Standard name
Human Ortholog
Description Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.09 0.11 0.1 0.14 0.1 0.14 0.08 0.09 0.09 0.1 0.08 0.09 0.21 0.23 0.12 0.17 0.13 0 0 0.05 0.09 0.08 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0.09 0 0 0 0 0 0 0 0.1 0.09 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.21 0.41 0.16 0.34 0.37 0.39 0.47 0.52 0.46 0.48 0 0 0.08 0 0 0 0 0 0 0 0 0
Nucleus 0.5 0.47 0.55 0.49 0.34 0.46 0.41 0.42 0.49 0.46 0.49 0.45 0.37 0.29 0.23 0.5 0.44 0.41 0.53 0.49 0.5 0.71 0.58 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.27 0.14 0.26 0.39 0.27 0.27 0.32 0.26 0.28 0.27 0.27 0.31 0.35 0.39 0.13 0.18 0.22 0.17 0.14 0.09 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.15 0 0 0
Vac/Vac Membrane 0.27 0.24 0.16 0.09 0.11 0.11 0.08 0.06 0 0 0.05 0.06 0.23 0.24 0.22 0.29 0.22 0.25 0 0.07 0.09 0.06 0.15 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 2 0 0 0
Bud 0 0 0 0 3 4 0 7 6 3 2 7 0 0 0 0 0 0 16 17 7 3 12 11
Bud Neck 0 0 1 0 1 1 0 1 2 0 1 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud Site 0 0 1 1 2 6 12 9 15 17 8 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 30 17 22 19 41 30 65 39 28 41 26 27 12 29 33 24 34 18 14 5 12 7 18 36
Endoplasmic Reticulum 0 1 1 0 0 0 2 0 0 1 0 0 0 0 0 8 7 5 0 0 1 0 1 1
Endosome 19 7 7 1 25 2 4 2 2 1 0 1 13 12 4 8 6 5 11 5 3 2 5 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0
Mitochondria 1 12 44 75 46 101 170 197 153 228 116 167 4 1 12 2 3 2 2 5 5 0 1 2
Nucleus 168 87 113 88 97 137 186 210 158 201 123 154 47 41 34 98 86 57 197 109 122 53 122 109
Nuclear Periphery 0 0 0 0 1 2 5 1 2 2 1 0 0 0 1 0 0 0 0 0 1 0 0 1
Nucleolus 81 50 30 47 111 80 123 163 85 121 67 94 40 50 57 25 35 30 62 30 22 1 8 8
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 4 7 3 9 5 8 8 2 5 2 2 4 4 0 1 1 0 43 27 35 0 5 8
Vac/Vac Membrane 92 45 33 17 32 32 35 28 11 16 13 19 30 34 32 57 43 34 15 16 22 4 32 49
Unique Cell Count 339 184 207 181 286 297 459 503 325 438 251 345 128 141 145 195 196 138 372 223 243 76 213 247
Labelled Cell Count 394 223 259 251 368 400 610 666 464 637 359 488 151 172 174 224 217 153 372 223 243 76 213 247


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.7 6.5 5.6 5.5 6.3 5.6 5.3 5.3 5.1 5.0 5.1 4.8 8.6 8.4 7.6 13.0 13.8 13.7 8.4 8.3 8.7
Std Deviation (1e-4) 1.8 1.3 1.0 1.3 1.7 1.4 1.4 1.2 1.4 1.4 1.3 1.6 1.9 2.0 2.0 3.1 3.3 3.1 0.8 1.6 1.4
Intensity Change (Log2) – – – -0.03 0.18 0.0 -0.07 -0.08 -0.14 -0.17 -0.14 -0.21 0.62 0.59 0.45 1.21 1.3 1.29 0.58 0.57 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.7 2.4 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.2 -0.2 1.3 -1.2 -0.8 -0.5 -0.1 -1.1 -0.4 2.6 3.1 0.5 1.9 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.4 0 0 0 0 0 0 0 2.5 2.1 0 0.4 -0.2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 -1.5 3.1 4.0 4.6 6.0 7.4 5.6 0 -4.6 -5.6 -3.3 -6.4 -6.2 -5.3
Nucleus -1.2 -4.6 -1.9 -3.4 -3.1 -1.3 -2.1 -1.2 -2.3 -3.2 -4.7 -5.8 -0.9 -2.1 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.8 5.9 3.3 3.5 4.9 3.2 3.7 3.2 0 3.7 4.6 5.3 -0.5 0.9 1.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 -0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -1.5 -1.7 -3.3 -4.5 -5.1 -5.5 -3.8 -4.1 1.7 1.9 1.5 3.2 1.5 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0794 7.4814 7.0834 6.5921 5.4614 6.2397 5.9481 7.4915 7.5046 6.8542 6.0522 6.7109 6.771 8.4089 8.1617 7.5565 7.105 7.4575
Actin 0.0003 0.001 0.0002 0.0003 0 0.0002 0.0218 0.0045 0.0092 0.0005 0.0853 0.0017 0.0036 0.0002 0.0022 0.0013 0.0001 0.0001
Bud 0.0003 0.0005 0.0002 0.0005 0 0.0001 0.0013 0.0022 0.0002 0.0004 0.0004 0.0002 0.0002 0 0.0001 0.0003 0.0001 0.0001
Bud Neck 0.0008 0.0007 0.0014 0.0016 0 0.0025 0.002 0.0008 0.0006 0.0013 0.0007 0.0027 0.0015 0.0004 0.0006 0.0011 0.0022 0.0022
Bud Periphery 0.0002 0.0005 0.0002 0.0004 0 0.0001 0.0013 0.0008 0.0002 0.0008 0.0004 0.0002 0.0005 0 0.0001 0.0001 0.0002 0.0001
Bud Site 0.0007 0.0065 0.0013 0.0023 0 0.0002 0.0096 0.0082 0.0008 0.0007 0.0008 0.0002 0.0005 0.0004 0.0006 0.0006 0.0011 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0003 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0 0.0003 0
Cytoplasm 0.0092 0.0068 0.0018 0.0012 0.0002 0.0023 0.0314 0.0406 0.0039 0.0005 0.0036 0.0007 0.0023 0.0116 0.001 0.0019 0.0029 0.0008
Cytoplasmic Foci 0.0011 0.0015 0.0013 0.0025 0 0.0001 0.0351 0.0022 0.0008 0.0036 0.0275 0.0013 0.0006 0.0001 0.0005 0.0017 0.0015 0.0001
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0009 0.0004 0.0001 0.0001 0 0.0003 0.011 0.0003 0.0008 0.0006 0.0059 0.0002 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001
Endosome 0.0018 0.0004 0.0006 0.0011 0 0.0005 0.0396 0.0004 0.0022 0.0305 0.112 0.0044 0.0009 0.0002 0.0008 0.0004 0.003 0.0004
Golgi 0.0001 0.0001 0.0001 0.0004 0 0 0.0174 0.0001 0.0013 0.024 0.1036 0.005 0.0005 0 0.0003 0.0001 0.0007 0
Lipid Particles 0.0002 0.0001 0.0002 0.0005 0 0 0.0225 0.0001 0.0007 0.0014 0.0096 0.0007 0.0015 0 0.0015 0.0001 0.0071 0.0001
Mitochondria 0.001 0.0006 0.0003 0.0007 0.0001 0.0002 0.0114 0.0004 0.001 0.0019 0.0143 0.0007 0.0005 0.0001 0.0003 0.0001 0.0014 0.0001
None 0.0006 0.0003 0.0004 0.0007 0 0.0002 0.0103 0.0009 0.0005 0.0001 0.0006 0.0002 0.0012 0.0002 0.0005 0.0003 0.0001 0.0002
Nuclear Periphery 0.0269 0.0142 0.0044 0.0034 0.0006 0.0066 0.0292 0.004 0.0076 0.0012 0.0056 0.0025 0.0063 0.0056 0.0047 0.0039 0.0031 0.0031
Nucleolus 0.0479 0.0452 0.1314 0.113 0.0095 0.0265 0.0249 0.0436 0.0636 0.0894 0.0448 0.0469 0.0433 0.0434 0.0601 0.0374 0.015 0.0725
Nucleus 0.8489 0.8876 0.6776 0.6694 0.9894 0.9396 0.6657 0.8226 0.7819 0.7573 0.5755 0.9131 0.8794 0.9003 0.7777 0.7707 0.9293 0.8982
Peroxisomes 0.0001 0 0 0.0002 0 0 0.0143 0.0003 0.0004 0.0002 0.0044 0.0001 0.0007 0 0.0007 0.0003 0.001 0
Punctate Nuclear 0.0567 0.032 0.1777 0.2006 0.0001 0.0198 0.0439 0.0676 0.1232 0.0845 0.0025 0.0186 0.0553 0.0372 0.1477 0.1793 0.0274 0.0217
Vacuole 0.0015 0.0008 0.0007 0.0006 0.0001 0.0005 0.0049 0.0003 0.0007 0.0005 0.001 0.0004 0.0004 0.0001 0.0001 0.0002 0.0027 0.0002
Vacuole Periphery 0.0007 0.0004 0.0001 0.0003 0 0.0001 0.002 0.0001 0.0003 0.0005 0.0011 0.0002 0.0001 0 0.0001 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.6502 20.6501 15.9203 27.195 39.4799 35.9802 16.8797 16.2883 22.7366 43.7815
Translational Efficiency 1.0886 0.8759 0.801 0.8931 0.9978 1.3615 0.8786 0.5928 0.7003 0.8486

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
Localization
Cell Percentages nucleus (64%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Smc1

Smc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available