Standard name
Human Ortholog
Description Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.07 0.07 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.08 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.97 0.99 0.95 0.83 0.75 0.7 0.6 0.5 0.49 0.98 0.99 0.98 0.94 0.98 0.94 0.96 0.92 0.88 0.93 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.12 0.12 0.13 0.13 0.12 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.06 0 0.12 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.11 0.14 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0.08 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 1 0 2 15 16 20 30 29 32 0 0 0 0 0 0 0 0 1 0 1 1
Bud 1 0 3 1 1 1 1 3 4 16 7 10 1 2 0 0 0 0 0 0 2 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 0 5 9 8 5 16 18 9 12 10 9 8 18 15 19 10 12 0 0 0 0 0 0
Cytoplasm 238 35 223 220 301 326 425 354 211 241 141 156 221 475 537 183 222 146 238 60 210 151 367 460
Endoplasmic Reticulum 3 0 2 2 2 11 59 57 40 52 35 31 2 1 4 7 7 5 1 0 7 1 3 14
Endosome 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 1 1 1 1
Golgi 1 0 1 0 0 3 3 20 18 14 35 24 0 1 1 0 1 1 0 0 3 1 1 1
Mitochondria 2 1 1 4 0 3 1 6 31 45 39 51 0 2 0 4 0 4 0 0 3 0 0 1
Nucleus 2 0 1 2 0 2 1 0 0 3 3 2 0 2 3 1 0 0 1 0 0 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1 3 2
Nucleolus 0 1 1 0 0 0 1 4 1 9 9 7 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 1 0 1 0 3 11 31 8 32 26 40 0 0 2 4 3 4 2 2 3 2 3 6
Unique Cell Count 246 36 230 226 304 343 509 475 303 400 282 321 225 482 548 195 227 155 249 66 240 164 390 500
Labelled Cell Count 261 38 240 240 312 356 533 509 342 456 334 363 233 501 562 220 245 172 249 66 240 164 390 500


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 17.4 18.0 19.3 26.8 26.9 28.3 26.8 25.6 21.6 23.3 22.8 23.0 22.1 22.1 24.8 31.5 34.0 19.7 19.1 20.3
Std Deviation (1e-4) 3.5 3.2 3.3 3.8 5.7 6.0 6.6 7.0 7.5 6.5 7.4 6.9 4.5 4.7 4.3 8.6 10.5 12.6 3.5 3.4 3.9
Intensity Change (Log2) – – – 0.1 0.58 0.58 0.65 0.57 0.51 0.27 0.37 0.34 0.36 0.3 0.29 0.46 0.81 0.92 0.13 0.09 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.3 1.6 3.0 3.4 4.2 4.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.9 0 1.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 0.3 0 0.7 1.1 0.6 0.6 0.9 0.5 0.9 1.1 0.5 3.4 1.3 2.6
Cytoplasm 0.2 1.7 -1.1 -5.2 -7.2 -8.0 -10.1 -11.7 -12.1 0.9 1.4 0.9 -1.5 0.6 -1.3
Endoplasmic Reticulum 0 0 1.8 4.9 5.0 5.2 5.2 5.0 4.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2.8 3.4 2.4 5.3 3.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.7 5.0 5.6 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.0 0 4.4 4.7 5.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.7261 15.4194 14.8732 15.3353 14.2264 14.4342 12.4564 16.3832 12.8628 11.2614 10.3281 13.4449 13.4572 16.3437 15.0317 14.8338 13.3916 14.88
Actin 0.0281 0.0022 0.0078 0.0055 0.0056 0.003 0.0726 0.0019 0.0088 0.0049 0.0165 0.0144 0.037 0.0065 0.0317 0.025 0.0358 0.007
Bud 0.0028 0.0016 0.0018 0.0035 0.0018 0.0012 0.0026 0.0005 0.0017 0.0038 0.0104 0.0008 0.0034 0.0016 0.0051 0.0067 0.003 0.0027
Bud Neck 0.0047 0.0006 0.0019 0.0015 0.0019 0.0044 0.0014 0.0003 0.0006 0.0019 0.0012 0.0023 0.0025 0.0006 0.0023 0.0014 0.0025 0.0045
Bud Periphery 0.0021 0.0007 0.0012 0.0029 0.0005 0.0008 0.0017 0.0001 0.0005 0.0016 0.0026 0.0003 0.0018 0.0005 0.0019 0.0042 0.0013 0.0018
Bud Site 0.0142 0.002 0.0013 0.0005 0.0008 0.0003 0.003 0.0005 0.0007 0.0045 0.0009 0.0002 0.0077 0.0032 0.0105 0.0035 0.0012 0.0005
Cell Periphery 0.0008 0.0003 0.0003 0.0001 0.0001 0.0002 0.0004 0 0.0001 0.0003 0.0001 0 0.0006 0.0003 0.0003 0.0004 0.0007 0.0003
Cytoplasm 0.7125 0.9285 0.8865 0.7717 0.8844 0.8707 0.7111 0.989 0.9171 0.8369 0.7961 0.9127 0.7423 0.9439 0.8358 0.8713 0.7612 0.8559
Cytoplasmic Foci 0.0169 0.0151 0.0157 0.0311 0.0165 0.0215 0.0359 0.0035 0.009 0.0221 0.0393 0.0245 0.0398 0.0149 0.0288 0.0285 0.0728 0.0304
Eisosomes 0.0003 0 0 0 0 0 0.0002 0 0 0 0 0 0.0003 0 0.0005 0.0001 0.0002 0
Endoplasmic Reticulum 0.0342 0.0039 0.0158 0.005 0.0058 0.0159 0.0071 0.0004 0.0021 0.0015 0.0006 0.0077 0.0252 0.002 0.0026 0.0037 0.0057 0.0061
Endosome 0.0542 0.016 0.0261 0.0793 0.0308 0.039 0.0486 0.0008 0.0322 0.0481 0.0894 0.0237 0.0494 0.0097 0.0191 0.0138 0.0422 0.0377
Golgi 0.008 0.0047 0.01 0.0385 0.0138 0.0036 0.0273 0.0003 0.0135 0.0271 0.0299 0.0027 0.0155 0.0073 0.0125 0.0053 0.0135 0.0196
Lipid Particles 0.0043 0.0002 0.0003 0.0108 0.0078 0.0002 0.0215 0 0.0017 0.0042 0.0015 0.0001 0.0067 0 0.0074 0.0012 0.0163 0.0022
Mitochondria 0.0244 0.0153 0.0114 0.0285 0.0052 0.0114 0.0206 0.0006 0.0027 0.0289 0.0043 0.0027 0.0097 0.0033 0.0184 0.0054 0.0128 0.02
None 0.0048 0.0005 0.0009 0.0006 0.0006 0.0009 0.0036 0.0003 0.0004 0.0005 0.0004 0.0006 0.01 0.0005 0.002 0.0203 0.001 0.0009
Nuclear Periphery 0.0309 0.002 0.0055 0.002 0.0029 0.0063 0.0044 0.0002 0.0007 0.001 0.0004 0.0015 0.0104 0.0009 0.001 0.0009 0.0006 0.0011
Nucleolus 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0002 0 0 0.0028 0 0.0002 0.0001 0.0001 0.0001
Nucleus 0.015 0.0029 0.0044 0.0028 0.0051 0.0063 0.0031 0.0009 0.0018 0.0017 0.0013 0.0025 0.0106 0.0018 0.0025 0.0018 0.0014 0.0025
Peroxisomes 0.0032 0.0015 0.0012 0.0042 0.0041 0.0006 0.019 0.0003 0.0029 0.0037 0.0025 0.0011 0.0047 0.0012 0.0122 0.0022 0.025 0.0021
Punctate Nuclear 0.0021 0.0002 0.0004 0.0002 0.0003 0.0005 0.0032 0.0001 0.0002 0.0003 0.0002 0.0003 0.0107 0.0001 0.0011 0.0005 0.001 0.0003
Vacuole 0.0277 0.0016 0.0052 0.0064 0.0083 0.0087 0.0088 0.0002 0.0022 0.0045 0.0015 0.0012 0.0068 0.0013 0.0032 0.0026 0.0009 0.0029
Vacuole Periphery 0.0083 0.0004 0.0021 0.0046 0.0035 0.0043 0.0036 0.0001 0.001 0.002 0.0009 0.0007 0.0021 0.0004 0.0009 0.001 0.0007 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 251.4328 258.5315 234.3605 233.1913 227.5524 198.2893 221.0528 232.9874 255.0202 227.6315
Translational Efficiency 1.288 1.2527 1.4142 1.6485 1.3525 1.5044 1.5642 1.3444 1.4456 1.3986

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Dug1

Dug1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dug1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available