Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFR016C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07
Bud 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0 0 0.05 0.05 0 0 0 0.05 – 0.07 0.05 0 0 0.05 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.91 0.89 0.89 0.8 0.81 0.71 0.65 0.64 0.58 – 0.53 0.5 0.98 0.98 0.98 0.9 0.89 0.92 0.83 0.86 0.75 0.66 0.67 0.67
Endoplasmic Reticulum 0.05 0.06 0.05 0.07 0.07 0.05 0 0 0 – 0 0.08 0 0 0 0.05 0 0 0 0 0 0.06 0.05 0.08
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.14 0.09 0.31 0.4 0.49 0.54 – 0.6 0.62 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0.09 0.08 0.05 0 0 0.08 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 11 5 15 6 3 7 4 2 – 3 8 0 2 3 0 0 0 11 6 8 17 23 25
Bud 4 3 1 7 6 9 10 15 16 – 9 13 0 1 9 0 0 0 9 5 4 6 12 17
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 6 5 5 11 14 9
Bud Site 0 0 0 0 0 2 2 2 1 – 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 34 15 11 9 15 10 16 12 18 – 29 20 8 10 24 9 10 7 1 0 1 1 2 2
Cytoplasm 275 301 242 156 242 216 263 264 203 – 210 201 279 498 518 180 125 148 270 312 235 162 240 251
Endoplasmic Reticulum 16 21 14 14 21 16 11 6 9 – 15 32 6 3 4 10 2 0 12 5 12 14 19 30
Endosome 0 0 0 0 0 2 9 1 2 – 0 0 0 1 0 2 0 1 2 6 2 6 6 5
Golgi 3 0 0 0 1 0 0 0 0 – 0 0 0 0 0 3 2 2 3 1 1 0 1 2
Mitochondria 1 3 8 27 28 94 163 201 188 – 235 251 2 7 27 4 1 3 1 2 12 9 8 10
Nucleus 1 2 2 2 3 4 2 1 4 – 0 0 0 1 0 4 1 2 0 0 0 0 1 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 – 2 1 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 1 0 0 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 2 1 4 0 10 7 13 19 9 – 8 10 1 2 3 18 11 8 3 11 26 12 17 9
Unique Cell Count 303 339 271 196 297 303 407 410 348 393 403 285 507 528 200 141 160 325 362 312 246 357 374
Labelled Cell Count 351 359 287 230 334 364 497 525 452 511 538 296 526 590 230 152 171 325 362 312 246 357 374


Ambiguous, Bud, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.7 8.0 7.2 6.6 7.4 6.6 6.1 5.5 5.3 – 5.2 5.0 8.1 8.6 7.9 13.4 14.5 15.2 8.2 8.0 8.3
Std Deviation (1e-4) 1.3 1.3 1.1 1.1 1.7 1.5 1.4 1.4 1.2 – 1.2 1.1 1.3 1.4 1.3 3.4 4.1 3.7 1.1 1.5 1.6
Intensity Change (Log2) – – – -0.12 0.05 -0.12 -0.23 -0.37 -0.45 – -0.45 -0.53 0.18 0.27 0.14 0.9 1.02 1.09 0.19 0.16 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.1 0.2 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Bud 0 0 0 0 2.8 3.2 – 0 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.3 0.6 -0.5 -0.1 -0.8 0.7 – 1.8 0.5 -0.8 -1.7 0.3 0.2 1.3 0.2
Cytoplasm -2.9 -2.6 -5.4 -7.2 -7.3 -8.5 – -9.7 -10.6 4.2 5.5 5.5 0.2 -0.2 1.1
Endoplasmic Reticulum 0.9 0.9 0.1 -1.7 -2.8 -1.7 – -0.8 1.4 -1.9 -4.2 -4.0 -0.1 -1.9 -2.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 4.4 3.2 8.8 10.9 12.8 13.6 – 14.9 15.5 0 -1.5 1.4 -0.6 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.5 0.7 1.4 2.2 1.0 – 0 0.9 0 0 0 3.8 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2449 9.3119 8.8945 8.5718 8.1324 8.7074 5.4781 7.307 6.5988 6.5443 5.3286 6.3993 4.5759 6.3792 5.4586 5.4895 5.2543 5.7353
Actin 0.0141 0.0084 0.0154 0.0057 0.0028 0.003 0.0792 0.0035 0.0345 0.0246 0.0277 0.0043 0.0672 0.0019 0.0641 0.0035 0.0034 0.0197
Bud 0.0148 0.006 0.0281 0.0089 0.0019 0.0005 0.0151 0.0145 0.0239 0.0026 0.0016 0.0016 0.0013 0.0007 0.0062 0.0011 0.0016 0.0009
Bud Neck 0.0045 0.0031 0.0113 0.0125 0.0012 0.0723 0.0375 0.0017 0.0012 0.0024 0.0007 0.1013 0.0012 0.0003 0.0021 0.0007 0.0463 0.0826
Bud Periphery 0.0146 0.0068 0.0218 0.0126 0.0011 0.0006 0.0137 0.0053 0.0131 0.0018 0.0027 0.0007 0.0009 0.0002 0.007 0.0006 0.0008 0.0006
Bud Site 0.06 0.1318 0.027 0.0018 0.0009 0.0008 0.025 0.121 0.0158 0.0079 0.0024 0.0008 0.0046 0.0133 0.0318 0.0025 0.018 0.0025
Cell Periphery 0.0095 0.0054 0.0039 0.0023 0.0004 0.001 0.0034 0.001 0.0007 0.0007 0.0003 0.0002 0.0011 0.0004 0.0017 0.0003 0.0002 0.0006
Cytoplasm 0.8091 0.7819 0.7662 0.8644 0.8245 0.843 0.4291 0.8213 0.7808 0.7639 0.529 0.857 0.7171 0.911 0.7927 0.8461 0.8542 0.83
Cytoplasmic Foci 0.0133 0.0092 0.0274 0.0096 0.0311 0.0118 0.0768 0.0082 0.0197 0.0089 0.0405 0.0095 0.0188 0.0332 0.0251 0.0188 0.0322 0.0166
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0005 0 0.0003 0.0005 0.0002 0 0.0014 0 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.0269 0.0097 0.0147 0.0128 0.02 0.023 0.0303 0.0113 0.0054 0.0184 0.0097 0.0132 0.0139 0.0023 0.0042 0.0092 0.0026 0.0034
Endosome 0.0082 0.0085 0.0226 0.0313 0.0846 0.021 0.1118 0.0036 0.0386 0.0243 0.0871 0.0058 0.0556 0.018 0.0117 0.0501 0.0249 0.0073
Golgi 0.0049 0.0041 0.0094 0.011 0.0024 0.0059 0.0212 0.0012 0.0091 0.0116 0.0477 0.001 0.0281 0.0055 0.015 0.0045 0.0047 0.004
Lipid Particles 0.0016 0.0005 0.0043 0.0002 0.0005 0.0064 0.0297 0.0002 0.0037 0.0092 0.0173 0.0001 0.0308 0.0003 0.0041 0.0011 0.0003 0.0001
Mitochondria 0.0009 0.007 0.01 0.0052 0.0005 0.0035 0.009 0.0008 0.0055 0.0412 0.1042 0.0004 0.0182 0.0002 0.0038 0.0018 0.0005 0.0009
None 0.0061 0.0073 0.0016 0.0018 0.0013 0.0016 0.0206 0.0024 0.0184 0.0038 0.0086 0.0012 0.0212 0.004 0.002 0.0397 0.0057 0.0287
Nuclear Periphery 0.0017 0.0008 0.0018 0.0013 0.001 0.0006 0.0247 0.0005 0.0068 0.0302 0.031 0.0006 0.0058 0.0004 0.0005 0.0032 0.0002 0.0002
Nucleolus 0.0001 0.0002 0.0001 0.0001 0 0 0.0028 0.0001 0.0006 0.0006 0.007 0 0.0003 0 0.0001 0.0002 0.0001 0
Nucleus 0.0018 0.0018 0.0015 0.0016 0.0009 0.001 0.0222 0.0012 0.0084 0.0288 0.0155 0.0008 0.0016 0.0006 0.0006 0.0023 0.0005 0.0003
Peroxisomes 0.0003 0.0004 0.0156 0.0001 0.0002 0.0004 0.0191 0.0004 0.0012 0.0053 0.0398 0.0002 0.0038 0.0006 0.024 0.001 0.0012 0.0004
Punctate Nuclear 0.0003 0.0002 0.0014 0.0001 0.0001 0.0001 0.0095 0.0003 0.0016 0.0011 0.0126 0.0002 0.0026 0.0006 0.0006 0.0014 0.0004 0.0001
Vacuole 0.0067 0.006 0.0132 0.0136 0.0221 0.0029 0.015 0.0015 0.0084 0.0085 0.0061 0.0009 0.0029 0.0057 0.0018 0.0095 0.0018 0.0009
Vacuole Periphery 0.0005 0.0008 0.0029 0.0029 0.0027 0.0007 0.004 0.0002 0.0022 0.0035 0.0085 0.0002 0.0017 0.0007 0.0004 0.0022 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.311 19.7169 23.7256 20.3912 31.4951 34.5554 22.293 25.8195 36.399 27.4931
Translational Efficiency 3.2186 1.9809 1.2213 2.424 1.7647 1.3883 1.1621 1.1187 1.0033 1.5055

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFR016C is not an essential gene
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Aip5

Aip5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aip5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available