Standard name
Human Ortholog
Description Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0.06 0 0 0.06 0 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.94 0.93 0.95 0.87 0.61 0.66 0.6 0.64 0.6 1.0 0.98 0.95 0.94 0.9 0.92 0.89 0.92 0.91 0.84 0.87 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.11 0.2 0.17 0.21 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.08 0.26 0.18 0.22 0.13 0.13 0 0 0 0.06 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 4 1 6 3 7 0 0 0 0 0 0 1 1 0 0 1 1
Bud 0 1 12 4 1 4 19 32 31 22 34 0 1 0 0 0 0 15 10 9 5 5 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 1 1 3 0 0 0 0 0 0
Cell Periphery 1 4 3 4 4 6 7 15 12 17 23 0 1 2 0 0 1 0 0 0 0 0 0
Cytoplasm 254 300 252 455 469 488 434 449 412 340 412 76 199 216 65 110 91 247 284 259 72 152 138
Endoplasmic Reticulum 1 0 0 5 1 2 7 5 14 23 20 0 0 0 2 0 7 0 0 1 0 0 1
Endosome 0 0 0 2 1 1 7 4 4 3 6 0 0 2 0 1 0 1 0 1 0 5 1
Golgi 1 1 0 0 0 0 0 1 5 1 1 0 1 0 0 0 0 0 1 0 0 4 0
Mitochondria 3 1 3 16 11 70 79 134 114 111 141 0 0 0 0 5 2 1 0 0 0 0 0
Nucleus 2 0 0 2 0 3 21 22 20 23 28 0 0 0 0 2 1 2 2 2 1 1 4
Nuclear Periphery 0 0 0 1 0 1 16 6 19 11 10 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 2 0 0 11 5 6 2 6 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 5 4 0 5 6 0 0 0 0 0 0 0 2 3 2 0 3
Vac/Vac Membrane 4 6 12 17 24 42 186 126 148 67 91 0 3 10 4 15 8 1 2 1 1 1 1
Unique Cell Count 263 304 268 490 492 558 708 682 685 531 683 76 203 228 69 122 99 277 308 286 87 176 169
Labelled Cell Count 267 313 283 508 511 619 796 806 792 629 789 76 205 230 71 133 110 277 308 286 87 176 169


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.2 18.9 13.0 12.9 12.1 9.5 12.6 17.6 19.8 22.4 25.9 16.3 18.2 21.2 61.3 48.5 51.0 21.1 19.1 18.6
Std Deviation (1e-4) 15.5 14.8 10.5 9.5 10.4 8.8 12.1 21.0 21.3 26.5 31.1 9.5 23.1 41.8 53.2 52.6 55.4 16.2 12.4 13.4
Intensity Change (Log2) -0.01 -0.11 -0.46 -0.05 0.43 0.6 0.78 0.99 0.32 0.48 0.7 2.23 1.89 1.97 0.69 0.55 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -3.4 0 -3.7 -1.4 0.1 0 -0.2 0.3 0 -2.6 -3.2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.1 0 1.8 1.9 0 0 0 0 0 0
Cytoplasm -0.6 0.8 -2.9 -10.0 -8.9 -10.2 -9.1 -10.2 2.2 2.1 0.3 0.1 -1.4 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 3.5 2.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 0 5.4 5.0 7.3 6.6 7.5 7.6 0 0 0 0 0 0
Nucleus 0 0 0 2.9 3.0 2.8 3.5 3.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.7 0.2 1.7 7.6 5.5 6.4 3.6 3.9 0 -1.8 0 0 2.8 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 147.1001 140.8925 146.6995 185.6852 122.0993 167.1872 69.9479 90.5747 75.5569 87.2527 74.8531 100.2537 63.783 80.7772 83.0577 59.2074 66.6158 99.707
Actin 0.0042 0.0009 0.0008 0.0002 0.0007 0.0003 0.0072 0.0024 0.0033 0.0013 0.001 0.0113 0.0287 0.0045 0.0011 0.0483 0.0006 0.0006
Bud 0.0009 0.0011 0.0039 0.0014 0.0034 0.0003 0.0015 0.0073 0.0007 0.0003 0.0001 0.0006 0.0064 0.0064 0.0022 0.0024 0.0005 0.0002
Bud Neck 0.0003 0.0001 0.0003 0.0003 0.0005 0.0004 0.0005 0.0001 0.0002 0.0005 0.0004 0.0011 0.0034 0.0002 0.0001 0.0048 0.0002 0.0003
Bud Periphery 0.0003 0.0002 0.0013 0.0004 0.0008 0.0001 0.0012 0.002 0.0006 0.0001 0 0.0003 0.0031 0.001 0.0003 0.002 0.0001 0.0001
Bud Site 0.0006 0.0005 0.0004 0.0001 0.001 0 0.0013 0.0033 0.0007 0.0001 0 0.0004 0.0065 0.0028 0.0006 0.0061 0.0001 0.0003
Cell Periphery 0.0006 0.0002 0.0005 0.0001 0.0014 0.0002 0.0008 0.0005 0.0005 0.0001 0.0001 0.0002 0.001 0.0002 0.0002 0.0007 0.0001 0
Cytoplasm 0.9241 0.9807 0.9521 0.9742 0.861 0.9584 0.8479 0.9595 0.9494 0.9275 0.9752 0.9239 0.798 0.9635 0.9502 0.8196 0.9824 0.9455
Cytoplasmic Foci 0.0069 0.002 0.0018 0.0003 0.0288 0.0046 0.018 0.0081 0.0163 0.0125 0.0026 0.0041 0.03 0.0021 0.0106 0.0256 0.0055 0.0034
Eisosomes 0.0001 0 0.0001 0 0.0003 0 0.0002 0 0.0005 0 0 0 0.0005 0 0 0.0008 0 0
Endoplasmic Reticulum 0.0017 0.0003 0.0004 0.0001 0.0004 0.0004 0.006 0.0002 0.0003 0.0069 0.0005 0.0012 0.0058 0.0002 0.0006 0.0038 0.0003 0.0003
Endosome 0.0046 0 0.0001 0 0.0009 0.0001 0.0172 0.0029 0.0003 0.0289 0.0003 0.0044 0.0132 0.0009 0.0023 0.011 0.0003 0.0283
Golgi 0.0004 0 0.0001 0 0.0003 0 0.0065 0.0011 0.0002 0.0022 0.0001 0.007 0.0036 0.0011 0.0005 0.0014 0.0001 0.0095
Lipid Particles 0.0009 0.0001 0.001 0 0.0045 0.0001 0.007 0.0003 0.0006 0.0006 0.0002 0.0009 0.0132 0.0015 0.0025 0.0037 0.0001 0.0008
Mitochondria 0.0013 0.0002 0.0026 0.0001 0.0151 0.0003 0.0158 0.0006 0.0044 0.0005 0.0001 0.0017 0.0098 0.0053 0.0019 0.006 0.0001 0.0027
None 0.0064 0.0027 0.0036 0.0008 0.023 0.0026 0.0085 0.0046 0.0075 0.0038 0.0019 0.0011 0.015 0.0012 0.0021 0.036 0.0031 0.0004
Nuclear Periphery 0.0073 0.0017 0.0035 0.0026 0.0027 0.0049 0.0204 0.0012 0.0012 0.0036 0.0036 0.0041 0.0093 0.0011 0.0037 0.004 0.0011 0.0008
Nucleolus 0.0072 0.0008 0.0025 0.0001 0.0234 0.0048 0.0021 0.0005 0.0047 0.0003 0.0001 0.002 0.0058 0.0001 0.0011 0.0035 0.0001 0
Nucleus 0.0242 0.0069 0.0195 0.0183 0.0179 0.0204 0.0223 0.0036 0.0048 0.0075 0.0107 0.0315 0.0263 0.0046 0.0148 0.0084 0.0032 0.005
Peroxisomes 0.0002 0 0.0001 0 0.0013 0 0.0018 0.0002 0.0001 0.0002 0.0001 0.0004 0.0079 0.002 0.0006 0.0013 0 0.0003
Punctate Nuclear 0.0021 0.0008 0.0007 0.0002 0.0026 0.0004 0.0023 0.0005 0.0012 0.0015 0.0026 0.0024 0.0045 0.0006 0.0028 0.0029 0.0005 0.0003
Vacuole 0.0049 0.0008 0.0033 0.0007 0.006 0.0014 0.0071 0.0008 0.0017 0.0011 0.0004 0.0011 0.005 0.0006 0.0011 0.0066 0.0013 0.0007
Vacuole Periphery 0.0008 0.0001 0.0014 0.0002 0.004 0.0003 0.0046 0.0002 0.0008 0.0005 0.0001 0.0003 0.0028 0.0002 0.0006 0.0013 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0309 8.9671 6.6539 6.6087 6.5028 36.7598 22.6665 14.2784 10.6475 7.2884
Translational Efficiency 1.3813 1.3448 1.9179 2.8937 2.1972 1.4925 1.0829 2.6065 3.1232 2.7889

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
Localization
Cell Percentages cytoplasm (51%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsp12

Hsp12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available