Standard name | |
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Human Ortholog | |
Description | Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 5 | – | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 3 | 3 | 6 | 15 | 22 | – | 17 | 10 | 20 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 0 | 17 | 23 | 37 | 82 | 128 | 135 | – | 137 | 135 | 172 | 26 | 26 | 10 | 2 | 12 | 2 | 0 | 0 | 2 | 0 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 6 | 1 | 2 | 0 | 7 | 27 | – | 9 | 3 | 7 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 7 | 11 | 3 | 0 | 0 | 3 | – | 0 | 4 | 2 | 1 | 2 | 5 | 0 | 0 | 0 | 1 | 0 | 7 | 2 | 12 | 14 |
Golgi | 3 | 0 | 4 | 7 | 0 | 0 | 2 | 1 | – | 0 | 0 | 3 | 13 | 7 | 12 | 0 | 2 | 2 | 0 | 0 | 2 | 1 | 1 | 4 |
Mitochondria | 3 | 7 | 18 | 7 | 17 | 17 | 16 | 9 | – | 19 | 18 | 13 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 4 | 2 | 4 | 4 |
Nucleus | 35 | 19 | 23 | 10 | 19 | 50 | 45 | 32 | – | 20 | 21 | 20 | 32 | 32 | 44 | 1 | 3 | 1 | 21 | 5 | 8 | 23 | 38 | 32 |
Nuclear Periphery | 14 | 4 | 40 | 77 | 17 | 24 | 74 | 103 | – | 29 | 17 | 32 | 13 | 11 | 16 | 0 | 0 | 0 | 8 | 0 | 23 | 5 | 17 | 18 |
Nucleolus | 5 | 0 | 30 | 28 | 14 | 10 | 10 | 5 | – | 3 | 4 | 1 | 5 | 5 | 9 | 0 | 0 | 0 | 9 | 0 | 4 | 9 | 18 | 15 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 190 | 80 | 315 | 316 | 278 | 302 | 469 | 514 | – | 326 | 249 | 299 | 413 | 413 | 430 | 17 | 30 | 18 | 200 | 98 | 347 | 258 | 480 | 441 |
Unique Cell Count | 231 | 103 | 379 | 373 | 351 | 436 | 637 | 722 | 495 | 397 | 498 | 456 | 461 | 481 | 20 | 42 | 20 | 244 | 108 | 405 | 308 | 582 | 552 | |
Labelled Cell Count | 254 | 111 | 462 | 483 | 392 | 493 | 767 | 857 | 562 | 462 | 574 | 504 | 496 | 530 | 23 | 51 | 23 | 244 | 108 | 405 | 308 | 582 | 552 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.4 | 15.7 | 16.7 | 16.2 | 16.1 | 14.1 | 14.4 | 13.9 | – | 11.9 | 12.1 | 12.4 | 15.5 | 15.5 | 14.7 | 12.3 | 14.0 | 17.7 | 18.6 | 18.6 | 19.4 |
Std Deviation (1e-4) | 4.7 | 5.7 | 5.6 | 5.1 | 5.5 | 5.2 | 5.2 | 4.6 | – | 4.2 | 4.2 | 3.9 | 4.2 | 4.2 | 3.7 | 4.4 | 5.1 | 5.7 | 5.7 | 6.2 | 6.4 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.05 | -0.24 | -0.21 | -0.26 | – | -0.49 | -0.47 | -0.42 | -0.1 | -0.1 | -0.18 | -0.43 | -0.25 | 0.09 | 0.16 | 0.16 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 2.6 | 3.1 | – | 3.3 | 2.7 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 3.1 | 6.2 | 6.9 | 6.5 | – | 8.9 | 10.4 | 10.7 | 0.8 | 0.8 | -2.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.0 | – | 0.3 | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.0 | 0 | 0 | 0 | 0 | – | 0 | -1.0 | 0 | 0 | 0 | -1.0 | 0 | 0 | 0 |
Golgi | 0.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.8 | 0 | 1.6 | 0 | 0 | 0 |
Mitochondria | -2.2 | 0.1 | -0.6 | -1.9 | -3.6 | – | -0.7 | -0.1 | -1.7 | -4.7 | -4.7 | -4.2 | 0 | 0 | 0 |
Nucleus | -2.3 | -0.4 | 2.7 | 0.6 | -1.2 | – | -1.4 | -0.5 | -1.4 | 0.6 | 0.5 | 1.7 | 0 | 0 | 0 |
Nuclear Periphery | 3.8 | -2.9 | -2.7 | 0.5 | 1.7 | – | -2.6 | -3.3 | -2.2 | -4.5 | -4.9 | -4.3 | 0 | 0 | 0 |
Nucleolus | -0.2 | -2.2 | -3.7 | -5.0 | -6.5 | – | -5.6 | -4.7 | -6.1 | -4.9 | -4.9 | -4.2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | -1.4 | -4.6 | -3.5 | -4.4 | – | -5.7 | -6.4 | -7.4 | 3.2 | 2.7 | 2.7 | 0.2 | -1.9 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.9939 | 15.4974 | 14.0542 | 12.8686 | 12.2723 | 13.9908 | 11.9477 | 18.0569 | 15.2473 | 14.3973 | 13.781 | 15.4531 | 12.9334 | 18.2484 | 15.5484 | 15.8003 | 14.7995 | 15.9997 |
Actin | 0.0026 | 0 | 0.0048 | 0 | 0.0102 | 0 | 0.0161 | 0 | 0.0002 | 0.0024 | 0.0031 | 0.0053 | 0.0003 | 0 | 0.0019 | 0 | 0 | 0 |
Bud | 0.0009 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 |
Bud Neck | 0.0011 | 0.0001 | 0.0016 | 0 | 0.0006 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0026 | 0.0001 | 0.001 | 0 | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Bud Site | 0.0019 | 0.0001 | 0.0069 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0004 | 0.0006 | 0.0018 | 0 | 0.0004 | 0 | 0.0036 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0018 | 0.0012 | 0.0015 | 0.0004 | 0.0009 | 0.0003 | 0.0015 | 0.0008 | 0.0018 | 0.0004 | 0.0004 | 0.0007 | 0.0016 | 0.0007 | 0.0018 | 0.0008 | 0.0009 | 0.0008 |
Cytoplasm | 0.0056 | 0.0066 | 0.012 | 0.0001 | 0.0017 | 0.0009 | 0.0011 | 0.0013 | 0.0005 | 0.0016 | 0.0002 | 0 | 0.0003 | 0.0012 | 0.0003 | 0 | 0.0003 | 0.0008 |
Cytoplasmic Foci | 0.0051 | 0.001 | 0.0041 | 0 | 0.002 | 0.0001 | 0.0071 | 0 | 0.0024 | 0.0081 | 0.0069 | 0.0001 | 0.0061 | 0.0001 | 0.0017 | 0 | 0.0008 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0383 | 0.0109 | 0.0021 | 0 | 0.0022 | 0.0031 | 0.001 | 0.0001 | 0.0003 | 0.0027 | 0.0001 | 0.0001 | 0.0004 | 0.0017 | 0.0002 | 0.0001 | 0.0002 | 0.0015 |
Endosome | 0.0193 | 0.0036 | 0.0095 | 0.0036 | 0.0189 | 0.0036 | 0.0164 | 0.0023 | 0.0143 | 0.0211 | 0.008 | 0.0025 | 0.0082 | 0.0069 | 0.0051 | 0.0041 | 0.0062 | 0.0039 |
Golgi | 0.0035 | 0.0001 | 0.0011 | 0 | 0.0021 | 0 | 0.0038 | 0 | 0.0026 | 0.0023 | 0.006 | 0.0005 | 0.0008 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0001 |
Lipid Particles | 0.0092 | 0.0009 | 0.0024 | 0.0001 | 0.0035 | 0.0004 | 0.0103 | 0.0004 | 0.0056 | 0.0112 | 0.0058 | 0.0012 | 0.0076 | 0.0002 | 0.0047 | 0.0002 | 0.001 | 0.0018 |
Mitochondria | 0.0099 | 0.0005 | 0.0053 | 0.0001 | 0.0016 | 0.0002 | 0.0145 | 0.0004 | 0.0199 | 0.0032 | 0.0233 | 0.0003 | 0.0116 | 0.0007 | 0.0102 | 0.0006 | 0.0021 | 0.0013 |
None | 0.0011 | 0 | 0.0012 | 0 | 0.0039 | 0 | 0.0025 | 0 | 0.006 | 0.0011 | 0.0001 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0098 | 0.0061 | 0.0038 | 0.0003 | 0.0209 | 0.0144 | 0.0072 | 0.0027 | 0.0021 | 0.0086 | 0.0009 | 0.0041 | 0.0026 | 0.0097 | 0.0041 | 0.0003 | 0.0008 | 0.0045 |
Nucleolus | 0.0062 | 0.0053 | 0.0043 | 0.0002 | 0.0061 | 0.0001 | 0.0034 | 0.0002 | 0.0057 | 0.0008 | 0.0003 | 0.0006 | 0.0021 | 0.0002 | 0.0065 | 0.0002 | 0.0036 | 0.0026 |
Nucleus | 0.0135 | 0.0062 | 0.0098 | 0.0003 | 0.0099 | 0.001 | 0.0035 | 0.0005 | 0.0028 | 0.002 | 0.0004 | 0.0006 | 0.0029 | 0.0012 | 0.0031 | 0.0003 | 0.0022 | 0.0034 |
Peroxisomes | 0.0031 | 0 | 0.0019 | 0 | 0.0006 | 0 | 0.0032 | 0 | 0.0004 | 0.0007 | 0.0119 | 0.0001 | 0.0072 | 0 | 0.0114 | 0 | 0.0002 | 0 |
Punctate Nuclear | 0.0012 | 0 | 0.0054 | 0 | 0.01 | 0.0001 | 0.0046 | 0 | 0.0003 | 0.0077 | 0.0001 | 0 | 0.0033 | 0 | 0.0007 | 0 | 0 | 0 |
Vacuole | 0.3435 | 0.3932 | 0.3536 | 0.4257 | 0.3404 | 0.3039 | 0.2881 | 0.3013 | 0.2627 | 0.3013 | 0.286 | 0.2176 | 0.3381 | 0.3074 | 0.2894 | 0.3763 | 0.3433 | 0.2306 |
Vacuole Periphery | 0.5199 | 0.5639 | 0.5661 | 0.5692 | 0.5636 | 0.6717 | 0.6142 | 0.6897 | 0.6703 | 0.6235 | 0.643 | 0.7661 | 0.6057 | 0.6699 | 0.6522 | 0.6171 | 0.637 | 0.7483 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 58.139 | 53.0785 | 48.6862 | 56.6781 | 50.2532 | 41.2105 | 57.9536 | 62.1309 | 54.8846 | 66.9927 |
Translational Efficiency | 1.3089 | 1.2965 | 1.4916 | 1.2216 | 1.2921 | 1.7937 | 1.4433 | 1.2152 | 1.3607 | 1.1686 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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