Standard name | |
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Human Ortholog | |
Description | Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 7 | 7 | 1 | 12 | 12 |
Bud | 0 | 2 | 1 | 4 | 2 | 5 | 9 | 14 | 17 | 19 | 12 | 11 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 12 | 3 | 0 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 3 |
Bud Site | 0 | 2 | 2 | 0 | 0 | 6 | 3 | 8 | 9 | 9 | 6 | 8 | 1 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 21 | 87 | 45 | 38 | 42 | 42 | 57 | 65 | 47 | 58 | 15 | 32 | 65 | 99 | 94 | 9 | 8 | 7 | 8 | 36 | 8 | 8 | 46 | 30 |
Endoplasmic Reticulum | 1 | 13 | 10 | 1 | 4 | 4 | 2 | 6 | 3 | 3 | 8 | 4 | 11 | 3 | 0 | 1 | 3 | 4 | 4 | 8 | 7 | 5 | 12 | 14 |
Endosome | 5 | 62 | 21 | 20 | 41 | 21 | 42 | 89 | 16 | 21 | 11 | 9 | 21 | 43 | 46 | 78 | 37 | 27 | 22 | 82 | 47 | 18 | 60 | 45 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 2 | 3 | 2 | 5 | 8 |
Mitochondria | 81 | 85 | 107 | 115 | 72 | 204 | 257 | 299 | 207 | 319 | 167 | 261 | 17 | 21 | 19 | 13 | 8 | 13 | 14 | 63 | 33 | 7 | 34 | 40 |
Nucleus | 2 | 3 | 2 | 3 | 1 | 4 | 3 | 15 | 6 | 8 | 2 | 4 | 0 | 0 | 4 | 4 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 13 | 72 | 29 | 19 | 27 | 48 | 64 | 37 | 25 | 29 | 28 | 28 | 43 | 57 | 59 | 18 | 7 | 12 | 44 | 155 | 71 | 24 | 43 | 29 |
Nucleolus | 8 | 7 | 11 | 12 | 7 | 26 | 29 | 46 | 19 | 44 | 19 | 21 | 6 | 8 | 14 | 41 | 20 | 19 | 1 | 1 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 12 | 8 | 14 | 4 | 6 | 11 | 24 | 2 | 4 | 2 | 3 | 1 | 2 | 3 | 48 | 20 | 19 | 5 | 6 | 16 | 3 | 23 | 12 |
SpindlePole | 4 | 4 | 10 | 27 | 6 | 10 | 17 | 49 | 18 | 25 | 3 | 8 | 5 | 7 | 7 | 52 | 33 | 23 | 10 | 29 | 20 | 5 | 14 | 6 |
Vac/Vac Membrane | 25 | 129 | 23 | 13 | 30 | 25 | 33 | 54 | 31 | 39 | 19 | 31 | 54 | 53 | 66 | 37 | 26 | 24 | 3 | 15 | 6 | 1 | 4 | 11 |
Unique Cell Count | 117 | 363 | 205 | 193 | 187 | 300 | 396 | 529 | 302 | 433 | 217 | 329 | 181 | 249 | 248 | 188 | 107 | 94 | 121 | 435 | 243 | 80 | 269 | 227 |
Labelled Cell Count | 160 | 483 | 270 | 267 | 237 | 401 | 527 | 709 | 402 | 578 | 293 | 422 | 226 | 294 | 313 | 307 | 171 | 153 | 121 | 435 | 243 | 80 | 269 | 227 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.5 | 4.9 | 4.5 | 4.9 | 4.5 | 4.2 | 4.7 | 4.3 | 4.1 | 4.0 | 4.0 | 5.7 | 5.5 | 5.5 | 8.2 | 10.6 | 10.2 | 5.7 | 5.3 | 6.4 |
Std Deviation (1e-4) | 1.5 | 1.4 | 1.4 | 1.4 | 1.7 | 1.6 | 1.4 | 1.4 | 1.6 | 1.7 | 1.0 | 1.1 | 1.5 | 1.6 | 1.7 | 1.9 | 7.4 | 2.5 | 2.0 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.0 | -0.12 | -0.22 | -0.05 | -0.18 | -0.27 | -0.29 | -0.29 | 0.23 | 0.18 | 0.17 | 0.74 | 1.12 | 1.06 | 0.22 | 0.12 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0.1 | -2.3 | -2.3 | -3.3 | -1.8 | -2.7 | -4.4 | -3.9 | 3.0 | 4.1 | 3.7 | -4.9 | -3.2 | -3.1 |
Endoplasmic Reticulum | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | -2.3 | 0 | -2.6 | 0 | 0 |
Endosome | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 2.1 | 2.5 | 7.1 | 5.2 | 4.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -9.0 | -10.3 | -10.5 | -9.7 | -7.8 | -6.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.4 | 2.6 | -1.4 | -2.0 | -0.3 |
Nucleolus | 0 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -1.1 | 0.1 | 4.8 | 3.7 | 4.0 |
Peroxisomes | 0 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 4.3 | 4.6 |
SpindlePole | 0 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -1.2 | -1.1 | 6.2 | 6.3 | 5.0 |
Vacuole | -1.6 | 1.4 | -1.1 | -1.2 | -0.4 | -0.3 | -0.9 | -0.8 | -0.7 | 4.6 | 2.9 | 4.1 | 2.3 | 3.0 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5083 | 0.1845 | -0.005 | 0.0281 | -1.1422 | 0.1475 | 1.6104 | 1.5151 | 1.4032 | 1.4299 | 2.0828 | 1.9419 | 2.1691 | 1.8868 | 1.7 | 2.1781 | 2.2816 | 2.0846 |
Actin | 0.0632 | 0.0116 | 0.0172 | 0.0011 | 0.0008 | 0.0133 | 0.0107 | 0.0008 | 0.0054 | 0.0083 | 0.0205 | 0.0103 | 0.031 | 0.0017 | 0.0164 | 0.0005 | 0.0429 | 0.002 |
Bud | 0.0009 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0046 | 0 | 0.0008 | 0.0001 |
Bud Neck | 0.0063 | 0.0055 | 0.0005 | 0.0001 | 0.0006 | 0.0256 | 0.0031 | 0.0003 | 0.0007 | 0.0006 | 0.0039 | 0.0107 | 0.0009 | 0.0004 | 0.0036 | 0.0022 | 0.0023 | 0.0129 |
Bud Periphery | 0.0021 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0013 | 0.0004 | 0.0008 | 0.0041 | 0.0004 | 0.0007 | 0.0005 | 0.0001 | 0.0042 | 0 | 0.0011 | 0.0017 |
Bud Site | 0.025 | 0.0111 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0035 | 0.0017 | 0.0006 | 0.0023 | 0.0014 | 0.0004 | 0.0012 | 0.0012 | 0.0089 | 0 | 0.0201 | 0.0001 |
Cell Periphery | 0.0019 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.001 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.034 | 0.041 | 0.0646 | 0.0673 | 0.0895 | 0.0544 | 0.0395 | 0.0448 | 0.0433 | 0.0385 | 0.0424 | 0.0439 | 0.0356 | 0.0833 | 0.0688 | 0.0535 | 0.0169 | 0.0968 |
Cytoplasmic Foci | 0.099 | 0.0344 | 0.0504 | 0.0454 | 0.025 | 0.0512 | 0.0615 | 0.0498 | 0.0533 | 0.0278 | 0.0622 | 0.0438 | 0.0819 | 0.0606 | 0.0578 | 0.0611 | 0.0086 | 0.0308 |
Eisosomes | 0.0019 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0011 | 0.0004 | 0.0006 | 0.0003 | 0.0005 | 0.0003 |
Endoplasmic Reticulum | 0.0152 | 0.049 | 0.036 | 0.0102 | 0.0136 | 0.0251 | 0.0171 | 0.0225 | 0.0137 | 0.0093 | 0.0186 | 0.0118 | 0.0244 | 0.0246 | 0.0241 | 0.0067 | 0.074 | 0.0237 |
Endosome | 0.0273 | 0.0384 | 0.0395 | 0.0081 | 0.0745 | 0.0367 | 0.0493 | 0.0493 | 0.0546 | 0.0393 | 0.0527 | 0.0405 | 0.0671 | 0.0476 | 0.034 | 0.009 | 0.006 | 0.0248 |
Golgi | 0.0159 | 0.0048 | 0.0051 | 0.0052 | 0.0051 | 0.012 | 0.0085 | 0.0036 | 0.0053 | 0.006 | 0.0184 | 0.005 | 0.013 | 0.0039 | 0.006 | 0.0021 | 0.0054 | 0.0031 |
Lipid Particles | 0.2368 | 0.1686 | 0.1333 | 0.1363 | 0.0621 | 0.1401 | 0.1713 | 0.1153 | 0.1104 | 0.0973 | 0.1578 | 0.1336 | 0.1874 | 0.1139 | 0.1108 | 0.0656 | 0.069 | 0.0806 |
Mitochondria | 0.0104 | 0.0014 | 0.0195 | 0.0016 | 0.0021 | 0.0327 | 0.0145 | 0.0023 | 0.0052 | 0.0122 | 0.0075 | 0.008 | 0.0063 | 0.0014 | 0.0047 | 0.0005 | 0.001 | 0.003 |
None | 0.2388 | 0.3781 | 0.4405 | 0.658 | 0.5889 | 0.2941 | 0.3629 | 0.4306 | 0.4123 | 0.5877 | 0.3681 | 0.432 | 0.3726 | 0.4907 | 0.5465 | 0.7 | 0.6616 | 0.5679 |
Nuclear Periphery | 0.124 | 0.2137 | 0.1368 | 0.0357 | 0.1085 | 0.2778 | 0.1727 | 0.1516 | 0.2031 | 0.1157 | 0.1296 | 0.1786 | 0.1302 | 0.1055 | 0.0496 | 0.0509 | 0.0799 | 0.0971 |
Nucleolus | 0.0025 | 0.0017 | 0.0014 | 0.0006 | 0.0009 | 0.0005 | 0.0022 | 0.0049 | 0.0021 | 0.0016 | 0.0035 | 0.0022 | 0.0007 | 0.0022 | 0.0015 | 0.0005 | 0.0002 | 0.0011 |
Nucleus | 0.0072 | 0.0043 | 0.0081 | 0.0022 | 0.0048 | 0.0038 | 0.006 | 0.0204 | 0.0071 | 0.0051 | 0.0085 | 0.0093 | 0.0021 | 0.0091 | 0.0033 | 0.0014 | 0.0006 | 0.013 |
Peroxisomes | 0.0175 | 0.0029 | 0.0073 | 0.0059 | 0.0017 | 0.004 | 0.0236 | 0.007 | 0.0102 | 0.0108 | 0.0366 | 0.0128 | 0.0221 | 0.0025 | 0.0224 | 0.0182 | 0.0036 | 0.0053 |
Punctate Nuclear | 0.0532 | 0.0115 | 0.0231 | 0.0176 | 0.0112 | 0.0151 | 0.0381 | 0.082 | 0.06 | 0.0207 | 0.0574 | 0.0456 | 0.0097 | 0.0387 | 0.0243 | 0.0249 | 0.0019 | 0.0295 |
Vacuole | 0.0051 | 0.0073 | 0.0056 | 0.0019 | 0.0041 | 0.0027 | 0.0061 | 0.0064 | 0.0038 | 0.0041 | 0.0042 | 0.0039 | 0.0056 | 0.0062 | 0.0042 | 0.0015 | 0.001 | 0.0025 |
Vacuole Periphery | 0.012 | 0.0124 | 0.0087 | 0.0025 | 0.0056 | 0.0091 | 0.0068 | 0.0059 | 0.0073 | 0.0062 | 0.0054 | 0.0064 | 0.0062 | 0.0057 | 0.0027 | 0.0009 | 0.0024 | 0.0035 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0739 | 11.5817 | 21.9628 | 23.6027 | 20.064 | 17.2649 | 25.3722 | 32.0536 | 27.7334 | 25.2559 |
Translational Efficiency | 0.7289 | 1.2173 | 0.8381 | 0.7152 | 0.5949 | 0.7467 | 0.6384 | 0.6263 | 0.6666 | 0.6183 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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