Standard name | |
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Human Ortholog | |
Description | Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 4 | 1 | 4 | 7 | 4 | 2 | 1 | 4 | 9 | 4 |
Bud | 2 | 0 | 1 | 0 | 3 | 2 | 0 | 2 | 3 | 5 | 4 | 4 | 0 | 0 | 1 | 1 | 1 | 3 | 0 | 0 | 2 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 3 | 1 | 2 | 1 | 7 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 5 | 2 | 4 | 6 | 3 | 8 | 7 | 6 | 8 | 5 | 4 | 1 | 1 | 3 | 2 | 5 | 0 | 0 | 2 | 2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 4 | 1 | 0 |
Golgi | 0 | 3 | 2 | 2 | 6 | 1 | 1 | 2 | 0 | 0 | 2 | 2 | 2 | 15 | 17 | 4 | 22 | 21 | 4 | 16 | 8 | 6 | 16 | 14 |
Mitochondria | 327 | 110 | 101 | 93 | 135 | 178 | 200 | 271 | 208 | 222 | 166 | 212 | 98 | 201 | 203 | 4 | 7 | 5 | 309 | 87 | 88 | 58 | 119 | 134 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 1 | 6 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 8 | 4 | 4 | 0 | 5 | 15 | 24 | 22 | 17 | 11 | 18 | 11 | 3 | 4 | 5 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 0 | 1 |
Nucleolus | 36 | 5 | 10 | 11 | 12 | 16 | 46 | 44 | 32 | 34 | 30 | 39 | 1 | 5 | 7 | 6 | 3 | 3 | 1 | 1 | 1 | 1 | 1 | 2 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 4 | 3 | 7 | 1 | 4 | 3 | 4 | 4 |
SpindlePole | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 |
Vac/Vac Membrane | 11 | 9 | 5 | 1 | 15 | 10 | 10 | 9 | 0 | 9 | 6 | 3 | 8 | 11 | 25 | 4 | 8 | 4 | 4 | 7 | 1 | 3 | 5 | 6 |
Unique Cell Count | 333 | 118 | 112 | 96 | 152 | 194 | 226 | 301 | 225 | 244 | 190 | 229 | 104 | 207 | 232 | 34 | 53 | 50 | 337 | 122 | 117 | 99 | 161 | 177 |
Labelled Cell Count | 389 | 135 | 131 | 110 | 183 | 232 | 292 | 365 | 271 | 296 | 240 | 285 | 118 | 244 | 268 | 39 | 60 | 60 | 337 | 122 | 117 | 99 | 161 | 177 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 18.0 | 14.9 | 14.4 | 16.9 | 16.9 | 15.4 | 15.7 | 16.2 | 15.5 | 14.3 | 15.8 | 18.8 | 19.6 | 21.2 | 17.3 | 20.5 | 20.8 | 11.7 | 17.0 | 20.4 |
Std Deviation (1e-4) | 3.4 | 5.0 | 4.0 | 3.8 | 4.3 | 4.4 | 4.5 | 4.9 | 5.1 | 4.8 | 4.9 | 4.9 | 4.5 | 4.2 | 5.7 | 8.0 | 7.5 | 7.0 | 6.1 | 3.6 | 5.1 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.18 | 0.19 | 0.05 | 0.08 | 0.12 | 0.06 | -0.05 | 0.09 | 0.34 | 0.4 | 0.51 | 0.22 | 0.47 | 0.48 | -0.35 | 0.19 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.1 | 0 | 6.8 | 6.8 |
Mitochondria | 1.9 | -0.4 | 0.5 | -0.5 | 0 | 0.7 | 0.2 | -0.7 | 0.8 | 1.1 | 2.6 | -0.7 | -8.9 | -9.7 | -9.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.5 | 2.2 | 1.4 | 1.4 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.6 | -0.3 | -0.2 | 2.7 | 1.5 | 1.4 | 1.3 | 1.7 | 2.0 | -2.7 | -2.6 | -2.4 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.6 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0.3 | 1.9 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.748 | 17.2575 | 16.5092 | 15.0206 | 15.7633 | 16.4676 | 12.6824 | 14.2727 | 12.9909 | 13.0194 | 12.3184 | 13.3314 | 14.539 | 16.5562 | 16.5887 | 13.918 | 16.9507 | 16.7285 |
Actin | 0.014 | 0.0019 | 0.002 | 0.0001 | 0.0003 | 0.0029 | 0.0465 | 0.0027 | 0.0136 | 0.0025 | 0.0218 | 0.0044 | 0.0246 | 0.0269 | 0.0171 | 0.0146 | 0.0002 | 0.0075 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.0004 | 0.0004 | 0 | 0.0001 |
Bud Periphery | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0005 | 0.0038 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0001 |
Bud Site | 0.0011 | 0.0001 | 0.0054 | 0 | 0.0001 | 0.0001 | 0.0054 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0 | 0.0044 | 0.0003 | 0.004 | 0.0035 | 0 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0028 | 0.0004 | 0.001 | 0.0036 | 0 | 0.0003 |
Cytoplasmic Foci | 0.0083 | 0.0005 | 0.0012 | 0 | 0.0018 | 0.0008 | 0.0081 | 0.0011 | 0.003 | 0.0002 | 0.0011 | 0.0002 | 0.0337 | 0.0008 | 0.0098 | 0.0597 | 0 | 0.0024 |
Eisosomes | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0005 | 0.0009 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | 0.0004 | 0.0008 | 0.0013 | 0.0004 | 0.0009 | 0.0011 | 0 | 0.0002 |
Endosome | 0.0062 | 0.002 | 0.0044 | 0.0001 | 0.0019 | 0.003 | 0.0115 | 0.002 | 0.0023 | 0.0003 | 0.0024 | 0.0021 | 0.0223 | 0.0019 | 0.0088 | 0.0161 | 0.0003 | 0.0011 |
Golgi | 0.0065 | 0.0049 | 0.0039 | 0.0003 | 0.0015 | 0.0027 | 0.0282 | 0.0114 | 0.0034 | 0.0009 | 0.005 | 0.004 | 0.0139 | 0.0035 | 0.0077 | 0.007 | 0.0001 | 0.0041 |
Lipid Particles | 0.0135 | 0.0089 | 0.0025 | 0.0003 | 0.0116 | 0.0022 | 0.0348 | 0.0029 | 0.0042 | 0.0012 | 0.0048 | 0.0085 | 0.028 | 0.0067 | 0.0137 | 0.0195 | 0.0003 | 0.0076 |
Mitochondria | 0.9253 | 0.9705 | 0.9698 | 0.9982 | 0.9628 | 0.9759 | 0.8383 | 0.9649 | 0.9462 | 0.9865 | 0.9582 | 0.9646 | 0.8257 | 0.9372 | 0.9159 | 0.7765 | 0.9843 | 0.9632 |
None | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.004 | 0.0032 | 0.0005 | 0.0027 | 0 | 0.0065 |
Nuclear Periphery | 0.001 | 0 | 0 | 0 | 0.0002 | 0.0003 | 0.0007 | 0 | 0.0019 | 0 | 0.0002 | 0.0063 | 0.0018 | 0.0015 | 0.0012 | 0.002 | 0 | 0.0004 |
Nucleolus | 0.0032 | 0.0002 | 0.0003 | 0 | 0.0108 | 0 | 0.0024 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0007 | 0.0005 | 0.0007 | 0 | 0.0002 |
Nucleus | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.001 | 0.0007 | 0.0009 | 0.0005 | 0.0024 | 0 | 0.0001 |
Peroxisomes | 0.0048 | 0.0024 | 0.002 | 0.0004 | 0.0013 | 0.0019 | 0.0127 | 0.0035 | 0.0095 | 0.0025 | 0.0027 | 0.0006 | 0.0217 | 0.0053 | 0.006 | 0.0387 | 0.0005 | 0.0016 |
Punctate Nuclear | 0.0014 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0033 | 0 | 0.0001 | 0.0001 | 0.0059 | 0.0041 | 0.0007 | 0.0068 | 0 | 0.0005 |
Vacuole | 0.0025 | 0.0023 | 0.0027 | 0.0001 | 0.0019 | 0.0011 | 0.0012 | 0.0038 | 0.0018 | 0.0002 | 0.0002 | 0.0006 | 0.002 | 0.0013 | 0.003 | 0.0086 | 0.0043 | 0.0003 |
Vacuole Periphery | 0.0091 | 0.0053 | 0.0038 | 0.0005 | 0.0041 | 0.0073 | 0.0039 | 0.0061 | 0.004 | 0.0005 | 0.0007 | 0.0059 | 0.0041 | 0.0034 | 0.0067 | 0.0348 | 0.0094 | 0.0032 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.6781 | 22.2795 | 23.5461 | 35.0453 | 34.7616 | 41.5152 | 36.5208 | 46.3034 | 53.0296 | 41.1561 |
Translational Efficiency | 1.2779 | 1.6535 | 1.1941 | 1.2923 | 1.0968 | 0.9927 | 1.2796 | 0.9689 | 0.9081 | 0.9514 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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