Standard name
Human Ortholog
Description Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.24 0.22 0.2 0.22 0.14 0.14 0.12 0.16 0.13 0.07 0.1 0.36 0.4 0.38 0.05 0.07 0.07 0.07 0.08 0 0.11 0.17 0.13
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0.07
Endosome 0 0.17 0.1 0.1 0.22 0.07 0.11 0.17 0.05 0.05 0.05 0 0.12 0.17 0.19 0.41 0.35 0.29 0.19 0.19 0.19 0.23 0.22 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.69 0.23 0.52 0.6 0.39 0.68 0.65 0.57 0.69 0.74 0.77 0.79 0.09 0.08 0.08 0.07 0.07 0.14 0.12 0.15 0.14 0.1 0.13 0.18
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.11 0.2 0.14 0.1 0.14 0.16 0.16 0.07 0.08 0.07 0.13 0.09 0.24 0.23 0.24 0.1 0.07 0.13 0.37 0.36 0.3 0.3 0.16 0.13
Nucleolus 0.07 0 0.05 0.06 0 0.09 0.07 0.09 0.06 0.1 0.09 0.06 0 0 0.06 0.22 0.19 0.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0.26 0.19 0.2 0.05 0 0.07 0.05 0.09 0.06
SpindlePole 0 0 0.05 0.14 0 0 0 0.09 0.06 0.06 0 0 0 0 0 0.28 0.31 0.24 0.09 0.07 0.09 0.06 0.05 0
Vac/Vac Membrane 0.21 0.36 0.11 0.07 0.16 0.08 0.08 0.1 0.1 0.09 0.09 0.09 0.3 0.21 0.27 0.2 0.24 0.26 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 1 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 7 7 1 12 12
Bud 0 2 1 4 2 5 9 14 17 19 12 11 0 0 0 2 1 1 0 12 3 0 4 4
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 4 2 0 0 3
Bud Site 0 2 2 0 0 6 3 8 9 9 6 8 1 0 0 1 1 0
Cell Periphery 0 1 0 1 0 0 0 2 1 0 0 1 0 1 1 1 0 1 0 0 0 0 0 0
Cytoplasm 21 87 45 38 42 42 57 65 47 58 15 32 65 99 94 9 8 7 8 36 8 8 46 30
Endoplasmic Reticulum 1 13 10 1 4 4 2 6 3 3 8 4 11 3 0 1 3 4 4 8 7 5 12 14
Endosome 5 62 21 20 41 21 42 89 16 21 11 9 21 43 46 78 37 27 22 82 47 18 60 45
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 2 3 1 1 2 3 2 5 8
Mitochondria 81 85 107 115 72 204 257 299 207 319 167 261 17 21 19 13 8 13 14 63 33 7 34 40
Nucleus 2 3 2 3 1 4 3 15 6 8 2 4 0 0 4 4 3 2 0 1 1 0 0 0
Nuclear Periphery 13 72 29 19 27 48 64 37 25 29 28 28 43 57 59 18 7 12 44 155 71 24 43 29
Nucleolus 8 7 11 12 7 26 29 46 19 44 19 21 6 8 14 41 20 19 1 1 1 0 0 0
Peroxisomes 0 12 8 14 4 6 11 24 2 4 2 3 1 2 3 48 20 19 5 6 16 3 23 12
SpindlePole 4 4 10 27 6 10 17 49 18 25 3 8 5 7 7 52 33 23 10 29 20 5 14 6
Vac/Vac Membrane 25 129 23 13 30 25 33 54 31 39 19 31 54 53 66 37 26 24 3 15 6 1 4 11
Unique Cell Count 117 363 205 193 187 300 396 529 302 433 217 329 181 249 248 188 107 94 121 435 243 80 269 227
Labelled Cell Count 160 483 270 267 237 401 527 709 402 578 293 422 226 294 313 307 171 153 121 435 243 80 269 227


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.5 4.9 4.5 4.9 4.5 4.2 4.7 4.3 4.1 4.0 4.0 5.7 5.5 5.5 8.2 10.6 10.2 5.7 5.3 6.4
Std Deviation (1e-4) 1.5 1.4 1.4 1.4 1.7 1.6 1.4 1.4 1.6 1.7 1.0 1.1 1.5 1.6 1.7 1.9 7.4 2.5 2.0 1.7 1.8
Intensity Change (Log2) -0.12 0.0 -0.12 -0.22 -0.05 -0.18 -0.27 -0.29 -0.29 0.23 0.18 0.17 0.74 1.12 1.06 0.22 0.12 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 0.1 -2.3 -2.3 -3.3 -1.8 -2.7 -4.4 -3.9 3.0 4.1 3.7 -4.9 -3.2 -3.1
Endoplasmic Reticulum 0 -1.5 0 0 0 0 0 0 0 0.5 -2.3 0 -2.6 0 0
Endosome 0 3.2 0 0 0 0 0 0 0 0.4 2.1 2.5 7.1 5.2 4.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -2.7 0 0 0 0 0 0 0 -9.0 -10.3 -10.5 -9.7 -7.8 -6.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.1 0 0 0 0 0 0 0 2.4 2.4 2.6 -1.4 -2.0 -0.3
Nucleolus 0 -0.8 0 0 0 0 0 0 0 -1.0 -1.1 0.1 4.8 3.7 4.0
Peroxisomes 0 -1.0 0 0 0 0 0 0 0 0 0 0 6.1 4.3 4.6
SpindlePole 0 -0.8 0 0 0 0 0 0 0 -1.1 -1.2 -1.1 6.2 6.3 5.0
Vacuole -1.6 1.4 -1.1 -1.2 -0.4 -0.3 -0.9 -0.8 -0.7 4.6 2.9 4.1 2.3 3.0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5083 0.1845 -0.005 0.0281 -1.1422 0.1475 1.6104 1.5151 1.4032 1.4299 2.0828 1.9419 2.1691 1.8868 1.7 2.1781 2.2816 2.0846
Actin 0.0632 0.0116 0.0172 0.0011 0.0008 0.0133 0.0107 0.0008 0.0054 0.0083 0.0205 0.0103 0.031 0.0017 0.0164 0.0005 0.0429 0.002
Bud 0.0009 0.0003 0.0002 0 0.0001 0.0001 0.0005 0.0002 0.0002 0.0017 0.0002 0.0002 0.0002 0.0001 0.0046 0 0.0008 0.0001
Bud Neck 0.0063 0.0055 0.0005 0.0001 0.0006 0.0256 0.0031 0.0003 0.0007 0.0006 0.0039 0.0107 0.0009 0.0004 0.0036 0.0022 0.0023 0.0129
Bud Periphery 0.0021 0.0005 0.0007 0.0001 0.0001 0.0005 0.0013 0.0004 0.0008 0.0041 0.0004 0.0007 0.0005 0.0001 0.0042 0 0.0011 0.0017
Bud Site 0.025 0.0111 0.0007 0.0001 0.0004 0.0003 0.0035 0.0017 0.0006 0.0023 0.0014 0.0004 0.0012 0.0012 0.0089 0 0.0201 0.0001
Cell Periphery 0.0019 0.0008 0.0005 0.0001 0.0001 0.0004 0.0004 0.0002 0.0002 0.0003 0.0002 0.0001 0.0004 0.0003 0.001 0.0001 0.0003 0.0002
Cytoplasm 0.034 0.041 0.0646 0.0673 0.0895 0.0544 0.0395 0.0448 0.0433 0.0385 0.0424 0.0439 0.0356 0.0833 0.0688 0.0535 0.0169 0.0968
Cytoplasmic Foci 0.099 0.0344 0.0504 0.0454 0.025 0.0512 0.0615 0.0498 0.0533 0.0278 0.0622 0.0438 0.0819 0.0606 0.0578 0.0611 0.0086 0.0308
Eisosomes 0.0019 0.0008 0.0003 0.0002 0.0001 0.0005 0.0004 0.0001 0.0003 0.0004 0.0004 0.0002 0.0011 0.0004 0.0006 0.0003 0.0005 0.0003
Endoplasmic Reticulum 0.0152 0.049 0.036 0.0102 0.0136 0.0251 0.0171 0.0225 0.0137 0.0093 0.0186 0.0118 0.0244 0.0246 0.0241 0.0067 0.074 0.0237
Endosome 0.0273 0.0384 0.0395 0.0081 0.0745 0.0367 0.0493 0.0493 0.0546 0.0393 0.0527 0.0405 0.0671 0.0476 0.034 0.009 0.006 0.0248
Golgi 0.0159 0.0048 0.0051 0.0052 0.0051 0.012 0.0085 0.0036 0.0053 0.006 0.0184 0.005 0.013 0.0039 0.006 0.0021 0.0054 0.0031
Lipid Particles 0.2368 0.1686 0.1333 0.1363 0.0621 0.1401 0.1713 0.1153 0.1104 0.0973 0.1578 0.1336 0.1874 0.1139 0.1108 0.0656 0.069 0.0806
Mitochondria 0.0104 0.0014 0.0195 0.0016 0.0021 0.0327 0.0145 0.0023 0.0052 0.0122 0.0075 0.008 0.0063 0.0014 0.0047 0.0005 0.001 0.003
None 0.2388 0.3781 0.4405 0.658 0.5889 0.2941 0.3629 0.4306 0.4123 0.5877 0.3681 0.432 0.3726 0.4907 0.5465 0.7 0.6616 0.5679
Nuclear Periphery 0.124 0.2137 0.1368 0.0357 0.1085 0.2778 0.1727 0.1516 0.2031 0.1157 0.1296 0.1786 0.1302 0.1055 0.0496 0.0509 0.0799 0.0971
Nucleolus 0.0025 0.0017 0.0014 0.0006 0.0009 0.0005 0.0022 0.0049 0.0021 0.0016 0.0035 0.0022 0.0007 0.0022 0.0015 0.0005 0.0002 0.0011
Nucleus 0.0072 0.0043 0.0081 0.0022 0.0048 0.0038 0.006 0.0204 0.0071 0.0051 0.0085 0.0093 0.0021 0.0091 0.0033 0.0014 0.0006 0.013
Peroxisomes 0.0175 0.0029 0.0073 0.0059 0.0017 0.004 0.0236 0.007 0.0102 0.0108 0.0366 0.0128 0.0221 0.0025 0.0224 0.0182 0.0036 0.0053
Punctate Nuclear 0.0532 0.0115 0.0231 0.0176 0.0112 0.0151 0.0381 0.082 0.06 0.0207 0.0574 0.0456 0.0097 0.0387 0.0243 0.0249 0.0019 0.0295
Vacuole 0.0051 0.0073 0.0056 0.0019 0.0041 0.0027 0.0061 0.0064 0.0038 0.0041 0.0042 0.0039 0.0056 0.0062 0.0042 0.0015 0.001 0.0025
Vacuole Periphery 0.012 0.0124 0.0087 0.0025 0.0056 0.0091 0.0068 0.0059 0.0073 0.0062 0.0054 0.0064 0.0062 0.0057 0.0027 0.0009 0.0024 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0739 11.5817 21.9628 23.6027 20.064 17.2649 25.3722 32.0536 27.7334 25.2559
Translational Efficiency 0.7289 1.2173 0.8381 0.7152 0.5949 0.7467 0.6384 0.6263 0.6666 0.6183

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly
Localization
Cell Percentages nuclear periphery (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Osw7

Osw7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Osw7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available