Standard name
Human Ortholog
Description Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.97 0.93 0.86 0.84 0.72 0.76 0.73 0.76 0.67 1.0 0.97 0.99 1.0 0.99 0.98 0.94 0.9 0.91 0.9 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.07 0.1 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.07 0.22 0.17 0.15 0.12 0.18 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 0 3 2 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 4 0 0 0 0 0 2 3 1 1 1 0 0 0 0 0 0 4 18 2 2 9 8
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 2 3 3 5 7 7 4 6 6 8 1 2 2 0 1 0 0 0 0 0 0 0
Cytoplasm 151 441 152 151 222 187 248 240 247 190 191 179 203 231 345 273 158 180 140 405 140 152 280 271
Endoplasmic Reticulum 1 0 1 0 4 12 20 5 7 9 6 6 1 1 0 11 8 3 0 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 1 1 7 20 17 26 36 0 0 0 4 1 0 0 2 1 0 0 3
Nucleus 0 0 0 1 1 1 4 6 4 8 0 7 0 1 0 0 0 0 0 10 2 5 4 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 0 1 0 0 1 0 1 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 3 0 0 0 1
Vac/Vac Membrane 0 5 5 6 9 16 22 74 55 39 30 47 0 5 5 1 1 4 0 4 2 2 5 2
Unique Cell Count 153 448 159 156 238 218 294 333 327 260 250 267 204 237 347 274 159 184 150 450 155 169 307 298
Labelled Cell Count 153 453 160 161 240 222 303 346 344 274 262 290 205 244 352 290 169 188 150 450 155 169 307 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.7 17.9 29.2 30.3 26.4 21.6 17.2 16.1 13.4 12.6 12.3 11.5 29.6 29.4 31.9 54.5 74.6 75.7 26.7 29.2 25.7
Std Deviation (1e-4) 13.3 7.0 11.0 10.4 7.5 6.7 5.2 5.4 4.8 4.5 4.4 4.9 11.0 10.0 10.2 22.2 29.1 31.9 11.2 11.0 11.3
Intensity Change (Log2) – – – 0.06 -0.14 -0.43 -0.76 -0.86 -1.13 -1.22 -1.25 -1.35 0.02 0.01 0.13 0.9 1.35 1.38 -0.13 0.0 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -1.0 -3.1 -3.6 -6.1 -5.4 -5.8 -5.1 -6.8 2.5 1.0 3.0 3.0 2.1 1.2
Endoplasmic Reticulum 0 0 2.6 3.0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.2 3.3 4.2 4.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 0.3 1.8 1.9 5.4 4.3 3.8 3.1 4.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.2918 52.1901 43.3439 39.3672 38.5488 46.2647 26.5502 37.8564 32.1733 34.8797 24.9303 35.5016 30.6395 41.6708 37.9495 33.5671 34.452 38.327
Actin 0.0389 0.0004 0.002 0.0319 0.014 0.0034 0.0147 0.0023 0.0041 0.0063 0.01 0.0023 0.0127 0.0016 0.0007 0.0011 0.0027 0.0014
Bud 0.0008 0.0003 0.0004 0.0009 0.0061 0.0002 0.0007 0.0008 0.0011 0.0014 0.0003 0.0001 0.001 0.0016 0.0003 0.0002 0.0137 0.0004
Bud Neck 0.0003 0.0001 0.0002 0.0003 0.0005 0.0005 0.0007 0.0002 0.0003 0.0008 0.0004 0.0005 0.0005 0.0002 0.0002 0.0003 0.0003 0.0007
Bud Periphery 0.0009 0 0.0001 0.0011 0.0148 0.0001 0.0005 0.0001 0.0009 0.0006 0.0003 0.0001 0.001 0.0004 0.0001 0 0.0041 0.0001
Bud Site 0.0013 0.0002 0.0005 0.0021 0.0036 0.0001 0.001 0.0005 0.0022 0.0042 0.0007 0.0001 0.0008 0.0028 0.0002 0.0001 0.0005 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0013 0 0.0002 0.0001 0.0003 0.0003 0.0001 0 0.0002 0.0001 0 0 0.0002 0
Cytoplasm 0.8227 0.993 0.9707 0.9022 0.7662 0.9481 0.8274 0.9852 0.9456 0.9061 0.9291 0.9575 0.8636 0.9856 0.9751 0.9682 0.9393 0.9561
Cytoplasmic Foci 0.0105 0.0004 0.001 0.0097 0.0131 0.0053 0.0167 0.0011 0.005 0.024 0.0071 0.0052 0.0216 0.0011 0.0054 0.0111 0.0072 0.0067
Eisosomes 0.0003 0 0.0001 0.0003 0.0005 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0072 0.0005 0.0021 0.0013 0.0041 0.0006 0.0159 0.0015 0.0034 0.0028 0.0066 0.0013 0.0028 0.0002 0.0003 0.0006 0.0037 0.001
Endosome 0.0093 0.0001 0.0012 0.0039 0.0308 0.0005 0.0342 0.0005 0.0111 0.0206 0.017 0.0042 0.0072 0.0001 0.0009 0.0011 0.004 0.0063
Golgi 0.0036 0 0.0004 0.0047 0.0254 0.0002 0.0056 0.0001 0.0006 0.0019 0.0026 0.0055 0.002 0 0.0002 0.0004 0.0008 0.0008
Lipid Particles 0.0078 0 0.0004 0.0094 0.0044 0.0034 0.0135 0.0001 0.0005 0.0027 0.0042 0.0017 0.0111 0 0.0041 0.0043 0.0041 0.0005
Mitochondria 0.0134 0.0001 0.0012 0.0028 0.0717 0.0003 0.0045 0.0004 0.0004 0.0012 0.001 0.0012 0.0033 0.0001 0.0005 0.0004 0.0004 0.0019
None 0.0209 0.0003 0.0088 0.0027 0.0214 0.0017 0.0056 0.0008 0.0083 0.0052 0.0033 0.0048 0.0121 0.0009 0.0009 0.0022 0.0074 0.0029
Nuclear Periphery 0.0212 0.0009 0.0039 0.0032 0.0029 0.0039 0.0158 0.0013 0.0024 0.0026 0.0054 0.0029 0.0096 0.0007 0.0013 0.0011 0.0018 0.0021
Nucleolus 0.0008 0 0 0.0001 0.0008 0.0045 0.0012 0 0.0002 0.0005 0.0001 0 0.0017 0 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0246 0.0033 0.0056 0.015 0.0083 0.0145 0.0223 0.0043 0.008 0.0067 0.0073 0.01 0.0262 0.0039 0.0071 0.006 0.0062 0.0113
Peroxisomes 0.0047 0 0.0001 0.0047 0.0011 0.0002 0.0045 0.0001 0.0002 0.0005 0.0008 0.0011 0.0079 0 0.0003 0.0007 0.0005 0.0002
Punctate Nuclear 0.0049 0.0001 0.0004 0.0011 0.0008 0.0116 0.0076 0.0003 0.001 0.0018 0.0019 0.001 0.009 0.0003 0.0005 0.0018 0.0009 0.0056
Vacuole 0.0035 0.0003 0.0004 0.0018 0.005 0.0007 0.005 0.0003 0.0036 0.0074 0.0011 0.0004 0.0041 0.0003 0.001 0.0002 0.0015 0.0013
Vacuole Periphery 0.0022 0.0001 0.0003 0.0006 0.0031 0.0003 0.0022 0.0001 0.0006 0.0027 0.0005 0.0001 0.0017 0.0001 0.0006 0.0001 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.0991 23.0154 8.9459 17.2378 8.7426 41.6611 49.0351 57.9637 50.1673 38.1306
Translational Efficiency 0.8792 0.7055 2.0882 1.2015 1.4813 1.1599 1.2717 1.0385 1.1246 0.9885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tmt1

Tmt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tmt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available