Standard name | |
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Human Ortholog | |
Description | 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 3 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 0 | 3 | 5 | 6 | 9 | 5 | 9 | 7 | 9 | 3 | 0 | 1 | 6 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 343 | 138 | 217 | 164 | 136 | 181 | 275 | 260 | 215 | 183 | 143 | 158 | 275 | 374 | 338 | 360 | 203 | 211 | 332 | 125 | 196 | 230 | 307 | 301 |
Endoplasmic Reticulum | 1 | 1 | 2 | 4 | 4 | 1 | 13 | 20 | 2 | 6 | 3 | 8 | 4 | 1 | 3 | 17 | 6 | 9 | 0 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 0 | 3 | 8 | 13 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 4 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 6 |
Unique Cell Count | 344 | 139 | 220 | 168 | 141 | 183 | 291 | 284 | 220 | 194 | 152 | 170 | 277 | 377 | 342 | 365 | 206 | 212 | 340 | 132 | 205 | 245 | 332 | 346 |
Labelled Cell Count | 347 | 139 | 223 | 168 | 146 | 187 | 295 | 290 | 224 | 200 | 156 | 178 | 282 | 377 | 346 | 388 | 217 | 224 | 340 | 132 | 205 | 245 | 332 | 346 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 128.8 | 89.7 | 104.0 | 103.3 | 81.2 | 97.4 | 94.4 | 89.1 | 86.6 | 90.7 | 93.4 | 89.4 | 112.8 | 109.8 | 105.6 | 83.3 | 107.2 | 113.1 | 107.8 | 110.4 | 115.4 |
Std Deviation (1e-4) | 20.0 | 22.0 | 26.8 | 32.6 | 25.2 | 28.8 | 24.4 | 23.8 | 26.1 | 30.3 | 30.8 | 29.6 | 29.7 | 24.4 | 29.6 | 21.7 | 29.3 | 33.1 | 30.7 | 31.7 | 37.1 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.36 | -0.09 | -0.14 | -0.22 | -0.26 | -0.2 | -0.16 | -0.22 | 0.12 | 0.08 | 0.02 | -0.32 | 0.04 | 0.12 | 0.05 | 0.09 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -1.4 | 0.2 | -2.4 | -3.5 | -0.7 | -2.4 | -2.4 | -2.9 | 0.7 | 0.7 | 0.2 | 0 | -0.1 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 2.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 138.9407 | 153.6366 | 135.183 | 102.3861 | 124.5096 | 145.332 | 156.9084 | 171.6184 | 159.6103 | 160.8865 | 133.623 | 163.4205 | 118.8375 | 147.3605 | 139.8982 | 129.7134 | 112.8217 | 127.3099 |
Actin | 0.0125 | 0.0001 | 0.0053 | 0.0214 | 0.024 | 0.0005 | 0.0059 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0015 | 0.0002 | 0.0001 | 0.0012 | 0.0323 | 0.0032 |
Bud | 0.0006 | 0 | 0.0005 | 0.0046 | 0.0011 | 0.0009 | 0.0003 | 0 | 0.0005 | 0 | 0.0017 | 0.0004 | 0.0002 | 0 | 0.0009 | 0.0003 | 0.0033 | 0.0019 |
Bud Neck | 0.0006 | 0.0001 | 0.0012 | 0.0011 | 0.0016 | 0.002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.002 |
Bud Periphery | 0.0004 | 0 | 0.0004 | 0.0057 | 0.0011 | 0.0005 | 0.0002 | 0 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0054 | 0.0006 |
Bud Site | 0.0007 | 0 | 0.0013 | 0.0027 | 0.0018 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0004 |
Cell Periphery | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0003 | 0 | 0.001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.9322 | 0.9945 | 0.923 | 0.7726 | 0.8987 | 0.95 | 0.9712 | 0.9986 | 0.9722 | 0.9984 | 0.8782 | 0.9891 | 0.8936 | 0.9987 | 0.9906 | 0.9639 | 0.8007 | 0.924 |
Cytoplasmic Foci | 0.0106 | 0.0001 | 0.0095 | 0.0543 | 0.0042 | 0.002 | 0.0025 | 0.0002 | 0.005 | 0.0001 | 0.0199 | 0.0004 | 0.0004 | 0.0001 | 0.0011 | 0.0007 | 0.0004 | 0.0019 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.0001 | 0.0005 | 0.0014 | 0.0012 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.004 | 0 | 0.0001 | 0.0049 | 0.0022 | 0.0019 |
Endosome | 0.0048 | 0 | 0.0134 | 0.0107 | 0.0191 | 0.001 | 0.0015 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0011 | 0 | 0.0003 | 0.0055 | 0.0072 | 0.0025 |
Golgi | 0.0016 | 0 | 0.0054 | 0.0092 | 0.0153 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0017 | 0.0001 |
Lipid Particles | 0.0032 | 0 | 0.0049 | 0.0233 | 0.0053 | 0.0001 | 0.0005 | 0 | 0.0008 | 0 | 0.0034 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 |
Mitochondria | 0.0019 | 0 | 0.0041 | 0.054 | 0.0083 | 0.0009 | 0.0014 | 0 | 0.0022 | 0 | 0.0092 | 0.0001 | 0.0007 | 0 | 0.0009 | 0.0008 | 0.0846 | 0.0008 |
None | 0.0085 | 0.0002 | 0.006 | 0.0061 | 0.0007 | 0.0034 | 0.0042 | 0.0001 | 0.0081 | 0.0002 | 0.0136 | 0.0017 | 0.0007 | 0.0001 | 0.0017 | 0.0003 | 0.0013 | 0.0022 |
Nuclear Periphery | 0.0046 | 0.0027 | 0.0017 | 0.0017 | 0.0051 | 0.0018 | 0.0026 | 0.0001 | 0.0004 | 0.0002 | 0.0041 | 0.0006 | 0.0518 | 0.0001 | 0.0002 | 0.0134 | 0.035 | 0.0207 |
Nucleolus | 0.0022 | 0 | 0.0043 | 0.0045 | 0.0006 | 0.0023 | 0.0019 | 0 | 0.0036 | 0 | 0.028 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0003 |
Nucleus | 0.0052 | 0.0017 | 0.005 | 0.006 | 0.0043 | 0.0127 | 0.0028 | 0.0004 | 0.0023 | 0.0009 | 0.0172 | 0.0042 | 0.0419 | 0.0006 | 0.0012 | 0.0058 | 0.0109 | 0.0334 |
Peroxisomes | 0.0015 | 0 | 0.0031 | 0.004 | 0.0028 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0022 | 0 | 0.0043 | 0.0029 | 0.0012 | 0.0003 | 0.0003 | 0 | 0.0003 | 0 | 0.001 | 0.0002 | 0.0005 | 0 | 0 | 0.0002 | 0.0003 | 0.0028 |
Vacuole | 0.0032 | 0.0001 | 0.0026 | 0.0061 | 0.002 | 0.0168 | 0.0015 | 0 | 0.0018 | 0 | 0.0088 | 0.0015 | 0.0013 | 0 | 0.0016 | 0.0013 | 0.0025 | 0.0007 |
Vacuole Periphery | 0.0012 | 0 | 0.0029 | 0.0068 | 0.0013 | 0.0035 | 0.0008 | 0 | 0.0014 | 0 | 0.0099 | 0.0003 | 0.001 | 0 | 0.0006 | 0.0011 | 0.0104 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 579.8363 | 563.1177 | 678.2221 | 708.7137 | 702.8505 | 621.3501 | 753.575 | 739.267 | 875.7942 | 674.6915 |
Translational Efficiency | 1.7955 | 2.0241 | 1.8433 | 2.1567 | 2.1094 | 1.9553 | 1.7193 | 2.1039 | 1.6527 | 1.9371 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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