Standard name
Human Ortholog
Description Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.05 0.05 0.05
Bud 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.1 0 0 0 – 0 0 0 0 0.1 0.09 0.08 0.15 0.2 0.18 0 0 0.05 0.06 0.07 0.07
Cytoplasm 0.59 0.79 0.77 0.81 0.72 0.64 0.63 – 0.57 0.53 0.53 0.47 0.87 0.9 0.91 0.71 0.69 0.74 0.13 0.42 0.18 0.19 0.14 0.16
Endoplasmic Reticulum 0.23 0.16 0.16 0 0 0 0 – 0 0 0 0 0.09 0 0.05 0.06 0.07 0.06 0.51 0.25 0.45 0.5 0.53 0.47
Endosome 0.05 0 0 0 0.05 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.1 0.08 0.31 0.1 – 0.41 0.31 0.36 0.4 0 0 0 0 0 0 0.13 0.11 0.18 0.11 0.08 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0.09 0.18 0.1 0.28 – 0.15 0.17 0.14 0.16 0 0 0 0.13 0.17 0.08 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 24 15 0 4 3 0 – 0 5 4 1 16 13 6 4 4 9 25 20 32 45 59 57
Bud 4 5 3 4 5 29 8 – 23 15 29 19 4 3 0 0 1 5 6 7 5 12 24 32
Bud Neck 0 0 3 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 8 26 11 14 18 27
Bud Site 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 25 21 40 22 8 13 – 13 20 25 27 92 100 94 38 43 44 8 26 30 54 78 81
Cytoplasm 166 446 352 326 488 340 346 – 430 421 401 385 831 970 1060 180 146 183 50 293 103 190 158 179
Endoplasmic Reticulum 64 88 74 3 8 13 4 – 10 25 23 27 84 47 62 16 14 14 193 173 262 492 603 520
Endosome 13 3 2 14 36 13 30 – 21 30 20 25 11 22 9 5 4 3 6 11 5 11 12 11
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 3 0 2 2 4 4 5 3 7 6 3
Mitochondria 27 10 7 41 57 163 54 – 308 250 273 326 3 1 0 8 5 5 49 76 103 105 86 86
Nucleus 1 0 2 0 4 2 8 – 4 12 10 9 0 0 0 9 8 4 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 1 0 3 – 5 9 2 7 0 1 0 0 0 0 1 1 1 2 5 7
Nucleolus 0 0 1 0 3 1 2 – 1 2 3 1 3 1 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 3 0 0 – 0 1 0 0 0 0 1 3 0 2 0 1 0 0 0 0
SpindlePole 1 2 3 2 5 1 3 – 2 3 3 2 0 3 1 1 0 1 2 2 3 5 10 13
Vac/Vac Membrane 22 14 17 36 118 54 154 – 113 137 106 131 15 31 39 33 36 19 14 38 15 22 33 46
Unique Cell Count 282 566 460 402 674 530 551 758 797 755 821 952 1076 1170 255 212 247 379 695 589 986 1130 1102
Labelled Cell Count 318 618 501 468 754 627 625 930 930 899 962 1059 1195 1273 300 263 293 379 695 589 986 1130 1102


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.7 6.4 6.0 6.5 4.8 5.8 – 4.9 5.3 5.2 5.3 7.2 7.6 7.6 7.9 8.2 7.6 5.8 6.1 6.3
Std Deviation (1e-4) 0.8 0.8 1.0 1.0 1.1 1.2 1.4 – 0.9 0.8 0.9 0.8 1.1 1.5 1.2 1.4 1.7 1.8 1.3 1.4 1.7
Intensity Change (Log2) – – – -0.09 0.04 -0.41 -0.13 – -0.37 -0.26 -0.29 -0.26 0.18 0.25 0.25 0.31 0.36 0.26 -0.14 -0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.7 -3.4 0 -4.3 – -5.0 -3.6 -3.7 -4.9 -1.9 -2.8 -4.4 -1.3 -1.0 0.3
Bud 0 0 0 1.2 – 2.8 1.8 3.4 2.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.1 -1.1 0 -1.9 – -2.9 -2.0 -1.1 -1.2 3.3 3.2 2.5 4.8 6.5 5.8
Cytoplasm 1.6 -1.6 -4.2 -4.7 – -7.0 -8.3 -8.2 -10.3 5.1 7.1 7.5 -1.7 -2.1 -0.7
Endoplasmic Reticulum -7.9 -9.5 0 -9.1 – -9.9 -8.2 -8.1 -8.2 -4.1 -7.8 -7.1 -3.8 -3.4 -4.0
Endosome 3.3 4.5 0 4.5 – 2.9 3.6 2.8 3.1 0 2.3 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.5 5.0 0 5.5 – 15.1 12.6 13.9 14.9 0 0 0 1.4 0 0
Nucleus 0 0 0 0 – 0 1.7 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 7.1 3.9 10.2 – 6.1 7.0 5.8 6.6 -2.5 -0.8 -0.4 4.6 5.9 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6898 1.9273 1.6195 1.5338 1.3163 1.7047 3.1123 2.9537 3.1226 3.1284 2.3759 2.9554 4.9469 5.2265 5.2307 4.8777 5.1123 5.4026
Actin 0.0195 0.0051 0.0133 0.0065 0.0172 0.0098 0.0337 0.0029 0.005 0.0644 0.0444 0.0097 0.0011 0.0035 0.002 0.0023 0.0011 0.0039
Bud 0.0486 0.0832 0.0723 0.0924 0.1122 0.0432 0.0084 0.0144 0.0155 0.0075 0.0017 0.0034 0.0006 0.0009 0.005 0.0004 0.0001 0.0001
Bud Neck 0.0019 0.0024 0.0054 0.004 0.0039 0.011 0.0034 0.001 0.0018 0.0039 0.0032 0.0049 0.0005 0.0005 0.0005 0.0005 0.0008 0.0046
Bud Periphery 0.0262 0.0191 0.0276 0.0392 0.0419 0.0175 0.0058 0.0051 0.0056 0.0102 0.0023 0.0025 0.001 0.0007 0.0017 0.0006 0.0002 0.0003
Bud Site 0.0533 0.1858 0.0615 0.0399 0.0178 0.0101 0.0247 0.0359 0.0341 0.0032 0.0203 0.0006 0.0014 0.0126 0.0022 0.001 0.0002 0.0002
Cell Periphery 0.0042 0.003 0.0028 0.0029 0.0019 0.0013 0.0027 0.002 0.0011 0.0009 0.0012 0.0007 0.0015 0.003 0.0016 0.0014 0.0009 0.0011
Cytoplasm 0.3092 0.2978 0.3686 0.3206 0.3247 0.4539 0.4432 0.598 0.5249 0.5198 0.5238 0.5928 0.6489 0.779 0.7215 0.7666 0.7389 0.7491
Cytoplasmic Foci 0.0614 0.032 0.0294 0.0427 0.0373 0.0328 0.0231 0.0148 0.019 0.0588 0.0282 0.0306 0.0071 0.0123 0.0113 0.0086 0.0073 0.0156
Eisosomes 0.0008 0.0002 0.0004 0.0004 0.0003 0.0001 0.0006 0.0002 0.0002 0.0006 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0323 0.0081 0.0143 0.0281 0.0093 0.008 0.0344 0.0219 0.0161 0.0146 0.0531 0.0268 0.0533 0.0327 0.0586 0.0218 0.033 0.0453
Endosome 0.0301 0.0151 0.0219 0.0135 0.0233 0.0063 0.0166 0.0086 0.0103 0.028 0.0153 0.0122 0.0048 0.012 0.0084 0.0051 0.0147 0.0099
Golgi 0.005 0.0016 0.0049 0.0012 0.0062 0.0017 0.0044 0.0015 0.002 0.021 0.0031 0.0015 0.0006 0.0018 0.0013 0.0014 0.0006 0.0012
Lipid Particles 0.0126 0.0017 0.0058 0.0048 0.0118 0.0052 0.007 0.001 0.0025 0.0119 0.0025 0.0018 0.0017 0.0022 0.0019 0.0004 0.0012 0.0013
Mitochondria 0.0206 0.008 0.0134 0.0115 0.0074 0.0042 0.0037 0.0018 0.004 0.0067 0.0019 0.0018 0.0004 0.0003 0.0013 0.0005 0.0004 0.0008
None 0.2742 0.1751 0.2204 0.2039 0.2421 0.2219 0.342 0.2677 0.2991 0.1861 0.2598 0.2598 0.2661 0.1292 0.1649 0.1844 0.1931 0.1609
Nuclear Periphery 0.014 0.0069 0.0126 0.0161 0.0128 0.0128 0.0111 0.0025 0.005 0.006 0.0027 0.0031 0.0032 0.0018 0.0044 0.0012 0.0029 0.0014
Nucleolus 0.0129 0.0071 0.0111 0.0128 0.0052 0.0046 0.0039 0.0013 0.0043 0.0032 0.0029 0.0014 0.0001 0.0002 0.0006 0 0.0001 0.0001
Nucleus 0.0224 0.0751 0.0488 0.0714 0.0546 0.0563 0.0152 0.0092 0.0221 0.0146 0.0046 0.0084 0.0029 0.0022 0.0039 0.0014 0.0013 0.0019
Peroxisomes 0.0078 0.0009 0.0027 0.0016 0.0077 0.0048 0.0025 0.0005 0.0023 0.0085 0.0012 0.0073 0.0001 0.0001 0.0004 0.0002 0.0001 0.0003
Punctate Nuclear 0.0309 0.0666 0.0552 0.0761 0.0561 0.0898 0.0075 0.0043 0.0204 0.0249 0.0245 0.0265 0.0013 0.0013 0.006 0.0004 0.0005 0.0005
Vacuole 0.0093 0.0045 0.0059 0.0082 0.0049 0.0038 0.005 0.005 0.004 0.0039 0.0027 0.0036 0.0031 0.0034 0.0022 0.0016 0.0022 0.0014
Vacuole Periphery 0.0026 0.0006 0.0017 0.0022 0.0015 0.0009 0.0012 0.0005 0.0005 0.0011 0.0004 0.0005 0.0002 0.0002 0.0003 0.0001 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2838 4.4431 3.297 6.3259 9.6663 1.6558 5.9899 8.591 9.2326 7.0465
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (31%), cytoplasm (16%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Far7

Far7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Far7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available