26 | -0.22 | -0.36 | 0.59 | 0.55 | 0.36 | 0.93 | 0.87 | 0.97 | 0.49 | 0.49 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 2.2 | 0.9 | -0.3 | -1.8 | -2.2 | -3.0 | -3.0 | -4.3 | 1.6 | 3.7 | 3.3 | 0.2 | 1.3 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.6 | 0 | 0 | -2.1 | -2.9 | -3.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5883 | 1.683 | 1.0951 | 0.4744 | 0.5175 | 1.205 | 2.8285 | 2.4825 | 2.4007 | 1.7657 | 2.4303 | 2.6699 | 0.0133 | 0.3988 | 0.4719 | 0.3286 | -0.0867 | 0.4478 |
Actin | 0.0005 | 0.0004 | 0.0009 | 0.0007 | 0.0003 | 0.0004 | 0.0664 | 0.0023 | 0.0021 | 0.0001 | 0.0144 | 0.016 | 0.0257 | 0.0019 | 0.0188 | 0.0001 | 0.001 | 0.0027 |
Bud | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0013 | 0.0003 | 0.002 | 0.0002 | 0.001 | 0.0006 | 0.0001 | 0.0099 | 0.0082 | 0.0002 | 0.0049 | 0.0022 |
Bud Neck | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0025 | 0.0054 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0.0012 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0015 | 0.0003 |
Bud Periphery | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.002 | 0.0003 | 0.0017 | 0.0001 | 0.0013 | 0.0008 | 0.0002 | 0.0018 | 0.0114 | 0.0001 | 0.0024 | 0.0014 |
Bud Site | 0.0002 | 0.001 | 0.0013 | 0.0008 | 0.0002 | 0.0001 | 0.0082 | 0.0114 | 0.0073 | 0.0001 | 0.004 | 0.001 | 0.0004 | 0.0059 | 0.0036 | 0.0001 | 0.0097 | 0 |
Cell Periphery | 0.0006 | 0.0013 | 0.0009 | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.6069 | 0.7212 | 0.7238 | 0.757 | 0.648 | 0.8487 | 0.4489 | 0.791 | 0.7403 | 0.8793 | 0.6437 | 0.812 | 0.5085 | 0.5952 | 0.4642 | 0.6661 | 0.6931 | 0.6435 |
Cytoplasmic Foci | 0.0038 | 0.006 | 0.0092 | 0.0094 | 0.0059 | 0.0059 | 0.0332 | 0.0042 | 0.0181 | 0.0075 | 0.0298 | 0.0108 | 0.0051 | 0.0115 | 0.0217 | 0.0059 | 0.0259 | 0.0073 |
Eisosomes | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0015 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0063 | 0.0043 | 0.0056 | 0.0029 | 0.0035 | 0.002 | 0.0146 | 0.0019 | 0.0023 | 0.003 | 0.0023 | 0.0033 | 0.0063 | 0.0015 | 0.0032 | 0.0009 | 0.0022 | 0.002 |
Endosome | 0.0022 | 0.0032 | 0.0073 | 0.0115 | 0.0054 | 0.0052 | 0.0431 | 0.0031 | 0.0049 | 0.0101 | 0.0535 | 0.0066 | 0.0053 | 0.0028 | 0.0077 | 0.0027 | 0.0133 | 0.0015 |
Golgi | 0.0002 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.013 | 0.0037 | 0.0035 | 0.0003 | 0.0227 | 0.0056 | 0.0018 | 0.0046 | 0.0018 | 0.0001 | 0.0019 | 0.0002 |
Lipid Particles | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0186 | 0.0006 | 0.0045 | 0.0001 | 0.0429 | 0.0095 | 0.0011 | 0.0005 | 0.0023 | 0.0001 | 0.0018 | 0.0003 |
Mitochondria | 0.0001 | 0.0169 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0118 | 0.009 | 0.0006 | 0.0002 | 0.0185 | 0.0157 | 0.0009 | 0.0039 | 0.0054 | 0.0001 | 0.0012 | 0.0002 |
None | 0.3751 | 0.2397 | 0.2422 | 0.2078 | 0.3295 | 0.1317 | 0.2719 | 0.1681 | 0.2027 | 0.0916 | 0.1155 | 0.0921 | 0.4369 | 0.3417 | 0.4374 | 0.3208 | 0.2232 | 0.3354 |
Nuclear Periphery | 0.0006 | 0.0003 | 0.0009 | 0.0006 | 0.0007 | 0.0003 | 0.0123 | 0.0003 | 0.0007 | 0.0007 | 0.0035 | 0.0023 | 0.0033 | 0.0003 | 0.001 | 0.0003 | 0.001 | 0.0004 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0 | 0.0018 | 0 |
Nucleus | 0.0005 | 0.0006 | 0.0011 | 0.001 | 0.0011 | 0.0006 | 0.0089 | 0.0005 | 0.0012 | 0.0009 | 0.0014 | 0.001 | 0.0005 | 0.0016 | 0.001 | 0.0004 | 0.0036 | 0.0004 |
Peroxisomes | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0195 | 0.001 | 0.004 | 0 | 0.0183 | 0.017 | 0.0004 | 0.0113 | 0.0045 | 0 | 0.0013 | 0.0006 |
Punctate Nuclear | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0105 | 0.0002 | 0.0011 | 0.0002 | 0.0008 | 0.0024 | 0.0004 | 0.003 | 0.0027 | 0.0001 | 0.0056 | 0.0003 |
Vacuole | 0.0013 | 0.0026 | 0.0037 | 0.0045 | 0.0034 | 0.0011 | 0.0056 | 0.0013 | 0.0019 | 0.0045 | 0.0186 | 0.001 | 0.0017 | 0.0015 | 0.0026 | 0.0017 | 0.004 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0016 | 0.0002 | 0.0002 | 0.0008 | 0.0061 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.077 | 17.2594 | 17.9301 | 21.0318 | 20.0261 | 13.8291 | 21.8864 | 22.1824 | 18.9055 | 23.4376 |
Translational Efficiency | 0.3403 | 0.4214 | 0.3568 | 0.2758 | 0.2834 | 0.4742 | 0.3313 | 0.3138 | 0.2791 | 0.2629 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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