| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 1 | 1 | 2 |
| Bud Neck | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 5 | 5 | – | – | – | – | – | – | – | – | – | – | 1 | 6 | 7 | 15 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 346 | 587 | – | – | – | – | – | – | – | – | – | – | 199 | 237 | 242 | 540 | 342 | 436 | 337 | 598 | 16 | 162 | 208 | 284 |
| Endoplasmic Reticulum | 3 | 23 | – | – | – | – | – | – | – | – | – | – | 3 | 2 | 4 | 29 | 16 | 18 | 3 | 4 | 0 | 5 | 15 | 21 |
| Endosome | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Golgi | 0 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
| Nucleus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | 2 | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 1 | 7 | 3 | 2 | 0 | 10 | 0 | 1 | 2 | 4 |
| Unique Cell Count | 350 | 609 | 201 | 240 | 248 | 556 | 346 | 442 | 346 | 625 | 20 | 173 | 233 | 321 | ||||||||||
| Labelled Cell Count | 356 | 618 | 203 | 249 | 257 | 597 | 370 | 469 | 346 | 625 | 20 | 173 | 233 | 321 | ||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 56.9 | 56.6 | – | – | – | – | – | – | – | – | – | – | 51.3 | 46.3 | 39.7 | 63.5 | 68.5 | 67.7 | 52.2 | 57.0 | 55.4 |
| Std Deviation (1e-4) | 13.3 | 11.8 | – | – | – | – | – | – | – | – | – | – | 11.4 | 10.4 | 8.9 | 20.9 | 16.7 | 19.0 | 13.0 | 15.4 | 14.5 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 36.4896 | 50.3495 | 45.0214 | 43.2528 | 38.0049 | 45.7951 | 40.8934 | 57.6413 | 51.9737 | 50.7842 | 45.9184 | 53.2937 | 50.5676 | 62.156 | 57.1701 | 52.1128 | 51.7062 | 56.3198 |
| Actin | 0.0012 | 0.0004 | 0.0005 | 0.0001 | 0.0013 | 0.0016 | 0.0064 | 0.0012 | 0.002 | 0.0207 | 0.0012 | 0.0008 | 0.002 | 0.0002 | 0.0007 | 0.0002 | 0.0019 | 0.0011 |
| Bud | 0.0008 | 0.0007 | 0.0018 | 0.001 | 0.0015 | 0.0013 | 0.0015 | 0.0002 | 0.0011 | 0.0009 | 0.0011 | 0.0003 | 0.0009 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0005 |
| Bud Neck | 0.0008 | 0.0013 | 0.0014 | 0.0009 | 0.003 | 0.0055 | 0.0015 | 0.0002 | 0.0009 | 0.0019 | 0.0006 | 0.0022 | 0.0004 | 0.0004 | 0.0006 | 0.0011 | 0.0006 | 0.0016 |
| Bud Periphery | 0.0014 | 0.0005 | 0.0017 | 0.0006 | 0.0007 | 0.0022 | 0.002 | 0.0001 | 0.0012 | 0.0024 | 0.0029 | 0.0002 | 0.0014 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0005 |
| Bud Site | 0.0005 | 0.0011 | 0.0009 | 0.0002 | 0.0006 | 0.0003 | 0.0055 | 0.0047 | 0.002 | 0.0066 | 0.0006 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0012 | 0.0001 |
| Cell Periphery | 0.0127 | 0.0093 | 0.0093 | 0.0039 | 0.0027 | 0.0057 | 0.0039 | 0.0018 | 0.0014 | 0.0066 | 0.0024 | 0.0012 | 0.0027 | 0.0079 | 0.003 | 0.001 | 0.0014 | 0.0014 |
| Cytoplasm | 0.6542 | 0.8624 | 0.7698 | 0.7515 | 0.7115 | 0.746 | 0.7614 | 0.9537 | 0.8822 | 0.8638 | 0.7713 | 0.9051 | 0.8687 | 0.9344 | 0.9218 | 0.8229 | 0.8825 | 0.9017 |
| Cytoplasmic Foci | 0.0021 | 0.001 | 0.0019 | 0.0013 | 0.0054 | 0.0037 | 0.0111 | 0.0019 | 0.0077 | 0.0023 | 0.0058 | 0.0038 | 0.0054 | 0.0011 | 0.0032 | 0.0081 | 0.0045 | 0.006 |
| Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | 0.0979 | 0.0375 | 0.021 | 0.0083 | 0.0514 | 0.1194 | 0.0454 | 0.0154 | 0.0187 | 0.0233 | 0.032 | 0.0531 | 0.0408 | 0.0268 | 0.0229 | 0.0089 | 0.0094 | 0.0359 |
| Endosome | 0.0076 | 0.0029 | 0.0068 | 0.0097 | 0.0198 | 0.0235 | 0.0199 | 0.0016 | 0.0133 | 0.007 | 0.0476 | 0.011 | 0.0125 | 0.0019 | 0.0065 | 0.019 | 0.0284 | 0.0184 |
| Golgi | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0011 | 0.0031 | 0.0036 | 0.0003 | 0.0024 | 0.0017 | 0.0085 | 0.0023 | 0.0032 | 0.0003 | 0.0014 | 0.0009 | 0.006 | 0.0048 |
| Lipid Particles | 0.0026 | 0.0006 | 0.0011 | 0.0002 | 0.0011 | 0.0003 | 0.0073 | 0.0029 | 0.0053 | 0.0017 | 0.0045 | 0.0008 | 0.0039 | 0.0006 | 0.001 | 0.0008 | 0.0012 | 0.0016 |
| Mitochondria | 0.0021 | 0.0006 | 0.0011 | 0.0001 | 0.0006 | 0.0041 | 0.0064 | 0.0016 | 0.0138 | 0.0084 | 0.0236 | 0.0009 | 0.0032 | 0.0001 | 0.0027 | 0.0028 | 0.0038 | 0.0084 |
| None | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.007 | 0.0005 | 0.0036 | 0.0004 | 0.0026 | 0.0002 | 0.0013 | 0.0001 | 0.0002 | 0.0033 | 0.0003 | 0.0003 |
| Nuclear Periphery | 0.0366 | 0.007 | 0.0136 | 0.0077 | 0.0105 | 0.0137 | 0.0171 | 0.0013 | 0.0072 | 0.0033 | 0.0061 | 0.0048 | 0.0051 | 0.0012 | 0.0018 | 0.0031 | 0.0021 | 0.0021 |
| Nucleolus | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0009 | 0.0001 | 0 |
| Nucleus | 0.0089 | 0.003 | 0.0045 | 0.0039 | 0.0049 | 0.0037 | 0.0201 | 0.0009 | 0.0023 | 0.0015 | 0.0022 | 0.0016 | 0.0034 | 0.0007 | 0.0017 | 0.0027 | 0.0014 | 0.001 |
| Peroxisomes | 0.0073 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0017 | 0.0002 | 0.0002 | 0 | 0.0014 | 0 | 0.003 | 0.0003 | 0.0002 | 0.0002 |
| Punctate Nuclear | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0058 | 0.0003 | 0.002 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 |
| Vacuole | 0.1403 | 0.0642 | 0.1446 | 0.1833 | 0.1485 | 0.0542 | 0.0602 | 0.0101 | 0.0253 | 0.0405 | 0.0713 | 0.0087 | 0.0359 | 0.0221 | 0.0241 | 0.1025 | 0.0467 | 0.0113 |
| Vacuole Periphery | 0.0215 | 0.0066 | 0.0189 | 0.0268 | 0.0345 | 0.011 | 0.0087 | 0.0005 | 0.0049 | 0.0037 | 0.0149 | 0.0024 | 0.0058 | 0.0013 | 0.0038 | 0.0199 | 0.0071 | 0.0029 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 19.315 | 22.8598 | 26.1663 | 30.1475 | 18.5175 | 24.4703 | 27.478 | 36.2619 | 34.3916 | 27.7114 |
| Translational Efficiency | 0.5808 | 0.4792 | 0.6171 | 0.5059 | 0.6037 | 0.6282 | 0.6031 | 0.4925 | 0.5649 | 0.5889 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✘ |
| Protein Localization | ✘ |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication |
| Localization | |
| Cell Percentages | cytoplasm (100%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-2 |
Rpo41
Rpo41
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Rpo41-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
