Standard name
Human Ortholog
Description Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 – 0.06 0.09 0 0 0 0 0 0 0 0 0.05 0 0 0.12 0.12 0.18 0.13 0.19 0.07 0.15 0.19 0.19
Bud 0.09 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0.06 0.06 0.18 0.14 0.14
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.15 0.24 0.39 0.3 0.14
Bud Site 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 – 0.06 0.09 0.05 0 0 0 0 0 0 0 0.11 0.2 0.16 0.18 0.3 0.3 0.12 0.05 0.07 0 0 0
Cytoplasm 0.74 – 0.94 0.79 0.83 0.81 0.9 0.9 0.87 0.94 0.84 0.93 0.84 0.98 0.99 0.76 0.62 0.62 0.36 0.41 0.43 0.14 0.27 0.43
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 – 0 0.08 0.11 0.16 0.05 0 0.05 0 0 0 0.11 0 0 0 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 – 1 27 16 13 6 9 2 5 1 1 2 3 4 28 39 57 28 6 3 25 46 53
Bud 16 – 0 7 5 5 11 15 11 6 6 3 1 4 2 9 26 28 16 2 3 31 33 39
Bud Neck 1 – 0 9 17 11 9 18 11 2 1 1 0 3 1 0 9 1 33 5 12 65 73 41
Bud Site 13 – 0 3 9 3 1 0 0 3 1 1 1 1 0 3 5 7 – – – – – –
Cell Periphery 19 – 1 25 23 4 16 12 8 3 3 8 4 57 55 43 100 94 26 1 3 6 7 8
Cytoplasm 135 – 16 230 422 287 548 556 451 374 165 249 31 284 334 178 209 193 75 13 22 24 66 124
Endoplasmic Reticulum 3 – 0 4 0 0 0 2 0 1 0 0 0 1 3 13 14 10 8 0 0 2 4 2
Endosome 2 – 0 1 1 5 4 1 9 1 1 2 0 0 0 3 6 4 4 0 0 3 1 3
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0
Mitochondria 6 – 0 0 1 1 5 8 9 5 4 2 0 0 0 2 4 1 5 0 2 2 1 2
Nucleus 0 – 0 3 5 4 7 3 3 2 0 2 0 1 0 1 7 0 0 0 0 0 0 1
Nuclear Periphery 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 1 2 1 2 3 9 1 1 2 0 0 0 1 4 0 0 0 0 0 0 0
Peroxisomes 6 – 0 2 2 2 1 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 9 – 0 22 55 56 29 23 26 11 7 6 4 2 0 10 27 14 0 0 2 2 2 1
Vac/Vac Membrane 0 – 0 6 6 3 24 5 22 5 15 14 0 0 0 6 24 15 9 0 0 2 1 1
Unique Cell Count 182 17 290 506 355 609 617 518 400 196 269 37 290 339 234 337 312 209 33 53 170 245 286
Labelled Cell Count 239 18 341 565 395 663 655 562 419 206 291 43 356 399 297 477 427 209 33 53 170 245 286


Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 – 5.9 7.6 6.9 6.2 6.0 5.8 5.6 5.5 6.9 6.0 7.3 7.1 6.8 9.4 10.3 11.2 6.6 7.2 7.4
Std Deviation (1e-4) 1.0 – 0.6 7.2 1.8 7.4 5.5 1.2 1.2 1.0 4.2 1.2 1.2 1.5 1.2 1.8 2.0 2.0 1.3 1.2 1.5
Intensity Change (Log2) – – – 0.38 0.24 0.09 0.03 -0.02 -0.06 -0.08 0.23 0.04 0.32 0.28 0.22 0.69 0.82 0.94 0.17 0.29 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.2 -1.4 -0.6 -0.5 -0.9 -0.1 -1.1 -0.2 -1.0 1.0 1.4 -1.7 -2.7 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.805 2.4079 2.085 1.6769 1.7861 2.3802 1.6409 2.5185 2.3757 1.8601 2.0315 2.2766 1.4677 2.3262 1.819 2.3185 1.1043 1.8283
Actin 0.0156 0.0088 0.031 0.0118 0.0482 0.0198 0.0323 0.007 0.0212 0.027 0.046 0.0132 0.0186 0.0104 0.0369 0.1526 0.1203 0.0243
Bud 0.0049 0.004 0.0329 0.0139 0.0115 0.0062 0.0014 0.0017 0.0186 0.0065 0.0098 0.0027 0.0044 0.0014 0.0184 0.0433 0.0313 0.0016
Bud Neck 0.0235 0.0021 0.0107 0.0235 0.0049 0.4128 0.0203 0.006 0.0056 0.0022 0.0018 0.6708 0.02 0.0026 0.0103 0.0026 0.0759 0.6659
Bud Periphery 0.0291 0.0068 0.0837 0.1198 0.0592 0.0122 0.013 0.004 0.0793 0.0832 0.1784 0.0263 0.021 0.0017 0.0683 0.1979 0.1312 0.0084
Bud Site 0.0787 0.1913 0.0615 0.0324 0.0069 0.007 0.0455 0.1892 0.0282 0.008 0.002 0.0013 0.0601 0.108 0.039 0.0072 0.0131 0.0053
Cell Periphery 0.0407 0.0189 0.0225 0.0437 0.0485 0.0081 0.0509 0.0283 0.0298 0.0666 0.1335 0.0211 0.032 0.0196 0.0242 0.0213 0.0196 0.0083
Cytoplasm 0.5892 0.6313 0.6126 0.6289 0.5556 0.4103 0.6236 0.6394 0.7485 0.5502 0.2547 0.2416 0.6972 0.7531 0.7006 0.4924 0.4978 0.2456
Cytoplasmic Foci 0.0119 0.0088 0.0047 0.0038 0.008 0.0135 0.0114 0.0016 0.0023 0.0163 0.0136 0.0015 0.0214 0.0029 0.0107 0.0108 0.0155 0.0033
Eisosomes 0.0012 0.0004 0.0004 0.0014 0.001 0.0003 0.0006 0.0002 0.0003 0.0004 0.0008 0.0001 0.0007 0.0003 0.0005 0.0007 0.0004 0.0001
Endoplasmic Reticulum 0.008 0.0055 0.0036 0.006 0.0072 0.0027 0.0164 0.0032 0.0073 0.0206 0.0037 0.001 0.0071 0.0028 0.0065 0.0066 0.0034 0.0034
Endosome 0.009 0.0024 0.001 0.001 0.0114 0.0029 0.0082 0.0002 0.001 0.0293 0.0237 0.0052 0.0072 0.0003 0.0021 0.0055 0.0074 0.0006
Golgi 0.0026 0.001 0.001 0.0007 0.0087 0.0008 0.0052 0.0002 0.0013 0.0266 0.0184 0.004 0.0019 0.0003 0.0025 0.0102 0.0086 0.0004
Lipid Particles 0.0079 0.0008 0.0006 0.0005 0.003 0.0008 0.0118 0.0006 0.0022 0.0255 0.0284 0.0011 0.0045 0.0006 0.0042 0.0157 0.0154 0.0009
Mitochondria 0.0009 0.0005 0.0016 0.0009 0.003 0.0006 0.016 0 0.0024 0.044 0.0763 0.001 0.0021 0.0001 0.0009 0.0028 0.0022 0.0001
None 0.1573 0.1096 0.1233 0.1033 0.2131 0.083 0.112 0.1159 0.0463 0.0607 0.0132 0.0068 0.0745 0.093 0.0602 0.007 0.0277 0.0246
Nuclear Periphery 0.0023 0.0006 0.0006 0.0008 0.0016 0.0034 0.0059 0.0003 0.0005 0.0036 0.0271 0.0005 0.0055 0.0003 0.0009 0.0018 0.0051 0.0001
Nucleolus 0.0017 0.0015 0.0016 0.0003 0.0003 0.0017 0.0004 0.0001 0.0002 0.0008 0.0041 0.0001 0.0016 0.0003 0.0003 0.0001 0.0006 0.0024
Nucleus 0.0074 0.0018 0.0028 0.0014 0.0017 0.005 0.0019 0.0006 0.0005 0.0017 0.0119 0.0005 0.0037 0.0006 0.0012 0.0008 0.0013 0.0023
Peroxisomes 0.0014 0.0002 0.0003 0.0006 0.001 0.0005 0.0179 0 0.0025 0.0162 0.0976 0.0004 0.0036 0 0.0074 0.0139 0.0068 0.0002
Punctate Nuclear 0.0022 0.0018 0.0015 0.0006 0.001 0.0062 0.0011 0.0002 0.0002 0.001 0.0521 0.0005 0.0098 0.0003 0.003 0.0041 0.0136 0.0005
Vacuole 0.0043 0.0017 0.0021 0.0045 0.0035 0.0018 0.0032 0.0012 0.0016 0.0061 0.0018 0.0004 0.0026 0.0014 0.0019 0.0024 0.0022 0.0017
Vacuole Periphery 0.0004 0.0001 0.0002 0.0003 0.0007 0.0003 0.0009 0 0.0001 0.0032 0.0012 0.0001 0.0006 0 0.0001 0.0003 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.2647 13.6997 8.4715 23.0044 24.0091 21.6985 17.3147 15.9712 16.0636 22.8811
Translational Efficiency 1.8319 2.2542 2.6991 0.9192 1.1284 2.1621 1.5402 1.5866 1.6081 1.5495

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Mob2

Mob2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mob2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available