Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 27 | 0 | 10 | 15 | 24 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 2 | 0 | 0 | 0 | 1 | 2 | 2 | 4 | 3 | 13 | 4 | 5 | 9 | 1 | 9 | 5 | 0 | 0 | 0 | 0 | 3 | 0 |
Cytoplasm | 247 | 384 | 59 | 51 | 41 | 74 | 117 | 146 | 74 | 96 | 93 | 130 | 290 | 404 | 471 | 180 | 187 | 138 | 223 | 342 | 62 | 255 | 310 | 359 |
Endoplasmic Reticulum | 3 | 5 | 0 | 0 | 0 | 4 | 0 | 17 | 9 | 7 | 4 | 11 | 2 | 3 | 5 | 6 | 17 | 5 | 3 | 6 | 0 | 5 | 4 | 5 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 1 | 2 | 3 | 0 | 0 | 1 | 1 | 8 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 3 | 11 | 11 | 10 | 11 | 0 | 0 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 3 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 1 | 0 | 2 | 6 | 8 | 15 | 8 | 8 | 6 | 9 | 0 | 1 | 0 | 2 | 5 | 7 | 1 | 8 | 0 | 4 | 6 | 4 |
Unique Cell Count | 250 | 393 | 59 | 52 | 42 | 84 | 129 | 185 | 100 | 127 | 113 | 166 | 292 | 407 | 477 | 185 | 202 | 148 | 239 | 399 | 66 | 286 | 352 | 408 |
Labelled Cell Count | 252 | 400 | 62 | 52 | 44 | 84 | 133 | 190 | 108 | 134 | 122 | 183 | 299 | 413 | 490 | 195 | 229 | 157 | 239 | 399 | 66 | 286 | 352 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 115.4 | 61.3 | 69.3 | 81.3 | 29.8 | 39.6 | 17.5 | 27.5 | 21.2 | 21.2 | 21.5 | 22.9 | 79.9 | 68.9 | 66.1 | 93.9 | 115.5 | 132.9 | 74.8 | 78.1 | 81.1 |
Std Deviation (1e-4) | 42.1 | 36.0 | 41.7 | 36.5 | 15.9 | 20.1 | 9.1 | 12.8 | 10.3 | 10.3 | 10.3 | 10.4 | 38.2 | 36.9 | 34.7 | 50.5 | 63.8 | 63.0 | 41.1 | 43.0 | 43.3 |
Intensity Change (Log2) | – | – | – | 0.23 | -1.22 | -0.81 | -1.98 | -1.33 | -1.71 | -1.71 | -1.69 | -1.6 | 0.2 | -0.01 | -0.07 | 0.44 | 0.74 | 0.94 | 0.11 | 0.17 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.1 | -1.2 | -2.7 | -2.4 | -3.8 | -4.3 | -4.2 | -3.4 | -3.9 | -0.6 | -0.7 | -0.9 | -1.3 | -2.2 | -2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 109.7584 | 128.7963 | 126.2251 | 109.158 | 88.8385 | 113.2454 | 111.0613 | 130.2648 | 125.1382 | 110.0053 | 94.6784 | 130.0419 | 102.47 | 126.2017 | 110.4482 | 110.1682 | 100.6615 | 115.6958 |
Actin | 0.0031 | 0.0006 | 0.0005 | 0.0003 | 0.0068 | 0.0034 | 0.0038 | 0.0003 | 0.0021 | 0.0009 | 0.0062 | 0.0011 | 0.0086 | 0.0002 | 0.0035 | 0.0009 | 0.049 | 0.0005 |
Bud | 0.0014 | 0.0008 | 0.0017 | 0.002 | 0.0051 | 0.0017 | 0.0046 | 0.0004 | 0.0021 | 0.0036 | 0.0062 | 0.0004 | 0.0017 | 0.0003 | 0.0011 | 0.0011 | 0.0016 | 0.0004 |
Bud Neck | 0.0011 | 0.0008 | 0.0009 | 0.0008 | 0.001 | 0.0028 | 0.0014 | 0.0005 | 0.0012 | 0.0009 | 0.0017 | 0.004 | 0.0011 | 0.0004 | 0.0012 | 0.0009 | 0.0005 | 0.0009 |
Bud Periphery | 0.0018 | 0.0007 | 0.0009 | 0.0011 | 0.0045 | 0.0017 | 0.0041 | 0.0002 | 0.0014 | 0.0024 | 0.0053 | 0.0001 | 0.0029 | 0.0001 | 0.0009 | 0.0008 | 0.0006 | 0.0002 |
Bud Site | 0.0014 | 0.0006 | 0.0006 | 0.0004 | 0.0033 | 0.0019 | 0.0024 | 0.0004 | 0.0017 | 0.001 | 0.0019 | 0.0001 | 0.001 | 0.0003 | 0.0007 | 0.0005 | 0.0009 | 0.0001 |
Cell Periphery | 0.0256 | 0.0147 | 0.0207 | 0.0055 | 0.0045 | 0.041 | 0.0384 | 0.0078 | 0.0182 | 0.0288 | 0.0125 | 0.0004 | 0.0346 | 0.0067 | 0.0156 | 0.0435 | 0.0018 | 0.0062 |
Cytoplasm | 0.6432 | 0.9046 | 0.7663 | 0.5789 | 0.7214 | 0.7136 | 0.6732 | 0.9522 | 0.8073 | 0.6205 | 0.6809 | 0.9066 | 0.6681 | 0.9547 | 0.8247 | 0.626 | 0.704 | 0.8911 |
Cytoplasmic Foci | 0.0045 | 0.0013 | 0.0015 | 0.0036 | 0.0187 | 0.0167 | 0.012 | 0.0008 | 0.0039 | 0.003 | 0.0123 | 0.0063 | 0.0153 | 0.0007 | 0.0074 | 0.01 | 0.0066 | 0.0024 |
Eisosomes | 0.0003 | 0 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.076 | 0.0261 | 0.0081 | 0.0122 | 0.0019 | 0.0559 | 0.0461 | 0.0087 | 0.0151 | 0.0041 | 0.0057 | 0.0121 | 0.0414 | 0.0079 | 0.009 | 0.0028 | 0.0119 | 0.0071 |
Endosome | 0.0119 | 0.003 | 0.0042 | 0.0066 | 0.0373 | 0.0318 | 0.0096 | 0.0014 | 0.0033 | 0.0044 | 0.0451 | 0.0439 | 0.0132 | 0.001 | 0.0124 | 0.0371 | 0.0512 | 0.0041 |
Golgi | 0.0029 | 0.0011 | 0.0003 | 0.0006 | 0.0177 | 0.0057 | 0.0017 | 0.0002 | 0.0004 | 0.0003 | 0.0068 | 0.0036 | 0.0051 | 0.0002 | 0.0036 | 0.0266 | 0.0121 | 0.0003 |
Lipid Particles | 0.0038 | 0.0021 | 0.0008 | 0.0023 | 0.0238 | 0.0076 | 0.0041 | 0.0005 | 0.0018 | 0.0037 | 0.0035 | 0.0005 | 0.013 | 0.0003 | 0.0071 | 0.0073 | 0.0041 | 0.0015 |
Mitochondria | 0.0084 | 0.0003 | 0.0004 | 0.0294 | 0.02 | 0.0038 | 0.0063 | 0.0001 | 0.0023 | 0.012 | 0.0135 | 0.0015 | 0.0114 | 0.0001 | 0.0062 | 0.0035 | 0.0023 | 0.0007 |
None | 0.0041 | 0.0001 | 0.0003 | 0.0052 | 0.0047 | 0.0028 | 0.0039 | 0.0002 | 0.0062 | 0.009 | 0.0044 | 0.0002 | 0.0057 | 0.0001 | 0.0113 | 0.0002 | 0.0011 | 0.0005 |
Nuclear Periphery | 0.0368 | 0.0048 | 0.0134 | 0.0057 | 0.0063 | 0.0136 | 0.0322 | 0.002 | 0.0082 | 0.0102 | 0.0085 | 0.0041 | 0.0319 | 0.0024 | 0.0061 | 0.0082 | 0.0141 | 0.0249 |
Nucleolus | 0.0026 | 0.0001 | 0.0005 | 0.0041 | 0.0266 | 0.0036 | 0.0031 | 0.0003 | 0.002 | 0.0061 | 0.0066 | 0 | 0.0026 | 0.0001 | 0.0064 | 0.0005 | 0.0004 | 0.0007 |
Nucleus | 0.0096 | 0.0014 | 0.006 | 0.006 | 0.0104 | 0.006 | 0.0082 | 0.0016 | 0.0058 | 0.0104 | 0.0135 | 0.002 | 0.0098 | 0.0013 | 0.0052 | 0.005 | 0.0049 | 0.0084 |
Peroxisomes | 0.0012 | 0 | 0.0001 | 0.0012 | 0.0121 | 0.0033 | 0.0006 | 0 | 0.0003 | 0.0005 | 0.0013 | 0.0001 | 0.0036 | 0 | 0.0042 | 0.0027 | 0.0016 | 0 |
Punctate Nuclear | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0034 | 0.0014 | 0.0012 | 0 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0036 | 0 | 0.0006 | 0.0002 | 0.0007 | 0.0002 |
Vacuole | 0.1238 | 0.0327 | 0.1451 | 0.2542 | 0.0502 | 0.066 | 0.1196 | 0.0203 | 0.099 | 0.2011 | 0.1298 | 0.0085 | 0.1025 | 0.0217 | 0.0575 | 0.1809 | 0.0978 | 0.039 |
Vacuole Periphery | 0.0358 | 0.004 | 0.0274 | 0.0794 | 0.0204 | 0.0154 | 0.0231 | 0.002 | 0.0172 | 0.0761 | 0.0332 | 0.0044 | 0.0225 | 0.0015 | 0.0151 | 0.0414 | 0.0326 | 0.0108 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2118.825 | 2320.8851 | 2539.2836 | 2307.9416 | 2723.7732 | 2098.9117 | 2111.0023 | 2164.1235 | 2360.1266 | 2004.1865 |
Translational Efficiency | 1.3798 | 1.2902 | 1.2771 | 1.1533 | 1.1848 | 1.2778 | 1.3346 | 1.3156 | 1.1342 | 1.3231 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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