Standard name | |
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Human Ortholog | |
Description | Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 3 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 5 | 2 | 6 | 5 | 17 | 24 | 33 | 19 | 37 | 29 | 22 | 7 | 21 | 30 | 39 | 34 | 34 | 3 | 2 | 1 | 1 | 1 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 13 | 13 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 |
Golgi | 3 | 5 | 10 | 2 | 2 | 17 | 23 | 30 | 1 | 29 | 9 | 16 | 6 | 5 | 12 | 15 | 50 | 45 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 3 | 2 | 0 | 1 | 0 | 1 | 1 | 0 |
Nucleus | 277 | 239 | 131 | 114 | 60 | 81 | 104 | 144 | 53 | 108 | 103 | 59 | 61 | 77 | 70 | 197 | 173 | 106 | 346 | 352 | 160 | 89 | 98 | 85 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 3 |
Nucleolus | 10 | 16 | 9 | 8 | 8 | 4 | 18 | 12 | 17 | 12 | 26 | 21 | 9 | 2 | 7 | 17 | 11 | 6 | 21 | 27 | 19 | 10 | 17 | 19 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 60 | 75 | 21 | 31 | 41 | 69 | 125 | 108 | 40 | 69 | 62 | 68 | 34 | 39 | 55 | 80 | 65 | 91 | 33 | 44 | 15 | 14 | 29 | 44 |
Unique Cell Count | 345 | 325 | 163 | 157 | 115 | 177 | 269 | 302 | 126 | 237 | 217 | 177 | 111 | 139 | 163 | 341 | 307 | 250 | 421 | 443 | 203 | 121 | 151 | 165 |
Labelled Cell Count | 354 | 342 | 173 | 161 | 118 | 190 | 299 | 329 | 136 | 258 | 233 | 189 | 117 | 145 | 174 | 367 | 350 | 297 | 421 | 443 | 203 | 121 | 151 | 165 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 46.4 | 34.4 | 40.1 | 37.3 | 26.4 | 39.3 | 36.6 | 36.8 | 31.4 | 34.0 | 32.3 | 31.0 | 46.8 | 45.3 | 43.2 | 37.6 | 46.8 | 47.0 | 41.8 | 41.6 | 41.0 |
Std Deviation (1e-4) | 12.1 | 8.5 | 7.3 | 8.0 | 10.3 | 7.7 | 8.0 | 8.4 | 8.2 | 8.6 | 8.8 | 8.6 | 12.0 | 14.4 | 13.1 | 12.6 | 15.1 | 18.1 | 12.8 | 13.3 | 13.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.6 | -0.03 | -0.13 | -0.13 | -0.36 | -0.24 | -0.31 | -0.37 | 0.22 | 0.18 | 0.11 | -0.09 | 0.22 | 0.23 | 0.06 | 0.05 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 3.4 | 3.3 | 3.8 | 4.5 | 4.8 | 4.3 | 4.0 | 0 | 4.5 | 5.2 | 3.9 | 3.8 | 4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.3 | 0 | 1.2 | 0.9 | 1.4 | 0 | 2.0 | -0.9 | 1.0 | -0.3 | -1.0 | 0.4 | -0.8 | 3.1 | 3.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.6 | -5.0 | -6.6 | -8.4 | -6.8 | -6.7 | -7.0 | -6.5 | -8.7 | -4.5 | -4.7 | -6.9 | -5.0 | -5.2 | -7.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | 0.5 | -1.6 | 0.5 | -0.8 | 2.3 | -0.2 | 2.2 | 2.1 | 0.8 | -1.9 | -0.5 | -0.3 | -1.0 | -1.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.7 | 4.5 | 5.4 | 7.2 | 5.3 | 3.9 | 3.8 | 3.7 | 5.4 | 3.6 | 3.3 | 4.5 | 2.8 | 2.2 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 35.1222 | 40.7754 | 36.1333 | 36.1814 | 37.216 | 37.1078 | 41.9296 | 44.1209 | 43.7532 | 38.3112 | 44.1519 | 45.0415 | 42.7339 | 46.0173 | 44.3191 | 44.6845 | 42.7783 | 42.1799 |
Actin | 0.0071 | 0 | 0.0078 | 0.0328 | 0 | 0.0001 | 0.005 | 0.0001 | 0.0004 | 0.0027 | 0.0011 | 0.0001 | 0.0042 | 0 | 0.0023 | 0 | 0.0001 | 0.0001 |
Bud | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.001 |
Bud Periphery | 0.0011 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 |
Bud Site | 0.0012 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0013 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0006 | 0 | 0.0002 | 0 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 |
Cytoplasm | 0.0054 | 0.0028 | 0.0011 | 0.0003 | 0.0003 | 0.0003 | 0.0056 | 0.0016 | 0.0024 | 0.0016 | 0.0039 | 0.0003 | 0.0011 | 0.0007 | 0.0005 | 0.0003 | 0.0021 | 0.0006 |
Cytoplasmic Foci | 0.0126 | 0 | 0.0059 | 0.0007 | 0 | 0 | 0.0135 | 0.0001 | 0.0004 | 0.0054 | 0.001 | 0.0001 | 0.0047 | 0.0038 | 0.0002 | 0 | 0.0058 | 0.0023 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0014 | 0.0007 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0075 | 0 | 0.0023 | 0.0002 | 0 | 0 | 0.0089 | 0 | 0.0004 | 0.0062 | 0.001 | 0.0041 | 0.0045 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0003 |
Golgi | 0.0039 | 0 | 0.001 | 0.001 | 0 | 0 | 0.0027 | 0 | 0 | 0.0024 | 0.0002 | 0.0003 | 0.0012 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
Lipid Particles | 0.0131 | 0 | 0.0041 | 0.0011 | 0 | 0 | 0.0054 | 0 | 0.0002 | 0.0053 | 0.0002 | 0.0002 | 0.0042 | 0.0013 | 0.0003 | 0 | 0.0037 | 0.0007 |
Mitochondria | 0.0108 | 0.0002 | 0.0061 | 0.0013 | 0.0003 | 0.0003 | 0.0019 | 0.0005 | 0.0019 | 0.0019 | 0.0009 | 0.0005 | 0.0019 | 0.0009 | 0.0011 | 0.0002 | 0.0005 | 0.0003 |
None | 0.0022 | 0 | 0.0007 | 0 | 0 | 0 | 0.0019 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0017 | 0.0006 |
Nuclear Periphery | 0.0225 | 0.0256 | 0.0187 | 0.018 | 0.0089 | 0.0214 | 0.0254 | 0.0244 | 0.0278 | 0.0432 | 0.0348 | 0.0085 | 0.0092 | 0.008 | 0.0048 | 0.0029 | 0.0086 | 0.0074 |
Nucleolus | 0.0391 | 0.0097 | 0.0189 | 0.003 | 0.0597 | 0.0382 | 0.0331 | 0.0082 | 0.0206 | 0.0213 | 0.0573 | 0.0176 | 0.0245 | 0.0073 | 0.0055 | 0.0024 | 0.0179 | 0.0212 |
Nucleus | 0.8554 | 0.9602 | 0.9232 | 0.9379 | 0.926 | 0.9361 | 0.8828 | 0.9574 | 0.9389 | 0.892 | 0.8853 | 0.9638 | 0.9369 | 0.9764 | 0.9818 | 0.994 | 0.9521 | 0.9574 |
Peroxisomes | 0.002 | 0 | 0.0046 | 0.0027 | 0 | 0 | 0.0026 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0018 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0042 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0051 | 0.0064 | 0.0033 | 0.0084 | 0.0086 | 0.0002 | 0.001 | 0.0003 | 0.0002 | 0 | 0.0056 | 0.005 |
Vacuole | 0.0041 | 0.0009 | 0.0014 | 0.0001 | 0.0023 | 0.0011 | 0.0013 | 0.0003 | 0.0009 | 0.0018 | 0.0014 | 0.0016 | 0.0015 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0018 |
Vacuole Periphery | 0.0041 | 0.0004 | 0.0018 | 0.0002 | 0.0012 | 0.0009 | 0.0019 | 0.0006 | 0.0014 | 0.0041 | 0.0022 | 0.0016 | 0.0015 | 0.0003 | 0.0003 | 0 | 0.0008 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 155.5524 | 109.1642 | 163.9377 | 158.8188 | 131.9405 | 132.219 | 166.5492 | 168.5033 | 158.0918 | 144.5308 |
Translational Efficiency | 1.3612 | 1.9754 | 1.2777 | 1.4902 | 1.4095 | 1.7867 | 1.4321 | 1.4164 | 1.3701 | 1.3628 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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