Standard name | |
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Human Ortholog | |
Description | Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 3 | – | 1 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 0 | 4 | 4 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 53 | 70 | 3 | 39 | 69 | 97 | 142 | – | 81 | 96 | 64 | 91 | 75 | 140 | 184 | 121 | 135 | 125 | 38 | 45 | 21 | 27 | 103 | 112 |
Endoplasmic Reticulum | 1 | 0 | 3 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 11 | 14 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3 | 0 | 0 | 0 | 1 | 3 | – | 1 | 1 | 0 | 2 | 0 | 3 | 7 | 11 | 12 | 6 | 1 | 1 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 28 | 8 | 0 | 5 | 3 | 23 | 65 | – | 80 | 90 | 142 | 101 | 0 | 1 | 0 | 4 | 8 | 8 | 6 | 4 | 5 | 0 | 4 | 4 |
Nucleus | 21 | 59 | 14 | 15 | 18 | 25 | 53 | – | 32 | 46 | 31 | 52 | 39 | 38 | 21 | 30 | 33 | 25 | 63 | 92 | 12 | 11 | 34 | 38 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 2 | – | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 1 | 7 | 5 |
Vac/Vac Membrane | 47 | 81 | 0 | 8 | 29 | 25 | 36 | – | 16 | 32 | 9 | 9 | 15 | 32 | 36 | 117 | 128 | 85 | 47 | 51 | 25 | 16 | 45 | 44 |
Unique Cell Count | 122 | 186 | 17 | 66 | 112 | 155 | 260 | 177 | 229 | 203 | 221 | 123 | 193 | 240 | 247 | 285 | 223 | 167 | 206 | 72 | 60 | 211 | 220 | |
Labelled Cell Count | 150 | 221 | 21 | 70 | 122 | 173 | 305 | 212 | 277 | 263 | 260 | 133 | 218 | 251 | 298 | 335 | 256 | 167 | 206 | 72 | 60 | 211 | 220 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.6 | 4.6 | 5.0 | 5.8 | 4.6 | 4.4 | – | 3.9 | 4.0 | 3.4 | 3.8 | 5.2 | 5.3 | 5.2 | 6.2 | 6.1 | 5.8 | – | 5.0 | 5.1 |
Std Deviation (1e-4) | 0.5 | 1.0 | 0.3 | 1.1 | 2.4 | 1.3 | 1.4 | – | 1.1 | 1.2 | 0.8 | 1.1 | 0.9 | 1.0 | 1.2 | 1.5 | 1.5 | 1.5 | – | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.35 | -0.0 | -0.04 | – | -0.24 | -0.17 | -0.42 | -0.27 | 0.2 | 0.23 | 0.19 | 0.45 | 0.42 | 0.36 | – | 0.12 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.8 | 3.6 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0326 | -0.7805 | -0.8863 | -0.7216 | -0.6715 | -1.1075 | -0.5573 | -0.0132 | -0.2533 | -0.7371 | -0.4179 | -0.3997 | -1.6065 | -1.3105 | -1.5157 | -1.1021 | -1.3836 | -1.4694 |
Actin | 0.0215 | 0.0012 | 0.0129 | 0.0046 | 0.0128 | 0.0057 | 0.0374 | 0.0003 | 0.0031 | 0.0248 | 0.0116 | 0.0117 | 0.004 | 0.0003 | 0.003 | 0.01 | 0.0207 | 0.0014 |
Bud | 0.0007 | 0.0023 | 0.0026 | 0.0008 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0048 | 0.0014 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0014 | 0.0025 | 0.0001 |
Bud Neck | 0.0016 | 0.0006 | 0.0012 | 0.0006 | 0.002 | 0.0049 | 0.0122 | 0.0004 | 0.0005 | 0.0021 | 0.0028 | 0.0092 | 0.0084 | 0.0003 | 0.0006 | 0.0035 | 0.001 | 0.0021 |
Bud Periphery | 0.0013 | 0.0005 | 0.0044 | 0.0013 | 0.0006 | 0.0001 | 0.0014 | 0.0001 | 0.0007 | 0.0044 | 0.0025 | 0.0002 | 0.002 | 0 | 0.0002 | 0.0019 | 0.0048 | 0.0002 |
Bud Site | 0.0064 | 0.0054 | 0.0045 | 0.0021 | 0.0115 | 0.0001 | 0.0095 | 0.0117 | 0.0019 | 0.0148 | 0.0038 | 0.0003 | 0.009 | 0.0005 | 0.0005 | 0.0016 | 0.001 | 0.0003 |
Cell Periphery | 0.0004 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.1893 | 0.2153 | 0.1606 | 0.1485 | 0.0976 | 0.1883 | 0.1622 | 0.1869 | 0.119 | 0.1582 | 0.0732 | 0.1498 | 0.1774 | 0.2499 | 0.1327 | 0.1959 | 0.1846 | 0.1813 |
Cytoplasmic Foci | 0.0259 | 0.0122 | 0.005 | 0.0454 | 0.0934 | 0.0129 | 0.0261 | 0.0041 | 0.0108 | 0.0125 | 0.0339 | 0.0061 | 0.0101 | 0.0054 | 0.0052 | 0.0071 | 0.0146 | 0.0055 |
Eisosomes | 0.0004 | 0.0002 | 0.003 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0001 |
Endoplasmic Reticulum | 0.0056 | 0.0048 | 0.0035 | 0.0027 | 0.006 | 0.0024 | 0.005 | 0.0028 | 0.0042 | 0.0023 | 0.0018 | 0.0016 | 0.004 | 0.0034 | 0.0025 | 0.0021 | 0.0031 | 0.0026 |
Endosome | 0.0226 | 0.0079 | 0.0056 | 0.027 | 0.0863 | 0.0103 | 0.018 | 0.002 | 0.0113 | 0.0053 | 0.0366 | 0.0029 | 0.0162 | 0.004 | 0.0038 | 0.0065 | 0.0067 | 0.0049 |
Golgi | 0.0088 | 0.0032 | 0.006 | 0.0078 | 0.0247 | 0.0062 | 0.0054 | 0.0001 | 0.0021 | 0.0032 | 0.0222 | 0.0031 | 0.0041 | 0.0001 | 0.0012 | 0.0015 | 0.0018 | 0.0006 |
Lipid Particles | 0.0209 | 0.0114 | 0.0072 | 0.0075 | 0.0314 | 0.0048 | 0.0214 | 0.0004 | 0.0029 | 0.0292 | 0.0292 | 0.0038 | 0.005 | 0.0006 | 0.0015 | 0.0047 | 0.0102 | 0.0012 |
Mitochondria | 0.0058 | 0.0057 | 0.0046 | 0.0357 | 0.0063 | 0.0014 | 0.0129 | 0.0009 | 0.0111 | 0.0078 | 0.0541 | 0.0016 | 0.0071 | 0.0005 | 0.0024 | 0.0025 | 0.0071 | 0.0065 |
None | 0.369 | 0.4071 | 0.4437 | 0.2746 | 0.2069 | 0.3691 | 0.3721 | 0.522 | 0.4559 | 0.4834 | 0.4545 | 0.2726 | 0.3613 | 0.4478 | 0.4129 | 0.3929 | 0.3753 | 0.2602 |
Nuclear Periphery | 0.0177 | 0.0176 | 0.0141 | 0.0174 | 0.0153 | 0.0118 | 0.0103 | 0.0119 | 0.0122 | 0.0071 | 0.0036 | 0.0073 | 0.0165 | 0.0139 | 0.0139 | 0.0117 | 0.0138 | 0.0109 |
Nucleolus | 0.0042 | 0.0028 | 0.0038 | 0.0035 | 0.0025 | 0.0045 | 0.0048 | 0.0028 | 0.0031 | 0.0028 | 0.0037 | 0.0046 | 0.0041 | 0.002 | 0.0021 | 0.0036 | 0.002 | 0.0037 |
Nucleus | 0.2246 | 0.2666 | 0.2838 | 0.2739 | 0.2718 | 0.3028 | 0.2633 | 0.2274 | 0.3184 | 0.1552 | 0.2311 | 0.4878 | 0.3361 | 0.2402 | 0.3983 | 0.3067 | 0.3194 | 0.482 |
Peroxisomes | 0.0079 | 0.005 | 0.0011 | 0.0242 | 0.0292 | 0.0007 | 0.0107 | 0.0001 | 0.0124 | 0.052 | 0.0197 | 0.0117 | 0.0019 | 0.0002 | 0.0016 | 0.0039 | 0.0108 | 0.0011 |
Punctate Nuclear | 0.0492 | 0.0276 | 0.0296 | 0.1184 | 0.0423 | 0.0725 | 0.0166 | 0.0208 | 0.0246 | 0.0286 | 0.008 | 0.0241 | 0.0244 | 0.0287 | 0.0156 | 0.0379 | 0.0169 | 0.0335 |
Vacuole | 0.0118 | 0.0018 | 0.0015 | 0.0024 | 0.04 | 0.001 | 0.0067 | 0.0043 | 0.0039 | 0.0008 | 0.0037 | 0.0012 | 0.0053 | 0.0018 | 0.0013 | 0.0032 | 0.0018 | 0.0017 |
Vacuole Periphery | 0.0042 | 0.0005 | 0.0004 | 0.0014 | 0.0187 | 0.0003 | 0.0018 | 0.0004 | 0.001 | 0.0003 | 0.0019 | 0.0002 | 0.0017 | 0.0003 | 0.0006 | 0.0007 | 0.0011 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.7028 | 11.9561 | 12.1989 | 8.4362 | 6.5028 | 13.3672 | 11.2073 | 10.3689 | 9.8489 | 12.6213 |
Translational Efficiency | 0.3151 | 0.2808 | 0.2429 | 0.3281 | 0.3815 | 0.2923 | 0.3988 | 0.3155 | 0.3474 | 0.3413 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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