Standard name
Human Ortholog
Description Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0 0 0.05 – 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0 0
Cytoplasm 0.97 0.93 0.94 0.97 0.92 0.93 0.84 0.82 0.73 – 0.64 0.74 0.95 0.97 0.97 0.83 0.9 0.95 0.89 0.93 0.87 0.72 0.75 0.8
Endoplasmic Reticulum 0 0.06 0 0 0.05 0 0.07 0.09 0.07 – 0 0.06 0 0 0 0 0 0 0 0 0 0.19 0.15 0.14
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.09 0.06 0.24 – 0.4 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 2 4 6 7 7 – 3 7 0 1 1 0 0 0 0 0 1 0 1 1
Bud 0 1 0 1 0 0 3 2 0 – 8 4 1 0 2 0 0 0 0 0 2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 6 19 6 8 2 12 7 14 – 12 13 4 3 11 9 11 0 0 0 0 0 0 0
Cytoplasm 92 186 254 201 236 288 344 234 204 – 173 216 350 342 400 96 111 100 105 192 244 178 259 277
Endoplasmic Reticulum 2 11 6 5 14 8 27 27 19 – 11 17 16 8 7 5 5 3 4 2 10 46 51 47
Endosome 0 1 0 0 0 1 1 0 0 – 1 0 0 0 1 0 0 0 1 1 1 0 0 0
Golgi 0 0 1 0 0 0 3 1 0 – 0 0 0 0 0 1 1 0 0 0 2 0 3 1
Mitochondria 1 0 0 1 1 17 37 17 66 – 108 67 2 0 4 1 2 0 1 0 6 4 5 3
Nucleus 0 0 2 1 0 2 2 1 1 – 4 2 0 1 1 1 2 1 0 0 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 1 – 1 0 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 0 5 4 8 12 7 – 6 9 1 0 3 11 9 5 2 5 6 9 16 11
Unique Cell Count 95 199 269 208 256 311 410 285 278 269 290 367 351 412 115 124 105 118 207 282 247 347 348
Labelled Cell Count 97 205 287 216 266 326 443 308 319 327 335 374 355 431 124 141 110 118 207 282 247 347 348


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 11.4 10.8 9.7 10.0 8.2 8.2 8.2 7.2 – 6.8 7.3 11.1 11.4 11.3 14.6 14.2 13.7 10.8 11.1 11.7
Std Deviation (1e-4) 1.9 1.8 1.3 1.3 1.3 1.2 1.2 1.2 1.2 – 1.4 1.2 1.4 1.6 1.4 2.6 2.3 2.1 1.7 1.7 1.6
Intensity Change (Log2) – – – -0.14 -0.1 -0.4 -0.38 -0.39 -0.58 – -0.67 -0.57 0.05 0.09 0.07 0.44 0.4 0.35 0.01 0.04 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -2.0 -2.0 -4.1 -2.5 -2.6 -1.0 – -1.3 -1.3 -4.0 -4.1 -2.7 0.3 0.6 -2.8
Cytoplasm 1.1 -1.0 -0.9 -4.1 -4.5 -6.7 – -8.6 -6.4 0.5 1.9 1.7 -3.5 -1.8 0.3
Endoplasmic Reticulum 0 1.9 0.3 2.6 3.6 2.6 – 1.2 2.2 1.5 0 -0.5 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 5.1 4.1 8.5 – 11.6 8.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.2 0 – 0 1.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.7694 10.6345 9.6178 9.0902 8.7963 10.1289 8.8704 12.4871 11.3222 8.9358 10.8126 11.2697 10.5339 12.2901 12.4104 10.4593 10.972 10.945
Actin 0.0179 0.0013 0.0042 0.0004 0.0018 0.0015 0.0097 0.0014 0.0017 0.0047 0.0015 0.001 0.0023 0.0026 0.0042 0.0005 0.0153 0.0015
Bud 0.0009 0.0009 0.0013 0.0011 0.0014 0.0009 0.001 0.0025 0.0012 0.0011 0.0005 0.0006 0.0011 0.0014 0.0063 0.0018 0.0022 0.0015
Bud Neck 0.0006 0.0004 0.0012 0.0009 0.0008 0.0012 0.0009 0.0002 0.0004 0.0007 0.0008 0.001 0.0008 0.001 0.0027 0.003 0.0015 0.0045
Bud Periphery 0.0012 0.0005 0.0007 0.0004 0.0008 0.0008 0.0009 0.0016 0.0008 0.0007 0.0002 0.0004 0.0007 0.0005 0.003 0.0009 0.0013 0.0012
Bud Site 0.0016 0.0019 0.0022 0.0006 0.0014 0.0004 0.0028 0.0041 0.0008 0.0008 0.0005 0.0001 0.0026 0.017 0.0175 0.0017 0.0037 0.0007
Cell Periphery 0.0013 0.0005 0.0006 0.0002 0.0003 0.0002 0.0005 0.0001 0.0002 0.0002 0.0002 0.0002 0.0005 0.0003 0.0011 0.0002 0.0003 0.0002
Cytoplasm 0.6822 0.8893 0.7626 0.6994 0.6124 0.767 0.6721 0.9137 0.825 0.6215 0.7737 0.8181 0.8355 0.905 0.7985 0.7566 0.7571 0.8647
Cytoplasmic Foci 0.0252 0.0133 0.0222 0.0204 0.042 0.0286 0.0374 0.0159 0.02 0.0421 0.025 0.0227 0.0265 0.0205 0.0334 0.0676 0.0535 0.0304
Eisosomes 0.0005 0 0.0002 0 0 0 0.0001 0 0 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0703 0.0242 0.0273 0.0478 0.013 0.0272 0.0299 0.0033 0.0114 0.0106 0.0359 0.0373 0.0227 0.0036 0.0143 0.006 0.0121 0.0162
Endosome 0.092 0.0421 0.1102 0.1285 0.2101 0.1099 0.1188 0.032 0.0826 0.1791 0.0769 0.0797 0.0629 0.0211 0.0548 0.0883 0.0888 0.0453
Golgi 0.0194 0.006 0.0146 0.0049 0.0283 0.0153 0.0291 0.0071 0.0079 0.0344 0.0062 0.0179 0.0077 0.0026 0.0038 0.0049 0.0054 0.0048
Lipid Particles 0.005 0.0001 0.0029 0.0001 0.0097 0.0004 0.0187 0.0001 0.0022 0.0027 0.0011 0.0001 0.0023 0.0012 0.002 0.0025 0.015 0.0014
Mitochondria 0.0239 0.003 0.0082 0.003 0.0212 0.026 0.0236 0.0119 0.019 0.0581 0.0341 0.0099 0.0071 0.0015 0.0022 0.0028 0.0063 0.0038
None 0.0104 0.0011 0.0044 0.0008 0.0021 0.0027 0.0013 0.0004 0.0011 0.0006 0.0026 0.0007 0.0068 0.0106 0.0173 0.0098 0.0078 0.0098
Nuclear Periphery 0.0089 0.0014 0.0026 0.0055 0.0143 0.0014 0.0092 0.0003 0.0027 0.0019 0.0039 0.001 0.0019 0.0006 0.003 0.0031 0.0035 0.0015
Nucleolus 0.0007 0 0.0004 0.0001 0.0003 0 0.0002 0 0 0.0001 0.0051 0 0.0002 0.0002 0.001 0.0005 0.0011 0.0001
Nucleus 0.0031 0.0012 0.0015 0.0016 0.0113 0.001 0.0016 0.0004 0.001 0.0012 0.0087 0.0007 0.0023 0.0017 0.0086 0.0125 0.0034 0.0026
Peroxisomes 0.0053 0.0001 0.0052 0.0001 0.0012 0.0003 0.0089 0.0002 0.0011 0.0039 0.0006 0.0002 0.0007 0.0008 0.0006 0.0009 0.0021 0.0018
Punctate Nuclear 0.0062 0.0001 0.0007 0.0001 0.0067 0.0001 0.001 0 0.0004 0.0005 0.0098 0.0001 0.0006 0.0007 0.0026 0.0037 0.0038 0.001
Vacuole 0.0186 0.0101 0.0209 0.0637 0.0138 0.0091 0.0231 0.0033 0.015 0.025 0.0094 0.0054 0.0124 0.0064 0.0208 0.0277 0.0133 0.0056
Vacuole Periphery 0.0048 0.0026 0.0056 0.0205 0.0068 0.0057 0.0091 0.0012 0.0055 0.01 0.0033 0.0029 0.0025 0.0008 0.0022 0.0049 0.0021 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.7489 60.2847 48.3016 53.5427 36.2078 35.5606 59.5515 47.3233 38.1405 53.0536
Translational Efficiency 1.1291 1.0458 1.0628 0.5851 1.0652 1.4895 0.712 0.8233 0.8538 0.7813

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Puf4

Puf4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Puf4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available