Standard name | |
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Human Ortholog | |
Description | Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 |
Bud | 1 | 1 | 3 | 1 | 2 | 7 | 9 | 6 | 12 | 13 | 15 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 8 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 1 | 4 | 4 | 1 | 7 | 3 | 5 | 15 | 3 | 3 | 3 | 4 | 10 | 9 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 98 | 128 | 257 | 101 | 228 | 298 | 306 | 284 | 315 | 268 | 245 | 370 | 276 | 384 | 455 | 119 | 202 | 168 | 102 | 119 | 234 | 299 | 374 | 361 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 5 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 2 | 0 | 3 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 2 |
Mitochondria | 25 | 6 | 4 | 8 | 26 | 142 | 189 | 170 | 229 | 225 | 275 | 280 | 1 | 0 | 1 | 1 | 5 | 0 | 6 | 2 | 17 | 1 | 4 | 11 |
Nucleus | 0 | 1 | 0 | 1 | 1 | 0 | 4 | 4 | 6 | 5 | 8 | 10 | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 2 | 4 | 1 | 13 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 4 |
Vac/Vac Membrane | 1 | 1 | 1 | 0 | 3 | 3 | 13 | 18 | 66 | 34 | 47 | 37 | 0 | 0 | 1 | 5 | 4 | 2 | 0 | 11 | 0 | 0 | 1 | 3 |
Unique Cell Count | 113 | 133 | 262 | 105 | 237 | 356 | 417 | 388 | 496 | 429 | 451 | 564 | 279 | 384 | 459 | 126 | 209 | 175 | 122 | 139 | 268 | 304 | 403 | 407 |
Labelled Cell Count | 127 | 139 | 272 | 111 | 261 | 461 | 532 | 487 | 641 | 556 | 601 | 737 | 284 | 387 | 463 | 140 | 227 | 187 | 122 | 139 | 268 | 304 | 403 | 407 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.6 | 4.0 | 3.7 | 4.5 | 4.0 | 4.1 | 4.0 | 4.1 | 4.0 | 3.9 | 4.2 | 5.6 | 5.7 | 5.8 | 8.1 | 7.9 | 8.4 | 5.6 | 5.8 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.8 | 0.7 | 2.0 | 1.9 | 1.7 | 1.5 | 1.4 | 1.2 | 1.4 | 1.3 | 1.1 | 0.9 | 1.0 | 1.4 | 1.3 | 2.0 | 1.1 | 1.7 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.15 | -0.02 | 0.02 | 0.0 | 0.04 | 0.01 | -0.03 | 0.05 | 0.48 | 0.5 | 0.53 | 1.0 | 0.98 | 1.06 | 0.47 | 0.53 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1386 | 1.8994 | 1.672 | 2.0684 | 1.3288 | 1.6628 | 0.8679 | 0.9665 | 0.7863 | 0.1832 | 0.658 | 0.8026 | -0.0874 | 0.0901 | -0.1092 | -0.2864 | 0.4193 | -0.0748 |
Actin | 0.0029 | 0.001 | 0.0015 | 0.0004 | 0.0035 | 0.0018 | 0.0461 | 0.0029 | 0.0097 | 0.0001 | 0.0068 | 0.0033 | 0.027 | 0.0015 | 0.018 | 0.0082 | 0.0734 | 0.0051 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0017 | 0.0032 | 0.0007 | 0 | 0.0009 | 0.0004 | 0.0013 | 0.0011 | 0.0039 | 0.0003 | 0.001 | 0.0004 |
Bud Neck | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0034 | 0.0002 | 0.003 | 0.0001 | 0.0005 | 0.0014 | 0.0012 | 0.0003 | 0.0014 | 0.0002 | 0.0018 | 0.0024 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0025 | 0.0041 | 0.001 | 0 | 0.0024 | 0.0004 | 0.0009 | 0.0007 | 0.0028 | 0.0006 | 0.0028 | 0.0005 |
Bud Site | 0.0017 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.011 | 0.0146 | 0.0026 | 0 | 0.0011 | 0.0002 | 0.0077 | 0.0103 | 0.0073 | 0.0007 | 0.0027 | 0.001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0006 | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasm | 0.4525 | 0.5867 | 0.479 | 0.5695 | 0.49 | 0.5607 | 0.3795 | 0.5454 | 0.4864 | 0.5349 | 0.5425 | 0.6568 | 0.413 | 0.5291 | 0.5352 | 0.4184 | 0.4247 | 0.6039 |
Cytoplasmic Foci | 0.0057 | 0.0037 | 0.0086 | 0.0038 | 0.0048 | 0.0077 | 0.054 | 0.0357 | 0.069 | 0.0046 | 0.0452 | 0.0423 | 0.0192 | 0.008 | 0.0143 | 0.0079 | 0.0165 | 0.0137 |
Eisosomes | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0013 | 0.0001 |
Endoplasmic Reticulum | 0.0061 | 0.0026 | 0.0021 | 0.0019 | 0.0016 | 0.0011 | 0.0066 | 0.0025 | 0.0024 | 0.0027 | 0.0018 | 0.0019 | 0.0034 | 0.0033 | 0.003 | 0.0019 | 0.0036 | 0.0017 |
Endosome | 0.0044 | 0.0007 | 0.0057 | 0.0015 | 0.0018 | 0.0016 | 0.0381 | 0.0078 | 0.0128 | 0.0019 | 0.0095 | 0.0072 | 0.0143 | 0.003 | 0.009 | 0.0068 | 0.0271 | 0.0038 |
Golgi | 0.0019 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.009 | 0.002 | 0.004 | 0.0001 | 0.0053 | 0.0009 | 0.0044 | 0.0004 | 0.0022 | 0.0014 | 0.0177 | 0.0012 |
Lipid Particles | 0.0049 | 0.0001 | 0.0029 | 0 | 0.0001 | 0.0002 | 0.0173 | 0.0067 | 0.006 | 0.0001 | 0.0063 | 0.0025 | 0.0131 | 0.0004 | 0.0087 | 0.0074 | 0.0364 | 0.0015 |
Mitochondria | 0.0046 | 0.0001 | 0.0036 | 0.0002 | 0.0003 | 0.0001 | 0.0137 | 0.009 | 0.0037 | 0.0002 | 0.0566 | 0.0002 | 0.0057 | 0.0003 | 0.0017 | 0.0034 | 0.009 | 0.0008 |
None | 0.5082 | 0.4013 | 0.4896 | 0.4187 | 0.4924 | 0.4228 | 0.3513 | 0.3593 | 0.3629 | 0.4507 | 0.3 | 0.2707 | 0.4512 | 0.4338 | 0.3693 | 0.5207 | 0.3004 | 0.3455 |
Nuclear Periphery | 0.0011 | 0.0005 | 0.0008 | 0.0006 | 0.0007 | 0.0003 | 0.0094 | 0.0006 | 0.0023 | 0.0011 | 0.001 | 0.0014 | 0.0067 | 0.0012 | 0.0043 | 0.004 | 0.0224 | 0.0024 |
Nucleolus | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0012 | 0.0002 | 0.0006 | 0.0014 | 0.0016 | 0.0002 |
Nucleus | 0.001 | 0.0008 | 0.0011 | 0.0015 | 0.0011 | 0.0008 | 0.006 | 0.0017 | 0.0022 | 0.0019 | 0.0018 | 0.002 | 0.0066 | 0.0032 | 0.0041 | 0.0032 | 0.003 | 0.0038 |
Peroxisomes | 0.0016 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0282 | 0.0013 | 0.0196 | 0.0001 | 0.0054 | 0.0015 | 0.0139 | 0.0002 | 0.0049 | 0.0058 | 0.0267 | 0.0026 |
Punctate Nuclear | 0.0006 | 0.0005 | 0.0009 | 0.0003 | 0.0004 | 0.0012 | 0.0146 | 0.0008 | 0.0097 | 0.0006 | 0.007 | 0.0061 | 0.0059 | 0.0015 | 0.0073 | 0.0058 | 0.024 | 0.0083 |
Vacuole | 0.0008 | 0.0004 | 0.0009 | 0.0005 | 0.0006 | 0.0003 | 0.0041 | 0.0011 | 0.0011 | 0.0006 | 0.0031 | 0.0007 | 0.0018 | 0.0009 | 0.0015 | 0.001 | 0.0016 | 0.0006 |
Vacuole Periphery | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0003 | 0.0001 | 0.0018 | 0.0002 | 0.0009 | 0.0001 | 0.0004 | 0.0006 | 0.0019 | 0.0003 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.988 | 6.62 | 12.0079 | 20.9573 | 18.136 | 18.0921 | 16.1758 | 17.8195 | 20.8305 | 18.373 |
Translational Efficiency | 0.9495 | 1.1811 | 0.9544 | 0.9791 | 0.9117 | 0.9755 | 0.6024 | 0.9331 | 1.2954 | 0.9123 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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