Standard name
Human Ortholog
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0 0 – 0 0 0.05 0.12 0.15 0.12 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0.05 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.35 0.56 0.33 0.59 0.5 0.59 0.75 – 0.74 0.79 0 0 0.14 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0.81 0.82 0.86 0.78 0.66 0.62 0.66 0.6 0.57 – 0.64 0.55 0.88 0.81 0.71 0.72 0.74 0.68 0.64 0.73 0.75 0.69 0.67 0.54
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0 0.09 0.18 0.17 0.15 0.17 0.16 – 0.13 0.17 0 0 0.05 0.07 0.11 0.13 0.18 0.09 0.08 0.07 0.05 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0.07 0.11 0 0.06 0.07 0.09 0.17 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 2 2 17 17 9 – 5 7 0 0 0 0 1 0 0 1 2 1 5 2
Bud Neck 0 0 1 0 0 0 1 2 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 2 16 17 29 14 – 11 21 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 7 6 7 16 25 21 22 13 – 8 6 14 41 57 24 13 13 0 2 1 0 1 2
Endoplasmic Reticulum 1 3 1 0 0 0 0 0 0 – 0 0 0 1 1 6 8 6 1 1 1 0 1 1
Endosome 8 2 0 0 8 3 4 1 2 – 1 1 2 0 2 3 7 10 15 6 1 7 5 16
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 0 0 0 1
Mitochondria 13 15 57 119 123 258 302 494 311 – 258 469 6 7 53 12 4 11 7 3 1 1 6 7
Nucleus 294 159 140 165 247 269 397 500 234 – 223 323 235 270 266 147 110 119 252 149 121 110 209 161
Nuclear Periphery 6 6 0 0 12 5 28 10 6 – 8 17 1 6 4 0 2 4 14 0 1 0 0 2
Nucleolus 44 18 7 20 67 74 89 141 66 – 45 100 5 14 20 15 16 22 69 19 12 11 14 21
Peroxisomes 1 0 0 0 1 0 1 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 5 0 1 2 13 10 21 26 7 – 2 8 1 1 0 2 5 2 14 4 3 5 8 5
Vac/Vac Membrane 12 9 2 0 4 3 12 16 1 – 4 8 14 12 13 9 10 20 10 12 11 14 52 72
Unique Cell Count 364 195 162 212 372 436 604 835 413 351 590 266 335 377 203 148 174 395 205 162 160 313 300
Labelled Cell Count 393 219 215 313 495 665 910 1258 663 565 961 278 353 417 219 176 207 395 205 162 160 313 300


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 5.9 4.3 4.1 4.4 4.0 4.0 3.8 3.3 – 3.4 3.1 6.0 6.1 5.5 8.0 8.6 8.9 5.9 5.8 6.2
Std Deviation (1e-4) 1.4 1.1 0.6 0.7 1.2 1.0 1.3 0.9 0.8 – 1.0 0.8 1.1 1.5 1.5 1.8 2.2 2.2 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.06 0.04 -0.1 -0.11 -0.17 -0.36 – -0.35 -0.47 0.48 0.52 0.35 0.91 1.01 1.06 0.45 0.43 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.3 1.0 -0.1 0 -0.3 – 0 0 0.7 3.0 3.8 2.8 1.9 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -9.3 -10.3 -5.6 -7.1 -7.2 -6.6
Nucleus -2.1 -4.8 -5.8 -5.1 -6.4 -6.7 – -5.3 -7.3 0.6 -1.6 -3.9 -3.2 -2.7 -3.9
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 -0.1 0.5 1.2 2.2 2.7
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 2.1 0 0 0 2.5 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8614 3.6023 3.7757 3.2805 3.4715 3.7861 3.2083 2.9834 3.1021 2.6843 2.6465 2.639 0.9851 1.1323 0.9192 0.8475 0.5554 0.8487
Actin 0 0.0001 0.0006 0.0001 0.0001 0.0001 0.0918 0.0002 0.0225 0.0024 0.0035 0.0008 0.0037 0.0002 0.0203 0.0002 0.0075 0.0001
Bud 0 0.0001 0.0002 0.0002 0.0001 0.0001 0.0007 0.0001 0.0008 0.0006 0.0005 0.0001 0.0003 0.0001 0.0003 0.0001 0.0004 0
Bud Neck 0.0002 0.0018 0.0027 0.0029 0.0186 0.0106 0.0065 0.0009 0.0012 0.0027 0.0128 0.0028 0.0006 0.0011 0.0022 0.0013 0.013 0.003
Bud Periphery 0 0.0001 0.0005 0.0003 0.0005 0.0002 0.001 0.0001 0.0009 0.0014 0.0018 0.0003 0.0002 0.0002 0.0008 0.0002 0.0019 0.0001
Bud Site 0.0001 0.0006 0.001 0.0009 0.0017 0.0003 0.0105 0.0009 0.0034 0.0026 0.001 0.0002 0.0009 0.0006 0.0035 0.0006 0.0018 0.0001
Cell Periphery 0 0 0.0003 0.0002 0.0003 0.0001 0.0005 0.0001 0.0002 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0
Cytoplasm 0.0004 0.004 0.0015 0.0006 0.0001 0.0004 0.0063 0.0088 0.0088 0.0012 0.0006 0.0003 0.0057 0.0213 0.0081 0.0046 0.0007 0.001
Cytoplasmic Foci 0 0.0001 0.0004 0.0005 0.0019 0.0005 0.0294 0.0007 0.0071 0.0081 0.0069 0.0007 0.0119 0.0017 0.0077 0.0007 0.005 0.0002
Eisosomes 0 0 0 0 0 0 0.001 0 0.0001 0 0.0002 0 0.0001 0 0.0003 0 0.0006 0
Endoplasmic Reticulum 0.0001 0.0003 0.0006 0.0004 0 0.0001 0.0062 0.001 0.0013 0.002 0.0013 0.0002 0.0046 0.0011 0.0019 0.0033 0.0006 0.0001
Endosome 0.0001 0.0002 0.0025 0.0008 0.0007 0.0006 0.0386 0.0011 0.0092 0.0166 0.0149 0.0036 0.0104 0.0015 0.0082 0.0043 0.0071 0.0003
Golgi 0 0 0.0004 0.0002 0.0006 0.0001 0.0129 0.0001 0.0056 0.0029 0.015 0.0028 0.0013 0.0002 0.003 0.0003 0.0038 0
Lipid Particles 0 0.0001 0.0009 0.0027 0.0056 0.0006 0.0491 0.0001 0.0046 0.0076 0.0361 0.0098 0.0049 0.0018 0.0119 0.0034 0.017 0.0006
Mitochondria 0.0002 0.0002 0.0008 0.0003 0.0003 0.0002 0.0053 0.0005 0.0064 0.0039 0.0217 0.013 0.0018 0.0004 0.0057 0.0003 0.0152 0.0003
None 0.0001 0.0004 0.0008 0.0005 0.0002 0.0001 0.0097 0.0002 0.001 0.0006 0.0008 0.0001 0.0057 0.0039 0.0186 0.0011 0.0021 0.0005
Nuclear Periphery 0.0075 0.0066 0.0277 0.035 0.0019 0.0073 0.0754 0.0267 0.0344 0.0385 0.0722 0.0153 0.0173 0.0596 0.0629 0.0375 0.0302 0.0094
Nucleolus 0.0349 0.092 0.1291 0.3705 0.4608 0.0952 0.0144 0.037 0.1266 0.2918 0.2704 0.1103 0.0395 0.1031 0.1357 0.2971 0.4047 0.0921
Nucleus 0.9499 0.8585 0.7952 0.4744 0.2584 0.8586 0.6039 0.8877 0.7088 0.5602 0.4103 0.7972 0.8708 0.7498 0.6419 0.5564 0.3333 0.8439
Peroxisomes 0 0 0.0002 0.0001 0.0004 0.0001 0.0139 0 0.0046 0.0126 0.0051 0.0007 0.0007 0.0005 0.0084 0.0001 0.0038 0
Punctate Nuclear 0.0061 0.0343 0.0312 0.107 0.2467 0.0242 0.0155 0.0312 0.0449 0.0376 0.1171 0.0404 0.0179 0.0511 0.0546 0.0856 0.1465 0.0475
Vacuole 0.0002 0.0004 0.0028 0.001 0.001 0.0006 0.0037 0.0016 0.005 0.004 0.004 0.0006 0.0012 0.0012 0.0019 0.0021 0.0023 0.0003
Vacuole Periphery 0.0001 0.0001 0.0008 0.0014 0.0001 0.0001 0.0037 0.0009 0.0027 0.0024 0.0036 0.0009 0.0005 0.0006 0.0016 0.0007 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1503 12.3543 9.7925 12.2609 13.2351 14.1263 14.4077 15.233 12.6024 15.8805
Translational Efficiency 0.7781 0.5965 0.4998 0.4498 0.4569 0.5563 0.4672 0.3232 0.4016 0.4348

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Smc2

Smc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available