Standard name
Human Ortholog
Description Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.35 0.44 0.41 0.19 0.12 0.13 0.1 0.16 0.18 0.11 0.21 0.11 0.43 0.42 0.29 – – – 0.32 0.37 0.4 0.35 0.34 0.33
Bud 0.13 0.12 0.17 0.15 0.16 0.15 0.14 0.14 0.12 0.19 0.18 0.14 0.1 0.1 0.08 – – – 0.09 0.11 0.06 0.21 0.17 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.07 0.06 0.05 0.06 0 0
Bud Site 0 0.06 0.05 0 0 0.05 0 0 0 0 0.05 0.05 0 0 0 – – – – – – – – –
Cell Periphery 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0
Cytoplasm 0.11 0.14 0.08 0.08 0.12 0.1 0.1 0.14 0.09 0.11 0.13 0.13 0.14 0.13 0.13 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.15 0.22 0.11 0.09 0.07 0.13 0.1 0.07 0.1 0.06 0.14 0.06 0.35 0.33 0.35 – – – 0.08 0.07 0.06 0 0.08 0.09
Endosome 0.07 0 0.05 0.06 0.15 0 0.06 0.05 0 0 0 0.05 0 0 0 – – – 0.09 0.08 0.1 0.08 0.06 0.08
Golgi 0.14 0.1 0.07 0 0 0 0 0 0 0 0 0 0.13 0.15 0.23 – – – 0.15 0.15 0.14 0.07 0.13 0.15
Mitochondria 0.19 0.28 0.28 0.65 0.38 0.75 0.65 0.6 0.7 0.7 0.63 0.69 0 0 0 – – – 0.1 0.05 0.09 0.1 0.1 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.07 0 0.21 0.14 0.1 0.06 0.05 0.05 0 0 0 0 0.05 0.08 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 124 99 125 39 30 39 44 52 54 35 63 36 81 83 54 – – – 180 108 163 190 172 194
Bud 44 26 52 30 39 44 59 46 36 60 54 46 19 19 15 – – – 52 33 22 113 85 64
Bud Neck 2 1 1 0 0 0 1 1 0 1 0 0 0 0 0 – – – 41 17 20 34 14 14
Bud Site 10 13 14 7 8 14 17 10 11 12 14 15 0 0 0 – – – – – – – – –
Cell Periphery 19 6 14 1 3 3 8 5 12 11 13 7 6 6 9 – – – 4 2 0 0 2 2
Cytoplasm 39 31 25 17 28 30 44 44 28 33 37 41 26 26 25 – – – 4 4 3 1 2 6
Endoplasmic Reticulum 54 49 33 19 18 39 42 23 30 19 40 18 66 66 65 – – – 46 20 23 22 41 52
Endosome 25 10 16 13 37 5 26 17 10 8 6 16 5 4 5 – – – 51 23 40 41 30 49
Golgi 48 22 20 9 2 1 1 0 0 1 0 1 24 29 43 – – – 87 45 56 37 64 85
Mitochondria 66 63 84 133 92 224 276 194 209 220 185 220 4 4 1 – – – 56 15 34 52 51 64
Nucleus 0 0 4 1 1 0 2 4 1 2 0 1 3 3 0 – – – 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 2 0 0 1 0 0 0 – – – 3 3 1 2 1 2
Nucleolus 14 2 3 0 4 7 13 12 2 12 5 10 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 26 10 65 28 25 19 21 16 11 8 6 11 9 16 6 – – – 12 2 16 12 9 7
SpindlePole 2 1 2 0 8 2 5 4 3 5 7 9 9 9 6 – – – 1 0 1 1 2 3
Vac/Vac Membrane 19 5 11 4 16 8 11 10 2 4 6 3 6 7 8 – – – 24 12 15 27 25 28
Unique Cell Count 350 225 303 206 242 300 422 321 298 313 295 319 188 199 186 571 293 406 541 506 582
Labelled Cell Count 493 339 469 301 311 435 570 438 411 431 436 435 258 272 237 571 293 406 541 506 582


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 10.1 7.6 6.9 7.1 6.2 6.4 6.8 6.1 6.1 6.3 6.1 9.7 9.7 10.2 – – – 9.2 9.4 9.8
Std Deviation (1e-4) 1.4 1.8 1.4 1.4 1.7 1.2 1.4 1.5 1.3 1.2 1.5 1.3 1.9 1.9 2.2 – – – 1.4 1.4 1.6
Intensity Change (Log2) – – – -0.14 -0.1 -0.29 -0.25 -0.15 -0.3 -0.3 -0.27 -0.31 0.35 0.35 0.43 – – – 0.28 0.31 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -5.3 -7.4 -7.8 -9.7 -6.9 -6.2 -8.5 -5.2 -8.5 0.4 0.1 -2.7 – – –
Bud -0.8 -0.3 -0.8 -1.2 -1.0 -1.8 0.6 0.4 -0.9 -2.2 -2.4 -2.8 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site -0.7 -0.8 0 -0.4 -1.0 -0.6 -0.5 0.1 0 -3.0 -3.1 -3.0 – – –
Cell Periphery -2.7 -2.3 -2.7 -2.1 -2.2 -0.4 -0.7 -0.1 -1.7 -0.8 -0.9 0.1 – – –
Cytoplasm 0 1.3 0.7 1.0 2.2 0.5 1.0 1.7 1.9 2.0 1.7 1.8 – – –
Endoplasmic Reticulum -0.6 -1.4 0.8 -0.4 -1.6 -0.3 -2.2 1.0 -2.4 6.5 6.1 6.5 – – –
Endosome 0.5 3.9 -2.4 0.5 0 -1.2 -1.7 -2.1 -0.1 -1.4 -1.8 -1.4 – – –
Golgi -1.1 -3.4 -4.2 -5.0 -4.7 -4.5 -4.3 -4.5 -4.3 2.3 2.9 5.3 – – –
Mitochondria 8.2 2.6 11.5 10.0 8.2 10.4 10.6 8.6 10.3 -7.2 -7.4 -7.7 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 1.9 2.2 0 2.3 0 1.9 0 0 0 – – –
Peroxisomes -2.3 -3.5 -5.4 -6.8 -6.1 -6.6 -7.3 -7.3 -6.9 -5.0 -4.0 -5.6 – – –
SpindlePole 0 0 0 0 0 0 0 0 2.0 0 0 0 – – –
Vacuole -1.1 1.6 -0.7 -0.8 -0.4 -2.5 -1.9 -1.2 -2.3 -0.3 -0.1 0.4 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.495 5.353 5.2241 5.0998 4.6444 5.0687 7.1256 7.518 7.2157 6.9359 6.7067 7.4012 7.1981 8.1692 7.8318 8.2272 7.5211 8.2945
Actin 0.1937 0.0984 0.1522 0.1294 0.2068 0.16 0.2855 0.1846 0.2577 0.2551 0.2299 0.2248 0.2214 0.1317 0.2231 0.1815 0.1859 0.2224
Bud 0.0187 0.0249 0.0984 0.0291 0.0242 0.0049 0.0154 0.0126 0.0816 0.0509 0.0106 0.0035 0.0126 0.0107 0.0875 0.0282 0.0068 0.0035
Bud Neck 0.0181 0.0068 0.0032 0.0028 0.0312 0.2318 0.0328 0.0163 0.0043 0.0384 0.0068 0.1976 0.0277 0.0069 0.0059 0.0026 0.04 0.224
Bud Periphery 0.0247 0.0197 0.1405 0.0777 0.0544 0.0056 0.0266 0.0198 0.1279 0.1074 0.0245 0.0055 0.0172 0.0128 0.1041 0.1159 0.0147 0.0049
Bud Site 0.0691 0.1275 0.0323 0.0071 0.0086 0.0064 0.046 0.1857 0.0273 0.0157 0.0086 0.0053 0.0619 0.1226 0.0473 0.0044 0.0048 0.0063
Cell Periphery 0.0034 0.002 0.0014 0.0017 0.0027 0.002 0.008 0.0089 0.0038 0.0025 0.003 0.0108 0.0036 0.0014 0.0016 0.0025 0.001 0.0023
Cytoplasm 0.0614 0.0522 0.033 0.036 0.0671 0.0231 0.048 0.0828 0.0416 0.0247 0.0558 0.0521 0.0326 0.062 0.0275 0.0229 0.0449 0.032
Cytoplasmic Foci 0.0982 0.1303 0.0814 0.0894 0.0778 0.0856 0.1 0.1118 0.0717 0.06 0.1144 0.0893 0.0984 0.162 0.0791 0.0575 0.1076 0.0853
Eisosomes 0.0005 0.0003 0.0003 0.0004 0.0005 0.0005 0.0033 0.0031 0.0007 0.0005 0.0007 0.0006 0.0008 0.0003 0.0008 0.001 0.0006 0.0005
Endoplasmic Reticulum 0.0179 0.0033 0.0046 0.014 0.0176 0.0044 0.0109 0.0047 0.0027 0.0095 0.0026 0.0036 0.0056 0.0053 0.0028 0.0356 0.0062 0.0072
Endosome 0.1969 0.225 0.1801 0.2745 0.1729 0.1338 0.1153 0.137 0.1071 0.1246 0.1623 0.102 0.1656 0.2181 0.1435 0.1975 0.16 0.1025
Golgi 0.2053 0.2331 0.1982 0.2374 0.2387 0.2871 0.2159 0.1889 0.2059 0.2586 0.286 0.2525 0.2186 0.2002 0.1991 0.2733 0.3744 0.2456
Lipid Particles 0.0256 0.0078 0.0098 0.0172 0.007 0.01 0.0249 0.0053 0.0068 0.0036 0.0215 0.0061 0.0274 0.0088 0.0074 0.0047 0.01 0.0082
Mitochondria 0.0202 0.0309 0.0277 0.0398 0.0578 0.02 0.0249 0.0179 0.0316 0.026 0.0228 0.0184 0.0338 0.0144 0.0362 0.0355 0.0113 0.0289
None 0.0046 0.0008 0.0018 0.0014 0.0024 0.0012 0.0046 0.0019 0.0066 0.0006 0.001 0.0014 0.0036 0.0045 0.0038 0.0218 0.0044 0.0009
Nuclear Periphery 0.0073 0.0014 0.0014 0.0029 0.0094 0.004 0.0014 0.0007 0.0009 0.0014 0.0006 0.0032 0.0135 0.001 0.0009 0.0018 0.0033 0.0003
Nucleolus 0.0007 0.0005 0.0003 0.0002 0.0005 0.0002 0.0052 0.0002 0.0002 0.0003 0.0002 0.0002 0.0008 0.0005 0.0002 0.0001 0.0008 0.0002
Nucleus 0.0031 0.0012 0.0007 0.0007 0.0022 0.0006 0.0015 0.0013 0.0008 0.0006 0.0004 0.0007 0.006 0.0009 0.0005 0.0005 0.0019 0.0003
Peroxisomes 0.012 0.0111 0.0174 0.0228 0.0057 0.0117 0.019 0.0089 0.0091 0.0075 0.0409 0.0154 0.0305 0.0203 0.0171 0.003 0.0113 0.0186
Punctate Nuclear 0.0079 0.0021 0.0022 0.0018 0.0022 0.0009 0.0012 0.0006 0.0006 0.0004 0.0017 0.0023 0.0073 0.0011 0.0006 0.0004 0.0061 0.0005
Vacuole 0.0081 0.0176 0.0094 0.009 0.0065 0.0042 0.0075 0.0055 0.0085 0.0091 0.0038 0.0032 0.0084 0.0119 0.0074 0.0061 0.0026 0.0042
Vacuole Periphery 0.0026 0.0032 0.0037 0.0048 0.0037 0.0021 0.0019 0.0014 0.0029 0.0027 0.0019 0.0015 0.0026 0.0023 0.0035 0.0033 0.0013 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.8616 31.1641 22.5043 31.814 29.7286 26.8917 43.4056 43.462 36.7311 42.8649
Translational Efficiency 0.6024 0.7096 0.7494 0.4966 0.5565 0.665 0.5032 0.3909 0.4096 0.4073

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
Localization
Cell Percentages ER (16%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Dnf1

Dnf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dnf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available