Standard name | |
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Human Ortholog | |
Description | Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 3 | 4 | 4 | 2 | 5 | 1 | 2 | 1 | – | 2 | 1 | 0 | 2 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 0 | 1 | 5 | 7 | 9 | 9 | 4 | 5 | 6 | 3 | 2 | 1 | 3 | – | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 237 | 391 | 142 | 144 | 229 | 183 | 307 | 320 | 191 | 223 | 146 | 154 | 262 | 413 | 463 | – | 242 | 247 | 233 | 386 | 152 | 280 | 275 | 263 |
Endoplasmic Reticulum | 1 | 1 | 0 | 2 | 9 | 5 | 6 | 4 | 5 | 6 | 2 | 4 | 2 | 1 | 1 | – | 10 | 14 | 0 | 1 | 0 | 1 | 3 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | – | 4 | 0 | 0 | 0 | 1 | 1 | 1 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 1 | 3 | 0 | 2 | 0 | 4 | 17 | 20 | 76 | 54 | 56 | 52 | 0 | 0 | 0 | – | 11 | 12 | 1 | 2 | 1 | 0 | 1 | 3 |
Nucleus | 2 | 3 | 0 | 1 | 0 | 1 | 8 | 10 | 3 | 6 | 3 | 10 | 0 | 1 | 1 | – | 3 | 1 | 0 | 1 | 0 | 0 | 3 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 2 | 9 | 4 | 4 | 41 | 63 | 49 | 38 | 14 | 21 | 6 | 16 | 20 | – | 11 | 15 | 1 | 7 | 0 | 1 | 4 | 5 |
Unique Cell Count | 241 | 397 | 143 | 153 | 240 | 198 | 358 | 406 | 283 | 306 | 202 | 226 | 269 | 426 | 475 | 261 | 262 | 239 | 405 | 160 | 291 | 300 | 296 | |
Labelled Cell Count | 247 | 409 | 144 | 159 | 248 | 206 | 390 | 430 | 333 | 338 | 232 | 253 | 274 | 434 | 489 | 289 | 294 | 239 | 405 | 160 | 291 | 300 | 296 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.2 | 8.3 | 11.0 | 10.6 | 10.6 | 8.6 | 8.2 | 8.4 | 6.8 | 6.7 | 6.4 | 6.7 | 11.4 | 11.6 | 11.6 | 4.0 | 4.9 | 5.3 | 10.8 | 10.7 | 11.2 |
Std Deviation (1e-4) | 2.5 | 1.8 | 2.0 | 2.4 | 2.1 | 1.7 | 1.8 | 1.8 | 1.7 | 1.5 | 1.5 | 1.4 | 2.9 | 2.8 | 2.7 | 1.2 | 1.6 | 2.0 | 2.3 | 2.4 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.05 | -0.34 | -0.42 | -0.39 | -0.69 | -0.7 | -0.78 | -0.72 | 0.06 | 0.08 | 0.08 | -1.45 | -1.15 | -1.04 | -0.02 | -0.04 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | -2.5 | -2.1 | -3.0 | -4.5 | -5.8 | -7.5 | -6.7 | -6.7 | -7.3 | -1.3 | -1.6 | -1.3 | – | -2.9 | -2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.7 | 6.8 | 5.4 | 6.9 | 6.2 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 2.0 | 0 | 0 | 3.6 | 4.5 | 4.8 | 3.8 | 2.4 | 3.1 | 0 | 0 | 1.6 | – | 0 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.468 | 10.9743 | 10.404 | 11.0385 | 9.434 | 10.6973 | 6.9168 | 10.0047 | 8.5114 | 9.3688 | 8.4528 | 9.4625 | 5.305 | 6.9971 | 6.1844 | 6.7878 | 5.459 | 6.1459 |
Actin | 0.0204 | 0.0011 | 0.0223 | 0.0009 | 0.01 | 0.0032 | 0.0235 | 0.0009 | 0.0109 | 0.0168 | 0.0042 | 0.0103 | 0.0147 | 0.0002 | 0.0062 | 0.0004 | 0.0006 | 0.0002 |
Bud | 0.0011 | 0.0073 | 0.0006 | 0.0008 | 0.0017 | 0.0005 | 0.0018 | 0.0003 | 0.0009 | 0.0014 | 0.0022 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0016 | 0.0001 |
Bud Neck | 0.0026 | 0.0004 | 0.0024 | 0.0006 | 0.001 | 0.002 | 0.0024 | 0.0002 | 0.0004 | 0.001 | 0.0005 | 0.0009 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0009 |
Bud Periphery | 0.0009 | 0.0008 | 0.0005 | 0.0004 | 0.0029 | 0.0002 | 0.0022 | 0.0001 | 0.0009 | 0.0005 | 0.0034 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0.0005 | 0 |
Bud Site | 0.0046 | 0.0088 | 0.0061 | 0.0002 | 0.0008 | 0.0003 | 0.0019 | 0.0005 | 0.001 | 0.0006 | 0.0025 | 0.0002 | 0.0011 | 0.0003 | 0.0006 | 0.0001 | 0.0006 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8236 | 0.9516 | 0.9044 | 0.9701 | 0.8132 | 0.9254 | 0.8087 | 0.8802 | 0.8804 | 0.8623 | 0.8523 | 0.94 | 0.8916 | 0.9865 | 0.9526 | 0.9757 | 0.8964 | 0.9651 |
Cytoplasmic Foci | 0.0217 | 0.0077 | 0.0064 | 0.0035 | 0.0585 | 0.0074 | 0.0133 | 0.0062 | 0.0118 | 0.0309 | 0.0131 | 0.0057 | 0.018 | 0.002 | 0.0096 | 0.0029 | 0.0315 | 0.0033 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0011 | 0.0029 | 0.0018 | 0.0069 | 0.0036 | 0.0046 | 0.0031 | 0.0069 | 0.001 | 0.0118 | 0.0033 | 0.0044 | 0.0007 | 0.0035 | 0.0015 | 0.0009 | 0.0015 |
Endosome | 0.0252 | 0.0017 | 0.0031 | 0.0016 | 0.0233 | 0.0084 | 0.0158 | 0.0176 | 0.0142 | 0.0144 | 0.0326 | 0.005 | 0.0125 | 0.0008 | 0.0025 | 0.0012 | 0.0084 | 0.0017 |
Golgi | 0.0077 | 0.0003 | 0.0022 | 0.0002 | 0.0061 | 0.0014 | 0.0053 | 0.0016 | 0.0102 | 0.0299 | 0.0099 | 0.0006 | 0.0025 | 0.0001 | 0.0006 | 0.0001 | 0.0017 | 0.0001 |
Lipid Particles | 0.0029 | 0 | 0.0043 | 0 | 0.0091 | 0.0011 | 0.0069 | 0.0017 | 0.0024 | 0.0036 | 0.0067 | 0.0002 | 0.0025 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Mitochondria | 0.0054 | 0.0003 | 0.0036 | 0.0002 | 0.0024 | 0.0062 | 0.0109 | 0.0003 | 0.003 | 0.0031 | 0.0204 | 0.0011 | 0.0018 | 0.0001 | 0.0003 | 0.0001 | 0.0035 | 0.0001 |
None | 0.0407 | 0.0038 | 0.0116 | 0.0041 | 0.0147 | 0.0105 | 0.0513 | 0.0672 | 0.0211 | 0.0042 | 0.0287 | 0.0182 | 0.0174 | 0.003 | 0.0068 | 0.0021 | 0.0349 | 0.0035 |
Nuclear Periphery | 0.0058 | 0.0014 | 0.0016 | 0.0022 | 0.0032 | 0.0059 | 0.0147 | 0.0035 | 0.0104 | 0.0014 | 0.0019 | 0.0015 | 0.0035 | 0.0005 | 0.0019 | 0.0021 | 0.0012 | 0.0017 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0165 | 0.0087 | 0.0177 | 0.0109 | 0.006 | 0.0196 | 0.0094 | 0.0135 | 0.006 | 0.0101 | 0.0031 | 0.0109 | 0.01 | 0.0048 | 0.01 | 0.0123 | 0.0059 | 0.0207 |
Peroxisomes | 0.0064 | 0.0002 | 0.0075 | 0.0001 | 0.0169 | 0.0007 | 0.0186 | 0.0003 | 0.0112 | 0.0171 | 0.0006 | 0.0002 | 0.0074 | 0 | 0.002 | 0 | 0.0088 | 0 |
Punctate Nuclear | 0.003 | 0.0037 | 0.0009 | 0.0009 | 0.0202 | 0.0018 | 0.0025 | 0.0019 | 0.0065 | 0.0008 | 0.0009 | 0.0008 | 0.0098 | 0.0002 | 0.0006 | 0.0006 | 0.0007 | 0.0003 |
Vacuole | 0.003 | 0.0007 | 0.0008 | 0.001 | 0.0021 | 0.0011 | 0.0031 | 0.0005 | 0.001 | 0.0004 | 0.0034 | 0.0006 | 0.0012 | 0.0003 | 0.0007 | 0.0003 | 0.0014 | 0.0006 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0022 | 0.0002 | 0.0004 | 0.0002 | 0.0015 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.6856 | 15.551 | 14.8365 | 21.3779 | 20.1381 | 39.7403 | 25.8316 | 23.4988 | 35.6115 | 25.8961 |
Translational Efficiency | 0.9008 | 1.7237 | 1.3335 | 1.2418 | 1.3254 | 0.9323 | 1.4442 | 1.4371 | 0.9815 | 1.1185 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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