Standard name | |
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Human Ortholog | |
Description | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 7 | 2 | 32 | 12 | 29 | 20 |
Bud | 0 | 0 | 0 | 1 | 0 | 3 | 4 | 4 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 7 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 3 | 0 | 3 | 1 | 5 | 8 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 0 | 2 | 1 | 3 | 3 | 3 | 3 | 5 | 2 | 1 | 2 | 3 | 3 | 3 | 10 | 3 | 2 | 0 | 0 | 0 | 3 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 0 | 2 | 0 | 3 | 5 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 0 | 1 | 0 | 2 | 3 | 4 | 4 | 5 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 19 | 10 | 10 | 6 | 1 | 14 | 7 | 15 | 42 | 66 | 46 |
Mitochondria | 49 | 11 | 91 | 22 | 74 | 172 | 214 | 201 | 122 | 165 | 139 | 175 | 55 | 87 | 106 | 11 | 13 | 3 | 21 | 12 | 50 | 36 | 52 | 55 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 2 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 30 | 10 | 72 | 12 | 75 | 183 | 302 | 336 | 179 | 250 | 201 | 219 | 15 | 23 | 26 | 33 | 44 | 13 | 2 | 5 | 14 | 9 | 21 | 21 |
Peroxisomes | 8 | 0 | 37 | 3 | 33 | 32 | 26 | 29 | 7 | 10 | 15 | 12 | 32 | 26 | 25 | 3 | 3 | 2 | 22 | 9 | 128 | 22 | 50 | 44 |
SpindlePole | 6 | 0 | 18 | 2 | 7 | 12 | 12 | 16 | 2 | 6 | 1 | 4 | 13 | 20 | 14 | 1 | 3 | 1 | 7 | 5 | 26 | 6 | 20 | 19 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 3 | 0 | 0 | 1 | 7 | 1 | 3 | 3 | 0 | 0 | 3 | 0 | 3 | 8 | 12 |
Unique Cell Count | 72 | 16 | 173 | 31 | 157 | 285 | 418 | 451 | 235 | 322 | 277 | 307 | 99 | 150 | 159 | 66 | 64 | 19 | 82 | 54 | 287 | 147 | 267 | 240 |
Labelled Cell Count | 97 | 21 | 225 | 41 | 200 | 419 | 575 | 614 | 321 | 447 | 364 | 416 | 124 | 191 | 190 | 73 | 77 | 23 | 82 | 54 | 287 | 147 | 267 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.5 | 18.4 | 30.5 | 29.0 | 28.7 | 29.9 | 34.2 | 37.3 | 36.7 | 40.4 | 39.2 | 38.7 | 32.2 | 31.8 | 32.5 | 45.3 | 42.2 | 39.6 | 30.9 | 34.1 | 36.8 |
Std Deviation (1e-4) | 10.8 | 6.0 | 13.2 | 13.7 | 13.3 | 16.1 | 18.4 | 21.1 | 20.6 | 23.4 | 21.6 | 20.5 | 15.0 | 13.8 | 12.0 | 21.3 | 18.2 | 15.0 | 12.3 | 14.8 | 16.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.09 | -0.03 | 0.16 | 0.29 | 0.27 | 0.4 | 0.36 | 0.34 | 0.08 | 0.06 | 0.09 | 0.57 | 0.47 | 0.37 | 0.02 | 0.16 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | -1.0 | 1.6 | -0.3 | -1.8 | -0.1 | -0.3 | -0.5 | 0.9 | 0.5 | 1.0 | 2.6 | -5.0 | -4.4 | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.3 | 1.1 | 4.7 | 7.0 | 7.7 | 7.1 | 8.0 | 6.5 | 6.4 | -4.5 | -5.2 | -5.0 | 1.2 | 3.7 | 2.2 |
Peroxisomes | -1.5 | -0.1 | -2.9 | -5.4 | -5.4 | -5.9 | -6.6 | -5.2 | -6.1 | 2.0 | -0.9 | -1.3 | -3.1 | -3.0 | 0 |
SpindlePole | 0 | -2.0 | -2.6 | -3.8 | -3.4 | -4.4 | -4.2 | -5.2 | -4.6 | 0.7 | 0.8 | -0.5 | -2.3 | -1.4 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 51.0546 | 80.6871 | 68.4133 | 68.4764 | 74.1109 | 72.0747 | 42.0362 | 87.9063 | 68.7697 | 71.3378 | 78.5379 | 73.3739 | 47.1731 | 75.1659 | 70.3585 | 65.1208 | 79.5454 | 66.8448 |
Actin | 0.0044 | 0.0014 | 0.0017 | 0.0002 | 0.0002 | 0.0006 | 0.0086 | 0.0056 | 0.0031 | 0.0003 | 0.0003 | 0.001 | 0.0191 | 0.0073 | 0.0026 | 0.0004 | 0.0003 | 0.0003 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.0005 | 0.0022 | 0.0002 | 0 | 0.0001 | 0.002 | 0.0035 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Bud Periphery | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 | 0.0003 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0015 | 0.0016 | 0.0008 | 0.0007 | 0.0002 | 0.0001 | 0.0069 | 0.0108 | 0.003 | 0.0001 | 0.0001 | 0.0006 | 0.001 | 0.0007 | 0.0043 | 0.0005 | 0.0001 | 0 |
Cell Periphery | 0.0006 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0017 | 0.0015 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0019 | 0.0001 | 0.0004 |
Cytoplasm | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0354 | 0.0258 | 0.0186 | 0.0159 | 0.0182 | 0.0358 | 0.096 | 0.0419 | 0.0328 | 0.0492 | 0.005 | 0.0198 | 0.0268 | 0.0302 | 0.0285 | 0.067 | 0.0052 | 0.0021 |
Eisosomes | 0.0011 | 0.0004 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0017 | 0.0011 | 0.0014 | 0.0004 | 0.0003 | 0.0007 | 0.0014 | 0.0008 | 0.0006 | 0.0006 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0017 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0021 | 0.0006 | 0.0011 | 0.0001 | 0 | 0.0009 | 0.0047 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0001 |
Golgi | 0.0017 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0052 | 0.0043 | 0.0019 | 0.0003 | 0.0002 | 0.0023 | 0.0033 | 0.0026 | 0.0032 | 0.0005 | 0.0002 | 0.0005 |
Lipid Particles | 0.0589 | 0.0463 | 0.0211 | 0.0227 | 0.0129 | 0.0443 | 0.1176 | 0.0544 | 0.0473 | 0.0415 | 0.0247 | 0.0814 | 0.101 | 0.0647 | 0.0699 | 0.0776 | 0.0059 | 0.063 |
Mitochondria | 0.7875 | 0.7744 | 0.8437 | 0.8304 | 0.8941 | 0.8425 | 0.5924 | 0.7037 | 0.7519 | 0.7482 | 0.8999 | 0.7964 | 0.7449 | 0.721 | 0.7826 | 0.7298 | 0.9252 | 0.8701 |
None | 0.001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0042 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0004 | 0.0053 | 0 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0206 | 0.0275 | 0.0269 | 0.0063 | 0.0244 | 0.0039 | 0.0534 | 0.0385 | 0.0345 | 0.0344 | 0.016 | 0.0262 | 0.013 | 0.0681 | 0.0196 | 0.029 | 0.0027 | 0.0167 |
Nucleus | 0.0008 | 0.0011 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.002 | 0.0017 | 0.001 | 0.0009 | 0.0004 | 0.0007 | 0.0006 | 0.0019 | 0.0006 | 0.0015 | 0.0001 | 0.0007 |
Peroxisomes | 0.0719 | 0.0997 | 0.0732 | 0.1224 | 0.0489 | 0.0665 | 0.0848 | 0.1099 | 0.1003 | 0.0955 | 0.0512 | 0.0603 | 0.0726 | 0.0909 | 0.0795 | 0.0662 | 0.0592 | 0.0317 |
Punctate Nuclear | 0.0012 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0026 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0 |
Vacuole | 0.0021 | 0.0015 | 0.0019 | 0.0001 | 0.0001 | 0.0003 | 0.0034 | 0.004 | 0.0018 | 0.0032 | 0.0002 | 0.0008 | 0.0014 | 0.0016 | 0.0007 | 0.0054 | 0.0001 | 0.0012 |
Vacuole Periphery | 0.0072 | 0.0131 | 0.0071 | 0.0003 | 0.0003 | 0.0048 | 0.0153 | 0.0169 | 0.0132 | 0.0249 | 0.001 | 0.0079 | 0.0067 | 0.0089 | 0.0061 | 0.0183 | 0.0006 | 0.0126 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 566.1172 | 558.3998 | 547.6463 | 571.6328 | 517.6482 | 490.7126 | 613.5556 | 577.4604 | 635.8259 | 576.2207 |
Translational Efficiency | 1.2499 | 1.258 | 1.2506 | 1.3634 | 1.2536 | 1.3762 | 1.1383 | 1.2987 | 1.1038 | 1.0492 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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