Standard name | |
---|---|
Human Ortholog | |
Description | Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud | 0 | 2 | 0 | 0 | 2 | 1 | 3 | 3 | 3 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 2 | 2 | 4 | 4 | 2 | 1 | 3 | 2 | 0 | 3 | 9 | 5 | 6 | 9 | 21 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 236 | 147 | 180 | 129 | 176 | 255 | 276 | 282 | 140 | 252 | 123 | 131 | 454 | 639 | 688 | 305 | 420 | 379 | 252 | 147 | 181 | 346 | 595 | 552 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 0 | 4 | 1 | 16 | 10 | 6 | 1 | 0 | 1 | 0 | 1 | 3 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 7 | 3 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 10 | 2 | 1 | 7 | 9 | 56 | 93 | 89 | 101 | 159 | 103 | 97 | 0 | 0 | 0 | 3 | 1 | 4 | 3 | 1 | 0 | 0 | 7 | 5 |
Nucleus | 0 | 2 | 0 | 1 | 0 | 1 | 9 | 5 | 3 | 8 | 5 | 10 | 0 | 3 | 3 | 3 | 5 | 5 | 0 | 0 | 0 | 4 | 1 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 12 | 2 | 0 | 2 | 2 | 3 | 20 | 41 | 36 | 36 | 27 | 17 | 2 | 0 | 6 | 13 | 22 | 14 | 3 | 2 | 0 | 5 | 5 | 7 |
Unique Cell Count | 249 | 151 | 180 | 135 | 182 | 278 | 348 | 374 | 229 | 376 | 201 | 220 | 457 | 644 | 692 | 314 | 437 | 396 | 270 | 158 | 184 | 363 | 638 | 591 |
Labelled Cell Count | 263 | 157 | 184 | 144 | 193 | 324 | 407 | 425 | 288 | 463 | 263 | 269 | 466 | 652 | 705 | 349 | 481 | 435 | 270 | 158 | 184 | 363 | 638 | 591 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.5 | 6.5 | 6.2 | 6.3 | 5.3 | 5.2 | 5.4 | 5.4 | 5.1 | 4.9 | 5.2 | 7.9 | 8.1 | 8.2 | 9.2 | 9.0 | 9.4 | 7.5 | 7.8 | 8.0 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.3 | 1.8 | 1.9 | 1.1 | 1.1 | 1.3 | 1.5 | 1.0 | 1.5 | 1.3 | 1.1 | 1.2 | 1.2 | 1.7 | 1.7 | 1.7 | 1.3 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.06 | -0.29 | -0.32 | -0.27 | -0.28 | -0.35 | -0.42 | -0.32 | 0.27 | 0.3 | 0.32 | 0.49 | 0.47 | 0.52 | 0.2 | 0.26 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 2.5 |
Cytoplasm | -2.9 | -2.5 | -4.0 | -6.6 | -7.3 | -9.5 | -8.7 | -9.4 | -9.7 | -1.1 | -1.2 | -1.0 | -2.3 | -2.7 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.2 | 7.5 | 6.9 | 10.1 | 10.2 | 0 | 10.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.3 | 4.6 | 5.6 | 4.3 | 5.1 | 3.8 | 0 | 0 | 0 | 0 | 3.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5554 | 3.4903 | 3.1044 | 2.5478 | 2.3347 | 2.9124 | 1.2311 | 2.2861 | 1.882 | 1.2655 | 1.1453 | 1.7179 | 1.7544 | 2.5714 | 2.1807 | 1.8384 | 1.5425 | 1.7215 |
Actin | 0.0253 | 0.0004 | 0.0202 | 0.0039 | 0.0282 | 0.0134 | 0.0573 | 0.0089 | 0.0108 | 0.0381 | 0.0024 | 0.0072 | 0.0188 | 0.0016 | 0.0064 | 0.0024 | 0.0137 | 0.0054 |
Bud | 0.0004 | 0.0003 | 0.0022 | 0.0004 | 0.0003 | 0.0005 | 0.0013 | 0.0023 | 0.0002 | 0.0026 | 0.0002 | 0.003 | 0.0125 | 0.0113 | 0.0138 | 0.0043 | 0.0013 | 0.0032 |
Bud Neck | 0.0013 | 0.0003 | 0.0044 | 0.0006 | 0.0006 | 0.0018 | 0.0033 | 0.0003 | 0.0003 | 0.0034 | 0.0012 | 0.0014 | 0.0009 | 0.0003 | 0.0007 | 0.0016 | 0.0022 | 0.0028 |
Bud Periphery | 0.0004 | 0.0001 | 0.0022 | 0.0005 | 0.0002 | 0.0004 | 0.0018 | 0.002 | 0.0001 | 0.0056 | 0.0001 | 0.0017 | 0.0074 | 0.0032 | 0.0047 | 0.0019 | 0.0011 | 0.0013 |
Bud Site | 0.0026 | 0.0021 | 0.0127 | 0.0005 | 0.0002 | 0.0003 | 0.005 | 0.0054 | 0.0005 | 0.0198 | 0.0003 | 0.0003 | 0.0147 | 0.0108 | 0.0082 | 0.001 | 0.0011 | 0.0005 |
Cell Periphery | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.7483 | 0.8351 | 0.7958 | 0.7696 | 0.8589 | 0.8377 | 0.6731 | 0.8399 | 0.8544 | 0.8034 | 0.8604 | 0.8452 | 0.6977 | 0.8403 | 0.801 | 0.7611 | 0.7634 | 0.8446 |
Cytoplasmic Foci | 0.0145 | 0.0017 | 0.0118 | 0.0124 | 0.0093 | 0.0127 | 0.0288 | 0.0057 | 0.0065 | 0.0142 | 0.0143 | 0.0091 | 0.0264 | 0.0168 | 0.0141 | 0.0487 | 0.0186 | 0.0178 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0088 | 0.0017 | 0.0031 | 0.0102 | 0.0029 | 0.0044 | 0.0074 | 0.0032 | 0.0032 | 0.0035 | 0.006 | 0.0033 | 0.0085 | 0.002 | 0.0027 | 0.0026 | 0.0035 | 0.0025 |
Endosome | 0.0206 | 0.0006 | 0.0084 | 0.0205 | 0.0053 | 0.0089 | 0.0386 | 0.004 | 0.008 | 0.0151 | 0.0104 | 0.01 | 0.0264 | 0.0016 | 0.0139 | 0.0354 | 0.0146 | 0.014 |
Golgi | 0.0057 | 0.0001 | 0.0048 | 0.0023 | 0.0012 | 0.0037 | 0.0136 | 0.0024 | 0.0024 | 0.0069 | 0.0018 | 0.0048 | 0.003 | 0.0002 | 0.0043 | 0.003 | 0.0051 | 0.0027 |
Lipid Particles | 0.0087 | 0 | 0.0024 | 0.0047 | 0.0023 | 0.0045 | 0.0242 | 0.0012 | 0.0092 | 0.0031 | 0.0081 | 0.0025 | 0.0049 | 0.0002 | 0.0041 | 0.0092 | 0.0197 | 0.0009 |
Mitochondria | 0.0057 | 0.0001 | 0.0017 | 0.0065 | 0.0004 | 0.0012 | 0.0056 | 0.0025 | 0.0025 | 0.0165 | 0.0004 | 0.0032 | 0.017 | 0.0025 | 0.0044 | 0.0012 | 0.0183 | 0.0028 |
None | 0.1043 | 0.0464 | 0.0452 | 0.0863 | 0.0441 | 0.036 | 0.0699 | 0.027 | 0.0311 | 0.0317 | 0.0215 | 0.0533 | 0.0976 | 0.077 | 0.0746 | 0.068 | 0.0943 | 0.0547 |
Nuclear Periphery | 0.0091 | 0.0039 | 0.0033 | 0.0166 | 0.003 | 0.0039 | 0.0098 | 0.0042 | 0.0023 | 0.0033 | 0.0033 | 0.0034 | 0.0086 | 0.0025 | 0.0041 | 0.0026 | 0.0041 | 0.0043 |
Nucleolus | 0.0006 | 0.0001 | 0.0006 | 0.0015 | 0.0001 | 0.0002 | 0.0015 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0048 | 0.0011 | 0.001 | 0.0006 | 0.0059 | 0.0003 |
Nucleus | 0.0286 | 0.1052 | 0.0636 | 0.0525 | 0.0279 | 0.0607 | 0.0232 | 0.0867 | 0.0592 | 0.0177 | 0.0469 | 0.0463 | 0.0176 | 0.0209 | 0.0255 | 0.0098 | 0.0152 | 0.0301 |
Peroxisomes | 0.0061 | 0 | 0.0066 | 0.0016 | 0.0112 | 0.0043 | 0.0144 | 0.001 | 0.0057 | 0.0031 | 0.0105 | 0.0006 | 0.0017 | 0.0004 | 0.0044 | 0.0177 | 0.0049 | 0.0018 |
Punctate Nuclear | 0.0056 | 0.0009 | 0.0038 | 0.0059 | 0.0021 | 0.0036 | 0.0131 | 0.0017 | 0.001 | 0.004 | 0.0091 | 0.0023 | 0.0145 | 0.0057 | 0.0093 | 0.0232 | 0.008 | 0.0084 |
Vacuole | 0.0021 | 0.0007 | 0.0061 | 0.0021 | 0.0016 | 0.0013 | 0.0056 | 0.0011 | 0.002 | 0.0051 | 0.0026 | 0.0016 | 0.012 | 0.001 | 0.0021 | 0.0041 | 0.0031 | 0.0012 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0007 | 0.0013 | 0.0003 | 0.0003 | 0.0016 | 0.0003 | 0.0003 | 0.0017 | 0.0004 | 0.0004 | 0.0039 | 0.0002 | 0.0005 | 0.0014 | 0.0013 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.7194 | 1.5732 | 7.0042 | 10.0396 | 9.6971 | 6.1559 | 10.9356 | 11.5408 | 12.0485 | 10.5256 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|