Standard name | |
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Human Ortholog | |
Description | Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 5 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 0 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 8 | 4 | 0 | 1 | 9 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 1 | 4 | 3 | 4 | 5 | 4 | 2 | 3 | 6 | 2 | 6 | 10 | 6 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 244 | 63 | 83 | 88 | 85 | 104 | 132 | 112 | 61 | 89 | 45 | 82 | 171 | 303 | 315 | 109 | 112 | 99 | 226 | 81 | 77 | 110 | 205 | 114 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 1 | 1 | 11 | 13 | 3 | 8 | 3 | 5 | 1 | 0 | 1 | 7 | 4 | 6 | 1 | 0 | 3 | 0 | 2 | 3 |
Endosome | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 1 | 2 | 2 | 3 | 4 | 6 | 2 | 10 | 16 | 2 | 11 | 1 | 2 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 2 | 0 | 1 | 4 | 2 | 12 | 15 | 7 | 12 | 4 | 2 | 0 | 5 | 3 | 2 | 5 | 1 | 1 | 0 | 0 | 1 | 2 | 1 |
Unique Cell Count | 247 | 67 | 83 | 91 | 92 | 114 | 161 | 148 | 82 | 124 | 54 | 106 | 175 | 313 | 323 | 112 | 122 | 103 | 250 | 92 | 86 | 118 | 227 | 148 |
Labelled Cell Count | 255 | 70 | 86 | 95 | 97 | 117 | 170 | 155 | 87 | 141 | 58 | 116 | 181 | 325 | 340 | 127 | 133 | 118 | 250 | 92 | 86 | 118 | 227 | 148 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 21.9 | 18.9 | 15.1 | 22.4 | 25.9 | 45.1 | 45.4 | 50.4 | 52.1 | 60.4 | 53.8 | 12.5 | 23.6 | 44.9 | 13.8 | 18.2 | 21.2 | 16.3 | 13.4 | 13.7 |
Std Deviation (1e-4) | 8.1 | 15.3 | 11.5 | 10.1 | 20.0 | 19.5 | 30.7 | 30.3 | 39.1 | 42.3 | 44.0 | 40.2 | 12.7 | 23.3 | 33.6 | 15.1 | 31.7 | 25.4 | 37.1 | 10.7 | 12.4 |
Intensity Change (Log2) | – | – | – | -0.33 | 0.24 | 0.45 | 1.25 | 1.26 | 1.41 | 1.46 | 1.67 | 1.51 | -0.59 | 0.32 | 1.24 | -0.46 | -0.06 | 0.16 | -0.22 | -0.5 | -0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -2.6 | -2.8 | -4.1 | -4.9 | -4.9 | -5.3 | -3.8 | -4.6 | -1.4 | -1.6 | -1.4 | -1.5 | -2.7 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.6 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 35.6444 | 47.0392 | 40.9041 | 45.4819 | 46.5966 | 49.6432 | 27.744 | 33.8023 | 35.3307 | 23.8904 | 16.7745 | 30.0711 | 30.6636 | 43.778 | 40.9372 | 50.4078 | 39.8668 | 44.7291 |
Actin | 0.0038 | 0.0026 | 0.004 | 0.0002 | 0.0328 | 0.0041 | 0.0134 | 0.0024 | 0.0029 | 0.0056 | 0.0071 | 0.0004 | 0.0272 | 0.0028 | 0.0163 | 0.0485 | 0.0382 | 0.0026 |
Bud | 0.0026 | 0.0115 | 0.0023 | 0.0004 | 0.0008 | 0.0015 | 0.0011 | 0.0039 | 0.0022 | 0.0286 | 0.0126 | 0.0006 | 0.0064 | 0.0112 | 0.0053 | 0.0014 | 0.0006 | 0.0011 |
Bud Neck | 0.001 | 0.0004 | 0.0006 | 0.0006 | 0.0009 | 0.0025 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0003 | 0.0009 | 0.0005 | 0.0013 | 0.0022 |
Bud Periphery | 0.0011 | 0.0054 | 0.0009 | 0.0001 | 0.0007 | 0.0012 | 0.0007 | 0.0006 | 0.0011 | 0.0291 | 0.0114 | 0.0001 | 0.0037 | 0.0033 | 0.0031 | 0.0007 | 0.0003 | 0.0005 |
Bud Site | 0.002 | 0.0101 | 0.0013 | 0.0002 | 0.0013 | 0.0004 | 0.0018 | 0.0065 | 0.0006 | 0.0054 | 0.0008 | 0 | 0.0042 | 0.006 | 0.0041 | 0.0011 | 0.0007 | 0.0002 |
Cell Periphery | 0.0012 | 0.0012 | 0.0004 | 0.0001 | 0.0002 | 0.001 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0009 | 0.0004 | 0.0013 | 0.0014 | 0.001 | 0.0002 |
Cytoplasm | 0.8655 | 0.954 | 0.9081 | 0.9346 | 0.9258 | 0.9621 | 0.8984 | 0.9665 | 0.968 | 0.8625 | 0.8818 | 0.989 | 0.8134 | 0.9662 | 0.9151 | 0.8716 | 0.885 | 0.9277 |
Cytoplasmic Foci | 0.0251 | 0.0019 | 0.0103 | 0.007 | 0.0017 | 0.0019 | 0.0121 | 0.0043 | 0.0047 | 0.0184 | 0.005 | 0.0021 | 0.02 | 0.0027 | 0.0068 | 0.0238 | 0.0088 | 0.0043 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0002 | 0.0005 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0012 | 0.0067 | 0.0009 | 0.0056 | 0.0173 | 0.0158 | 0.0018 | 0.0016 | 0.0011 | 0.0044 | 0.003 | 0.013 | 0.0006 | 0.0029 | 0.0017 | 0.0033 | 0.0026 |
Endosome | 0.0062 | 0.0007 | 0.0054 | 0.0017 | 0.0074 | 0.0018 | 0.0247 | 0.0044 | 0.0025 | 0.0221 | 0.0187 | 0.001 | 0.0152 | 0.0003 | 0.0045 | 0.002 | 0.0069 | 0.0095 |
Golgi | 0.0034 | 0.0001 | 0.0021 | 0.001 | 0.0028 | 0.0002 | 0.0084 | 0.004 | 0.0002 | 0.0066 | 0.0029 | 0.0001 | 0.0047 | 0.0001 | 0.0043 | 0.0022 | 0.0039 | 0.0207 |
Lipid Particles | 0.007 | 0.0001 | 0.0024 | 0.0154 | 0.002 | 0.0001 | 0.0038 | 0.0002 | 0 | 0.0054 | 0.0001 | 0 | 0.0086 | 0 | 0.002 | 0.0052 | 0.0045 | 0.0013 |
Mitochondria | 0.0059 | 0.0003 | 0.0212 | 0.0012 | 0.001 | 0.0001 | 0.0017 | 0.001 | 0.0002 | 0.0016 | 0.0375 | 0.0001 | 0.0033 | 0.0003 | 0.0071 | 0.0064 | 0.0056 | 0.0007 |
None | 0.01 | 0.0009 | 0.0054 | 0.0004 | 0.0008 | 0.0004 | 0.0075 | 0.0017 | 0.0011 | 0.0018 | 0.0017 | 0.0015 | 0.0239 | 0.0022 | 0.0042 | 0.0128 | 0.0152 | 0.0021 |
Nuclear Periphery | 0.0087 | 0.0014 | 0.0062 | 0.0012 | 0.0048 | 0.0014 | 0.0029 | 0.0004 | 0.0046 | 0.0012 | 0.0032 | 0.0003 | 0.0132 | 0.0008 | 0.0018 | 0.0029 | 0.0038 | 0.0049 |
Nucleolus | 0.0032 | 0.0001 | 0.0014 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0014 | 0 | 0.0008 | 0.0034 | 0.0027 | 0.0003 |
Nucleus | 0.0079 | 0.0017 | 0.0039 | 0.0015 | 0.0018 | 0.002 | 0.0018 | 0.0008 | 0.007 | 0.0012 | 0.0035 | 0.0007 | 0.011 | 0.0013 | 0.0029 | 0.0022 | 0.0032 | 0.0049 |
Peroxisomes | 0.0036 | 0.0001 | 0.0015 | 0.028 | 0.0003 | 0 | 0.001 | 0 | 0.0001 | 0.0029 | 0.0004 | 0 | 0.0101 | 0.0001 | 0.0007 | 0.0015 | 0.0013 | 0.0002 |
Punctate Nuclear | 0.0109 | 0.0001 | 0.0024 | 0.0002 | 0.0009 | 0.0001 | 0.0009 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0067 | 0.0003 | 0.001 | 0.0011 | 0.0018 | 0.0008 |
Vacuole | 0.0158 | 0.0049 | 0.0097 | 0.0044 | 0.0063 | 0.0017 | 0.0023 | 0.0006 | 0.0021 | 0.004 | 0.0044 | 0.0005 | 0.0092 | 0.0011 | 0.0115 | 0.0076 | 0.008 | 0.0081 |
Vacuole Periphery | 0.0039 | 0.0014 | 0.0037 | 0.0008 | 0.0017 | 0.0004 | 0.0006 | 0.0001 | 0.0005 | 0.0011 | 0.0039 | 0.0001 | 0.0024 | 0.0001 | 0.0034 | 0.0016 | 0.0035 | 0.0052 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 109.735 | 37.209 | 53.7153 | 170.9298 | 74.0818 | 330.5355 | 109.1037 | 135.9642 | 281.4788 | 140.7014 |
Translational Efficiency | 1.7184 | 1.3958 | 1.3504 | 1.8136 | 1.6242 | 1.6904 | 1.6523 | 1.5951 | 1.6985 | 1.7277 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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