ORF | |
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Human Ortholog | |
Description | Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 2 |
Bud | 3 | 1 | 5 | 3 | 4 | 4 | 10 | 14 | 13 | 9 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 3 | 6 | 5 | 4 | 11 | 8 | 2 | 3 | 0 | 1 | 0 | 6 | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 346 | 292 | 282 | 176 | 382 | 435 | 626 | 607 | 737 | 443 | 165 | 231 | 127 | 162 | 148 | 412 | 257 | 205 | 321 | 276 | 260 | 66 | 182 | 132 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 21 | 9 | 10 | 1 | 0 | 0 | 0 | 1 | 5 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 3 | 6 | 0 | 2 | 3 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 4 | 2 | 0 | 0 | 2 | 5 | 12 | 14 | 36 | 38 | 13 | 20 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 4 | 0 | 0 | 1 | 1 |
Nucleus | 2 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 2 | 1 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 1 | 1 | 2 | 7 | 1 | 14 | 14 | 4 | 11 | 1 | 1 | 2 | 0 | 0 | 9 | 13 | 3 | 19 | 8 | 7 | 1 | 5 | 8 |
Unique Cell Count | 355 | 297 | 283 | 179 | 389 | 438 | 643 | 627 | 762 | 469 | 172 | 241 | 128 | 162 | 151 | 421 | 270 | 214 | 361 | 303 | 287 | 74 | 204 | 166 |
Labelled Cell Count | 360 | 299 | 288 | 183 | 401 | 452 | 670 | 655 | 805 | 514 | 186 | 260 | 131 | 163 | 151 | 452 | 291 | 235 | 361 | 303 | 287 | 74 | 204 | 166 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 8.1 | 7.2 | 6.9 | 7.4 | 6.5 | 5.6 | 6.1 | 6.0 | 5.4 | 5.9 | 5.6 | 8.0 | 7.2 | 7.0 | 9.1 | 10.5 | 11.5 | 8.2 | 7.4 | 8.0 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.2 | 1.0 | 1.6 | 1.4 | 1.1 | 1.1 | 0.9 | 1.1 | 1.3 | 1.2 | 2.3 | 1.4 | 1.0 | 1.8 | 1.6 | 2.2 | 2.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.03 | -0.15 | -0.36 | -0.24 | -0.27 | -0.42 | -0.3 | -0.37 | 0.15 | -0.01 | -0.04 | 0.34 | 0.54 | 0.67 | 0.17 | 0.04 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.5 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.5 | -1.7 | -0.6 | -2.3 | -2.6 | -2.7 | -3.7 | -2.9 | -3.0 | -0.6 | 0.8 | -1.7 | -2.0 | -3.3 | -3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.9 | 0 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0674 | 5.3327 | 4.1436 | 4.9121 | 3.8606 | 4.9485 | 4.2805 | 6.7566 | 5.7008 | 5.681 | 5.0743 | 6.0131 | 6.3548 | 7.3472 | 6.6318 | 6.5229 | 6.3177 | 6.8964 |
Actin | 0.0742 | 0.0004 | 0.0153 | 0.0003 | 0.0003 | 0.0099 | 0.0121 | 0.0012 | 0.0039 | 0.0054 | 0.0122 | 0.0044 | 0.0391 | 0.0006 | 0.0019 | 0.0452 | 0.0008 | 0.0013 |
Bud | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0021 | 0.0014 | 0.0051 | 0.0003 | 0.001 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0001 |
Bud Neck | 0.0087 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0008 | 0.0018 | 0.0004 | 0.0005 | 0.0028 | 0.0006 | 0.0007 | 0.0359 | 0.0002 | 0.0007 | 0.0008 | 0.0005 | 0.0007 |
Bud Periphery | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0011 | 0.0017 | 0.0024 | 0.0001 | 0.0017 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | 0.0043 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0174 | 0.0175 | 0.0007 | 0.0032 | 0.0003 | 0.0001 | 0.0263 | 0.0008 | 0.001 | 0.0011 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.7401 | 0.9778 | 0.8849 | 0.9815 | 0.8968 | 0.9553 | 0.6817 | 0.8516 | 0.8513 | 0.8585 | 0.8081 | 0.9117 | 0.7628 | 0.8646 | 0.8947 | 0.8152 | 0.9444 | 0.9614 |
Cytoplasmic Foci | 0.0164 | 0.0009 | 0.0053 | 0.0013 | 0.0026 | 0.005 | 0.0598 | 0.0051 | 0.0103 | 0.0123 | 0.0464 | 0.0073 | 0.0272 | 0.0413 | 0.013 | 0.0163 | 0.006 | 0.0038 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0026 | 0.0033 | 0.0013 | 0.0017 | 0.0008 | 0.0019 | 0.003 | 0.0042 | 0.0034 | 0.0011 | 0.0021 | 0.0067 | 0.0022 | 0.003 | 0.0019 | 0.0021 | 0.0019 |
Endosome | 0.0574 | 0.0004 | 0.0025 | 0.0007 | 0.0006 | 0.0009 | 0.0359 | 0.0034 | 0.0078 | 0.0071 | 0.0117 | 0.0118 | 0.0212 | 0.0209 | 0.0231 | 0.0076 | 0.0037 | 0.0042 |
Golgi | 0.0161 | 0 | 0.0048 | 0 | 0 | 0.0009 | 0.0127 | 0.0003 | 0.0024 | 0.0008 | 0.0048 | 0.0055 | 0.0093 | 0.0042 | 0.0113 | 0.0146 | 0.0002 | 0.0015 |
Lipid Particles | 0.0114 | 0 | 0.0079 | 0 | 0 | 0 | 0.0575 | 0.0003 | 0.0034 | 0.001 | 0.022 | 0.0003 | 0.0055 | 0.0011 | 0.0081 | 0.013 | 0 | 0.0003 |
Mitochondria | 0.006 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0087 | 0.0157 | 0.0182 | 0.0007 | 0.0004 | 0.0009 | 0.0009 | 0.003 | 0.0013 | 0.0048 | 0.0078 | 0.0002 | 0.0021 |
None | 0.0255 | 0.0103 | 0.0677 | 0.0027 | 0.093 | 0.0041 | 0.024 | 0.081 | 0.1028 | 0.0934 | 0.0309 | 0.0498 | 0.0383 | 0.0487 | 0.0174 | 0.0387 | 0.036 | 0.0141 |
Nuclear Periphery | 0.0062 | 0.0024 | 0.0011 | 0.0015 | 0.0009 | 0.0006 | 0.0019 | 0.0013 | 0.0011 | 0.001 | 0.0018 | 0.001 | 0.0054 | 0.0009 | 0.0037 | 0.0007 | 0.0011 | 0.0009 |
Nucleolus | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Nucleus | 0.0032 | 0.0034 | 0.002 | 0.0087 | 0.0029 | 0.0044 | 0.0024 | 0.0035 | 0.0018 | 0.0022 | 0.0026 | 0.0022 | 0.0032 | 0.0021 | 0.0066 | 0.0066 | 0.003 | 0.0062 |
Peroxisomes | 0.0138 | 0 | 0.0021 | 0 | 0 | 0.0041 | 0.0625 | 0.0002 | 0.0064 | 0.0006 | 0.0319 | 0.0003 | 0.0054 | 0.0095 | 0.0048 | 0.0275 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.001 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0067 | 0.0006 | 0.001 | 0.001 | 0.0235 | 0.0006 | 0.0027 | 0.0006 | 0.0016 | 0.0009 | 0.0005 | 0.0006 |
Vacuole | 0.0055 | 0.0007 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0014 | 0.0019 | 0.0008 | 0.003 | 0.0004 | 0.0005 | 0.0038 | 0.0005 | 0.0022 | 0.0005 | 0.0007 | 0.0005 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0025 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0009 | 0.0003 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 73.7662 | 66.8626 | 55.9875 | 80.02 | 60.4985 | 63.781 | 76.4429 | 71.1639 | 80.5215 | 69.8623 |
Translational Efficiency | 1.4805 | 1.267 | 1.5085 | 1.1645 | 1.3219 | 1.6027 | 1.2707 | 1.1883 | 1.0934 | 1.196 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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