Standard name
Human Ortholog
Description Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.06 0.06 0.13 0.16 0.1 0.11 0.07 0.07 0 0.05 0 0.24 0.23 0.3 0.14 0.17 0.14 0 0 0 0.09 0.09 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.56 0.24 0.15 0.44 0.28 0.65 0.7 0.65 0.66 0.76 0.79 0.8 0 0 0 0.12 0.08 0.09 0 0 0 0 0 0.05
Nucleus 0.71 0.76 0.81 0.72 0.68 0.6 0.58 0.65 0.69 0.64 0.67 0.69 0.67 0.67 0.61 0.76 0.72 0.74 0.76 0.77 0.69 0.63 0.62 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07
Vac/Vac Membrane 0.07 0.16 0 0.06 0.05 0 0 0 0 0 0 0 0.08 0 0.06 0.07 0.07 0.11 0.12 0.12 0.17 0.15 0.15 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 1 1 4 3 2 5 6 3 5 0 0 0 3 0 0 0 0 3 3 5 6
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 2 3
Bud Site 2 0 0 3 3 6 8 12 8 17 10 30 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 6 11 14 29 19 29 19 27 17 11 10 39 56 87 36 65 37 3 3 3 18 26 27
Endoplasmic Reticulum 0 0 2 1 0 0 0 0 0 0 0 0 0 1 1 11 22 6 0 0 0 1 0 0
Endosome 0 0 5 1 1 1 0 0 0 0 0 0 7 14 8 1 5 3 3 0 4 2 3 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0
Mitochondria 93 25 31 48 51 120 187 176 260 292 182 220 2 4 2 30 30 25 1 1 4 4 7 14
Nucleus 118 81 162 78 126 110 155 176 272 245 153 191 107 163 175 197 273 196 128 90 152 127 176 161
Nuclear Periphery 0 0 0 0 0 2 2 0 1 1 1 3 0 0 1 0 2 0 0 1 2 0 0 0
Nucleolus 0 1 1 0 0 1 2 1 4 9 8 9 0 0 0 0 0 0 3 1 2 1 3 5
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 3 0 2 0 0 0 0 2 0 2 3 5 2 4 6 0 5 1 5 11 11 20
Vac/Vac Membrane 11 17 6 7 9 4 9 5 12 12 5 3 12 9 17 17 26 28 19 13 38 30 42 48
Unique Cell Count 167 106 200 109 185 184 268 272 393 382 229 276 160 243 287 259 377 266 170 117 222 202 284 299
Labelled Cell Count 237 130 223 153 222 267 395 391 590 601 373 473 173 253 294 300 431 297 170 117 222 202 284 299


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.6 4.3 4.3 4.0 3.4 3.6 3.6 3.5 3.2 3.2 3.2 5.8 5.2 5.2 5.7 5.7 5.5 5.6 5.7 5.8
Std Deviation (1e-4) 0.6 0.8 1.0 2.2 1.1 1.4 1.5 1.9 1.4 1.1 1.3 1.2 1.6 1.1 1.2 1.3 1.5 1.0 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.02 -0.11 -0.33 -0.26 -0.25 -0.3 -0.42 -0.45 -0.42 0.42 0.28 0.27 0.4 0.41 0.34 0.37 0.41 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.7 1.9 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1207 1.8318 1.6506 1.1944 1.4663 1.563 0.6411 1.0314 0.8458 0.8713 0.686 0.7822 -0.4282 0.3548 -0.3053 0.298 -0.3983 -0.1787
Actin 0.0023 0 0.0113 0.0001 0.0001 0.0001 0.0077 0 0.0078 0.0024 0.0164 0.009 0.0003 0 0 0.0006 0.0001 0.0001
Bud 0.0001 0 0.0001 0.0002 0 0 0.0006 0 0.0006 0.0005 0.0004 0.0001 0.0001 0 0 0.0001 0 0
Bud Neck 0.0004 0.0003 0.0003 0.0004 0.0007 0.0012 0.0148 0.0001 0.0011 0.0029 0.0017 0.001 0.0035 0.0001 0.0001 0.0003 0.0003 0.0009
Bud Periphery 0.0002 0 0.0001 0.0001 0 0 0.0013 0 0.0014 0.0014 0.001 0.0002 0.0001 0 0 0.0003 0 0
Bud Site 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0034 0.0001 0.0016 0.0069 0.0042 0.0001 0.0005 0 0 0.0003 0 0
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0001 0.0011 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.0132 0.021 0.009 0.0041 0.0071 0.0036 0.0054 0.0104 0.0052 0.0005 0.0067 0.0045 0.0126 0.0021 0.0039 0.0038 0.0162 0.002
Cytoplasmic Foci 0.0034 0.0005 0.0009 0.0003 0.0004 0.0006 0.0101 0.0003 0.0039 0.0035 0.0044 0.001 0.0017 0.0001 0.0002 0.0012 0.0001 0.0001
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0.0002 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0005 0.0003 0.0005 0.0002 0.0007 0.0001 0.0014 0.0003 0.0008 0.0002 0.0007 0.0009 0.0007 0.0001 0.0001 0.0006 0.0017 0.0002
Endosome 0.0025 0.0004 0.0006 0.0003 0.001 0.0002 0.0091 0.0003 0.0038 0.0042 0.0084 0.003 0.0026 0.0001 0.0001 0.0017 0.0007 0.0003
Golgi 0.0008 0 0.0002 0 0 0 0.002 0 0.0013 0.0034 0.0048 0.001 0.0007 0 0 0.0001 0 0
Lipid Particles 0.0079 0 0.0002 0 0.0003 0.0002 0.003 0 0.0028 0.0097 0.0258 0.0007 0.0031 0 0 0.0002 0 0.0001
Mitochondria 0.0019 0.0003 0.0004 0.0002 0.0003 0.0001 0.0045 0.0002 0.0039 0.021 0.0117 0.0026 0.0007 0.0001 0.0001 0.0037 0.0001 0.0001
None 0.073 0.0355 0.0577 0.003 0.027 0.0329 0.0105 0.0194 0.0116 0.0003 0.0243 0.0046 0.0261 0.0154 0.006 0.0024 0.0019 0.0077
Nuclear Periphery 0.0081 0.0062 0.0127 0.0078 0.0093 0.0026 0.0109 0.0108 0.0097 0.0014 0.0268 0.0113 0.0086 0.0073 0.0022 0.0114 0.0182 0.0061
Nucleolus 0.0071 0.0106 0.0063 0.0085 0.0161 0.0131 0.0128 0.0057 0.0107 0.0111 0.0133 0.013 0.0079 0.0049 0.0055 0.0054 0.0045 0.0102
Nucleus 0.8471 0.8827 0.8844 0.9346 0.9157 0.9234 0.8573 0.9403 0.9106 0.9154 0.8117 0.93 0.9175 0.9615 0.9711 0.9379 0.9502 0.9619
Peroxisomes 0.0048 0 0.0007 0 0 0 0.0021 0 0.0027 0.006 0.0063 0.0004 0.0026 0 0 0.0001 0 0
Punctate Nuclear 0.025 0.0411 0.0137 0.0398 0.0201 0.0216 0.0342 0.0113 0.018 0.0047 0.0286 0.016 0.0101 0.0081 0.0106 0.0278 0.0052 0.0101
Vacuole 0.0007 0.0007 0.0003 0.0002 0.0007 0.0001 0.0068 0.0005 0.0017 0.0023 0.0012 0.0002 0.0006 0.0001 0.0001 0.001 0.0003 0.0001
Vacuole Periphery 0.0005 0.0001 0.0001 0.0001 0.0002 0 0.0016 0.0003 0.0007 0.0007 0.0012 0.0004 0.0001 0.0001 0 0.0009 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.9364 18.3069 24.4521 28.7017 31.3311 20.4676 24.9886 21.2382 22.4332 26.348
Translational Efficiency 0.7326 1.0075 0.8232 0.5655 0.4704 0.8758 0.7672 0.8934 0.7541 0.7029

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sad1

Sad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available