Standard name
Human Ortholog
Description Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.99 0.94 0.97 1.0 0.88 0.94 – 0.75 0.82 0.68 0.69 1.0 0.99 1.0 0.83 0.81 0.86 0.86 0.92 0.93 0.9 0.85 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0.21 0.08 – 0.51 0.28 0.51 0.43 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0 0 0 – 0.09 0 0 0 0 0 0 0.21 0.19 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 0 3 2
Bud 0 1 0 0 0 3 0 – 1 2 3 4 0 0 0 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 – 1 1 1 2 2 0 1 4 1 2 1 0 0 0 0 0
Cytoplasm 148 234 16 36 111 94 150 – 76 190 100 135 228 295 337 66 35 56 141 220 38 114 197 135
Endoplasmic Reticulum 2 0 0 0 0 1 0 – 0 0 0 1 0 1 0 6 0 2 0 0 0 0 2 2
Endosome 1 1 0 0 0 0 2 – 0 0 3 0 0 0 0 1 1 0 5 2 0 1 3 0
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 1 0 2 2 0
Mitochondria 7 2 0 3 3 23 12 – 52 66 74 84 0 1 0 4 2 1 1 4 1 2 5 5
Nucleus 1 0 1 0 1 2 1 – 2 0 1 5 0 0 0 1 2 2 0 0 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 0 – 1 1 0 2 0 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 – 0 3 1 0 0 0 0 0 0 0 1 0 0 1 3 2
Vac/Vac Membrane 2 1 0 3 2 1 4 – 9 4 2 2 1 0 1 17 8 11 0 3 0 1 2 0
Unique Cell Count 159 236 17 37 111 107 159 102 233 146 196 228 297 337 80 43 65 164 239 41 128 232 163
Labelled Cell Count 163 240 17 42 117 125 170 142 269 185 235 231 298 339 99 50 74 164 239 41 128 232 163


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.9 4.9 5.2 5.6 4.1 5.0 – 4.2 4.4 4.2 4.2 5.6 5.8 5.9 8.8 9.1 9.1 4.7 5.7 6.0
Std Deviation (1e-4) 1.4 0.9 0.6 1.6 1.4 1.7 2.0 – 1.3 1.7 1.5 1.4 1.2 1.3 1.3 1.6 2.7 1.6 1.5 2.3 2.2
Intensity Change (Log2) – – – 0.11 0.2 -0.23 0.06 – -0.22 -0.14 -0.22 -0.21 0.21 0.26 0.29 0.86 0.91 0.9 -0.04 0.23 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 2.6 -0.8 0 – -1.8 -1.3 -2.2 -2.2 3.7 2.1 4.5 -1.2 -1.2 -0.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7249 -0.4548 -0.608 -1.0914 -0.8584 -0.6204 0.6203 1.2773 1.0097 0.9873 0.234 1.0782 2.7502 3.5212 2.7498 2.9601 2.5574 3.1198
Actin 0.0439 0.0001 0.0057 0.0008 0.0059 0.0055 0.0126 0.0006 0.0079 0.0047 0.0045 0.0083 0.008 0.0014 0.0008 0.0002 0.0002 0.0002
Bud 0.0004 0.0002 0.0008 0.0001 0.0006 0.0003 0.0132 0.0127 0.006 0.0095 0.0058 0.0013 0.0001 0.002 0.0001 0 0 0.0001
Bud Neck 0.0014 0.0003 0.0005 0.0001 0.0003 0.0007 0.0027 0.0003 0.0022 0.0011 0.0002 0.0012 0.0009 0.0002 0.0001 0.0001 0.0001 0.0003
Bud Periphery 0.0005 0.0001 0.0026 0 0.001 0.0007 0.006 0.0083 0.0061 0.0027 0.0107 0.0015 0.0001 0.0016 0.0002 0 0 0
Bud Site 0.0027 0.0029 0.0022 0.0001 0.0006 0.0001 0.0176 0.0112 0.0087 0.0028 0.0005 0.0004 0.0004 0.0113 0.0004 0.0001 0 0
Cell Periphery 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0 0.0001 0.0001 0.0002 0.0001 0 0 0
Cytoplasm 0.2538 0.467 0.2984 0.3933 0.3513 0.4315 0.3616 0.5487 0.5216 0.5401 0.5576 0.6114 0.4089 0.6005 0.4425 0.4748 0.5194 0.5124
Cytoplasmic Foci 0.03 0.0182 0.0127 0.0258 0.0234 0.0123 0.0192 0.0057 0.0262 0.0171 0.0089 0.0188 0.0045 0.0049 0.0036 0.0041 0.003 0.0047
Eisosomes 0.0004 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0037 0.0032 0.0027 0.0023 0.0037 0.0041 0.0032 0.0012 0.0027 0.0014 0.0007 0.0014 0.0041 0.0044 0.0015 0.0019 0.0017 0.0011
Endosome 0.0243 0.0052 0.0057 0.0029 0.0237 0.0042 0.0315 0.0038 0.0163 0.007 0.0025 0.0043 0.0039 0.0012 0.003 0.0017 0.0006 0.0007
Golgi 0.0076 0.0003 0.0013 0.0003 0.0041 0.0012 0.0054 0.0015 0.0047 0.0033 0.0021 0.0031 0.0006 0.0003 0.0008 0.0001 0.0001 0.0001
Lipid Particles 0.0114 0.0009 0.0031 0.0018 0.0072 0.0011 0.0099 0.0002 0.0012 0.0099 0.0004 0.0023 0.0007 0.0003 0.0026 0.0001 0 0.0001
Mitochondria 0.005 0.0002 0.0031 0.0003 0.0083 0.0007 0.0087 0.0035 0.0069 0.0143 0.0307 0.0055 0.0003 0.0002 0.0024 0.0001 0.0001 0.0002
None 0.59 0.4909 0.6359 0.5598 0.523 0.5282 0.4673 0.3862 0.3687 0.3416 0.3641 0.3225 0.5426 0.3678 0.5357 0.514 0.472 0.4761
Nuclear Periphery 0.0039 0.0011 0.0058 0.0014 0.0159 0.001 0.009 0.0008 0.0019 0.0026 0.0004 0.001 0.0074 0.0006 0.0006 0.0005 0.0003 0.0004
Nucleolus 0.0017 0.0002 0.0037 0.0001 0.001 0.0001 0.0018 0.0004 0.0008 0.0018 0.0001 0.0004 0.0005 0.0001 0.0001 0 0 0.0001
Nucleus 0.0034 0.0042 0.0039 0.0025 0.0088 0.0037 0.0066 0.0061 0.0051 0.014 0.0016 0.0064 0.0114 0.0015 0.0013 0.001 0.0018 0.002
Peroxisomes 0.0063 0.0001 0.0014 0.0012 0.0014 0.0003 0.0066 0.0003 0.0017 0.0084 0.0075 0.0028 0.0002 0.0001 0.0025 0 0.0001 0.0001
Punctate Nuclear 0.0044 0.0022 0.0073 0.006 0.0142 0.0032 0.0072 0.0057 0.0085 0.0151 0.0007 0.0065 0.0041 0.0006 0.0006 0.0004 0.0004 0.001
Vacuole 0.0041 0.0021 0.002 0.0009 0.0032 0.0008 0.0075 0.002 0.0019 0.0016 0.0005 0.0008 0.0008 0.0006 0.0006 0.0005 0.0002 0.0002
Vacuole Periphery 0.0008 0.0002 0.0008 0.0002 0.0023 0.0002 0.0017 0.0003 0.0006 0.0006 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.1495 31.0447 15.7067 33.8946 32.7465 33.1311 27.4656 30.8155 38.4543 35.2478
Translational Efficiency 0.7092 0.7134 1.4367 0.6709 0.6848 1.0057 0.8833 1.0122 0.954 1.0002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
Localization
Cell Percentages cytoplasm (38%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gcg1

Gcg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available