Standard name | |
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Human Ortholog | |
Description | Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 2 | 1 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | – | 3 | 2 | 1 | 3 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 7 | 6 | 5 | 5 | 13 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 46 | 25 | 25 | 45 | 36 | 69 | 57 | 32 | 49 | 39 | 58 | 43 | 44 | 44 | 63 | – | – | – | 3 | 3 | 2 | 7 | 0 | 3 |
Endoplasmic Reticulum | 2 | 1 | 3 | 1 | 1 | 9 | 2 | 15 | 9 | 13 | 9 | 6 | 3 | 4 | 2 | – | – | – | 0 | 0 | 1 | 0 | 2 | 0 |
Endosome | 19 | 5 | 55 | 29 | 37 | 7 | 12 | 10 | 0 | 0 | 0 | 1 | 9 | 8 | 10 | – | – | – | 27 | 17 | 39 | 25 | 27 | 28 |
Golgi | 0 | 1 | 1 | 0 | 2 | 2 | 3 | 8 | 4 | 4 | 0 | 4 | 0 | 0 | 0 | – | – | – | 4 | 3 | 1 | 0 | 1 | 0 |
Mitochondria | 7 | 12 | 43 | 47 | 34 | 127 | 143 | 165 | 177 | 163 | 179 | 159 | 0 | 0 | 0 | – | – | – | 3 | 3 | 6 | 10 | 6 | 8 |
Nucleus | 33 | 13 | 6 | 1 | 9 | 2 | 4 | 4 | 6 | 2 | 2 | 3 | 7 | 7 | 7 | – | – | – | 11 | 5 | 1 | 1 | 4 | 2 |
Nuclear Periphery | 2 | 6 | 13 | 0 | 3 | 29 | 25 | 48 | 42 | 37 | 13 | 34 | 0 | 0 | 0 | – | – | – | 9 | 3 | 26 | 10 | 7 | 4 |
Nucleolus | 4 | 1 | 5 | 3 | 1 | 2 | 2 | 1 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
SpindlePole | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 263 | 160 | 214 | 111 | 189 | 202 | 218 | 223 | 177 | 167 | 124 | 147 | 86 | 83 | 92 | – | – | – | 299 | 165 | 205 | 165 | 128 | 171 |
Unique Cell Count | 327 | 194 | 283 | 180 | 246 | 356 | 379 | 405 | 364 | 348 | 325 | 347 | 129 | 127 | 149 | 368 | 207 | 294 | 232 | 184 | 227 | |||
Labelled Cell Count | 377 | 225 | 366 | 238 | 312 | 451 | 467 | 516 | 472 | 434 | 394 | 416 | 150 | 146 | 174 | 368 | 207 | 294 | 232 | 184 | 227 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.9 | 5.6 | 6.1 | 6.9 | 6.9 | 7.4 | 8.1 | 8.3 | 8.5 | 8.4 | 9.6 | 6.5 | 6.5 | 6.4 | – | – | – | 6.6 | 7.3 | 7.5 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.3 | 1.8 | 1.6 | 1.7 | 1.8 | 1.9 | 1.8 | 2.3 | 2.5 | 3.0 | 1.6 | 1.6 | 0.9 | – | – | – | 2.7 | 3.5 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.29 | 0.3 | 0.4 | 0.53 | 0.56 | 0.6 | 0.58 | 0.76 | 0.2 | 0.2 | 0.18 | – | – | – | 0.22 | 0.37 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 4.7 | 2.1 | 3.7 | 2.4 | -0.4 | 1.8 | 1.0 | 3.2 | 1.4 | 6.4 | 6.5 | 8.2 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 1.4 | 0 | 2.1 | 1.3 | 2.1 | 1.5 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | -0.9 | -1.3 | -7.4 | -6.9 | -7.5 | -8.8 | -8.6 | -8.3 | -8.4 | -3.2 | -3.4 | -3.5 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 2.9 | -0.4 | 5.8 | 6.4 | 7.2 | 8.9 | 8.4 | 10.2 | 8.2 | -4.7 | -4.6 | -5.0 | – | – | – |
Nucleus | 0 | 1.1 | 0 | 0 | 0 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | -2.9 | -2.3 | 1.8 | 1.1 | 3.3 | 3.1 | 2.8 | -0.4 | 2.5 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | -3.2 | 0.3 | -5.0 | -4.8 | -5.5 | -7.0 | -7.1 | -9.3 | -8.4 | -1.9 | -2.2 | -3.0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0005 | 0 | 0.0442 | 0.0315 | 0.1053 | 0.0042 | 0.0871 | 0.0001 | 0.0064 | 0.0019 | 0.009 | 0.0011 | – | – | – | – | – | – |
Bud | 0.0005 | 0.0005 | 0.0006 | 0.002 | 0.0007 | 0.0011 | 0.0013 | 0.0011 | 0.0003 | 0.001 | 0.0003 | 0.0004 | – | – | – | – | – | – |
Bud Neck | 0.0007 | 0.0002 | 0.0021 | 0.0013 | 0.0011 | 0.0002 | 0.0013 | 0.0004 | 0.0007 | 0.0042 | 0.0005 | 0.0008 | – | – | – | – | – | – |
Bud Periphery | 0.0013 | 0.0004 | 0.0011 | 0.0044 | 0.0015 | 0.003 | 0.002 | 0.0015 | 0.0008 | 0.0019 | 0.0003 | 0.0009 | – | – | – | – | – | – |
Bud Site | 0.0003 | 0.0013 | 0.0215 | 0.0023 | 0.0029 | 0.0003 | 0.0029 | 0.003 | 0.0011 | 0.0022 | 0.0014 | 0.0005 | – | – | – | – | – | – |
Cell Periphery | 0.0008 | 0.0005 | 0.0008 | 0.0007 | 0.0003 | 0.0006 | 0.0011 | 0.001 | 0.0007 | 0.0014 | 0.0001 | 0.0007 | – | – | – | – | – | – |
Cytoplasm | 0.004 | 0.0271 | 0.0049 | 0.004 | 0.0017 | 0.0023 | 0.0073 | 0.0108 | 0.0015 | 0.0031 | 0.0021 | 0.015 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0038 | 0.0047 | 0.0181 | 0.0056 | 0.0101 | 0.001 | 0.0258 | 0.0147 | 0.0072 | 0.0802 | 0.0734 | 0.0028 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0129 | 0.0043 | 0.0015 | 0.0011 | 0.0013 | 0.0032 | 0.0047 | 0.0038 | 0.0038 | 0.0012 | 0.0014 | 0.0017 | – | – | – | – | – | – |
Endosome | 0.213 | 0.1018 | 0.1099 | 0.0936 | 0.0322 | 0.0719 | 0.1285 | 0.0962 | 0.1079 | 0.1012 | 0.2235 | 0.1081 | – | – | – | – | – | – |
Golgi | 0.0105 | 0.0039 | 0.0115 | 0.0172 | 0.0067 | 0.0049 | 0.0239 | 0.0032 | 0.0172 | 0.01 | 0.0653 | 0.0063 | – | – | – | – | – | – |
Lipid Particles | 0.0152 | 0.0006 | 0.0065 | 0.0088 | 0.0069 | 0.0011 | 0.021 | 0.0144 | 0.0181 | 0.0143 | 0.1107 | 0.0158 | – | – | – | – | – | – |
Mitochondria | 0.0344 | 0.0017 | 0.0521 | 0.0362 | 0.0025 | 0.0121 | 0.0149 | 0.0076 | 0.0204 | 0.0132 | 0.0197 | 0.0325 | – | – | – | – | – | – |
None | 0.0008 | 0.001 | 0.0017 | 0.0006 | 0.0031 | 0.0097 | 0.0052 | 0.0127 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | – | – | – | – | – | – |
Nuclear Periphery | 0.1471 | 0.0269 | 0.009 | 0.0033 | 0.0043 | 0.0253 | 0.0234 | 0.0175 | 0.0216 | 0.0062 | 0.0098 | 0.0179 | – | – | – | – | – | – |
Nucleolus | 0.0015 | 0.0015 | 0.0009 | 0.0089 | 0.0004 | 0.0006 | 0.0076 | 0.002 | 0.0021 | 0.0073 | 0.0004 | 0.0027 | – | – | – | – | – | – |
Nucleus | 0.014 | 0.0176 | 0.0027 | 0.0476 | 0.001 | 0.0025 | 0.0115 | 0.0155 | 0.0057 | 0.0028 | 0.0007 | 0.0048 | – | – | – | – | – | – |
Peroxisomes | 0.0009 | 0.0001 | 0.0126 | 0.0025 | 0.0089 | 0.0001 | 0.0189 | 0.0005 | 0.0033 | 0.0188 | 0.0479 | 0.0007 | – | – | – | – | – | – |
Punctate Nuclear | 0.0011 | 0.0002 | 0.006 | 0.0078 | 0.0049 | 0.0001 | 0.0071 | 0.0003 | 0.0006 | 0.001 | 0.0017 | 0.0002 | – | – | – | – | – | – |
Vacuole | 0.2718 | 0.4525 | 0.3942 | 0.4258 | 0.3689 | 0.3803 | 0.3494 | 0.3885 | 0.3999 | 0.3746 | 0.1537 | 0.3576 | – | – | – | – | – | – |
Vacuole Periphery | 0.265 | 0.3532 | 0.2977 | 0.2945 | 0.4349 | 0.4752 | 0.2546 | 0.405 | 0.3803 | 0.3528 | 0.2775 | 0.4289 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.8939 | 14.2831 | 20.3661 | 31.4675 | 16.4508 | 20.9788 | 24.2818 | 32.586 | 37.3614 | 26.9661 |
Translational Efficiency | 0.5223 | 0.5406 | 0.581 | 0.4722 | 0.4999 | 0.6394 | 0.4324 | 0.3189 | 0.4077 | 0.3653 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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