ORF | |
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Human Ortholog | |
Description | Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 4 | 7 | 11 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 5 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 1 | 1 | 2 | 6 | 10 | 2 | 5 | 0 | 4 | 0 | 0 | 0 | 7 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 291 | 114 | 299 | 222 | 326 | 327 | 402 | 401 | 319 | 340 | 130 | 213 | 20 | 189 | 239 | 277 | 258 | 187 | 279 | 117 | 284 | 62 | 213 | 172 |
Endoplasmic Reticulum | 2 | 0 | 2 | 0 | 1 | 0 | 3 | 3 | 9 | 4 | 1 | 7 | 0 | 0 | 2 | 8 | 10 | 6 | 1 | 0 | 3 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 6 | 1 | 3 | 13 | 14 | 63 | 138 | 127 | 136 | 202 | 93 | 131 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Nucleus | 2 | 1 | 1 | 2 | 3 | 3 | 16 | 18 | 16 | 13 | 4 | 11 | 0 | 1 | 4 | 11 | 7 | 2 | 1 | 2 | 0 | 2 | 1 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 3 | 5 | 13 | 27 | 29 | 53 | 86 | 77 | 115 | 26 | 30 | 0 | 2 | 0 | 35 | 16 | 12 | 4 | 0 | 4 | 0 | 2 | 1 |
Unique Cell Count | 296 | 118 | 307 | 236 | 354 | 386 | 545 | 584 | 486 | 565 | 211 | 345 | 20 | 190 | 243 | 304 | 276 | 198 | 300 | 125 | 315 | 66 | 227 | 185 |
Labelled Cell Count | 303 | 119 | 316 | 251 | 374 | 425 | 620 | 651 | 570 | 693 | 255 | 405 | 20 | 192 | 245 | 339 | 297 | 214 | 300 | 125 | 315 | 66 | 227 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 8.0 | 7.4 | 7.0 | 7.2 | 6.4 | 6.3 | 6.2 | 5.8 | 5.6 | 5.3 | 5.5 | 7.0 | 8.4 | 8.1 | 14.0 | 13.3 | 13.7 | 7.1 | 8.2 | 8.7 |
Std Deviation (1e-4) | 1.3 | 1.6 | 1.3 | 1.8 | 1.7 | 1.2 | 1.3 | 1.3 | 1.2 | 1.2 | 1.3 | 1.2 | 0.9 | 1.3 | 1.1 | 2.7 | 2.9 | 3.1 | 0.8 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.06 | -0.22 | -0.25 | -0.27 | -0.35 | -0.41 | -0.48 | -0.44 | -0.09 | 0.18 | 0.12 | 0.91 | 0.84 | 0.88 | -0.06 | 0.14 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 |
Cytoplasm | -1.9 | -3.0 | -5.6 | -8.7 | -9.9 | -10.5 | -11.9 | -10.6 | -11.1 | 0.7 | 1.7 | 0.8 | -3.3 | -2.3 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 2.4 | 6.8 | 9.2 | 8.3 | 9.7 | 11.6 | 12.4 | 11.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.6 | 2.7 | 2.8 | 0 | 0 | 2.7 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.5 | 3.6 | 3.6 | 4.5 | 6.1 | 6.4 | 7.7 | 5.0 | 4.0 | 0 | 0 | 0 | 4.9 | 2.7 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6935 | 3.2694 | 3.2958 | 2.5324 | 2.3646 | 3.0306 | 2.2458 | 2.2398 | 1.8574 | 1.9744 | 1.5912 | 2.0078 | 1.2089 | 2.0489 | 1.1524 | 1.2097 | 1.4162 | 1.2415 |
Actin | 0.008 | 0.0005 | 0.0017 | 0.0004 | 0.0003 | 0.0022 | 0.0471 | 0.001 | 0.0159 | 0.0065 | 0.0052 | 0.0076 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0005 | 0.0004 |
Bud | 0.0002 | 0.0042 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0033 | 0.0008 | 0.0011 | 0.0009 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0014 | 0.0004 | 0.0013 | 0.0004 | 0.0003 | 0.0009 | 0.0147 | 0.0003 | 0.0009 | 0.0013 | 0.0006 | 0.0052 | 0.0001 | 0.0001 | 0.0014 | 0.0005 | 0.0005 | 0.0044 |
Bud Periphery | 0.0003 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0047 | 0.0004 | 0.0011 | 0.0006 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Site | 0.0009 | 0.0107 | 0.0022 | 0.0001 | 0.0001 | 0.0002 | 0.0089 | 0.0065 | 0.0037 | 0.0056 | 0.0006 | 0.0004 | 0.0001 | 0.0005 | 0.0011 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6392 | 0.7646 | 0.7403 | 0.7305 | 0.8085 | 0.759 | 0.5479 | 0.8676 | 0.8039 | 0.8259 | 0.8505 | 0.8644 | 0.8111 | 0.8914 | 0.8671 | 0.8702 | 0.8732 | 0.8458 |
Cytoplasmic Foci | 0.0096 | 0.0066 | 0.0051 | 0.0096 | 0.0077 | 0.008 | 0.0354 | 0.0092 | 0.017 | 0.0283 | 0.0154 | 0.0165 | 0.005 | 0.0036 | 0.005 | 0.0079 | 0.0226 | 0.0072 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0018 | 0.002 | 0.0015 | 0.0024 | 0.0019 | 0.0092 | 0.0023 | 0.0057 | 0.0042 | 0.0033 | 0.0046 | 0.0043 | 0.0029 | 0.0022 | 0.0021 | 0.0032 | 0.002 |
Endosome | 0.0029 | 0.0017 | 0.0017 | 0.0032 | 0.0036 | 0.0047 | 0.0554 | 0.0031 | 0.0127 | 0.0238 | 0.0116 | 0.0186 | 0.0056 | 0.0021 | 0.0022 | 0.0072 | 0.0091 | 0.0064 |
Golgi | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0155 | 0.0005 | 0.0025 | 0.0078 | 0.003 | 0.0056 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0007 |
Lipid Particles | 0.0018 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0105 | 0.0015 | 0.0023 | 0.0082 | 0.015 | 0.004 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0034 |
Mitochondria | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0158 | 0.0006 | 0.0034 | 0.0044 | 0.0023 | 0.002 | 0.0044 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0008 |
None | 0.3185 | 0.1997 | 0.2354 | 0.2435 | 0.1678 | 0.2131 | 0.1444 | 0.0917 | 0.1003 | 0.0549 | 0.0427 | 0.0448 | 0.1466 | 0.0895 | 0.1014 | 0.0781 | 0.0622 | 0.1058 |
Nuclear Periphery | 0.0012 | 0.0006 | 0.0009 | 0.0007 | 0.0013 | 0.0009 | 0.0315 | 0.0014 | 0.008 | 0.0028 | 0.0112 | 0.0039 | 0.003 | 0.0017 | 0.0015 | 0.0027 | 0.0023 | 0.0017 |
Nucleolus | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0015 | 0.0001 | 0.0006 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.001 |
Nucleus | 0.0041 | 0.0039 | 0.0046 | 0.0067 | 0.005 | 0.0034 | 0.0206 | 0.0044 | 0.0098 | 0.0093 | 0.0186 | 0.0128 | 0.0128 | 0.0057 | 0.0141 | 0.0262 | 0.0158 | 0.016 |
Peroxisomes | 0.0029 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0002 | 0.0089 | 0.005 | 0.0021 | 0.0051 | 0.01 | 0.0029 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0006 |
Punctate Nuclear | 0.0019 | 0.001 | 0.0009 | 0.001 | 0.0011 | 0.0031 | 0.0104 | 0.0022 | 0.0054 | 0.0066 | 0.0038 | 0.0027 | 0.003 | 0.0006 | 0.0013 | 0.001 | 0.0068 | 0.0026 |
Vacuole | 0.0007 | 0.001 | 0.0006 | 0.0007 | 0.0007 | 0.0007 | 0.0082 | 0.0013 | 0.0024 | 0.0024 | 0.003 | 0.0022 | 0.0011 | 0.0009 | 0.0009 | 0.002 | 0.002 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0054 | 0.0002 | 0.0008 | 0.0007 | 0.0011 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.2887 | 37.7667 | 24.7275 | 35.9834 | 58.5348 | 37.2565 | 35.378 | 31.5844 | 39.964 | 62.0977 |
Translational Efficiency | 0.4464 | 0.5098 | 0.4622 | 0.4871 | 0.4933 | 0.5914 | 0.5057 | 0.724 | 0.454 | 0.4483 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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