Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 – 0 0 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.11 0.12 0.1 0.08 0.06 – 0.07 0.1 0.13 0.17 0.2 0.19 0.24 0.33 0.11 0.14 0.1 0 0.07 0 0.13 0.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.12 0 – 0 0 0 0 0 0 0.06 0.06 0.09 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.12 0.56 0.54 0.24 0.47 – 0.55 0.66 0.66 0.71 0.74 0 0 0 0.07 0.08 0.15 0 0 0 0 0.05 0.06
Nucleus 0.7 0.6 0.54 0.43 0.36 0.38 – 0.36 0.36 0.32 0.23 0.22 0.6 0.52 0.47 0.62 0.64 0.62 0.57 0.55 0.44 0.42 0.4 0.32
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.07 0.08 0.21 0.22 0.23 – 0.18 0.11 0.12 0.09 0.07 0.12 0.12 0.1 0.18 0.11 0.17 0.06 0.08 0.08 0 0 0.06
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.17 0.12 – 0.06 0 0.07 0.05 0 0 0 0 0.1 0.06 0.09 0.09 0.09 0.14 0.09 0.09 0.11
Vac/Vac Membrane 0.07 0.16 0 0 0.07 0.05 – 0.05 0.07 0.05 0.05 0 0.07 0.07 0.08 0.06 0.1 0.05 0.13 0.11 0.15 0.15 0.19 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 1
Bud 2 0 9 10 2 6 – 31 11 18 19 11 0 0 0 1 0 0 14 5 11 13 13 13
Bud Neck 0 0 1 0 1 3 – 0 3 3 0 3 0 0 0 0 0 0 5 0 2 2 5 3
Bud Site 0 1 3 15 9 29 – 28 14 32 21 27 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 58 21 61 37 28 36 – 63 62 79 80 107 56 80 124 26 30 25 24 15 21 36 30 31
Endoplasmic Reticulum 3 0 0 1 0 0 – 0 0 1 0 2 1 1 0 8 5 4 1 0 3 1 1 1
Endosome 8 7 3 12 41 12 – 13 1 9 2 4 10 20 24 20 9 13 21 6 21 8 13 12
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 1 1 4 1 2 2 0 2
Mitochondria 152 24 293 205 84 260 – 496 402 416 337 404 11 8 6 17 17 36 15 2 21 9 17 19
Nucleus 439 117 283 164 127 213 – 319 219 205 109 123 175 175 179 144 141 149 416 124 270 120 123 102
Nuclear Periphery 4 1 2 7 6 10 – 10 8 4 2 2 2 8 9 5 3 4 6 1 11 1 2 2
Nucleolus 18 14 42 81 76 130 – 158 66 78 43 37 36 41 37 42 24 41 46 16 49 12 10 20
Peroxisomes 0 0 0 0 5 3 – 1 0 1 1 1 0 1 4 0 0 0 0 0 0 0 0 1
SpindlePole 10 3 23 33 58 69 – 54 24 44 23 19 6 14 6 23 13 21 61 20 85 25 29 33
Vac/Vac Membrane 43 31 19 9 24 26 – 46 41 34 23 21 19 24 31 14 22 11 91 25 95 42 59 60
Unique Cell Count 631 196 523 383 350 556 894 610 631 475 547 290 334 380 234 219 239 728 226 616 286 313 320
Labelled Cell Count 738 219 739 575 461 797 1219 851 924 660 761 316 373 423 301 265 306 728 226 616 286 313 320


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.2 3.8 4.0 4.9 4.0 – 3.8 3.4 3.6 3.5 3.1 5.3 5.7 5.5 7.4 7.1 7.0 5.3 5.3 5.1
Std Deviation (1e-4) 0.8 1.1 0.9 1.6 1.5 1.1 – 1.2 1.1 1.2 1.2 0.7 1.2 1.4 1.5 1.9 1.9 1.9 1.4 1.2 1.6
Intensity Change (Log2) – – – 0.06 0.37 0.07 – 0.01 -0.15 -0.1 -0.14 -0.28 0.49 0.58 0.53 0.96 0.91 0.87 0.49 0.48 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -1.8 -3.0 – -3.0 -0.8 0.4 2.3 3.5 3.0 4.7 7.7 -0.2 0.8 -0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 7.4 0 – 0 0 0 0 0 0 4.8 5.0 5.9 0 4.3
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -9.4 0 – 0 0 0 0 0 -14.7 -16.0 -17.2 -12.6 -12.2 -10.6
Nucleus -3.4 -5.2 -5.2 – -6.8 -6.2 -7.4 -10.1 -10.7 1.7 -0.5 -2.1 1.9 2.6 2.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 5.8 0 – 0 0 0 0 0 2.0 2.0 0.9 4.0 1.3 3.8
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 6.1 0 – 0 0 0 0 0 -1.7 -0.1 -2.4 2.9 0.9 2.4
Vacuole -1.1 2.2 0.9 – 1.3 2.3 1.4 1.0 0.2 1.9 2.3 2.9 1.5 3.5 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0977 1.7018 1.7434 1.0248 1.6973 1.5008 0.7216 0.6208 0.7591 0.5187 1.5625 0.767 1.3553 2.3267 1.8923 2.074 1.6132 1.9018
Actin 0.0261 0.0002 0.0187 0.0002 0.0324 0.0065 0.0081 0.0001 0.0159 0.001 0.0076 0.0023 0.0021 0.0001 0.0002 0 0.0001 0.0003
Bud 0.0008 0.0002 0.0004 0.0001 0.0012 0.0002 0.0002 0.0001 0.0023 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0006 0.0001
Bud Neck 0.0045 0.0007 0.0062 0.0013 0.0015 0.0043 0.0014 0.0021 0.0013 0.0006 0.0022 0.0025 0.0008 0.0006 0.001 0.0002 0.0005 0.0031
Bud Periphery 0.0014 0.0001 0.0007 0.0001 0.0031 0.0003 0.0005 0.0001 0.0009 0.0001 0.001 0.0002 0.0002 0.0003 0.0001 0 0.001 0.0001
Bud Site 0.005 0.0008 0.0051 0.0002 0.0059 0.0002 0.0007 0.0005 0.0033 0.0009 0.0009 0.0002 0.0003 0.0007 0.0004 0.0001 0.0002 0.0001
Cell Periphery 0.0003 0 0.0008 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0002 0 0 0.0001 0 0 0 0
Cytoplasm 0.0234 0.0314 0.0107 0.0205 0.0009 0.0298 0.0218 0.0079 0.0051 0.0127 0.0046 0.0187 0.0153 0.0295 0.0127 0.0027 0.0079 0.0201
Cytoplasmic Foci 0.0259 0.0015 0.0057 0.0009 0.0029 0.0013 0.0114 0.0012 0.005 0.0065 0.0038 0.003 0.0012 0.0018 0.0022 0 0.001 0.0012
Eisosomes 0.0002 0 0.0006 0 0.0003 0 0.0003 0 0.0005 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0046 0.0021 0.002 0.0017 0.0014 0.0015 0.0056 0.0006 0.0014 0.0087 0.0039 0.0007 0.0012 0.0017 0.0006 0.0001 0.0004 0.0005
Endosome 0.0314 0.0024 0.007 0.0023 0.0077 0.0063 0.0164 0.001 0.0043 0.0219 0.0228 0.0049 0.0015 0.0009 0.0013 0 0.0004 0.0012
Golgi 0.0095 0.0001 0.003 0.0001 0.0041 0.0017 0.0033 0.0001 0.0021 0.007 0.0049 0.0026 0.0001 0.0001 0.0001 0 0 0
Lipid Particles 0.0182 0.0009 0.0071 0.0004 0.0051 0.0012 0.0174 0.0004 0.0048 0.0011 0.0221 0.0054 0.0005 0.0004 0.0004 0 0.0001 0.0001
Mitochondria 0.0084 0.0005 0.0021 0.0004 0.0095 0.0008 0.0074 0.0002 0.003 0.0081 0.0065 0.0031 0.0012 0.0003 0.0002 0.0002 0.0008 0.0002
None 0.0216 0.019 0.027 0.0042 0.0009 0.0137 0.0321 0.0106 0.0233 0.0073 0.0748 0.0234 0.0423 0.0812 0.0142 0.0021 0.0815 0.01
Nuclear Periphery 0.0404 0.0178 0.0439 0.0273 0.0345 0.0123 0.0554 0.0201 0.0445 0.0178 0.0724 0.0092 0.0193 0.0097 0.0276 0.0031 0.0031 0.0083
Nucleolus 0.0279 0.0354 0.047 0.0194 0.0727 0.053 0.0518 0.0695 0.0735 0.0168 0.0798 0.0712 0.0387 0.0448 0.0424 0.0085 0.0286 0.046
Nucleus 0.5887 0.7435 0.6299 0.8378 0.7383 0.7913 0.5627 0.7204 0.6309 0.7461 0.6144 0.7544 0.6697 0.6732 0.6202 0.9734 0.6723 0.7981
Peroxisomes 0.0167 0.0001 0.0047 0.0001 0.0029 0.0003 0.0052 0.0001 0.0048 0.0018 0.0125 0.0031 0.0001 0.0001 0.0002 0 0.0001 0.0001
Punctate Nuclear 0.1375 0.1404 0.1752 0.0808 0.0673 0.0726 0.1923 0.164 0.1711 0.1395 0.059 0.0939 0.203 0.1527 0.2748 0.0093 0.2004 0.1097
Vacuole 0.0049 0.0025 0.0014 0.0016 0.0031 0.0022 0.0039 0.0008 0.0012 0.0012 0.0031 0.0006 0.0015 0.0016 0.0009 0.0001 0.0007 0.0006
Vacuole Periphery 0.0026 0.0004 0.0009 0.0005 0.0042 0.0004 0.0021 0.0002 0.0007 0.0007 0.0033 0.0003 0.0006 0.0003 0.0002 0 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2409 13.1517 1.9518 8.327 13.3524 17.6447 6.6488 8.975 7.0273 10.3246
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cdc26

Cdc26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available