Standard name
Human Ortholog
Description Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.82 0.89 0.85 0.8 0.72 0.72 0.7 0.57 0.58 0.55 0.59 0.63 0.66 0.79 – 0.64 0.57 0.63 0.63 0.63 0.74 0.73 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0 0 0 0.15 0.14 0.16 0.47 0.37 0.46 0.41 0 0 0 – 0.07 0 0.06 0 0.08 0 0 0.05
Nucleus 0.08 0.07 0 0.09 0.1 0.15 0.13 0.14 0.11 0.16 0.15 0.15 0.32 0.26 0.05 – 0.17 0.26 0.1 0.12 0.07 0.06 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.1 0.07 0.06 0.05 0.09 0.06 0.06 0.05 0 0 0 0 0.06 0.13 0.18 – 0.2 0.19 0.13 0.1 0.09 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 3 3 1 0 1
Bud 1 1 0 0 0 1 3 1 14 6 8 17 0 0 0 – 0 0 2 3 5 0 5 7
Bud Neck 0 0 10 3 4 3 12 8 10 11 10 9 0 0 0 – 0 0 1 1 1 1 0 1
Bud Site 1 0 0 0 0 0 1 0 5 2 4 5 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 3 0 1 0 1 3 1 4 2 0 0 0 0 – 0 0 0 0 1 1 0 0
Cytoplasm 163 326 313 287 305 331 504 425 353 352 340 442 53 148 187 – 87 42 139 275 240 128 131 171
Endoplasmic Reticulum 0 1 2 0 1 0 0 0 0 0 1 0 0 1 0 – 4 4 0 0 1 0 0 3
Endosome 0 0 2 4 4 6 5 6 1 0 2 2 0 0 6 – 5 0 2 4 3 2 1 6
Golgi 0 0 0 0 1 0 1 1 0 0 0 0 1 0 0 – 0 1 0 2 1 0 0 2
Mitochondria 7 45 0 11 10 69 97 95 288 227 285 310 0 0 1 – 9 2 13 12 30 5 4 11
Nucleus 16 26 15 30 39 71 90 87 70 95 94 114 27 58 11 – 23 19 21 53 27 10 9 6
Nuclear Periphery 0 1 1 0 1 0 1 1 1 3 1 0 0 0 0 – 1 0 0 0 0 1 0 0
Nucleolus 0 1 1 0 0 1 0 1 1 0 0 1 0 0 1 – 0 0 0 1 0 2 2 4
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
SpindlePole 0 0 2 0 6 4 19 14 7 14 8 20 0 0 0 – 2 0 6 20 15 3 7 7
Vac/Vac Membrane 20 29 20 16 36 28 44 31 12 15 17 25 5 29 43 – 28 14 28 42 35 7 7 15
Unique Cell Count 198 397 352 337 383 459 700 603 618 606 615 754 84 224 238 137 74 222 436 382 173 179 245
Labelled Cell Count 209 432 370 351 408 514 778 673 763 729 772 945 86 236 249 159 82 222 436 382 173 179 245


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.2 5.0 4.5 5.4 4.1 4.1 4.3 3.2 3.5 3.4 3.6 4.8 5.0 4.7 4.4 5.0 5.6 5.7 5.6 6.0
Std Deviation (1e-4) 0.5 0.7 1.4 1.5 2.4 1.3 1.4 1.4 0.9 1.0 0.8 0.8 1.3 1.0 1.4 1.7 1.8 1.7 2.7 2.4 2.7
Intensity Change (Log2) – – – -0.13 0.12 -0.3 -0.28 -0.21 -0.64 -0.49 -0.57 -0.47 -0.07 -0.0 -0.08 -0.17 0.01 0.16 0.2 0.18 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 4.9 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5989 1.0526 0.7679 0.4673 0.6398 0.6234 2.7724 2.6644 2.8006 2.9788 2.1611 2.4495 -0.4429 0.2152 0.0239 -0.2932 -0.7112 -0.0638
Actin 0.019 0.0087 0.0072 0.0096 0.045 0.0009 0.0332 0.0006 0.0079 0.0215 0.0031 0.0066 0.0205 0.0002 0.0008 0.0003 0.0002 0.0004
Bud 0.0032 0.0006 0.0014 0.0067 0.0002 0.0001 0.0002 0.0001 0.0005 0.0003 0.0002 0.0012 0.0004 0.0002 0.0003 0.0002 0.0009 0.0003
Bud Neck 0.0037 0.0006 0.0008 0.0012 0.0006 0.0013 0.0009 0.0004 0.0006 0.0431 0.0003 0.0018 0.001 0.0008 0.0005 0.0032 0.0002 0.0017
Bud Periphery 0.0122 0.0005 0.0007 0.0022 0.0005 0.0001 0.0002 0.0001 0.0008 0.0003 0.0005 0.0027 0.0009 0.0002 0.0009 0.0005 0.0011 0.0003
Bud Site 0.0094 0.0049 0.0028 0.0021 0.0005 0 0.0012 0.0024 0.0026 0.0028 0.0009 0.0003 0.0017 0.0072 0.0004 0.001 0.0001 0.0001
Cell Periphery 0.0009 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0004 0.0001 0
Cytoplasm 0.1017 0.1553 0.0959 0.0324 0.1537 0.1583 0.0546 0.1356 0.1193 0.0442 0.0419 0.1296 0.1202 0.161 0.1371 0.1464 0.1228 0.1785
Cytoplasmic Foci 0.0355 0.0059 0.0065 0.0233 0.0385 0.0166 0.0515 0.0054 0.0101 0.03 0.0105 0.0171 0.0055 0.0092 0.0096 0.0057 0.0068 0.0106
Eisosomes 0.0006 0.0003 0.0003 0.0001 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0039 0.0043 0.0018 0.0012 0.0038 0.002 0.0016 0.0078 0.0067 0.0006 0.0014 0.0035 0.0039 0.0061 0.0064 0.003 0.0015 0.0033
Endosome 0.0099 0.0021 0.0027 0.0199 0.0126 0.0021 0.0095 0.0052 0.0234 0.004 0.009 0.004 0.0058 0.0064 0.0036 0.006 0.0025 0.0023
Golgi 0.0037 0.0018 0.0012 0.0021 0.0119 0.0003 0.0081 0.0005 0.0053 0.0034 0.0075 0.0007 0.001 0.0005 0.0001 0.0006 0.0001 0.0001
Lipid Particles 0.013 0.001 0.0038 0.0103 0.0492 0.0059 0.0265 0.0021 0.0108 0.0126 0.043 0.0013 0.0009 0.0041 0.0005 0.0055 0.0004 0.0005
Mitochondria 0.0111 0.0062 0.0177 0.0012 0.0109 0.0004 0.0013 0.0004 0.0038 0.0004 0.0229 0.0023 0.0017 0.001 0.0008 0.0035 0.0004 0.0005
None 0.6207 0.6055 0.5915 0.8367 0.5408 0.7299 0.7586 0.6717 0.5979 0.7697 0.8343 0.703 0.7606 0.621 0.6325 0.7623 0.8553 0.6513
Nuclear Periphery 0.0084 0.0091 0.0073 0.0035 0.0051 0.007 0.0026 0.034 0.0109 0.0019 0.0008 0.0072 0.0061 0.0093 0.0098 0.0042 0.001 0.0045
Nucleolus 0.0105 0.0032 0.0055 0.0023 0.0011 0.0012 0.0007 0.0067 0.0019 0.0002 0.0019 0.002 0.0018 0.0022 0.002 0.007 0.0002 0.0017
Nucleus 0.0811 0.1666 0.2341 0.0103 0.0217 0.05 0.0081 0.1146 0.1668 0.0302 0.001 0.1005 0.0542 0.1578 0.1776 0.0383 0.0032 0.1264
Peroxisomes 0.0197 0.0038 0.0031 0.0039 0.0827 0.0015 0.0296 0.0002 0.0142 0.0245 0.0169 0.0014 0.0005 0.0008 0.0023 0.0026 0.0002 0.0002
Punctate Nuclear 0.0245 0.0168 0.0135 0.027 0.0187 0.0209 0.0096 0.009 0.0095 0.0096 0.0009 0.0122 0.0055 0.0075 0.0123 0.0023 0.0015 0.0149
Vacuole 0.0062 0.0023 0.0015 0.0034 0.0013 0.0013 0.0013 0.0021 0.0058 0.0002 0.0017 0.002 0.006 0.0036 0.002 0.0061 0.0013 0.0022
Vacuole Periphery 0.0011 0.0004 0.0003 0.0005 0.0006 0.0002 0.0003 0.0007 0.0009 0.0001 0.0007 0.0005 0.0008 0.0004 0.0004 0.0009 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.5649 3.4978 2.5955 6.8548 7.6096 7.8212 2.6525 3.9783 4.9839 9.0143
Translational Efficiency 4.2223 1.2072 1.4665 1.4739 2.0215 2.8148 2.2913 1.0645 1.5651 1.3139

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (24%), nucleus (7%), mixed (49%)
Cell Cycle Regulation Yes
cytoplasm - MA (2e-06)
Subcompartmental Group N/A

Eco1

Eco1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eco1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available