Standard name | |
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Human Ortholog | |
Description | Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 3 | 2 | 0 | 1 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 0 | 2 | 5 | 1 | 10 | – | 0 | 3 | 0 | 2 | 0 | 3 | 14 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 2 | – | – | – | 1 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 1 | 1 | 2 | – | 0 | 0 | 10 | 4 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 131 | 136 | 39 | 116 | 168 | 199 | 200 | – | 62 | 81 | 49 | 105 | 11 | 51 | 73 | – | – | – | 112 | 128 | 54 | 12 | 31 | 60 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 73 | 50 | 27 | 48 | 41 | 78 | 93 | – | 62 | 80 | 72 | 104 | 8 | 42 | 43 | – | – | – | 32 | 26 | 19 | 7 | 12 | 22 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 1 | 3 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 3 | 4 | 0 | 3 | – | 0 | 1 | 1 | 0 | 1 | 0 | 3 | – | – | – | 2 | 3 | 3 | 0 | 0 | 2 |
Unique Cell Count | 149 | 155 | 51 | 142 | 201 | 241 | 268 | 91 | 130 | 97 | 158 | 16 | 79 | 127 | 152 | 162 | 81 | 22 | 50 | 91 | ||||
Labelled Cell Count | 205 | 190 | 67 | 171 | 222 | 282 | 311 | 124 | 169 | 137 | 220 | 20 | 98 | 144 | 152 | 162 | 81 | 22 | 50 | 91 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.9 | 23.6 | 15.4 | 16.8 | 16.8 | 14.9 | 14.3 | – | 11.7 | 11.6 | 10.4 | 11.9 | 18.0 | 17.2 | 17.7 | – | – | – | – | 10.9 | 13.7 |
Std Deviation (1e-4) | 4.7 | 5.7 | 4.5 | 4.4 | 4.7 | 3.9 | 4.1 | – | 4.9 | 3.3 | 2.9 | 4.1 | 6.3 | 5.8 | 6.0 | – | – | – | – | 4.3 | 4.3 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.12 | -0.05 | -0.11 | – | -0.4 | -0.41 | -0.57 | -0.37 | 0.22 | 0.16 | 0.2 | – | – | – | – | -0.5 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0.8 | 1.2 | 1.0 | -0.3 | – | -1.1 | -1.8 | -3.1 | -1.3 | -0.6 | -1.4 | -2.4 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | -2.4 | -4.7 | -2.8 | -2.5 | – | 1.8 | 1.1 | 2.6 | 1.7 | -0.2 | 0 | -2.4 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.543 | 14.5381 | 13.5536 | 13.0076 | 14.1809 | 13.7022 | 15.6357 | 15.0166 | 14.6133 | 13.3777 | 13.3496 | 14.1408 | 14.5158 | 14.3423 | 13.5206 | 11.9967 | 11.489 | 13.5823 |
Actin | 0.0107 | 0 | 0.0065 | 0 | 0 | 0 | 0.0101 | 0 | 0.0137 | 0.0108 | 0.0078 | 0.0013 | 0.0009 | 0 | 0.0078 | 0 | 0.0339 | 0.0042 |
Bud | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 |
Bud Neck | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0.0002 |
Bud Periphery | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0009 | 0.0004 |
Bud Site | 0.0017 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0006 | 0 | 0.0016 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.001 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0033 | 0 | 0.0012 | 0.0003 | 0.0018 | 0.0002 | 0.0007 | 0 | 0.001 | 0 | 0.0024 | 0 |
Cytoplasmic Foci | 0.0194 | 0 | 0.0033 | 0.0043 | 0 | 0.0065 | 0.0064 | 0 | 0.0016 | 0.0138 | 0.0032 | 0.0024 | 0.0063 | 0 | 0.0005 | 0 | 0.01 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0014 | 0 | 0.0008 | 0.0002 | 0.0023 | 0.0009 | 0.0001 | 0 | 0.0001 | 0 | 0.0059 | 0.0002 |
Endosome | 0.0063 | 0 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0047 | 0 | 0.0022 | 0.0051 | 0.0209 | 0.0102 | 0.0005 | 0 | 0.0007 | 0 | 0.0565 | 0.0022 |
Golgi | 0.0015 | 0 | 0.001 | 0.0001 | 0 | 0.0001 | 0.001 | 0 | 0.001 | 0.0046 | 0.0028 | 0.0031 | 0.0001 | 0 | 0.0014 | 0 | 0.0277 | 0.0023 |
Lipid Particles | 0.0053 | 0 | 0.0019 | 0.0082 | 0.0001 | 0.0011 | 0.0045 | 0 | 0.0016 | 0.0097 | 0.0045 | 0.0011 | 0.0008 | 0 | 0.0004 | 0 | 0.0095 | 0.0041 |
Mitochondria | 0.0021 | 0.0002 | 0.0212 | 0.0041 | 0.0025 | 0.0002 | 0.0013 | 0.0002 | 0.0016 | 0.0025 | 0.0064 | 0.0041 | 0.0053 | 0.0004 | 0.0097 | 0.0007 | 0.0132 | 0.0121 |
None | 0.0013 | 0 | 0.001 | 0.0003 | 0 | 0.0001 | 0.0194 | 0 | 0.0006 | 0.0006 | 0.0011 | 0.0001 | 0.0009 | 0 | 0.0007 | 0 | 0.0007 | 0.0002 |
Nuclear Periphery | 0.0086 | 0.0055 | 0.0011 | 0.0005 | 0.0002 | 0.0002 | 0.0115 | 0.0001 | 0.014 | 0.0011 | 0.0596 | 0.0023 | 0.0056 | 0 | 0.0013 | 0.0001 | 0.0203 | 0.0014 |
Nucleolus | 0.1038 | 0.0568 | 0.0527 | 0.0959 | 0.5317 | 0.2599 | 0.0788 | 0.0534 | 0.0758 | 0.0263 | 0.552 | 0.2059 | 0.1104 | 0.0751 | 0.0423 | 0.0729 | 0.3774 | 0.184 |
Nucleus | 0.8131 | 0.9291 | 0.8993 | 0.8821 | 0.4632 | 0.7255 | 0.8246 | 0.9462 | 0.8632 | 0.8904 | 0.3175 | 0.7539 | 0.8269 | 0.9243 | 0.9266 | 0.9251 | 0.4253 | 0.7858 |
Peroxisomes | 0.0116 | 0 | 0.0058 | 0.0003 | 0 | 0 | 0.0037 | 0 | 0.006 | 0.0262 | 0.0015 | 0.0009 | 0.0002 | 0 | 0.0008 | 0 | 0.0034 | 0.0009 |
Punctate Nuclear | 0.0112 | 0.008 | 0.0028 | 0.001 | 0.0002 | 0.0054 | 0.0268 | 0.0001 | 0.0152 | 0.0064 | 0.014 | 0.0114 | 0.0392 | 0 | 0.0054 | 0 | 0.0065 | 0.0003 |
Vacuole | 0.0004 | 0 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0005 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0013 | 0.0002 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0005 | 0.002 | 0.0011 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0021 | 0.0012 | 0.0006 | 0 | 0.0001 | 0.0004 | 0.0019 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.5147 | 52.4417 | 54.2173 | 55.8131 | 64.5228 | 54.0198 | 58.8326 | 63.1307 | 67.7965 | 58.7326 |
Translational Efficiency | 2.5074 | 2.0732 | 1.7691 | 1.8975 | 1.756 | 1.7888 | 1.6175 | 1.4715 | 1.3097 | 1.5586 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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