Standard name | |
---|---|
Human Ortholog | |
Description | DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 4 |
Bud | 0 | 1 | 1 | 0 | 2 | 4 | 6 | 11 | 9 | – | 12 | 2 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 5 | 1 | 1 | 4 | 6 |
Bud Neck | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 4 | 3 | 4 | 4 | 3 | 5 | 3 | 6 | – | 4 | 4 | 3 | 2 | 9 | 6 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 3 |
Cytoplasm | 93 | 332 | 256 | 214 | 277 | 279 | 356 | 380 | 196 | – | 130 | 147 | 261 | 285 | 304 | 89 | 87 | 85 | 99 | 311 | 198 | 181 | 167 | 213 |
Endoplasmic Reticulum | 0 | 3 | 5 | 0 | 1 | 0 | 0 | 1 | 2 | – | 5 | 1 | 1 | 3 | 0 | 6 | 3 | 3 | 0 | 3 | 8 | 7 | 12 | 23 |
Endosome | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 4 | 3 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 |
Mitochondria | 0 | 6 | 11 | 27 | 35 | 84 | 148 | 236 | 167 | – | 169 | 206 | 1 | 1 | 10 | 10 | 2 | 2 | 6 | 14 | 39 | 11 | 21 | 32 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 1 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 6 | 1 | – | 2 | 1 | 2 | 0 | 0 | 8 | 7 | 4 | 1 | 10 | 9 | 5 | 7 | 11 |
Unique Cell Count | 94 | 339 | 266 | 217 | 294 | 311 | 420 | 504 | 290 | 240 | 279 | 266 | 288 | 306 | 104 | 94 | 91 | 112 | 360 | 274 | 218 | 229 | 310 | |
Labelled Cell Count | 98 | 349 | 277 | 245 | 327 | 373 | 522 | 640 | 384 | 322 | 364 | 271 | 291 | 328 | 126 | 106 | 99 | 112 | 360 | 274 | 218 | 229 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.5 | 3.9 | 4.1 | 4.2 | 3.8 | 3.6 | 3.6 | 3.2 | – | 3.2 | 3.1 | 5.1 | 4.9 | 4.6 | 6.6 | 7.5 | 6.8 | 5.4 | 5.6 | 6.0 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.8 | 1.3 | 1.3 | 1.5 | 1.1 | 1.0 | 0.9 | – | 0.6 | 0.8 | 1.0 | 1.0 | 1.0 | 2.2 | 3.5 | 2.7 | 1.6 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.11 | -0.02 | -0.11 | -0.13 | -0.27 | – | -0.28 | -0.35 | 0.4 | 0.34 | 0.24 | 0.76 | 0.95 | 0.8 | 0.48 | 0.53 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9049 | 2.3547 | 2.672 | 2.3992 | 2.1081 | 2.4972 | 0.9472 | 0.2076 | 0.9181 | 0.5582 | 0.6121 | 0.1795 | 4.7988 | 4.7139 | 5.0887 | 3.8891 | 5.6751 | 4.6481 |
Actin | 0.0825 | 0.0073 | 0.0303 | 0.0363 | 0.0142 | 0.0077 | 0.0148 | 0.0036 | 0.0329 | 0.0185 | 0.0037 | 0.0096 | 0.0897 | 0.0047 | 0.0251 | 0.0003 | 0.0035 | 0.0143 |
Bud | 0.0026 | 0.0056 | 0.002 | 0.0285 | 0.0021 | 0.0069 | 0.0028 | 0.0084 | 0.0085 | 0.0144 | 0.0091 | 0.0025 | 0.0008 | 0.0004 | 0.0004 | 0.0001 | 0.0006 | 0.004 |
Bud Neck | 0.0072 | 0.0004 | 0.0142 | 0.0054 | 0.0019 | 0.0027 | 0.0011 | 0.0005 | 0.0027 | 0.0012 | 0.0015 | 0.001 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0027 |
Bud Periphery | 0.004 | 0.0061 | 0.0051 | 0.0234 | 0.0039 | 0.003 | 0.0049 | 0.0052 | 0.0051 | 0.0132 | 0.0227 | 0.0062 | 0.0019 | 0.0005 | 0.0005 | 0.0001 | 0.0008 | 0.0066 |
Bud Site | 0.0227 | 0.0183 | 0.0271 | 0.0396 | 0.0193 | 0.0011 | 0.0044 | 0.0287 | 0.0117 | 0.0043 | 0.0023 | 0.0005 | 0.0019 | 0.0101 | 0.0011 | 0.0002 | 0.0012 | 0.0037 |
Cell Periphery | 0.0019 | 0.0016 | 0.0024 | 0.0014 | 0.001 | 0.0003 | 0.0005 | 0.0007 | 0.0003 | 0.0008 | 0.0006 | 0.0002 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.0003 | 0.0004 |
Cytoplasm | 0.3269 | 0.6125 | 0.6336 | 0.5153 | 0.5831 | 0.6934 | 0.5394 | 0.6324 | 0.599 | 0.5886 | 0.6369 | 0.6737 | 0.4634 | 0.496 | 0.5823 | 0.6492 | 0.5709 | 0.6235 |
Cytoplasmic Foci | 0.0532 | 0.0137 | 0.0156 | 0.0306 | 0.0578 | 0.0298 | 0.0442 | 0.0292 | 0.0396 | 0.0557 | 0.0233 | 0.0446 | 0.0302 | 0.0098 | 0.0193 | 0.0071 | 0.0145 | 0.0215 |
Eisosomes | 0.0007 | 0.0002 | 0.0003 | 0.0012 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.037 | 0.004 | 0.0081 | 0.0026 | 0.0031 | 0.0052 | 0.0166 | 0.0082 | 0.0095 | 0.0094 | 0.0039 | 0.0142 | 0.0243 | 0.0057 | 0.0147 | 0.0046 | 0.0119 | 0.0047 |
Endosome | 0.0658 | 0.0084 | 0.0178 | 0.0221 | 0.0411 | 0.0126 | 0.0785 | 0.0366 | 0.04 | 0.0688 | 0.06 | 0.0479 | 0.0193 | 0.0086 | 0.0236 | 0.0126 | 0.0058 | 0.0142 |
Golgi | 0.0226 | 0.0012 | 0.0055 | 0.0171 | 0.0022 | 0.0057 | 0.0103 | 0.0018 | 0.005 | 0.0053 | 0.0082 | 0.0022 | 0.0096 | 0.0018 | 0.005 | 0.0005 | 0.0023 | 0.0017 |
Lipid Particles | 0.0307 | 0.0005 | 0.0029 | 0.0157 | 0.001 | 0.0082 | 0.0118 | 0.0006 | 0.0024 | 0.0019 | 0.0028 | 0.0021 | 0.0125 | 0.0007 | 0.0018 | 0.0002 | 0.0006 | 0.0005 |
Mitochondria | 0.0155 | 0.001 | 0.0048 | 0.019 | 0.0126 | 0.0107 | 0.0217 | 0.0021 | 0.0038 | 0.0106 | 0.0478 | 0.0027 | 0.009 | 0.0003 | 0.001 | 0.0005 | 0.0006 | 0.001 |
None | 0.2043 | 0.3096 | 0.2034 | 0.213 | 0.1821 | 0.1906 | 0.1533 | 0.2219 | 0.205 | 0.176 | 0.1434 | 0.1789 | 0.3047 | 0.4569 | 0.3142 | 0.3173 | 0.3806 | 0.2913 |
Nuclear Periphery | 0.0357 | 0.0007 | 0.0017 | 0.0008 | 0.0059 | 0.0061 | 0.0343 | 0.0017 | 0.0085 | 0.0049 | 0.0025 | 0.002 | 0.0103 | 0.0006 | 0.001 | 0.0009 | 0.001 | 0.0004 |
Nucleolus | 0.0024 | 0.0004 | 0.0007 | 0.0008 | 0.0023 | 0.0005 | 0.0035 | 0.0007 | 0.0007 | 0.0008 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0353 | 0.0015 | 0.0019 | 0.0014 | 0.0125 | 0.0031 | 0.0157 | 0.0036 | 0.006 | 0.0046 | 0.0032 | 0.0018 | 0.0024 | 0.0008 | 0.0006 | 0.0008 | 0.0009 | 0.0005 |
Peroxisomes | 0.018 | 0.0004 | 0.0086 | 0.019 | 0.0381 | 0.005 | 0.0131 | 0.0004 | 0.0041 | 0.0016 | 0.0013 | 0.0007 | 0.01 | 0.0002 | 0.0033 | 0.0001 | 0.0005 | 0.005 |
Punctate Nuclear | 0.0129 | 0.0008 | 0.0012 | 0.0017 | 0.0011 | 0.0032 | 0.0057 | 0.0024 | 0.0061 | 0.0048 | 0.0006 | 0.002 | 0.0024 | 0.0003 | 0.0013 | 0.0002 | 0.0015 | 0.0013 |
Vacuole | 0.0134 | 0.0054 | 0.0116 | 0.0042 | 0.0118 | 0.0026 | 0.0171 | 0.0094 | 0.0073 | 0.0113 | 0.0191 | 0.0055 | 0.0036 | 0.0011 | 0.0032 | 0.0042 | 0.0012 | 0.0018 |
Vacuole Periphery | 0.0046 | 0.0004 | 0.0014 | 0.0008 | 0.0024 | 0.0013 | 0.0059 | 0.0019 | 0.0018 | 0.0028 | 0.0064 | 0.0015 | 0.0019 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.8656 | 15.2381 | 14.7865 | 21.0425 | 22.3135 | 13.7603 | 20.3702 | 23.8974 | 23.1217 | 21.4712 |
Translational Efficiency | 0.8727 | 1.0811 | 1.0543 | 0.7386 | 0.7017 | 0.9917 | 0.6701 | 0.5719 | 0.5475 | 0.7453 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|