Standard name | |
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Human Ortholog | |
Description | Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 4 | 4 | 2 | 3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5 | 1 | 10 | 5 |
Bud Neck | 0 | 0 | 2 | 2 | 6 | 4 | 7 | 8 | 4 | 1 | 0 | 0 | 2 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 2 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | – | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 127 | 54 | 307 | 283 | 285 | 395 | 434 | 482 | 236 | 211 | 160 | 185 | 180 | 297 | 375 | – | 173 | 187 | 127 | 48 | 272 | 170 | 399 | 286 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | – | 10 | 4 | 0 | 0 | 1 | 1 | 0 | 0 |
Endosome | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | – | 2 | 2 | 0 | 1 | 5 | 1 | 1 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Mitochondria | 0 | 4 | 0 | 1 | 0 | 17 | 30 | 34 | 78 | 50 | 72 | 59 | 2 | 0 | 0 | – | 5 | 2 | 2 | 0 | 8 | 4 | 4 | 7 |
Nucleus | 0 | 1 | 2 | 3 | 0 | 5 | 3 | 10 | 6 | 8 | 12 | 8 | 0 | 0 | 2 | – | 0 | 3 | 1 | 1 | 0 | 1 | 3 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 8 | 3 | 10 | 3 |
Vac/Vac Membrane | 3 | 1 | 0 | 2 | 4 | 11 | 12 | 47 | 15 | 15 | 18 | 17 | 6 | 2 | 1 | – | 0 | 8 | 1 | 12 | 1 | 3 | 2 | 5 |
Unique Cell Count | 130 | 58 | 311 | 286 | 288 | 413 | 461 | 554 | 296 | 275 | 226 | 247 | 185 | 300 | 376 | 179 | 194 | 139 | 68 | 319 | 194 | 447 | 324 | |
Labelled Cell Count | 132 | 60 | 316 | 296 | 296 | 439 | 494 | 590 | 345 | 293 | 267 | 281 | 192 | 302 | 378 | 192 | 210 | 139 | 68 | 319 | 194 | 447 | 324 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 3.9 | 5.4 | 5.3 | 5.9 | 4.6 | 4.6 | 5.1 | 4.3 | 4.8 | 5.1 | 5.6 | 5.0 | 5.1 | 4.6 | 4.6 | 5.8 | 6.3 | 6.1 | 6.0 | 6.2 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.2 | 1.5 | 1.8 | 1.5 | 1.4 | 1.5 | 1.5 | 1.5 | 1.6 | 1.7 | 1.1 | 1.2 | 1.0 | 1.6 | 2.2 | 2.2 | 2.0 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.13 | -0.22 | -0.22 | -0.07 | -0.33 | -0.15 | -0.08 | 0.05 | -0.11 | -0.08 | -0.21 | -0.21 | 0.1 | 0.22 | 0.18 | 0.16 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6316 | 0.143 | 0.3614 | 0.8476 | 0.3391 | 0.4403 | 3.0647 | 2.9144 | 3.0175 | 3.9047 | 3.3266 | 3.3081 | 0.5903 | 1.1834 | 0.7154 | 0.6029 | 0.5905 | 0.5169 |
Actin | 0.0306 | 0.0003 | 0.0036 | 0.0523 | 0.0013 | 0.0074 | 0.0232 | 0.0003 | 0.0175 | 0.0016 | 0.0056 | 0.0015 | 0.0191 | 0.0008 | 0.0044 | 0.0003 | 0.0028 | 0.0005 |
Bud | 0.0034 | 0.0016 | 0.0027 | 0.0005 | 0.0019 | 0.0011 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0019 | 0.0015 | 0.0002 | 0.0002 | 0.0001 | 0.0006 |
Bud Neck | 0.0046 | 0.0008 | 0.0019 | 0.0034 | 0.0083 | 0.0037 | 0.0088 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0011 | 0.0133 | 0.0032 | 0.0005 | 0.0002 | 0.0002 | 0.0043 |
Bud Periphery | 0.003 | 0.0013 | 0.0053 | 0.0007 | 0.0041 | 0.0019 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0041 | 0.0027 | 0.0002 | 0.0002 | 0.0002 | 0.0006 |
Bud Site | 0.0017 | 0.0049 | 0.002 | 0.0005 | 0.0161 | 0.0003 | 0.0097 | 0.0011 | 0.0265 | 0.0001 | 0.0011 | 0.0002 | 0.0057 | 0.0085 | 0.0006 | 0 | 0.0002 | 0.0017 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0008 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.4723 | 0.4138 | 0.4668 | 0.577 | 0.5878 | 0.5639 | 0.2482 | 0.3195 | 0.4252 | 0.5574 | 0.4177 | 0.5366 | 0.489 | 0.6165 | 0.5712 | 0.6056 | 0.5749 | 0.5846 |
Cytoplasmic Foci | 0.0166 | 0.0151 | 0.02 | 0.006 | 0.0272 | 0.0192 | 0.0397 | 0.0028 | 0.0057 | 0.0058 | 0.0429 | 0.0043 | 0.021 | 0.0045 | 0.0134 | 0.0037 | 0.0065 | 0.0072 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0008 | 0 | 0 | 0.0029 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.0031 | 0.0036 | 0.0031 | 0.004 | 0.0024 | 0.0026 | 0.0018 | 0.0033 | 0.0049 | 0.0011 | 0.0026 | 0.0041 | 0.0023 | 0.0036 | 0.0017 | 0.0006 | 0.0016 |
Endosome | 0.0099 | 0.0062 | 0.0104 | 0.0051 | 0.0055 | 0.0054 | 0.0139 | 0.0007 | 0.0096 | 0.0012 | 0.0118 | 0.0071 | 0.0168 | 0.0047 | 0.0037 | 0.0014 | 0.0024 | 0.0043 |
Golgi | 0.003 | 0.0002 | 0.0011 | 0.0045 | 0.0004 | 0.0006 | 0.0049 | 0.0001 | 0.0036 | 0.0003 | 0.013 | 0.0004 | 0.0048 | 0.0002 | 0.0007 | 0.0001 | 0.0028 | 0.0004 |
Lipid Particles | 0.0016 | 0.0009 | 0.0028 | 0.0058 | 0.0002 | 0.0005 | 0.0245 | 0.0001 | 0.0019 | 0.0001 | 0.0761 | 0.0007 | 0.0079 | 0.0001 | 0.0004 | 0.0014 | 0.0054 | 0.0008 |
Mitochondria | 0.0048 | 0.0006 | 0.0008 | 0.0017 | 0.0003 | 0.0006 | 0.0045 | 0.0001 | 0.0024 | 0.0002 | 0.02 | 0.0004 | 0.003 | 0.0002 | 0.0005 | 0.0018 | 0.0096 | 0.0002 |
None | 0.3971 | 0.5078 | 0.438 | 0.2515 | 0.3066 | 0.3444 | 0.4877 | 0.6653 | 0.4672 | 0.423 | 0.3462 | 0.4296 | 0.3685 | 0.3434 | 0.3843 | 0.3734 | 0.3358 | 0.3717 |
Nuclear Periphery | 0.0062 | 0.0035 | 0.0067 | 0.0058 | 0.003 | 0.0025 | 0.0584 | 0.0008 | 0.0237 | 0.0015 | 0.0017 | 0.0047 | 0.0066 | 0.0011 | 0.0024 | 0.0026 | 0.0007 | 0.0024 |
Nucleolus | 0.0005 | 0.0036 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0049 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0002 | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 |
Nucleus | 0.0229 | 0.023 | 0.0213 | 0.0675 | 0.0185 | 0.0344 | 0.0098 | 0.0046 | 0.0035 | 0.0022 | 0.004 | 0.0042 | 0.0121 | 0.0073 | 0.009 | 0.0052 | 0.007 | 0.0161 |
Peroxisomes | 0.0044 | 0.0007 | 0.0044 | 0.0048 | 0.0005 | 0.0008 | 0.0353 | 0.0001 | 0.0009 | 0.0001 | 0.0533 | 0.0005 | 0.0053 | 0.0001 | 0.0011 | 0.0002 | 0.0497 | 0.0001 |
Punctate Nuclear | 0.0055 | 0.0065 | 0.0057 | 0.0064 | 0.0112 | 0.008 | 0.0132 | 0.0016 | 0.0011 | 0.0007 | 0.0023 | 0.0049 | 0.0106 | 0.0009 | 0.0024 | 0.0006 | 0.0005 | 0.002 |
Vacuole | 0.0039 | 0.0053 | 0.0019 | 0.0018 | 0.0021 | 0.0021 | 0.0019 | 0.0003 | 0.0017 | 0.0003 | 0.0008 | 0.0005 | 0.003 | 0.0011 | 0.001 | 0.0007 | 0.0003 | 0.0006 |
Vacuole Periphery | 0.001 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0045 | 0 | 0.0046 | 0.0001 | 0.0008 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.7654 | 14.7621 | 11.9499 | 17.1962 | 22.3837 | 21.0056 | 14.5867 | 18.3164 | 21.9903 | 20.8312 |
Translational Efficiency | 1.987 | 1.3574 | 1.3033 | 1.325 | 1.4667 | 1.5751 | 0.906 | 0.597 | 0.6553 | 0.8069 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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